FastQCFastQC Report
Wed 25 May 2016
SRR1780176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147445
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT105347.144358913493167No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC30532.070602597578758No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG26321.7850723998779203No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG22651.5361660280104446Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6230.4225304350774865No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3720.25229746685204657RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT2580.1749805012038387No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1760.11936654345688223No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1700.11529722947539761No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA150.002221047970.0000157
CATTAGA150.002221047970.000012
TGAAATT252.3841819E-455.99999648
TCTTGTC200.006940081252.522
GCCTTAT200.006940081252.512
GATGTTA200.006940081252.553
CAAGTAG200.006940081252.531
CCAGGTA200.006940081252.53
ACATTAG200.006940081252.51
TGCTCAA200.006940081252.520
ATTTCGG200.006940081252.548
TCTTTAT900.050.5555531
TATGAAT305.862646E-446.6666682
GTATGAA305.862646E-446.6666681
AAACTCG451.6757349E-646.66666436
AACTCGT451.6757349E-646.66666437
CTTTATA1100.044.5454562
TAATACA602.5792906E-740.8333364
TTAATAC350.001252211440.03
ATGGCTC350.001252211440.07
Produced by FastQC (version 0.11.5)