FastQCFastQC Report
Wed 25 May 2016
SRR1780177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420173
Sequences flagged as poor quality0
Sequence length50
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT153941.0839524480468223No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC47040.3312272518911428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG39730.2797546496095898No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG31000.21828326548948615Illumina PCR Primer Index 4 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT250.002331073435.2670251
CTTTATA756.184564E-1132.2689132
TTAATAC454.0681076E-529.3353752
TTATATA300.005743392329.3353752
GCTTATA300.005743392329.3353753
TCTTTAT852.582965E-1028.5248011
CTTAATA554.888958E-628.0533181
ATGCCGT24650.026.1500844
TATGCCG25050.025.73251543
TTTATCG450.001398340624.44442640
TAATACA551.5930564E-424.0016733
TATATAC651.812704E-523.6939563
TTTATAC1053.0686351E-923.0492253
ATATACA500.00257961522.0015324
GTCTTCT6050.021.81816543
TCTTCTG6300.021.3015744
CTCGTAT30950.020.8263939
GTATGCC31150.020.76402942
TCGTATG31150.020.76402940
CGTATGC31350.020.70173841
Produced by FastQC (version 0.11.5)