FastQCFastQC Report
Wed 25 May 2016
SRR1780175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606744
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAC300.00574067929.33553526
GAGTAAC300.005759216429.316191
AGTAACG300.005759216429.316192
TCCTATA454.0824925E-529.3161892
CTTAATG407.035151E-427.49525812
TCCAACG407.052124E-427.4839252
TTCATTC450.001393447624.45636235
ATCTTAA450.001400160524.436210
GGACTAT450.001403526824.4261286
ATGGCAC551.5826467E-424.02161216
CTATACC651.8219971E-523.6745594
TCATTGA500.00257784622.00164825
TTCTACA703.226476E-521.987142
ACCGATC602.8850793E-421.9835178
CAAGGAA602.8850793E-421.9835174
CTTCATT654.912088E-420.31759534
CATTGAA550.00447913320.00150126
ATACCTC550.00447913320.00150129
AAAGATA550.00447913320.00150125
CAAGTAG550.004489835419.99325631
Produced by FastQC (version 0.11.5)