FastQCFastQC Report
Wed 25 May 2016
SRR1780174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284932
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGT207.9184014E-570.023
CAGAGTA207.9184014E-570.024
ATTCGCG200.006946698352.562
TCTAACG200.006946698352.511
GTTATTG200.006946698352.534
TAGTAAA200.006946698352.533
AATTATC350.001254179740.030
CATTTCC459.184484E-538.88888549
TTCCGTT501.707262E-435.00000421
AATTTCA400.002416390735.016
AGAGTAC400.002416390735.025
GAAAGCA400.002416390735.033
AGATTGC400.002416390735.027
CTGCACT552.9878467E-431.818184
AGTGCGA450.004303077231.11110910
CAATATA450.004303077231.11110936
TAACTCT450.004303077231.11110913
ATTTCCG450.004303077231.11110950
TCTTAGG604.975E-429.1666662
GAGTCTC500.007201411328.00000235