##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147445 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74055410492048 34.0 34.0 34.0 34.0 34.0 2 33.72641323883482 34.0 34.0 34.0 34.0 34.0 3 33.72519244464038 34.0 34.0 34.0 34.0 34.0 4 33.761104140526975 34.0 34.0 34.0 34.0 34.0 5 33.736172810200415 34.0 34.0 34.0 34.0 34.0 6 37.486683169995594 38.0 38.0 38.0 37.0 38.0 7 37.474115771982774 38.0 38.0 38.0 37.0 38.0 8 37.37245752653532 38.0 38.0 38.0 37.0 38.0 9 37.38988097256604 38.0 38.0 38.0 37.0 38.0 10-11 37.345990030180744 38.0 38.0 38.0 36.0 38.0 12-13 37.32903455525789 38.0 38.0 38.0 36.0 38.0 14-15 37.24871986164332 38.0 38.0 38.0 36.0 38.0 16-17 37.27565532910577 38.0 38.0 38.0 36.0 38.0 18-19 37.22175387432602 38.0 38.0 38.0 36.0 38.0 20-21 37.25960866763878 38.0 38.0 38.0 36.0 38.0 22-23 37.23692224219201 38.0 38.0 38.0 36.0 38.0 24-25 37.24954728881956 38.0 38.0 38.0 36.0 38.0 26-27 37.13807182339177 38.0 38.0 38.0 36.0 38.0 28-29 37.09371969208857 38.0 38.0 38.0 36.0 38.0 30-31 37.08821594492862 38.0 38.0 38.0 36.0 38.0 32-33 37.010593780731796 38.0 38.0 38.0 36.0 38.0 34-35 37.021852216080575 38.0 38.0 38.0 36.0 38.0 36-37 37.041686730645324 38.0 38.0 38.0 36.0 38.0 38-39 36.454610193631524 38.0 37.0 38.0 34.0 38.0 40-41 36.735982908881276 38.0 37.0 38.0 34.5 38.0 42-43 36.97696429176981 38.0 37.5 38.0 35.5 38.0 44-45 37.0527043982502 38.0 38.0 38.0 36.0 38.0 46-47 36.998490962731864 38.0 38.0 38.0 35.5 38.0 48-49 37.01717928719184 38.0 38.0 38.0 36.0 38.0 50-51 36.869988809386555 38.0 37.5 38.0 35.0 38.0 52-53 36.86218929092204 38.0 38.0 38.0 35.0 38.0 54-55 36.839536776425106 38.0 37.5 38.0 35.0 38.0 56-57 36.89732781715216 38.0 37.5 38.0 35.0 38.0 58-59 36.760829461833225 38.0 37.0 38.0 34.5 38.0 60-61 36.76629590694836 38.0 37.0 38.0 34.5 38.0 62-63 36.7401980399471 38.0 37.0 38.0 34.5 38.0 64-65 36.675662111295736 38.0 37.0 38.0 34.5 38.0 66-67 36.348438400759605 38.0 37.0 38.0 34.0 38.0 68-69 36.24612567398013 38.0 37.0 38.0 34.0 38.0 70-71 35.49480484248364 38.0 36.5 38.0 29.0 38.0 72-73 34.29409271253688 38.0 36.0 38.0 22.5 38.0 74-75 33.93214758045373 38.0 36.0 38.0 21.0 38.0 76 33.19132557902947 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.151194735335892 1101 2 -0.10031149640060733 1101 3 -0.022361343337095718 1101 4 -0.019965070727039347 1101 5 0.03754580594308976 1101 6 -0.19927260239195732 1101 7 0.0024155065406503695 1101 8 -0.1847750748108865 1101 9 -0.005149471504473979 1101 10-11 0.07393281521741102 1101 12-13 0.13718905697111694 1101 14-15 0.2661638432388358 1101 16-17 0.1433389628611863 1101 18-19 -0.06008738338868369 1101 20-21 -0.15483735474484916 1101 22-23 -0.15271617579644925 1101 24-25 0.026661520759539314 1101 26-27 -0.2749178127673986 1101 28-29 -0.5311119106374704 1101 30-31 -0.17799369983000446 1101 32-33 -0.11275923632639717 1101 34-35 -0.03974537185715832 1101 36-37 -0.11357350403692834 1101 38-39 0.07151879654885818 1101 40-41 -0.04051195336113267 1101 42-43 -0.015515685201854978 1101 44-45 -0.13470698655444835 1101 46-47 -0.05787137605479131 1101 48-49 -0.03893486451687522 1101 50-51 -0.12374973594341299 1101 52-53 -0.016055759106500034 1101 54-55 0.09657269148016212 1101 56-57 -0.18620204718823175 1101 58-59 0.024917917982527626 1101 60-61 -0.2072569201312433 1101 62-63 -0.23516082370918667 1101 64-65 -0.21585573537947766 1101 66-67 0.0023539024549990017 1101 68-69 -0.3135301915436344 1101 70-71 -0.3127924974264431 1101 72-73 -0.4323039665385906 1101 74-75 -0.38800017391668007 1101 76 -1.3814392115903864 1102 1 0.08079178805674303 1102 2 0.05238510126651619 1102 3 -0.10425470457653319 1102 4 -0.036759782179601075 1102 5 -0.009443652695466653 1102 6 0.17028762119720398 1102 7 -0.12540807241747132 1102 8 0.014119741289576382 1102 9 -0.03673527001898691 1102 10-11 0.1034958464331126 1102 12-13 0.09994971957097931 1102 14-15 0.015206697829306393 1102 16-17 0.059218643302237695 1102 18-19 0.2362512163713859 1102 20-21 0.09965484771054633 1102 22-23 0.04387511035174185 1102 24-25 0.02472808703227969 1102 26-27 0.15283685172121864 1102 28-29 0.20021737290728225 1102 30-31 0.2734726469373996 1102 32-33 0.3396642558505363 1102 34-35 0.19042257525736517 1102 36-37 0.12483865553835471 1102 38-39 0.13759373465345703 1102 40-41 0.22388989456760555 1102 42-43 0.2082649115464008 1102 44-45 0.2055837304112771 1102 46-47 0.1666111273271227 1102 48-49 0.1407851385459935 1102 50-51 0.22835853634531844 1102 52-53 0.38205930253366205 1102 54-55 0.21412290970931736 1102 56-57 0.2851154953728354 1102 58-59 0.271963529151769 1102 60-61 0.443831673247864 1102 62-63 0.41758682171310113 1102 64-65 0.2479641681402711 1102 66-67 0.0921596657465571 1102 68-69 0.19386535198733412 1102 70-71 0.18224948935218777 1102 72-73 0.23067875379627623 1102 74-75 0.20438988198061026 1102 76 0.38391978174788477 1103 1 -0.05133122606645202 1103 2 0.09597675081988655 1103 3 0.03246685061225918 1103 4 -0.003864465132245698 1103 5 0.058595856961474624 1103 6 -0.0393995520229069 1103 7 0.08560196878042348 1103 8 -0.09797757139861574 1103 9 -0.01782455730811705 1103 10-11 -0.012062970796925754 1103 12-13 0.12112820595803697 1103 14-15 0.035736348970125675 1103 16-17 0.12373995407542537 1103 18-19 0.27674987460529366 1103 20-21 0.24402698937081624 1103 22-23 0.15439128083792752 1103 24-25 -0.11610380794459729 1103 26-27 -0.02896615433877514 1103 28-29 -0.04107374623902871 1103 30-31 -0.03777929011928194 1103 32-33 0.0724606727681234 1103 34-35 0.18528543794366925 1103 36-37 0.0918582969781383 1103 38-39 -0.060625433073603574 1103 40-41 0.05561793810885263 1103 42-43 0.11802326766245841 1103 44-45 0.19755518166043373 1103 46-47 0.0643408339023992 1103 48-49 0.21583899817079555 1103 50-51 0.07475151632591093 1103 52-53 0.0959229589407542 1103 54-55 0.1264482596393961 1103 56-57 -0.1325863679812045 1103 58-59 0.29748858175152293 1103 60-61 0.015468123930084232 1103 62-63 0.16830180035823616 1103 64-65 0.164138433948537 1103 66-67 -0.01397529198465719 1103 68-69 -0.04914234584442312 1103 70-71 -0.17757060060283436 1103 72-73 0.22893067825422264 1103 74-75 0.019295454404485213 1103 76 0.3568828800306747 1104 1 -0.2554485573790046 1104 2 -0.07155821807910456 1104 3 0.055326880292412284 1104 4 0.07613932382295019 1104 5 -0.032389587135654097 1104 6 -0.028064261062723972 1104 7 -0.010921664827613142 1104 8 -0.22930907340360562 1104 9 0.1419304235892369 1104 10-11 0.0898030896369022 1104 12-13 -0.09407813422699718 1104 14-15 0.10352391488215318 1104 16-17 0.018103883221961325 1104 18-19 -0.01324096875298153 1104 20-21 0.036354355099710745 1104 22-23 -0.16777726451902453 1104 24-25 0.013030100046940163 1104 26-27 0.04615619733942111 1104 28-29 0.010860199642060309 1104 30-31 0.05097067041243264 1104 32-33 -0.16930308587542697 1104 34-35 0.035441732345496746 1104 36-37 -0.13769555354758012 1104 38-39 -0.11524637968091156 1104 40-41 -0.20710505769172016 1104 42-43 -0.02215228726159779 1104 44-45 -0.048101728446596326 1104 46-47 -0.12803705122910713 1104 48-49 -0.07364349369873935 1104 50-51 -0.29107526527239 1104 52-53 -0.2729002547247461 1104 54-55 -0.3362936809371604 1104 56-57 -0.23666580974180818 1104 58-59 -0.2020314839236974 1104 60-61 -0.1815164992267455 1104 62-63 -0.028828066081089787 1104 64-65 -0.2410524346694416 1104 66-67 -0.5276755224794343 1104 68-69 -0.14333892946981308 1104 70-71 -0.41621794359799935 1104 72-73 -0.04227326225917949 1104 74-75 -0.22019808858436818 1104 76 0.29608909391719607 1105 1 -0.17751187482186026 1105 2 0.05636012931118728 1105 3 0.13649055221515738 1105 4 -0.14703632707546888 1105 5 0.17474890449128822 1105 6 0.12408706518101553 1105 7 0.148685962601796 1105 8 -0.11998258519923155 1105 9 0.0021736701361092514 1105 10-11 0.007973239985275882 1105 12-13 0.07850773547108503 1105 14-15 -0.06762264421819708 1105 16-17 0.03000340650761757 1105 18-19 0.16301193431510086 1105 20-21 0.11506722638684153 1105 22-23 -0.02527940362182335 1105 24-25 0.028501925007212492 1105 26-27 0.14191771793592522 1105 28-29 0.17205076314285606 1105 30-31 0.2555850207363548 1105 32-33 0.1286158726870923 1105 34-35 0.02449563773603103 1105 36-37 0.15292600860863814 1105 38-39 -0.12181342527436101 1105 40-41 -0.12785741070479872 1105 42-43 0.0779730030452086 1105 44-45 0.08185793418633125 1105 46-47 0.21942047230102446 1105 48-49 0.02274753429295373 1105 50-51 -0.01110032333375699 1105 52-53 0.22360078426804364 1105 54-55 -0.0713657992601 1105 56-57 0.09169258165289307 1105 58-59 0.2250923395697626 1105 60-61 0.15167320863292844 1105 62-63 0.16611142786830158 1105 64-65 -0.17432159491879418 1105 66-67 0.009074263610287403 1105 68-69 -0.1158759053896361 1105 70-71 0.10240141833818939 1105 72-73 0.3294630780415204 1105 74-75 0.48058726772904237 1105 76 0.5593087492160578 1106 1 0.034513599082309554 1106 2 -0.029465036119923127 1106 3 -0.06142974232685816 1106 4 -0.00964143180704724 1106 5 -0.005284928037688985 1106 6 0.11084013747316845 1106 7 0.11312449188186235 1106 8 0.014849993536032002 1106 9 0.11622163272131303 1106 10-11 -0.015510515425447124 1106 12-13 -0.04113130129690745 1106 14-15 -0.061055153298255505 1106 16-17 0.06873667681957585 1106 18-19 0.023048503298632284 1106 20-21 0.06926892796403905 1106 22-23 0.023137184268122724 1106 24-25 0.05889522815662218 1106 26-27 -0.09501571158793354 1106 28-29 -0.00208725367389917 1106 30-31 0.012401280251751245 1106 32-33 0.18626177546832423 1106 34-35 -0.10515011578404909 1106 36-37 -0.02116847370391639 1106 38-39 0.2589455677103629 1106 40-41 0.04694606210479435 1106 42-43 0.004617363461150603 1106 44-45 -0.03924261807995322 1106 46-47 0.011686256668404837 1106 48-49 -0.04074443048141063 1106 50-51 0.07392256337702463 1106 52-53 0.05525285651044953 1106 54-55 0.11776674464755388 1106 56-57 0.15428276026874244 1106 58-59 0.3530091513514364 1106 60-61 0.28898725410782333 1106 62-63 0.21687265977038805 1106 64-65 0.2707938337094902 1106 66-67 0.2372199424997845 1106 68-69 0.3646144175120085 1106 70-71 0.5452281758883188 1106 72-73 0.7071497788723988 1106 74-75 0.22910087537120916 1106 76 -0.004450641798420918 1107 1 0.11994073476070355 1107 2 0.19666561714640807 1107 3 0.06922352400479781 1107 4 0.028047010404002037 1107 5 0.03742863980150446 1107 6 0.18119189626713705 1107 7 0.17593856223361826 1107 8 0.20831733021945098 1107 9 0.25190002468111317 1107 10-11 0.041022147891141 1107 12-13 0.11464759317545514 1107 14-15 0.1512564044906881 1107 16-17 0.17049547078942595 1107 18-19 0.006716845007176175 1107 20-21 0.01524882746152656 1107 22-23 0.0997703500972662 1107 24-25 0.06392035378475924 1107 26-27 0.1964415748442292 1107 28-29 0.17881726893916294 1107 30-31 -0.056694197135179536 1107 32-33 0.11088489104621146 1107 34-35 0.043091090246100805 1107 36-37 0.10194710418552688 1107 38-39 0.13457370841388894 1107 40-41 0.16503651436610056 1107 42-43 0.03728068004406282 1107 44-45 -0.011604427125178063 1107 46-47 0.1486209300352428 1107 48-49 0.22181838358893913 1107 50-51 0.13673663372878764 1107 52-53 0.10676039419888639 1107 54-55 0.15294262404454173 1107 56-57 -0.22637050606292064 1107 58-59 0.003762920195661934 1107 60-61 -0.09920370066603823 1107 62-63 0.1816967803734002 1107 64-65 0.23938679853361577 1107 66-67 0.36536064601737195 1107 68-69 0.1749159250496959 1107 70-71 0.13442415578781208 1107 72-73 -0.06169444223312581 1107 74-75 -0.1427584211112034 1107 76 -0.14012903375822106 1108 1 -0.04489861349573232 1108 2 -0.16723723042478156 1108 3 -0.1413622330633686 1108 4 -0.11190781422418894 1108 5 -0.17341091149030774 1108 6 -0.14871521504700524 1108 7 0.14396049005453904 1108 8 0.06491090414970557 1108 9 -0.1766442116520679 1108 10-11 -0.15144270160888595 1108 12-13 -0.25437555285774494 1108 14-15 -0.07649853180695487 1108 16-17 -0.06203079691272251 1108 18-19 0.025535686690339787 1108 20-21 0.12776849651385902 1108 22-23 0.06106673099164084 1108 24-25 0.030089583773090567 1108 26-27 0.013804906654797833 1108 28-29 0.01992305037158104 1108 30-31 -0.04883292105782999 1108 32-33 -0.1560875976599405 1108 34-35 -0.13198251007874973 1108 36-37 -0.07279656364858766 1108 38-39 -0.34852359629146434 1108 40-41 -0.16759888485221097 1108 42-43 -0.11292108636373399 1108 44-45 -0.08084583619070429 1108 46-47 0.08498202845664338 1108 48-49 0.1851387734168668 1108 50-51 0.020765363234900747 1108 52-53 -0.3681583916495441 1108 54-55 -0.16892555523570252 1108 56-57 -0.09295088269354324 1108 58-59 -0.1754602156857672 1108 60-61 -0.1373806967576101 1108 62-63 0.016121428868402177 1108 64-65 0.15069840708140703 1108 66-67 -0.07913455727180008 1108 68-69 0.015387220922995937 1108 70-71 0.2370648846848269 1108 72-73 -0.4144113093972308 1108 74-75 -0.3008658775854869 1108 76 0.6194897673178659 1109 1 0.08500507454748885 1109 2 -0.02188839321548386 1109 3 -0.006114405955081281 1109 4 0.042719857845348486 1109 5 0.02784584209202734 1109 6 -0.16645070952668561 1109 7 0.1329198511069265 1109 8 -0.03286922270018522 1109 9 0.12562264294037107 1109 10-11 0.049579144761800364 1109 12-13 0.04427960335555525 1109 14-15 0.03097151752355387 1109 16-17 0.05565771612462811 1109 18-19 0.20565079122405194 1109 20-21 0.007672184557080186 1109 22-23 -0.31314045716903394 1109 24-25 -0.021824910001868147 1109 26-27 0.12852771614504377 1109 28-29 0.052663242995492965 1109 30-31 0.02114006461282969 1109 32-33 0.03862111739761076 1109 34-35 0.09332286929639366 1109 36-37 0.18375147487619614 1109 38-39 -0.07197908137485598 1109 40-41 -0.04356787710027277 1109 42-43 0.1305896019144015 1109 44-45 0.09289741022696063 1109 46-47 0.0986066467324207 1109 48-49 0.10195225709081512 1109 50-51 0.056412532602998056 1109 52-53 0.021816943093227792 1109 54-55 0.028190958741276972 1109 56-57 0.1943993639590289 1109 58-59 0.15854847588106935 1109 60-61 0.061262714095100534 1109 62-63 -0.28309163197256737 1109 64-65 -0.02201712028528391 1109 66-67 0.006547848178044546 1109 68-69 -0.2991241720767661 1109 70-71 0.22435053185419207 1109 72-73 -0.2599304543349774 1109 74-75 0.0036032321161769687 1109 76 0.23700209994647992 1110 1 0.0792903797108977 1110 2 -0.03520532446340496 1110 3 0.10903213886752638 1110 4 0.12827105252898718 1110 5 0.17030966963581307 1110 6 -0.0337239930028872 1110 7 0.07182859172617384 1110 8 0.07130354593277843 1110 9 0.1250693979960502 1110 10-11 -0.0889729155874619 1110 12-13 0.02128088558207253 1110 14-15 -0.003895931496550986 1110 16-17 0.09986524155762311 1110 18-19 0.12819324463757198 1110 20-21 -0.05205874313706005 1110 22-23 -0.1474924120763177 1110 24-25 0.058382589396067885 1110 26-27 0.09275880330390862 1110 28-29 0.06619496345709308 1110 30-31 0.051760545089791776 1110 32-33 0.12005226443709915 1110 34-35 0.010560680008516954 1110 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0.01653956939912149 2115 60-61 0.08617131269865297 2115 62-63 -0.07427723865792757 2115 64-65 0.10819894436917821 2115 66-67 0.04828323769918086 2115 68-69 0.12596104426278032 2115 70-71 -0.02753591050107218 2115 72-73 -0.04689304161300356 2115 74-75 -0.34812033707399337 2115 76 -0.4808218595895042 2116 1 -0.2194408698321837 2116 2 -0.18592676428582422 2116 3 -0.19653800129982812 2116 4 -0.2226391380161985 2116 5 -0.19918980864123625 2116 6 -0.03809716115718942 2116 7 -0.01219206451489896 2116 8 0.1806261036379908 2116 9 0.002075705194499733 2116 10-11 0.09629190034735302 2116 12-13 0.07964739429185386 2116 14-15 0.02773642994778669 2116 16-17 -0.40275998856539985 2116 18-19 -0.12574626208500916 2116 20-21 -0.05652163434619695 2116 22-23 -7.653097322233293E-4 2116 24-25 -0.05816765883771069 2116 26-27 0.004939871074725488 2116 28-29 -0.054718483702664855 2116 30-31 0.14666759865113477 2116 32-33 0.011015491276921807 2116 34-35 -0.010673776303001148 2116 36-37 0.0026445226763982532 2116 38-39 0.09456827226215125 2116 40-41 -0.2785634113840203 2116 42-43 -0.05984081131776264 2116 44-45 -0.06631730550122938 2116 46-47 -0.10641745449957085 2116 48-49 -0.20295389468899572 2116 50-51 -0.16876183486748886 2116 52-53 -0.1970508785235694 2116 54-55 -0.12705233757092316 2116 56-57 -0.27604168517748917 2116 58-59 -0.03050472409439209 2116 60-61 -0.15031678588103148 2116 62-63 0.01640793509157845 2116 64-65 -0.2135043060659001 2116 66-67 -0.35216166209310273 2116 68-69 -0.34394155496250534 2116 70-71 0.04440943840140932 2116 72-73 -0.18813469097020885 2116 74-75 -0.26228415001959604 2116 76 0.45930613681761656 2117 1 -0.00680337540049436 2117 2 -0.04667445986201102 2117 3 -0.05167969338083367 2117 4 0.06767371535734412 2117 5 0.029114341034940594 2117 6 0.11838435205255848 2117 7 0.04525919135324585 2117 8 -0.6595479703091698 2117 9 -0.0301074151152676 2117 10-11 0.05555585538967023 2117 12-13 0.021490248008056767 2117 14-15 0.17920132389289734 2117 16-17 -0.11138144626337265 2117 18-19 -0.009024197869543116 2117 20-21 -0.144165125419768 2117 22-23 0.10334667327158087 2117 24-25 -0.24797679429328667 2117 26-27 0.039021356871245416 2117 28-29 -0.6114261559776253 2117 30-31 0.0439159966910907 2117 32-33 0.0870220704497271 2117 34-35 0.025196422100179916 2117 36-37 0.05249203664001101 2117 38-39 0.06641146864360081 2117 40-41 -0.04653106378439276 2117 42-43 -0.03900333744598328 2117 44-45 0.14689586655099873 2117 46-47 -0.08269473922012338 2117 48-49 -0.029904516626430677 2117 50-51 0.08216507463460943 2117 52-53 -0.07230702079817775 2117 54-55 -0.011313722623867761 2117 56-57 0.03381679407412719 2117 58-59 0.062021746796197874 2117 60-61 -0.013535097912011906 2117 62-63 0.18055144329841255 2117 64-65 0.1464415528245553 2117 66-67 0.28255375441651154 2117 68-69 0.0356253161610951 2117 70-71 0.28363775625769705 2117 72-73 -0.3951833151692341 2117 74-75 0.35472575300614295 2117 76 -0.0991971013690005 2118 1 0.047742079144960314 2118 2 -0.025895239082792898 2118 3 -0.08284852454966085 2118 4 0.046894494578126 2118 5 -0.10854799662739367 2118 6 -0.1309662972980874 2118 7 0.04525919135324585 2118 8 -0.15045706121826186 2118 9 -0.08725027225812454 2118 10-11 -0.03405453422071503 2118 12-13 0.09941232593013893 2118 14-15 0.11946106415263813 2118 16-17 0.0691380342561061 2118 18-19 -0.11811510696045247 2118 20-21 0.02336734211269942 2118 22-23 0.051398621323535565 2118 24-25 0.10786736155086629 2118 26-27 -0.12331630546641748 2118 28-29 -0.043893688445159285 2118 30-31 -0.18335673058163593 2118 32-33 -0.13765325422559727 2118 34-35 -0.10337500647124642 2118 36-37 0.012232296380268792 2118 38-39 0.23394393617606823 2118 40-41 0.1067156894623622 2118 42-43 0.02593172748908046 2118 44-45 0.030012749667875482 2118 46-47 0.060162403637015416 2118 48-49 0.009056522334617512 2118 50-51 -0.02302973056018942 2118 52-53 0.05886181037065796 2118 54-55 0.11076419945405291 2118 56-57 0.08057004082738217 2118 58-59 -0.01200422722977379 2118 60-61 0.08256879819188612 2118 62-63 3.196277893380284E-5 2118 64-65 -0.29122078483779035 2118 66-67 -0.18368001181725901 2118 68-69 -0.5150240344882562 2118 70-71 -0.428050555430616 2118 72-73 -0.3198586398445613 2118 74-75 -0.0037158054354193837 2118 76 0.11378991161801366 2119 1 -0.06329688189399718 2119 2 -0.03628484947240196 2119 3 -0.08644492814606508 2119 4 -0.04111749343386606 2119 5 0.09959386151446381 2119 6 -0.19774951408130903 2119 7 -0.2451503990563495 2119 8 -0.11424327500446907 2119 9 -0.28095656596442353 2119 10-11 -0.09132226648844721 2119 12-13 -0.2157475142297045 2119 14-15 -0.6859585162669433 2119 16-17 -0.14507275495467553 2119 18-19 -0.03946875331410382 2119 20-21 -0.019140150394790112 2119 22-23 0.014460559385469196 2119 24-25 -0.07137839269488921 2119 26-27 0.11262275547264267 2119 28-29 0.03410331455184945 2119 30-31 -0.09536971759462887 2119 32-33 -0.2744414660138119 2119 34-35 -0.024478902575147288 2119 36-37 0.014380148528118752 2119 38-39 -0.1475995203673861 2119 40-41 -0.1022753195286441 2119 42-43 -0.2996926481352986 2119 44-45 -0.10262960797447818 2119 46-47 -0.05170073322612012 2119 48-49 -0.08979462651653591 2119 50-51 -0.09463312716358985 2119 52-53 0.014306365815208721 2119 54-55 -0.22567436198450253 2119 56-57 -0.18878560352825957 2119 58-59 -0.12406716429271825 2119 60-61 -0.15966396904087787 2119 62-63 -0.15388911614214607 2119 64-65 -0.13575125436825886 2119 66-67 0.04986144672420778 2119 68-69 -0.3716424161066385 2119 70-71 -0.09365994604000605 2119 72-73 -0.14683166681758308 2119 74-75 -0.10533918205879189 2119 76 -1.4265197786916772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 3.0 21 2.0 22 5.0 23 16.0 24 32.0 25 53.0 26 141.0 27 284.0 28 459.0 29 837.0 30 1329.0 31 2004.0 32 3145.0 33 5134.0 34 8639.0 35 14585.0 36 22121.0 37 88655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.311539896232496 15.234155108684593 11.68978263081149 35.76452236427142 2 20.11936654345688 16.729627996880193 36.402048221370684 26.748957238292242 3 19.97083658313269 16.14703787853098 31.052256773712234 32.829868764624095 4 16.442174656140637 21.86253764140962 29.317571416944737 32.377716285505 5 17.61877310183458 28.118959612058735 36.948014513886534 17.31425277222015 6 32.16724880463902 35.01848146766591 14.516599409949475 18.297670317745602 7 29.789413001458172 22.38800908813456 19.658855844552207 28.16372206585506 8 25.26704873003493 35.5135813354132 14.699040320119366 24.5203296144325 9 23.013327003289362 14.29482179795856 15.27823934348401 47.413611855268066 10-11 28.58048763945878 24.40435416596019 21.65587168096578 25.35928651361525 12-13 26.595001525992746 16.636372884804505 24.23073010275018 32.53789548645258 14-15 23.881786428837874 16.2755603784462 18.910102071959038 40.93255112075689 16-17 30.150225507816476 22.707111126182646 17.102987554681405 30.039675811319476 18-19 32.49754145613618 20.872867849028452 17.41327274576961 29.21631794906575 20-21 26.706568550985114 19.005391841025467 17.533656617721864 36.75438299026756 22-23 25.74010648038251 23.6210112245244 17.349520160059683 33.2893621350334 24-25 30.444572552477194 23.65899148835159 17.842924480314693 28.053511478856525 26-27 31.522940757570623 25.24839770761979 17.973142527722203 25.25551900708739 28-29 26.304723795313507 24.477262708128457 19.904032011936653 29.313981484621383 30-31 24.826884601037673 25.573264607141645 22.863779714469803 26.736071077350875 32-33 27.61402556885618 25.874732951269962 20.921699616806265 25.589541863067584 34-35 27.05754688188816 19.31092949913527 28.494692936349146 25.13683068262742 36-37 25.050018651022416 26.02801044457255 23.637627589948796 25.284343314456237 38-39 23.102851910882023 22.590796568211875 25.650581572789854 28.655769948116244 40-41 24.048289192580285 17.30170572077724 27.065007290854215 31.58499779578826 42-43 28.36108379395707 17.047034487435994 26.355929329580523 28.235952389026416 44-45 22.711180440164128 22.548746990403203 29.078639492692187 25.66143307674048 46-47 26.290481196378312 19.523551154667842 26.177218623893655 28.008749025060194 48-49 25.974770253314794 18.26545491539218 22.716945301637896 33.04282952965513 50-51 23.247312557224728 19.072196412221505 26.548204415205667 31.132286615348097 52-53 19.690053918410257 18.865339618162704 33.97639797890739 27.46820848451965 54-55 20.356404082878363 19.509308555732645 29.646647902607754 30.487639458781242 56-57 24.400284851978704 20.435416596018854 23.94655634304317 31.21774220895927 58-59 21.76540404896741 20.8630336735732 29.158330224829598 28.213232052629795 60-61 24.926921903082505 24.6766590932212 23.95537319000305 26.441045813693243 62-63 20.62803079114246 32.689477432262876 19.98507918206789 26.69741259452677 64-65 19.486249109837566 34.920817932110275 18.97249821967513 26.620434738377025 66-67 19.997287124012345 34.46234189019635 18.941978364814 26.59839262097731 68-69 19.486588219336024 35.66550239072196 19.05795381328631 25.789955576655704 70-71 19.475058496388485 34.63562684390789 19.66292515853369 26.226389501169926 72-73 19.16714707178948 32.41818983349724 20.788429583912645 27.626233510800635 74-75 19.271592797314252 31.601953270711114 21.662653870934925 27.463800061039713 76 19.70294007935162 31.59211909525586 22.24626131777951 26.45867950761301 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 3.0 19 8.0 20 19.0 21 26.0 22 28.0 23 59.5 24 85.5 25 82.0 26 128.0 27 207.5 28 317.5 29 394.0 30 475.5 31 611.5 32 829.0 33 992.0 34 1108.0 35 1538.0 36 4279.0 37 6706.0 38 8125.0 39 8967.0 40 7490.0 41 5963.0 42 5336.0 43 5069.5 44 4801.0 45 4726.0 46 4653.0 47 4678.5 48 4457.0 49 4065.5 50 3921.0 51 3630.5 52 3120.5 53 2895.0 54 2889.0 55 3066.0 56 3244.5 57 3381.5 58 3517.0 59 3579.5 60 3941.5 61 4284.5 62 4328.0 63 4475.0 64 4768.5 65 5122.0 66 5377.5 67 5426.0 68 5367.5 69 5205.0 70 4808.0 71 4515.0 72 4146.0 73 3383.5 74 2502.0 75 2014.0 76 1630.0 77 911.0 78 391.0 79 206.0 80 128.0 81 30.0 82 8.5 83 7.0 84 4.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 6.782189969141035E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.88392281867815 #Duplication Level Percentage of deduplicated Percentage of total 1 62.1571577363731 29.1417138594052 2 21.931778729313738 20.564956424429447 3 7.909964124522624 11.125504425378955 4 3.0899201481310037 5.794703109634101 5 1.395961115611619 3.2724066601105495 6 0.8722948732785557 2.453796330835227 7 0.6263742622381668 2.0556817796466476 8 0.4629093854877908 1.7362406321001052 9 0.305230876056012 1.2879378751398827 >10 1.2180303205647494 8.234935060531045 >50 0.013019326466844115 0.45372850893553535 >100 0.010126142807545422 0.9196649598155243 >500 0.0014465918296493463 0.4225304350774865 >1k 0.004339775488948039 5.391841025467124 >5k 0.0 0.0 >10k+ 0.0014465918296493463 7.144358913493167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 10534 7.144358913493167 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 3053 2.070602597578758 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 2632 1.7850723998779203 No Hit CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG 2265 1.5361660280104446 Illumina PCR Primer Index 8 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 623 0.4225304350774865 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 372 0.25229746685204657 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 258 0.1749805012038387 No Hit CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 176 0.11936654345688223 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 170 0.11529722947539761 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004747532978398725 0.0 2 0.0 0.0 0.0 0.008138627962969243 0.0 3 0.0 0.0 0.0 0.008138627962969243 0.0 4 0.0 0.0 0.0 0.042049577808674424 0.0 5 0.0 0.0 0.0 0.045440672793244936 0.0 6 0.0 6.782189969141035E-4 0.0 0.04747532978398725 0.0 7 0.0 6.782189969141035E-4 0.0 0.04747532978398725 0.0 8 0.0 6.782189969141035E-4 0.0 0.050866424768557766 0.0 9 0.0 6.782189969141035E-4 0.0 0.05832683373461291 0.0 10 0.0 6.782189969141035E-4 0.0 0.06578724270066805 0.0 11 0.0 6.782189969141035E-4 0.0 0.06985655668215267 0.0 12 0.0 6.782189969141035E-4 0.0 0.07324765166672319 0.0 13 0.0 6.782189969141035E-4 0.0 0.0759605276543796 0.0 14 0.0 6.782189969141035E-4 0.0 0.07799518464512191 0.0 15 0.0 6.782189969141035E-4 0.0 0.07867340364203601 0.0 16 0.0 6.782189969141035E-4 0.0 0.07935162263895011 0.0 17 0.0 6.782189969141035E-4 0.0 0.08274271762352063 0.0 18 0.0 6.782189969141035E-4 0.0 0.08409915561734885 0.0 19 0.0 6.782189969141035E-4 0.0 0.09088134558648987 0.0 20 0.0 6.782189969141035E-4 0.0 0.09766353555563091 0.0 21 0.0 6.782189969141035E-4 0.0 0.10241106853402963 0.0 22 0.0 6.782189969141035E-4 0.0 0.10783682050934247 0.0 23 0.0 6.782189969141035E-4 0.0 0.1139407914815694 0.0 24 0.0 6.782189969141035E-4 0.0 0.11597544847231171 0.0 25 0.0 6.782189969141035E-4 0.0 0.12547051442910917 0.0 26 0.0 6.782189969141035E-4 0.0 0.14785174132727458 0.0 27 0.0 6.782189969141035E-4 0.0 0.3004510156329479 0.0 28 0.0 6.782189969141035E-4 0.0 0.4408423479941673 0.0 29 0.0 6.782189969141035E-4 0.0 0.6002238122689817 0.0 30 0.0 6.782189969141035E-4 0.0 0.7616399335345383 0.0 31 0.0 6.782189969141035E-4 0.0 0.9488283766828309 0.0 32 0.0 6.782189969141035E-4 0.0 1.184848587608939 0.0 33 0.0 6.782189969141035E-4 0.0 1.4744480992912612 0.0 34 0.0 6.782189969141035E-4 0.0 1.9017260673471463 0.0 35 0.0 6.782189969141035E-4 0.0 2.4972023466377293 0.0 36 0.0 6.782189969141035E-4 0.0 3.285971040048832 0.0 37 0.0 6.782189969141035E-4 0.0 4.321611448336668 0.0 38 0.0 6.782189969141035E-4 0.0 5.399301434433179 0.0 39 0.0 6.782189969141035E-4 0.0 6.49530333344637 0.0 40 0.0 6.782189969141035E-4 0.0 7.447522805113771 0.0 41 0.0 6.782189969141035E-4 0.0 8.429583912645393 0.0 42 0.0 6.782189969141035E-4 0.0 9.410288582183187 0.0 43 0.0 6.782189969141035E-4 0.0 10.32995354199871 0.0 44 0.0 6.782189969141035E-4 0.0 11.250974939808064 0.0 45 0.0 6.782189969141035E-4 0.0 12.273050968157618 0.0 46 0.0 6.782189969141035E-4 0.0 13.308691376445454 0.0 47 0.0 6.782189969141035E-4 0.0 14.26158906710977 0.0 48 0.0 6.782189969141035E-4 0.0 15.078164739394351 0.0 49 0.0 6.782189969141035E-4 0.0 15.905591915629557 0.0 50 0.0 6.782189969141035E-4 0.0 16.690291295059176 0.0 51 0.0 6.782189969141035E-4 0.0 17.42819356370172 0.0 52 0.0 6.782189969141035E-4 0.0 18.181016650276373 0.0 53 0.0 6.782189969141035E-4 0.0 18.901285224999153 0.0 54 0.0 6.782189969141035E-4 0.0 19.625623113703416 0.0 55 0.0 6.782189969141035E-4 0.0 20.38048085726881 0.0 56 0.0 6.782189969141035E-4 0.0 21.057343416189088 0.0 57 0.0 6.782189969141035E-4 0.0 21.719285157177254 0.0 58 0.0 6.782189969141035E-4 0.0 22.400217030079013 0.0 59 0.0 6.782189969141035E-4 0.0 23.03638644918444 0.0 60 0.0 6.782189969141035E-4 0.0 23.745803519956596 0.0 61 0.0 6.782189969141035E-4 0.0 24.413849231916988 0.0 62 0.0 6.782189969141035E-4 0.0 25.077825629895894 0.0 63 0.0 6.782189969141035E-4 0.0 25.752653531825427 0.0 64 0.0 6.782189969141035E-4 0.0 26.387466512937028 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATTA 15 0.0022210479 70.00001 57 CATTAGA 15 0.0022210479 70.00001 2 TGAAATT 25 2.3841819E-4 55.999996 48 TCTTGTC 20 0.0069400812 52.5 22 GCCTTAT 20 0.0069400812 52.5 12 GATGTTA 20 0.0069400812 52.5 53 CAAGTAG 20 0.0069400812 52.5 31 CCAGGTA 20 0.0069400812 52.5 3 ACATTAG 20 0.0069400812 52.5 1 TGCTCAA 20 0.0069400812 52.5 20 ATTTCGG 20 0.0069400812 52.5 48 TCTTTAT 90 0.0 50.555553 1 TATGAAT 30 5.862646E-4 46.666668 2 GTATGAA 30 5.862646E-4 46.666668 1 AAACTCG 45 1.6757349E-6 46.666664 36 AACTCGT 45 1.6757349E-6 46.666664 37 CTTTATA 110 0.0 44.545456 2 TAATACA 60 2.5792906E-7 40.833336 4 TTAATAC 35 0.0012522114 40.0 3 ATGGCTC 35 0.0012522114 40.0 7 >>END_MODULE