FastQCFastQC Report
Wed 25 May 2016
SRR1780174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284932
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT69082.4244381115494225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC20900.7335083458509399No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG18850.6615613549899625RNA PCR Primer, Index 47 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG17240.6050566450942682No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4040.14178821613577977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGAT150.002223182570.069
CTATTTG150.002223182570.015
CTTTATA401.0633812E-861.252
CTTAATA308.363992E-658.3333321
TTAATAC252.387975E-456.0000042
CATCGAT252.387975E-456.00000429
CTTTTCG200.006946698352.548
AATATGT200.006946698352.541
CATTAAG200.006946698352.548
ACTTTTC200.006946698352.547
TAGTAAC200.006946698352.531
TCTTTAT506.179471E-849.0000041
TTTATAC654.852973E-737.692313
TATGCCG16400.033.29268343
CTTGAAA16000.033.25000457
GTATGCC16500.033.09090842
TTGAAAA16200.033.05555358
CGTATGC16550.032.99093641
TCGTATG16650.032.79279340
CTCGTAT16650.032.5825839