FastQCFastQC Report
Wed 25 May 2016
SRR1780178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343870
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT73292.131328699799343No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC21060.6124407479570768No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG18810.5470090441155088No Hit
CACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTG17590.5115305202547474RNA PCR Primer, Index 32 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4790.1392968272893826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGT150.002223574870.0000149
TCTTTAT950.055.263161
GTATGTA200.006947914652.51
CTTTATA1050.049.9999962
TTTATAC1300.043.0769233
TTGAAAA17800.037.5561858
CTTGAAA18050.037.2299257
GAAAAAA18200.037.11538760
TATGCCG18800.037.04787443
GTATGCC19000.036.84210642
TGAAAAA18100.036.7403359
CGTATGC19100.036.64921641
TCGTATG19150.036.55352840
CTCGTAT18900.036.4814839
GCTTGAA18750.036.21333356
TCTGCTT18950.036.20052753
TGCTTGA18850.036.02121755
TTCTGCT19250.036.052
TCTTCTG19100.035.9162350
GTCTTCT19100.035.9162349