FastQCFastQC Report
Wed 25 May 2016
SRR1780175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606744
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT196783.2432129530741136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC60270.9933349155492268No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG49370.8136874859907969No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG36080.5946494732539588Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC10560.17404374826945138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1100.042.003622
TCTTTAT1300.035.6149141
TCTTCTG5550.033.29742444
CCGTCTT5550.033.29742441
TTACTTC401.819565E-532.99740629
GTCTTCT5700.032.42117743
ATGCCGT29550.031.79030444
TTAATAC353.2143632E-431.4312783
CGGCTTA353.2174724E-431.426125
CGTCTTC6100.030.65585342
GTTAATA454.063851E-529.3358612
TAGCGAG300.005744995529.33102428
TATGCCG33550.027.86895843
ACAGTAG407.0313E-427.49783711
ACACACG407.0313E-427.49783730
TTATCAA407.0313E-427.49783732
TATAGAG407.0313E-427.4978377
AATAGAC407.0313E-427.49783719
CTTAATA655.918555E-727.0792562
TTTATAC1900.026.6338733