FastQCFastQC Report
Wed 25 May 2016
SRR1780177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420173
Sequences flagged as poor quality0
Sequence length50
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGC207.853605E-444.00398310
CCAATAG250.002341736635.23423420
CGAAACT407.044962E-427.4918325
GAAACTC703.223244E-521.99346426
AAACTCG654.942637E-420.30165927
GGTCTAG809.049582E-519.233441
TTATCGA708.1578875E-418.84556641
TCCACAA708.171419E-418.8409212
CTTAAAC600.007449607418.3175621
TATATAG600.007449607418.3175622
ATAGAGG600.007452633718.3162733
ATATAGG600.007452633718.3162733
GCTACAG600.007452633718.3162733
TAGATCT1802.8376235E-1017.12352239
CTCATAC902.2303274E-417.0963921
TGTATAA1259.519912E-615.8263732
AAGTATG850.002986188315.52479927
GAAGTAT850.002986188315.52479926
ACGAAAC1004.925187E-415.4079124
GGTCCAA1057.2067644E-414.654051
Produced by FastQC (version 0.11.5)