FastQCFastQC Report
Wed 25 May 2016
SRR1780176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147445
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG150.002221047970.0000170
TTGCGCG150.002221047970.0000119
TTTAGGC200.006940081252.524
GATACAG200.006940081252.562
TGGAATT200.006940081252.521
CACGTAA200.006940081252.561
TGACTAA200.006940081252.531
GAGTGTT350.001252211440.020
GCATTGT350.001252211440.050
CTTACGC556.6595694E-638.181829
TTACGCT501.7032736E-435.010
TCTTACG552.9809E-431.8181848
CGCTACC901.7411367E-731.11110916
ACGCTAC450.00429640531.11110915
GCTACCT755.555567E-528.017
GGGGAAC500.00719031427.9999987
CTATAGT500.00719031427.9999984
AGATCTC1101.0068688E-625.45454840
TAGATCT1101.0068688E-625.45454839
CAACTGA700.001221487525.06
Produced by FastQC (version 0.11.5)