##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284932 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.087943790097288 34.0 32.0 34.0 21.0 34.0 2 31.10679039209355 34.0 33.0 34.0 21.0 34.0 3 30.71220852694678 34.0 32.0 34.0 21.0 34.0 4 30.528936728763355 34.0 32.0 34.0 21.0 34.0 5 30.664607695871297 34.0 32.0 34.0 21.0 34.0 6 33.816882624626224 37.0 34.0 38.0 22.0 38.0 7 34.293505117010376 38.0 34.0 38.0 24.0 38.0 8 34.40383670489801 38.0 35.0 38.0 25.0 38.0 9 34.667573315738494 38.0 36.0 38.0 25.0 38.0 10-11 34.333739278143554 38.0 34.5 38.0 23.5 38.0 12-13 34.43691828225682 38.0 35.0 38.0 24.5 38.0 14-15 34.40879929246276 38.0 35.0 38.0 24.5 38.0 16-17 34.532414751589855 38.0 35.0 38.0 25.0 38.0 18-19 34.53446611823172 38.0 35.0 38.0 25.0 38.0 20-21 34.578281835666054 38.0 35.0 38.0 25.0 38.0 22-23 34.62393658837898 38.0 35.5 38.0 25.0 38.0 24-25 34.588278957786414 38.0 35.0 38.0 25.0 38.0 26-27 34.5657806073028 38.0 35.0 38.0 25.0 38.0 28-29 34.68134677747673 38.0 36.0 38.0 25.0 38.0 30-31 34.68307876967136 38.0 36.0 38.0 25.0 38.0 32-33 34.780456389594704 38.0 36.0 38.0 25.5 38.0 34-35 34.69445341344601 38.0 36.0 38.0 25.0 38.0 36-37 34.59733550461162 38.0 35.0 38.0 25.0 38.0 38-39 34.596635337554225 38.0 35.5 38.0 25.0 38.0 40-41 34.686425182148724 38.0 35.5 38.0 25.5 38.0 42-43 34.7282246290343 38.0 36.0 38.0 25.0 38.0 44-45 34.84235712380497 38.0 36.0 38.0 26.0 38.0 46-47 34.831247455533244 38.0 36.0 38.0 26.0 38.0 48-49 34.81339056336249 38.0 36.0 38.0 26.0 38.0 50-51 34.73478057922592 38.0 36.0 38.0 25.5 38.0 52-53 34.78818805890528 38.0 36.0 38.0 25.5 38.0 54-55 34.763734856035825 38.0 36.0 38.0 25.5 38.0 56-57 34.73598788482866 38.0 36.0 38.0 25.5 38.0 58-59 34.81833209327138 38.0 36.0 38.0 26.0 38.0 60-61 34.82580054188368 38.0 36.0 38.0 26.0 38.0 62-63 34.66513062765853 38.0 36.0 38.0 25.0 38.0 64-65 34.64182155742422 38.0 36.0 38.0 25.0 38.0 66-67 34.469492721070296 38.0 35.0 38.0 24.5 38.0 68-69 34.39018432468097 38.0 35.0 38.0 24.5 38.0 70-71 34.32639015624781 38.0 35.0 38.0 24.0 38.0 72-73 34.34214654724636 38.0 35.0 38.0 24.0 38.0 74-75 34.33643992250782 38.0 35.0 38.0 24.0 38.0 76 32.05780677494981 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08123555099500734 1101 2 0.754500315906899 1101 3 0.5285195433126866 1101 4 0.7529353624043225 1101 5 0.1652603118813829 1101 6 0.23224266883335076 1101 7 -0.040417247860993655 1101 8 -0.29895015821598747 1101 9 0.1384405698160549 1101 10-11 -0.05812352409405719 1101 12-13 0.5608916297948454 1101 14-15 0.07639703606420767 1101 16-17 0.03343338566197218 1101 18-19 0.29527029813989714 1101 20-21 0.48744602548696037 1101 22-23 0.05877238883492453 1101 24-25 0.0206070942352099 1101 26-27 -0.231587278395871 1101 28-29 0.006342828223473873 1101 30-31 0.14376086680704248 1101 32-33 0.21686221905160608 1101 34-35 0.41263609889850983 1101 36-37 0.2156305759165562 1101 38-39 0.6454733511511179 1101 40-41 0.6764061571096889 1101 42-43 0.09318434668940512 1101 44-45 0.5397046960085135 1101 46-47 0.5986048382096598 1101 48-49 0.4651802555063682 1101 50-51 0.2716252477497463 1101 52-53 0.5698045506236156 1101 54-55 0.45534272375136453 1101 56-57 0.953630517872746 1101 58-59 0.3263619596616323 1101 60-61 0.44669102463089416 1101 62-63 0.24889869957257815 1101 64-65 0.6942827239211837 1101 66-67 0.26587738367185665 1101 68-69 0.3337561632892303 1101 70-71 0.6304311559307294 1101 72-73 0.34980970093171493 1101 74-75 0.14335628439277315 1101 76 -1.171506078328079 1102 1 0.12466110534921526 1102 2 0.02266420743653441 1102 3 0.3238198511034618 1102 4 0.33847829466838775 1102 5 -0.14248183412000515 1102 6 -0.02059286547072503 1102 7 -0.7075090485782312 1102 8 -0.38299806188155827 1102 9 0.02197737136473421 1102 10-11 0.19300676772674308 1102 12-13 0.3251249923071242 1102 14-15 0.08517425013410218 1102 16-17 0.1283535828237703 1102 18-19 0.06868247736763067 1102 20-21 0.07126018386273358 1102 22-23 -0.09036181233663143 1102 24-25 0.026401310529607258 1102 26-27 -0.1503168649020168 1102 28-29 -0.02808989235207804 1102 30-31 0.3404583125514691 1102 32-33 0.1357337783680066 1102 34-35 0.21591669050940965 1102 36-37 0.2179860695583713 1102 38-39 0.12491553135148337 1102 40-41 -0.01653253887398165 1102 42-43 -0.06334891259844255 1102 44-45 0.0373609680691942 1102 46-47 -0.3181253632505445 1102 48-49 -0.2632288960139135 1102 50-51 0.2893341987824627 1102 52-53 0.22717082164892588 1102 54-55 -0.0018983601992701438 1102 56-57 0.18804096205154508 1102 58-59 0.04483458322504674 1102 60-61 0.3506232792846191 1102 62-63 0.029262055841194012 1102 64-65 -0.012999147798147703 1102 66-67 0.22219682092615045 1102 68-69 0.3178779555665372 1102 70-71 -0.05849935739902179 1102 72-73 0.21066850308908158 1102 74-75 -0.12487035755022902 1102 76 0.04778465374961627 1103 1 0.5773184844790897 1103 2 0.5701183752211811 1103 3 0.5545651955271715 1103 4 1.0694150777156715 1103 5 0.8726674427846604 1103 6 -0.1251454869739561 1103 7 0.4538435632777009 1103 8 0.3810395143547325 1103 9 0.6712301577765061 1103 10-11 0.32064583205917074 1103 12-13 0.9859959928226445 1103 14-15 0.41381934704425305 1103 16-17 0.7411531390479382 1103 18-19 0.4887711722385788 1103 20-21 0.6934922376223156 1103 22-23 0.6943307854704202 1103 24-25 0.7585470209477876 1103 26-27 0.31086405514898985 1103 28-29 0.6558245903566515 1103 30-31 0.3807629260715615 1103 32-33 0.6239309088989984 1103 34-35 0.2241636252303465 1103 36-37 0.44756846202865574 1103 38-39 0.21004466951590217 1103 40-41 0.5889418520647993 1103 42-43 0.575612163557949 1103 44-45 0.7269748951536954 1103 46-47 0.5515885942832455 1103 48-49 0.6090348129511369 1103 50-51 -0.10257608735044954 1103 52-53 0.8067957225379843 1103 54-55 0.7521241119818356 1103 56-57 0.5041400458594509 1103 58-59 0.25647450375540615 1103 60-61 0.5872949303899873 1103 62-63 0.35479062240304415 1103 64-65 0.6780294654928198 1103 66-67 0.6268505883478142 1103 68-69 0.7449262734692113 1103 70-71 0.6643125895770012 1103 72-73 0.6091743773907723 1103 74-75 0.402475217805744 1103 76 0.8607204780963897 1104 1 0.3646045480487743 1104 2 0.14835798402313927 1104 3 0.03867277498927635 1104 4 0.6060898943415864 1104 5 -0.5405354912006715 1104 6 -0.19116015690060806 1104 7 -0.20752562987632928 1104 8 -0.08473065679441305 1104 9 -0.11361091801811085 1104 10-11 -0.016762480899267018 1104 12-13 -0.13136342038028204 1104 14-15 -0.12346671652542796 1104 16-17 0.014380522911018545 1104 18-19 0.1519741062239106 1104 20-21 -0.46054199220652947 1104 22-23 -0.20970344435843202 1104 24-25 0.07761792559327318 1104 26-27 0.03396918962331341 1104 28-29 -0.11067907712502034 1104 30-31 -0.005545142388100999 1104 32-33 -0.015433394279483537 1104 34-35 0.05240323403229752 1104 36-37 -0.17468092672439894 1104 38-39 -0.07112892461612574 1104 40-41 -0.32915105747065354 1104 42-43 0.062164730795110756 1104 44-45 -0.048086229540679426 1104 46-47 0.18117294636147818 1104 48-49 -0.29316567604641364 1104 50-51 -0.14964210202037975 1104 52-53 -0.04161503540822764 1104 54-55 -0.06511305183234839 1104 56-57 0.01575373779099465 1104 58-59 -0.1127186502788291 1104 60-61 0.0885174242775193 1104 62-63 0.05894710162064598 1104 64-65 0.055780076739772255 1104 66-67 0.24848873015711348 1104 68-69 -0.03808106149411827 1104 70-71 0.2835057436112294 1104 72-73 0.3567293896157153 1104 74-75 0.029003335165157296 1104 76 0.591771822839668 1105 1 -0.011251501775454642 1105 2 0.38110014120787383 1105 3 0.2992555578962559 1105 4 0.4181968412695376 1105 5 0.5249409164919179 1105 6 0.6081683837701206 1105 7 0.17559239856337427 1105 8 -0.41407111830105947 1105 9 0.4422649593189192 1105 10-11 0.7826724958064446 1105 12-13 -0.5574527171726018 1105 14-15 0.36834379083994406 1105 16-17 0.22254507910316335 1105 18-19 0.3210051733820123 1105 20-21 0.4655564276744997 1105 22-23 0.29536139039202425 1105 24-25 0.06011375938275876 1105 26-27 0.35808364181225016 1105 28-29 0.2599175120562194 1105 30-31 0.2994518774029231 1105 32-33 0.09861281720587556 1105 34-35 0.3207102320495494 1105 36-37 -0.05850447402457348 1105 38-39 0.39526921913942203 1105 40-41 0.03660421263359126 1105 42-43 0.21069317658572118 1105 44-45 0.36123635071209037 1105 46-47 0.49415570980587376 1105 48-49 0.246084892626115 1105 50-51 0.4075961565644519 1105 52-53 0.21892061154039766 1105 54-55 0.08293947283039671 1105 56-57 0.04678592404797399 1105 58-59 0.14232033572027802 1105 60-61 0.3965220223142296 1105 62-63 0.4592591112546316 1105 64-65 -0.0711601001265123 1105 66-67 0.6152232117832099 1105 68-69 0.3753088958098303 1105 70-71 0.46605266669750733 1105 72-73 0.4395518299811272 1105 74-75 0.466710885433379 1105 76 0.7965638772376025 1106 1 -0.00430096036486205 1106 2 -0.08131206247956868 1106 3 0.3468850574572748 1106 4 0.38829122230553637 1106 5 -0.01573804165850845 1106 6 0.5064344065971582 1106 7 -0.7183752631281664 1106 8 -0.05458180018575831 1106 9 -0.027664803631040513 1106 10-11 -0.3228294064469921 1106 12-13 0.14637190969306602 1106 14-15 0.17326221343286363 1106 16-17 0.28659066002209244 1106 18-19 -0.050336646928208495 1106 20-21 0.3393675192753207 1106 22-23 -0.041886195209379196 1106 24-25 0.1767822551392726 1106 26-27 0.01411415103086 1106 28-29 0.1745904707919479 1106 30-31 -0.1747122285379632 1106 32-33 0.13909972092258727 1106 34-35 0.2602496672266028 1106 36-37 0.17701827985309393 1106 38-39 0.15882277489007635 1106 40-41 0.5989944672428962 1106 42-43 0.13725521931326057 1106 44-45 0.2639927891083431 1106 46-47 0.24693592637011363 1106 48-49 0.3602932625412478 1106 50-51 0.3323291094470946 1106 52-53 0.13187422582193165 1106 54-55 -0.049175686666238505 1106 56-57 -0.11091820237871985 1106 58-59 0.1765060249213306 1106 60-61 0.2329739538331097 1106 62-63 0.06697421222038713 1106 64-65 0.28003429554220105 1106 66-67 -0.21109556199495927 1106 68-69 0.3445984773140722 1106 70-71 0.18034883292519766 1106 72-73 0.1734466938968353 1106 74-75 -0.0490924508264996 1106 76 0.11429507419399698 1107 1 0.10612215625020482 1107 2 -0.39814274211984824 1107 3 -0.06406879776902485 1107 4 -0.6323025415157844 1107 5 0.12907251414275933 1107 6 -0.8999676279008497 1107 7 0.18153075190942758 1107 8 5.007591537662393E-4 1107 9 -0.016718319774334134 1107 10-11 -0.21288542885595518 1107 12-13 0.2512434635777794 1107 14-15 0.09057848204557217 1107 16-17 0.2903638798363417 1107 18-19 0.25365863539524725 1107 20-21 0.4257507567808432 1107 22-23 0.2149691785190626 1107 24-25 0.2507769846100061 1107 26-27 -0.1101625333270917 1107 28-29 0.1635057737552401 1107 30-31 0.13555332505189455 1107 32-33 0.3442034729497152 1107 34-35 0.2936650070467408 1107 36-37 0.4028447946695479 1107 38-39 0.1449185289811865 1107 40-41 0.40539465889850845 1107 42-43 0.42461884897565483 1107 44-45 0.280942332083427 1107 46-47 0.2145387569760402 1107 48-49 0.45586306941136456 1107 50-51 0.21424898265954084 1107 52-53 0.15408969095535952 1107 54-55 0.11351965462817759 1107 56-57 0.2336196127473329 1107 58-59 0.17684695076534496 1107 60-61 0.32945136058528135 1107 62-63 0.6266178065002066 1107 64-65 0.6527906349200592 1107 66-67 0.17429346203569906 1107 68-69 0.4765367789505106 1107 70-71 0.4561117696788415 1107 72-73 0.37980357875604653 1107 74-75 0.46351259119030175 1107 76 0.07521760109949582 1108 1 -0.16757070722784917 1108 2 -0.03405589120711383 1108 3 -0.14597053114068004 1108 4 0.22997027987184282 1108 5 0.3248424568787627 1108 6 0.45536274488685535 1108 7 0.2037116665882337 1108 8 0.26681698127504205 1108 9 -0.14448378769815662 1108 10-11 0.5542891351743435 1108 12-13 0.30971192202351716 1108 14-15 0.5432506989627086 1108 16-17 0.19678761488295748 1108 18-19 0.43304745887194684 1108 20-21 0.09222195944843037 1108 22-23 0.20063695461774955 1108 24-25 0.09236925134342755 1108 26-27 0.2189802990930474 1108 28-29 0.16118261073054185 1108 30-31 0.2109885221692238 1108 32-33 0.01982748983533611 1108 34-35 0.1579635462430673 1108 36-37 -0.07301269739600258 1108 38-39 0.227874840845935 1108 40-41 0.2572035893627671 1108 42-43 0.2036054647135188 1108 44-45 0.18470991998042052 1108 46-47 0.318126178056481 1108 48-49 0.23615458117474475 1108 50-51 0.26035762664928797 1108 52-53 0.3030031274442351 1108 54-55 0.41487106026928444 1108 56-57 0.1078929965768225 1108 58-59 0.2334905634963107 1108 60-61 -0.12533233107296127 1108 62-63 0.2376467660754713 1108 64-65 0.368011949612459 1108 66-67 0.4817529245019827 1108 68-69 0.24882740069600118 1108 70-71 0.5450764749067716 1108 72-73 0.2519755314491263 1108 74-75 0.3023799507111775 1108 76 0.23890672526626133 1109 1 0.21606218489324291 1109 2 0.11011450814867985 1109 3 0.49979622192306294 1109 4 0.14524400341797872 1109 5 0.02832958539231356 1109 6 0.6698497727436177 1109 7 0.4295839205453049 1109 8 0.4257603366689864 1109 9 0.5423131875654406 1109 10-11 0.435073754817509 1109 12-13 -0.051332171939584725 1109 14-15 0.4469716972263811 1109 16-17 0.501751724447864 1109 18-19 0.4498634331038076 1109 20-21 0.23696405789076636 1109 22-23 0.4458589028298334 1109 24-25 -0.10400505978670083 1109 26-27 0.7037024863649961 1109 28-29 0.0018963931608979578 1109 30-31 0.58669233328677 1109 32-33 0.2877341747040987 1109 34-35 0.4455737968784703 1109 36-37 0.2714560549631386 1109 38-39 0.5103633911365719 1109 40-41 0.24512792062424893 1109 42-43 0.5885107215141403 1109 44-45 0.4106639575757285 1109 46-47 0.42130567426927 1109 48-49 0.3545159516168752 1109 50-51 0.6174556600673426 1109 52-53 0.05933270921529754 1109 54-55 0.27810522569871665 1109 56-57 0.438339000502431 1109 58-59 0.6151189700993953 1109 60-61 0.5411972431488365 1109 62-63 0.600850262640563 1109 64-65 0.6040639073869372 1109 66-67 0.5547982955480606 1109 68-69 0.5168048598670936 1109 70-71 0.6221630586154703 1109 72-73 0.24767638577358753 1109 74-75 0.42333401976173235 1109 76 0.2938669029254939 1110 1 0.2113853141449411 1110 2 0.33465298766262563 1110 3 0.5603261590887669 1110 4 0.27838065502518816 1110 5 0.18943333987120425 1110 6 0.7894945772303004 1110 7 0.04482448241891035 1110 8 0.4669531496720509 1110 9 0.5442929871188511 1110 10-11 0.3585431425726142 1110 12-13 0.4572020623648214 1110 14-15 0.41227489865963207 1110 16-17 0.4570975077993822 1110 18-19 0.14323467473023044 1110 20-21 0.36848928248441837 1110 22-23 0.3762024142802147 1110 24-25 0.4258233792224928 1110 26-27 0.4859699735125318 1110 28-29 0.28878557631507107 1110 30-31 0.12531302965691538 1110 32-33 0.3727146363544094 1110 34-35 0.16327223121403023 1110 36-37 0.24934558863920842 1110 38-39 0.22053696438266712 1110 40-41 0.2890015769570127 1110 42-43 0.4475100464349495 1110 44-45 0.36604057387110345 1110 46-47 0.19211628859133612 1110 48-49 0.5497858534707518 1110 50-51 0.335238479301907 1110 52-53 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-0.5757657740770696 2116 8 -0.21320137940430328 2116 9 -1.5132022302805268 2116 10-11 -0.662259777135418 2116 12-13 -0.8415386742485396 2116 14-15 -0.4498190446177972 2116 16-17 -0.7239977541756986 2116 18-19 -0.5837057609971907 2116 20-21 -0.626907348335962 2116 22-23 -0.34848416286359196 2116 24-25 -0.5854431277146048 2116 26-27 -0.5735692252393534 2116 28-29 -1.1294867440355816 2116 30-31 -0.6120163290102667 2116 32-33 -0.6536305281113357 2116 34-35 -0.5693794654483639 2116 36-37 -0.9336506409999217 2116 38-39 -0.5720792574557052 2116 40-41 -1.008510542060094 2116 42-43 -0.467820278038964 2116 44-45 -0.609172553671101 2116 46-47 -0.42786012343666613 2116 48-49 -0.466456415432134 2116 50-51 -0.628202950092053 2116 52-53 -0.6354525954175045 2116 54-55 -0.39639015639573216 2116 56-57 -0.6546494174925002 2116 58-59 -0.3634664239122216 2116 60-61 -0.8804035687087577 2116 62-63 -0.5526103734386965 2116 64-65 -1.0928609026830785 2116 66-67 -0.5100981739886592 2116 68-69 -0.6257124572787447 2116 70-71 -0.46460608334788134 2116 72-73 -0.47706944321493694 2116 74-75 -0.6601057612601622 2116 76 -0.4933351676837745 2117 1 0.23978591205226607 2117 2 -0.3420776916011725 2117 3 -0.18876956313535587 2117 4 0.03596004319712165 2117 5 -0.2423493139701236 2117 6 0.3412295354110171 2117 7 0.24079887738005823 2117 8 -0.2539245417666933 2117 9 -0.021927531143568046 2117 10-11 0.12573297814809337 2117 12-13 0.023473927630284663 2117 14-15 0.18893255107465023 2117 16-17 -0.2095377801432079 2117 18-19 0.11217544310960648 2117 20-21 -0.7036675198524378 2117 22-23 -0.151487883418298 2117 24-25 -0.11498607775442338 2117 26-27 0.21367396374518677 2117 28-29 -0.24691770646998634 2117 30-31 0.047137217518482544 2117 32-33 -0.5690550095794791 2117 34-35 -0.10544375139645012 2117 36-37 0.3342849421233822 2117 38-39 0.033465569227544734 2117 40-41 -0.06745787602624631 2117 42-43 -0.002632013606806538 2117 44-45 -0.30009265448613576 2117 46-47 -0.042873270851380596 2117 48-49 -0.18468167810765834 2117 50-51 0.2464623636456409 2117 52-53 0.024542167438042384 2117 54-55 0.038944202545053486 2117 56-57 -0.5136043889609709 2117 58-59 0.0715809831557408 2117 60-61 -0.3572204759567441 2117 62-63 -0.3439167136566894 2117 64-65 -0.24823715877943897 2117 66-67 -0.010552059840733818 2117 68-69 -0.2202865574173103 2117 70-71 0.06173457107696123 2117 72-73 -2.0627349424348225 2117 74-75 -0.6157635138705544 2117 76 -0.13807347412219073 2118 1 -0.5534440821805156 2118 2 -0.5887827279110986 2118 3 0.20144735481716225 2118 4 -1.0060676629808647 2118 5 -0.6752177390492733 2118 6 -0.5262774970619404 2118 7 -0.208434318246276 2118 8 -0.3067239956466139 2118 9 -0.6371059029053896 2118 10-11 -1.5410631566399857 2118 12-13 -0.8379292714093012 2118 14-15 -0.8672165962012244 2118 16-17 -0.47727667825179765 2118 18-19 -0.6205929384211757 2118 20-21 -0.5972447240472079 2118 22-23 -0.4248103885087531 2118 24-25 -0.2652867968909902 2118 26-27 -0.6695057733168284 2118 28-29 -0.761678249294512 2118 30-31 -0.6960039145036845 2118 32-33 -0.5346294360945478 2118 34-35 -0.79933521003138 2118 36-37 -0.6868548481382604 2118 38-39 -0.4115010633066163 2118 40-41 -0.24205269965535336 2118 42-43 -0.2538884428760255 2118 44-45 -0.8030974918859499 2118 46-47 -0.9662646294427617 2118 48-49 -0.7359086828038173 2118 50-51 -0.2206680342922951 2118 52-53 -0.3858635385751654 2118 54-55 -0.761652527796393 2118 56-57 -0.8052442792661623 2118 58-59 -0.7199294390280286 2118 60-61 -0.5568332485754155 2118 62-63 -0.48619276323120886 2118 64-65 -0.737972690716866 2118 66-67 -0.7714350324288048 2118 68-69 -0.8142462810617204 2118 70-71 -0.7005219405810621 2118 72-73 -0.766292961807963 2118 74-75 -0.77445141330913 2118 76 -0.34810843051497287 2119 1 0.4067144011937742 2119 2 0.17220829470788956 2119 3 -0.27722509615831115 2119 4 -0.1212238894716613 2119 5 0.4424235669339751 2119 6 -0.33840448437324255 2119 7 0.30829671139412795 2119 8 -0.10839408943266449 2119 9 0.19139352033549528 2119 10-11 0.05918008524777463 2119 12-13 -0.07237809030693398 2119 14-15 -0.0288382923745516 2119 16-17 0.03146623764168055 2119 18-19 -0.2041239580309906 2119 20-21 -0.2129538869941925 2119 22-23 -0.2635360016287649 2119 24-25 0.07637401859624049 2119 26-27 0.022563788847776323 2119 28-29 -0.061164181387390215 2119 30-31 -0.34843131808461436 2119 32-33 -0.008415734304087152 2119 34-35 -0.09925998058156438 2119 36-37 0.07248864952962464 2119 38-39 -0.47024397920009164 2119 40-41 -0.3907098539760341 2119 42-43 -0.1814424826974843 2119 44-45 -0.2752111886611104 2119 46-47 -0.435154744293051 2119 48-49 -0.2650814096206062 2119 50-51 -0.2835460622776438 2119 52-53 -0.12220813960831833 2119 54-55 -0.4393733262316104 2119 56-57 -0.5783685583172726 2119 58-59 -0.516863838947792 2119 60-61 -0.39433262166333094 2119 62-63 -0.24955351351958655 2119 64-65 -0.4686036150360451 2119 66-67 -0.6909608361300954 2119 68-69 -1.1291354430056444 2119 70-71 -0.5663727856585439 2119 72-73 -0.23415368701985528 2119 74-75 -0.328457381512159 2119 76 -0.22351090807152119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 209.0 14 841.0 15 1544.0 16 2028.0 17 2557.0 18 2703.0 19 2532.0 20 2212.0 21 1817.0 22 1766.0 23 1737.0 24 2000.0 25 2370.0 26 2920.0 27 3623.0 28 4352.0 29 5424.0 30 6546.0 31 8053.0 32 10054.0 33 12848.0 34 17209.0 35 24586.0 36 42911.0 37 122077.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.3466090154844 15.283295663526737 9.01759016186318 30.352505159125688 2 24.88348097089832 16.560442491541842 27.93052377409347 30.625552763466366 3 23.697233024019766 16.388120674406526 24.02046804149762 35.89417826007609 4 20.024426880799627 14.728777392500666 23.60422837729704 41.64256734940267 5 23.227647298302752 26.27574298429099 25.837743742366598 24.65886597503966 6 39.241994581163226 24.227184029873793 13.223856920247638 23.306964468715343 7 35.74712562997487 22.137913607457218 14.983224067496806 27.13173669507111 8 30.06226046916457 26.64214619628543 15.932924346861707 27.362668987688288 9 26.402439880392514 13.834177979307343 15.797453427484454 43.96592871281569 10-11 33.85386688753808 18.11783162298373 18.59145339940758 29.43684809007061 12-13 31.082503895666335 15.84711439922508 19.390064997964426 33.68031670714416 14-15 29.428600508191426 16.85507419314082 17.42029677256328 36.29602852610447 16-17 31.39433268288574 18.335953841618352 17.886899330366543 32.382814145129366 18-19 31.06776353656311 17.576825347802284 18.325074052756445 33.03033706287816 20-21 30.54834135863996 18.463879100978478 18.636551879044823 32.35122766133674 22-23 31.81899540943102 18.037461569778053 17.87444021731501 32.26910280347592 24-25 31.463471986298487 18.019913523226595 17.582967164095294 32.93364732637963 26-27 30.199135232265945 18.664453273061643 18.748508416043126 32.387903078629286 28-29 30.95317479258209 18.35350188816981 19.034717055297403 31.658606263950695 30-31 30.80910533039462 17.67983238105934 19.131231311330424 32.37983097721562 32-33 29.784299411789476 18.673051815871858 19.591867533306193 31.950781239032473 34-35 30.024532169078938 18.2197857734477 20.043905212471746 31.711776845001616 36-37 29.801847458340937 17.433282327011355 20.32923644939845 32.43563376524925 38-39 28.37308550812124 17.862332065194504 22.411136692263415 31.35344573442084 40-41 28.0284418738506 17.4759240801314 22.991801552651157 31.50383249336684 42-43 26.78481181474878 17.2207754832732 23.73268007805371 32.2617326239243 44-45 25.111956536998303 17.772135105920007 25.65945558940379 31.456452767677902 46-47 24.919980907725353 18.664628753527158 25.980935802226497 30.434454536520995 48-49 24.1959485070122 19.491317226566338 25.49081886204428 30.821915404377187 50-51 23.127096991562897 20.911129673044794 24.92752656774248 31.034246767649826 52-53 22.629434391363553 20.997817023009 25.30498504906434 31.06776353656311 54-55 22.61785268063959 20.431366080327937 25.26830963177179 31.68247160726068 56-57 22.680674687293813 21.068535650611373 25.00035096093103 31.250438701163784 58-59 23.432082040627236 20.748459281512783 24.27193154858001 31.547527129279967 60-61 23.757598304156783 19.976871674645178 24.316503586820716 31.949026434377327 62-63 23.6831945867786 20.026532646385807 23.980458495360296 32.309814271475304 64-65 24.384414526974858 20.115501242401695 22.944772787893253 32.55531144273019 66-67 24.434250979180998 20.937802703803012 21.953834599132424 32.67411171788356 68-69 24.42337119031909 21.497409908329004 22.187399098732328 31.89181980261957 70-71 25.022286019120347 21.689385537601954 22.326730588350905 30.961597854926794 72-73 24.955778922690325 21.80502716437606 21.782565664790194 31.456628248143414 74-75 24.590296264189814 22.760909200983395 21.748209657408886 30.90058487741791 76 24.793985933485885 23.154296463717657 20.919026293992953 31.132691308803505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 2.0 15 5.5 16 6.5 17 5.0 18 10.0 19 15.0 20 21.0 21 27.0 22 39.0 23 71.0 24 98.5 25 106.0 26 209.0 27 347.0 28 429.0 29 476.0 30 575.0 31 828.0 32 1190.0 33 1398.0 34 1675.5 35 2136.5 36 2544.5 37 2769.0 38 3091.0 39 3851.0 40 4678.0 41 5223.5 42 5380.0 43 5657.5 44 6190.5 45 6532.0 46 6618.0 47 6639.0 48 6368.0 49 5865.0 50 5654.0 51 5398.5 52 4776.5 53 4561.5 54 4713.0 55 5108.5 56 6040.0 57 7715.5 58 8855.0 59 10036.5 60 12632.0 61 15065.0 62 16084.0 63 16776.5 64 18100.0 65 18648.0 66 18196.0 67 17827.0 68 17209.0 69 15505.5 70 13547.0 71 12674.0 72 11249.0 73 8442.0 74 5768.0 75 4476.0 76 3339.5 77 1538.0 78 579.0 79 285.0 80 186.0 81 59.5 82 24.0 83 16.0 84 15.5 85 8.5 86 3.0 87 4.0 88 6.0 89 7.5 90 5.5 91 4.0 92 4.0 93 3.0 94 4.5 95 7.5 96 8.0 97 6.0 98 3.5 99 29.0 100 55.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.7548046551457892E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 284932.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.64194515206816 #Duplication Level Percentage of deduplicated Percentage of total 1 77.53126094864305 55.5449034445337 2 15.141116502245522 21.694780759898958 3 4.218080337947282 9.06574440654709 4 1.3560659816784884 3.8860481872798296 5 0.5731000614935939 2.0529001586085474 6 0.33112004917588866 1.4233250641085478 7 0.2137885851029775 1.072136106166004 8 0.15536228630830903 0.8904365115519829 9 0.11022671356634946 0.7107170550851822 >10 0.36939014126240266 3.639414245495821 >50 4.883925761138689E-4 0.019594060724351155 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002105765586174947 0.0 2 0.0 0.0 0.0 0.00491345303440821 0.0 3 0.0 0.0 0.0 0.005966335827495683 0.0 4 0.0 0.0 0.0 0.023163421447924416 0.0 5 0.0 0.0 0.0 0.024216304241011892 0.0 6 0.0 0.0 0.0 0.02562014796512852 0.0 7 0.0 0.0 0.0 0.026673030758215994 0.0 8 0.0 0.0 0.0 0.028427835413361784 0.0 9 0.0 0.0 0.0 0.030182640068507575 0.0 10 0.0 0.0 0.0 0.031235522861595047 0.0 11 0.0 0.0 0.0 0.03228840565468252 0.0 12 0.0 0.0 0.0 0.03228840565468252 0.0 13 0.0 0.0 0.0 0.03263936658571168 0.0 14 0.0 0.0 0.0 0.03544705403394494 0.0 15 0.0 0.0 0.0 0.03755281962011989 0.0 16 0.0 0.0 0.0 0.03790378055114905 0.0 17 0.0 0.0 0.0 0.04071146799938231 0.0 18 0.0 0.0 0.0 0.04316819451658641 0.0 19 0.0 0.0 0.0 0.048432608482023784 0.0 20 0.0 0.0 0.0 0.051591256861286204 0.0 21 0.0 0.0 0.0 0.05510086617157778 0.0 22 0.0 0.0 0.0 0.06001431920598599 0.0 23 0.0 0.0 0.0 0.06562969410245252 0.0 24 0.0 0.0 0.0 0.07510563924023977 0.0 25 0.0 0.0 0.0 0.0873892718262603 0.0 26 0.0 0.0 0.0 0.11792287282579703 0.0 27 0.0 0.0 0.0 0.3530666966153328 0.0 28 0.0 0.0 0.0 0.589263403197956 0.0 29 0.0 0.0 0.0 0.8426571954010079 0.0 30 0.0 0.0 0.0 1.1100894248452262 0.0 31 0.0 0.0 0.0 1.3810312635997362 0.0 32 0.0 0.0 0.0 1.694439375008774 0.0 33 0.0 0.0 0.0 2.1001502112784807 0.0 34 0.0 0.0 0.0 2.6560723260286663 0.0 35 0.0 0.0 0.0 3.393090281189898 0.0 36 0.0 0.0 0.0 4.514761416759087 0.0 37 0.0 0.0 0.0 5.989148288012578 0.0 38 0.0 0.0 0.0 7.6197127735740455 0.0 39 0.0 0.0 0.0 9.254137829376834 0.0 40 0.0 0.0 0.0 10.792750551008663 0.0 41 0.0 0.0 0.0 12.38541125601898 0.0 42 0.0 0.0 0.0 13.937360493029916 0.0 43 0.0 0.0 0.0 15.426487723386632 0.0 44 0.0 0.0 0.0 16.82050454143445 0.0 45 0.0 0.0 0.0 18.291732764308676 0.0 46 0.0 0.0 0.0 19.664340965563714 0.0 47 0.0 0.0 0.0 21.02045400306038 3.5096093102915784E-4 48 0.0 0.0 0.0 22.284264315696376 3.5096093102915784E-4 49 0.0 0.0 0.0 23.542459253435908 3.5096093102915784E-4 50 0.0 0.0 0.0 24.781000379037806 3.5096093102915784E-4 51 0.0 0.0 0.0 25.944084904468436 3.5096093102915784E-4 52 0.0 0.0 0.0 27.10365982058877 3.5096093102915784E-4 53 0.0 0.0 0.0 28.26393665857117 3.5096093102915784E-4 54 0.0 0.0 0.0 29.318223295382758 3.5096093102915784E-4 55 0.0 0.0 0.0 30.37637050243567 3.5096093102915784E-4 56 0.0 0.0 0.0 31.381873569834205 3.5096093102915784E-4 57 0.0 0.0 0.0 32.37614588743981 3.5096093102915784E-4 58 0.0 0.0 0.0 33.34234133056308 3.5096093102915784E-4 59 0.0 0.0 0.0 34.292392570859015 3.5096093102915784E-4 60 0.0 0.0 0.0 35.22980921763789 3.5096093102915784E-4 61 0.0 0.0 0.0 36.187581598416465 3.5096093102915784E-4 62 0.0 0.0 0.0 37.09376272233375 3.5096093102915784E-4 63 0.0 0.0 0.0 38.01573708814735 3.5096093102915784E-4 64 0.0 0.0 0.0 38.88962980640995 3.5096093102915784E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAGAGT 20 7.9184014E-5 70.0 23 CAGAGTA 20 7.9184014E-5 70.0 24 ATTCGCG 20 0.0069466983 52.5 62 TCTAACG 20 0.0069466983 52.5 11 GTTATTG 20 0.0069466983 52.5 34 TAGTAAA 20 0.0069466983 52.5 33 AATTATC 35 0.0012541797 40.0 30 CATTTCC 45 9.184484E-5 38.888885 49 TTCCGTT 50 1.707262E-4 35.000004 21 AATTTCA 40 0.0024163907 35.0 16 AGAGTAC 40 0.0024163907 35.0 25 GAAAGCA 40 0.0024163907 35.0 33 AGATTGC 40 0.0024163907 35.0 27 CTGCACT 55 2.9878467E-4 31.81818 4 AGTGCGA 45 0.0043030772 31.111109 10 CAATATA 45 0.0043030772 31.111109 36 TAACTCT 45 0.0043030772 31.111109 13 ATTTCCG 45 0.0043030772 31.111109 50 TCTTAGG 60 4.975E-4 29.166666 2 GAGTCTC 50 0.0072014113 28.000002 35 >>END_MODULE