##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780174_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 284932 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.70210085213314 34.0 34.0 34.0 34.0 34.0 2 33.66970013898053 34.0 34.0 34.0 34.0 34.0 3 33.67562084988699 34.0 34.0 34.0 34.0 34.0 4 33.71186809484368 34.0 34.0 34.0 34.0 34.0 5 33.687922030519566 34.0 34.0 34.0 34.0 34.0 6 37.368691477264754 38.0 38.0 38.0 36.0 38.0 7 37.3636937936069 38.0 38.0 38.0 36.0 38.0 8 37.24723442786349 38.0 38.0 38.0 36.0 38.0 9 37.26462103238668 38.0 38.0 38.0 36.0 38.0 10-11 37.225481869358305 38.0 38.0 38.0 36.0 38.0 12-13 37.20450317970604 38.0 38.0 38.0 36.0 38.0 14-15 37.107669549225776 38.0 38.0 38.0 36.0 38.0 16-17 37.16282130473236 38.0 38.0 38.0 36.0 38.0 18-19 37.115394199317734 38.0 38.0 38.0 35.5 38.0 20-21 37.152634663709236 38.0 38.0 38.0 36.0 38.0 22-23 37.09672132298232 38.0 38.0 38.0 36.0 38.0 24-25 37.119781210955594 38.0 38.0 38.0 36.0 38.0 26-27 37.014787738828915 38.0 38.0 38.0 35.5 38.0 28-29 36.95911656114441 38.0 38.0 38.0 35.0 38.0 30-31 36.93126605646259 38.0 38.0 38.0 35.0 38.0 32-33 36.92300970056013 38.0 38.0 38.0 35.0 38.0 34-35 36.90670054609521 38.0 38.0 38.0 35.0 38.0 36-37 36.92724579899766 38.0 38.0 38.0 35.0 38.0 38-39 36.62609675290946 38.0 37.0 38.0 34.0 38.0 40-41 36.75433787710752 38.0 37.0 38.0 34.0 38.0 42-43 36.808201605997226 38.0 37.0 38.0 34.5 38.0 44-45 36.87245904285935 38.0 37.0 38.0 35.0 38.0 46-47 36.8305648365224 38.0 37.5 38.0 34.5 38.0 48-49 36.87147284264316 38.0 37.0 38.0 35.0 38.0 50-51 36.74414948127975 38.0 37.0 38.0 34.0 38.0 52-53 36.765286103350974 38.0 37.0 38.0 34.0 38.0 54-55 36.73572290932573 38.0 37.0 38.0 34.5 38.0 56-57 36.772017534008114 38.0 37.0 38.0 34.5 38.0 58-59 36.67415032358598 38.0 37.0 38.0 34.0 38.0 60-61 36.69310923308018 38.0 37.0 38.0 34.0 38.0 62-63 36.61801061305856 38.0 37.0 38.0 34.0 38.0 64-65 36.607085199275616 38.0 37.0 38.0 34.0 38.0 66-67 36.39077920345907 38.0 37.0 38.0 34.0 38.0 68-69 36.36927933682422 38.0 37.0 38.0 34.0 38.0 70-71 36.037305392163745 38.0 37.0 38.0 32.5 38.0 72-73 35.644885446352106 38.0 37.0 38.0 30.5 38.0 74-75 35.512090604073954 38.0 37.0 38.0 29.5 38.0 76 34.49849086799657 38.0 35.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04098078980783981 1101 2 -0.06521119594776081 1101 3 0.026372777505017098 1101 4 0.11535821834743132 1101 5 0.0035998408860535847 1101 6 -0.06298136337710503 1101 7 -0.08700228922992892 1101 8 -0.06874990339866116 1101 9 0.12468377042132062 1101 10-11 0.1598542140664705 1101 12-13 0.08712987264193117 1101 14-15 0.2043889526673155 1101 16-17 0.16041444221188073 1101 18-19 0.10629733647567718 1101 20-21 0.20775969989727372 1101 22-23 0.1663838931981445 1101 24-25 0.03762278508675365 1101 26-27 0.13351761020901876 1101 28-29 -0.08353078045901441 1101 30-31 -0.03878015292774961 1101 32-33 -0.04002088474756249 1101 34-35 0.14534681020395368 1101 36-37 0.17439859871145558 1101 38-39 0.11757406671767967 1101 40-41 0.28567377152334217 1101 42-43 0.14128461242970047 1101 44-45 0.0796448331590085 1101 46-47 0.0685781465542803 1101 48-49 0.1325896734050218 1101 50-51 0.2306307783516388 1101 52-53 0.22081607808099335 1101 54-55 -0.042134752493602434 1101 56-57 -0.012865901071997143 1101 58-59 0.018213103374272066 1101 60-61 -0.002895637379694449 1101 62-63 0.046985106983242986 1101 64-65 -0.07943975659888025 1101 66-67 0.10817555753214947 1101 68-69 -0.37349598849095145 1101 70-71 0.13471034477439048 1101 72-73 0.6208275079144627 1101 74-75 0.5878767041163968 1101 76 -0.5935117863668111 1102 1 0.038776554343662895 1102 2 0.11471933934463863 1102 3 -0.02733530129695083 1102 4 0.09178288804493917 1102 5 -0.08467273435953615 1102 6 0.09725652343997382 1102 7 0.10850603618116139 1102 8 -0.018557416505657898 1102 9 -0.0509201238693322 1102 10-11 0.06331113433893165 1102 12-13 0.06814708821784166 1102 14-15 0.20492782184211933 1102 16-17 0.17992825200910545 1102 18-19 0.13180694827023842 1102 20-21 0.20684746576293378 1102 22-23 0.18992392639311362 1102 24-25 0.024293812907153267 1102 26-27 0.08534960852730222 1102 28-29 0.2698897445736961 1102 30-31 0.18202913915346386 1102 32-33 0.11360703040132591 1102 34-35 0.15668580989961356 1102 36-37 0.20648131630635902 1102 38-39 0.08319154647283256 1102 40-41 0.13243177141666962 1102 42-43 0.04993491378856163 1102 44-45 0.19014203605932778 1102 46-47 0.08451439840933972 1102 48-49 0.1747571668083765 1102 50-51 0.09407646630010191 1102 52-53 0.20242942258389718 1102 54-55 0.15888776549223138 1102 56-57 0.06379314141944548 1102 58-59 0.24191205199110044 1102 60-61 0.2940126497536468 1102 62-63 0.28509939431111064 1102 64-65 0.011411076255988917 1102 66-67 0.21040931537203278 1102 68-69 0.06103493648151925 1102 70-71 0.01795143500007157 1102 72-73 0.08539470262585525 1102 74-75 -0.10088202995675744 1102 76 0.21863857617370286 1103 1 -0.05184325457948802 1103 2 -0.047237736196741764 1103 3 0.005011796523660905 1103 4 0.02607572582859774 1103 5 0.07998860016363096 1103 6 0.1065782277562306 1103 7 -0.010629083309702025 1103 8 -0.04979658213473215 1103 9 0.023154564326894445 1103 10-11 0.008828003548671859 1103 12-13 -0.0497474486966496 1103 14-15 0.1318698686978479 1103 16-17 0.03759891125136505 1103 18-19 0.22451373966846688 1103 20-21 0.21713992971368867 1103 22-23 0.22848222774463522 1103 24-25 -0.024744622541852834 1103 26-27 0.12820924934624145 1103 28-29 0.1375679709174591 1103 30-31 0.18713391788357825 1103 32-33 0.19264178781693886 1103 34-35 -0.037622885634590375 1103 36-37 0.19252137069301511 1103 38-39 0.11264520777419307 1103 40-41 0.2025472978427345 1103 42-43 0.12760698997811915 1103 44-45 -0.04758629595252728 1103 46-47 0.08381421541210443 1103 48-49 0.08443958972794974 1103 50-51 -0.11065809726505904 1103 52-53 0.0897074969826761 1103 54-55 0.1852459999156082 1103 56-57 -0.1627458291532733 1103 58-59 0.27204856129621646 1103 60-61 0.1541637204532904 1103 62-63 0.19109473948851274 1103 64-65 0.29685661573605415 1103 66-67 -0.04861317188297676 1103 68-69 -0.03929322248188072 1103 70-71 -0.03461127999153035 1103 72-73 0.2097491625428276 1103 74-75 0.2881619526901602 1103 76 0.15235257591442775 1104 1 -0.002943499078263301 1104 2 0.06523170145607082 1104 3 0.013569253736378073 1104 4 0.13976765736893526 1104 5 0.06776366128832478 1104 6 0.1383630688320281 1104 7 0.09572788490545747 1104 8 -0.16226601978754474 1104 9 0.0048171560139351755 1104 10-11 -0.03762676417748878 1104 12-13 0.026658419274006917 1104 14-15 -0.022164361131004284 1104 16-17 -0.04247443838188758 1104 18-19 0.05336459541419458 1104 20-21 0.014817191327374246 1104 22-23 0.005377093270311661 1104 24-25 0.001538996040046925 1104 26-27 0.17283027624110758 1104 28-29 0.16568533033066757 1104 30-31 -0.032914981871925875 1104 32-33 -0.007847209738073957 1104 34-35 0.021668067910646016 1104 36-37 -0.10393339703314552 1104 38-39 1.7577012137337533E-4 1104 40-41 -0.25894414470004534 1104 42-43 -0.012979807087894812 1104 44-45 0.024540843411784863 1104 46-47 -0.1282884740744521 1104 48-49 0.13150560439787995 1104 50-51 -0.25246738821860504 1104 52-53 -0.14990130497331933 1104 54-55 -0.26646549152693666 1104 56-57 -0.45064313966672387 1104 58-59 0.09478694760428397 1104 60-61 -0.10255999592812515 1104 62-63 -0.04729156857995065 1104 64-65 -0.22453702729573166 1104 66-67 -0.6036253968218546 1104 68-69 -0.4060413887410448 1104 70-71 -0.4196968545636608 1104 72-73 -0.1857276100733074 1104 74-75 -0.14949085904578396 1104 76 -0.2266205292200425 1105 1 -0.038179908717111743 1105 2 0.11656123958449882 1105 3 0.0977145419651606 1105 4 0.025549627712813106 1105 5 0.0997887437002305 1105 6 0.07533468994407144 1105 7 0.12772559805122086 1105 8 0.10153417240552187 1105 9 0.09262080281659735 1105 10-11 0.06650417039171685 1105 12-13 0.14767162950646906 1105 14-15 0.27055146073765 1105 16-17 0.2550842230785406 1105 18-19 0.175545561001897 1105 20-21 0.2305797599025894 1105 22-23 0.12768426158521606 1105 24-25 -0.03277902266836463 1105 26-27 0.1810471381418708 1105 28-29 0.17628179787071474 1105 30-31 0.1245966652713335 1105 32-33 0.24774828467251098 1105 34-35 0.12365659714710375 1105 36-37 0.16347041520152317 1105 38-39 0.032080731865221424 1105 40-41 0.12390627313741476 1105 42-43 -0.02598020850379612 1105 44-45 0.06758205544453233 1105 46-47 0.08211732091150736 1105 48-49 0.006075298713589916 1105 50-51 -0.02784782101792871 1105 52-53 0.17843257574534022 1105 54-55 -0.011586159580311062 1105 56-57 0.2065902532707895 1105 58-59 0.22866371292194998 1105 60-61 0.3678887992159545 1105 62-63 0.18546731426742724 1105 64-65 -0.0762685895334414 1105 66-67 -0.10255039286851542 1105 68-69 0.13885637184477417 1105 70-71 -0.018744708204984306 1105 72-73 0.31397436902874176 1105 74-75 0.5698418782457537 1105 76 0.34702408390017325 1106 1 -0.0012505847487176425 1106 2 -0.011401885964225755 1106 3 0.04099446587502342 1106 4 -0.08236667313587986 1106 5 -0.05167818635537458 1106 6 0.05998862614541878 1106 7 0.041758375341245824 1106 8 0.0695433910183425 1106 9 0.20765226316193264 1106 10-11 0.15280062927377713 1106 12-13 0.1810342282773192 1106 14-15 0.0424090843841185 1106 16-17 0.0852806673278792 1106 18-19 0.10400071171889635 1106 20-21 0.12248055006940461 1106 22-23 0.19372355043508094 1106 24-25 0.0594711236588239 1106 26-27 -0.01228765846334312 1106 28-29 0.1151810516020575 1106 30-31 0.09491053708871533 1106 32-33 0.17763927091775145 1106 34-35 0.0735761171939302 1106 36-37 0.04146097709792684 1106 38-39 0.0856364825236966 1106 40-41 0.16871897433729544 1106 42-43 0.20709156498171666 1106 44-45 -0.0706788459309422 1106 46-47 -0.03888611777740891 1106 48-49 0.19709629666500916 1106 50-51 0.11825987579213404 1106 52-53 0.26089709140234874 1106 54-55 0.1624425937120506 1106 56-57 0.20025245695823912 1106 58-59 0.24738444748443555 1106 60-61 0.30880919722590505 1106 62-63 0.2329321957424071 1106 64-65 0.26788381023243346 1106 66-67 0.2598837598970647 1106 68-69 0.27280976663553247 1106 70-71 0.24545817444060702 1106 72-73 0.30749024552859083 1106 74-75 0.36238650803797157 1106 76 0.8844106803297933 1107 1 0.08579607602084849 1107 2 -0.007863628555995206 1107 3 0.020824555805731393 1107 4 0.09764217250764062 1107 5 0.0017918858840459961 1107 6 0.004859995745889023 1107 7 0.0426060828452961 1107 8 0.05523371978142677 1107 9 0.07892050730169586 1107 10-11 -0.0540584301394631 1107 12-13 0.1284210777539485 1107 14-15 0.19014054538958192 1107 16-17 0.1742945623595773 1107 18-19 0.021022125547588644 1107 20-21 0.13954527069449796 1107 22-23 0.002754399616861747 1107 24-25 0.17976726843252067 1107 26-27 0.041173199564077834 1107 28-29 0.14298493631383025 1107 30-31 0.04484972328950931 1107 32-33 0.16203961000076106 1107 34-35 0.04619147912817567 1107 36-37 0.13155293493369413 1107 38-39 0.20300711498720858 1107 40-41 0.1924732128748161 1107 42-43 0.10015602760887532 1107 44-45 0.15738211529564694 1107 46-47 -0.033647838255070894 1107 48-49 -0.011702354374349966 1107 50-51 0.07790162722955074 1107 52-53 0.21553091861744633 1107 54-55 0.2490607936235989 1107 56-57 0.045356946122325326 1107 58-59 0.20656088121582172 1107 60-61 -0.19119080277409495 1107 62-63 0.23670080845064945 1107 64-65 0.33768607324289945 1107 66-67 0.3860171079731387 1107 68-69 0.3225619043327157 1107 70-71 0.3430412866328538 1107 72-73 0.4711116151290611 1107 74-75 0.4995308394547493 1107 76 0.215753743870998 1108 1 -0.09932455539740204 1108 2 0.02717310013378693 1108 3 0.008423021761004179 1108 4 0.021499287551719704 1108 5 0.025068326059702883 1108 6 -0.25539452464880696 1108 7 -0.069052533485916 1108 8 0.07638709644132291 1108 9 0.02922784160539038 1108 10-11 -0.04642992608030028 1108 12-13 -0.12359150611015224 1108 14-15 -0.06079350984715859 1108 16-17 0.050458461626860185 1108 18-19 -0.0704125338505932 1108 20-21 -0.017566837566285187 1108 22-23 -0.1873880341494285 1108 24-25 -0.041057605095318195 1108 26-27 -0.01021142723102031 1108 28-29 -0.053784731381917084 1108 30-31 0.03406366443089581 1108 32-33 -0.17060998562826057 1108 34-35 0.009953380799736067 1108 36-37 0.06899672937663581 1108 38-39 -0.1529366902719289 1108 40-41 -0.12095910404571697 1108 42-43 -0.032679142931534955 1108 44-45 0.10937754777326347 1108 46-47 0.0771161246732106 1108 48-49 0.0563847870303249 1108 50-51 0.005297181466559664 1108 52-53 -0.15515380799563872 1108 54-55 -0.019287326986187736 1108 56-57 0.09893494166605876 1108 58-59 -0.04908671496328765 1108 60-61 0.1275514906907631 1108 62-63 -0.08974647498605748 1108 64-65 0.01757630436942037 1108 66-67 0.06059427221543956 1108 68-69 0.2046847515246739 1108 70-71 0.29230408604815494 1108 72-73 -0.058255112417299415 1108 74-75 0.07667653971031996 1108 76 0.25686299047703187 1109 1 0.061449495283291355 1109 2 -0.004999324939817029 1109 3 0.046021476679342754 1109 4 0.040982666600015705 1109 5 0.11381301012321643 1109 6 -0.12466023339840149 1109 7 0.06681839935084355 1109 8 0.01969845304314788 1109 9 0.05549139881619425 1109 10-11 -0.05980941474382462 1109 12-13 -0.03584002142256537 1109 14-15 -0.059456661914396136 1109 16-17 0.029871003462332624 1109 18-19 0.17571689819349245 1109 20-21 0.12388111029350313 1109 22-23 -0.09635498097748041 1109 24-25 0.044440111253862824 1109 26-27 -0.043950323529365676 1109 28-29 -0.012369968806105192 1109 30-31 0.2115947107581846 1109 32-33 0.013286029621241369 1109 34-35 0.07724008278015759 1109 36-37 0.0566670595308949 1109 38-39 -0.06167244104572944 1109 40-41 -0.034399773161702285 1109 42-43 0.03909936810296699 1109 44-45 -0.02569835373407159 1109 46-47 0.10409607509974705 1109 48-49 0.06884499614480433 1109 50-51 0.06861501784895552 1109 52-53 -0.020953148693685364 1109 54-55 -0.23098977923712738 1109 56-57 0.159336090411621 1109 58-59 0.18067026181016388 1109 60-61 0.19210001355244088 1109 62-63 -0.09733741210072822 1109 64-65 -0.06527445307288815 1109 66-67 -0.046101134060521076 1109 68-69 -0.03994033340448766 1109 70-71 0.32460233210367306 1109 72-73 -0.18965861401522943 1109 74-75 0.06704337078306821 1109 76 0.5019827091413163 1110 1 -0.021896820342192314 1110 2 0.03656997453570199 1110 3 0.042912217708583 1110 4 0.05683404531944092 1110 5 0.13209856862142288 1110 6 -0.16349026922952703 1110 7 0.014778881253533882 1110 8 0.17298037649955234 1110 9 0.10178300706254362 1110 10-11 -0.02844095132793001 1110 12-13 -0.008926107001833827 1110 14-15 -0.07105633995355021 1110 16-17 0.06753912414572483 1110 18-19 0.17309122960544698 1110 20-21 0.21030422915936953 1110 22-23 0.04742715019560251 1110 24-25 0.11141413368726205 1110 26-27 0.21303414964921785 1110 28-29 0.2771562424417553 1110 30-31 -0.008245801782713613 1110 32-33 0.08338878446363651 1110 34-35 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54-55 0.07842526585067588 2115 56-57 0.1285755303736522 2115 58-59 -0.11702788721688506 2115 60-61 0.019565011179395242 2115 62-63 -0.13585485396119168 2115 64-65 -0.05520385506624592 2115 66-67 0.11370842929971303 2115 68-69 0.032570938546150785 2115 70-71 -0.4061463627230921 2115 72-73 0.024701257840490598 2115 74-75 -0.13426476875181237 2115 76 0.056948300028835774 2116 1 -0.2571561951680721 2116 2 -0.012790178507522398 2116 3 -0.19562532802794408 2116 4 -0.2593525039645641 2116 5 -0.04594790154072115 2116 6 0.0136758309543481 2116 7 0.006528663018357861 2116 8 0.08212210863677427 2116 9 -0.28256349467405073 2116 10-11 -0.09039745287878986 2116 12-13 -0.013051216119414732 2116 14-15 0.0986581898657306 2116 16-17 -0.5424699767253571 2116 18-19 -0.254346039390299 2116 20-21 -0.3180375549828298 2116 22-23 -0.09435699200086844 2116 24-25 -0.30192235709114357 2116 26-27 -0.17970929865226282 2116 28-29 -0.09097770752202905 2116 30-31 -0.16200409007601735 2116 32-33 -0.2750174487044035 2116 34-35 -0.14550217105567498 2116 36-37 -0.3481957067434962 2116 38-39 -0.251371532361091 2116 40-41 -0.417029558652537 2116 42-43 -0.14151566988303443 2116 44-45 -0.3444390434406088 2116 46-47 -0.12890239801213 2116 48-49 -0.131321658108142 2116 50-51 -0.3613808856219194 2116 52-53 -0.19944264668266243 2116 54-55 -0.2157087945804932 2116 56-57 -0.2791852487809905 2116 58-59 -0.1876207407222381 2116 60-61 -0.4349864232120524 2116 62-63 -0.10376989385255087 2116 64-65 -0.13588837382366847 2116 66-67 -0.18543433084288097 2116 68-69 -0.1597945342088991 2116 70-71 -0.19455452772054826 2116 72-73 -0.6255470022172247 2116 74-75 -0.31954422045780717 2116 76 -0.3769890620489136 2117 1 -0.14208303880657525 2117 2 -0.0030927327065128907 2117 3 -0.0854838160206981 2117 4 -0.02538962222583052 2117 5 -0.009379540439610423 2117 6 0.11438830081349494 2117 7 0.0876345733540802 2117 8 -0.7442425493667812 2117 9 -0.08897681447809447 2117 10-11 0.1155303399921479 2117 12-13 -0.05172098142988091 2117 14-15 -0.019475422781972895 2117 16-17 0.062141983603268613 2117 18-19 -0.0030318434556306784 2117 20-21 -0.025809259127818507 2117 22-23 0.13423781667970047 2117 24-25 -0.17886473759150334 2117 26-27 0.10341400336731255 2117 28-29 -0.5869396509390512 2117 30-31 -0.006728212581556647 2117 32-33 -0.15979917979885272 2117 34-35 -0.034618730251779084 2117 36-37 0.002665694410858066 2117 38-39 -0.052025084345203254 2117 40-41 -0.2230751872735368 2117 42-43 -0.07340005991936494 2117 44-45 -0.1688004936931904 2117 46-47 -0.0711912483498125 2117 48-49 -0.27037875389333266 2117 50-51 0.11524835728339156 2117 52-53 0.11655028317233018 2117 54-55 0.14710747984587869 2117 56-57 -0.1404860245330184 2117 58-59 0.05847485898535609 2117 60-61 -0.18147480875317257 2117 62-63 0.18755608565712123 2117 64-65 -0.02909972533425531 2117 66-67 0.053995911484157944 2117 68-69 0.08681277815403377 2117 70-71 0.19593023546232757 2117 72-73 -0.16412839071514895 2117 74-75 0.15775712265153174 2117 76 -0.7650221869875367 2118 1 -0.18341632506059113 2118 2 -0.31865833184966874 2118 3 -0.3971771413505465 2118 4 -0.26485856753143366 2118 5 -0.07634045283676727 2118 6 -0.12187218904679753 2118 7 -0.06345237037436391 2118 8 -0.09089932937690293 2118 9 -0.1904892375328373 2118 10-11 0.08846914865003441 2118 12-13 -0.003947244626807844 2118 14-15 -0.037027769097427665 2118 16-17 -0.15317174119458343 2118 18-19 -0.2510527466723289 2118 20-21 -0.09096703368850712 2118 22-23 -0.13643412287046175 2118 24-25 -0.09933600155346767 2118 26-27 -0.23487913599922905 2118 28-29 -0.0384114680829839 2118 30-31 -0.06013616663214094 2118 32-33 -0.38829982125454876 2118 34-35 -0.3241388743144924 2118 36-37 -0.2075152078642475 2118 38-39 -0.339842134119813 2118 40-41 -0.25888253870886757 2118 42-43 -0.14146969022903022 2118 44-45 -0.2915739362505434 2118 46-47 -0.159961654291223 2118 48-49 -0.05429999019619203 2118 50-51 -0.266277718773253 2118 52-53 -0.15407222309510615 2118 54-55 0.10439006167561615 2118 56-57 -0.2605347516259826 2118 58-59 -0.41696150743296556 2118 60-61 -0.2557577319079556 2118 62-63 -0.012839380604660278 2118 64-65 -0.12121511673992558 2118 66-67 -0.17835346412640263 2118 68-69 -0.3371553020691209 2118 70-71 -0.22853923948559185 2118 72-73 -0.8190063964352845 2118 74-75 -0.7529369441775629 2118 76 -0.7289288287021662 2119 1 -0.029639806679533365 2119 2 -0.05679966290939831 2119 3 0.043827924099076654 2119 4 0.058791063844260805 2119 5 -0.15856122619551627 2119 6 -0.03454584369347202 2119 7 -0.08216444219341668 2119 8 0.15439883000922094 2119 9 0.05701063708475118 2119 10-11 -0.013054247708858213 2119 12-13 -0.11392643114568557 2119 14-15 -0.8426524242957782 2119 16-17 -0.22069769535477235 2119 18-19 -0.21206257665377137 2119 20-21 0.08498211120340926 2119 22-23 -0.08520035989337771 2119 24-25 -0.11725314885273264 2119 26-27 0.029769291705264322 2119 28-29 0.1018450643409139 2119 30-31 -0.047987864310066186 2119 32-33 -0.33442048929205725 2119 34-35 -0.005542154318497694 2119 36-37 -0.1039781698984541 2119 38-39 0.12185479150333833 2119 40-41 -0.04167505588625886 2119 42-43 0.007613764307308202 2119 44-45 0.07083014620093309 2119 46-47 -0.01129697702556598 2119 48-49 -0.07933760492110764 2119 50-51 -0.0060063912242185324 2119 52-53 -0.0446597650982099 2119 54-55 -0.013352804064886925 2119 56-57 0.14955853289179544 2119 58-59 -0.08378281241315477 2119 60-61 -0.12192328685052445 2119 62-63 -0.20820524649563765 2119 64-65 -0.3002323693604225 2119 66-67 -0.22746324897017445 2119 68-69 -0.3359115084987252 2119 70-71 -0.08718435709429428 2119 72-73 -0.2657464536096654 2119 74-75 -0.11966045087298482 2119 76 -1.0400928790557487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 3.0 19 3.0 20 3.0 21 7.0 22 17.0 23 24.0 24 64.0 25 138.0 26 284.0 27 547.0 28 1066.0 29 1685.0 30 2717.0 31 4328.0 32 6352.0 33 9770.0 34 14747.0 35 23893.0 36 41198.0 37 178084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6545140594949 14.226903261128971 9.416281779512305 31.70230089986383 2 24.632192944281442 16.262827622029118 28.731065657770976 30.373913775918464 3 24.089958305841392 15.156949728356238 23.952732581808995 36.800359383993374 4 20.12339786334985 15.344011904594781 24.163309140426488 40.36928109162888 5 23.251161680681705 25.599441270197804 28.026336108264427 23.123060940856064 6 39.87021464770542 26.412617747392357 12.65249252453217 21.064675080370055 7 35.85136102649053 21.027122260749934 15.67742478907248 27.444091923687054 8 29.972414470821107 27.706610700096867 14.792652281947975 27.528322547134053 9 26.06270969915629 12.885179621804502 14.633666980191764 46.41844369884745 10-11 34.11954431232715 19.084378027038028 18.208204062723738 28.587873597911077 12-13 31.307996293852568 15.188185251217835 19.780158072803335 33.72366038212626 14-15 28.76423146575323 15.30189659287128 16.70591579745343 39.22795614392206 16-17 32.493717799334576 19.006640180815072 16.539911277076634 31.95973074277371 18-19 33.06280094899836 18.030968792554013 16.74364409753906 32.16258616090857 20-21 30.898249406876026 17.61402720649137 17.049155587999945 34.43856779863266 22-23 31.093910125924783 18.846601996265775 17.00756671767299 33.05192116013645 24-25 32.92890935381073 18.549338087684077 16.683103336936533 31.838649221568655 26-27 31.982718683756122 19.316363202448304 17.743707270506647 30.957210843288923 28-29 30.86315331377311 18.61180913340727 18.885207698678983 31.63982985414064 30-31 31.8584785141718 16.872973200623306 20.030393216627125 31.23815506857777 32-33 30.16825067033538 17.385200679460365 20.19113332303848 32.25541532716578 34-35 29.681116898066907 20.198152541659063 19.60678337287493 30.513947187399097 36-37 31.151643199079075 19.323557901534404 19.020854098521752 30.50394480086477 38-39 28.490481939550488 18.19135793803434 22.135807841871046 31.18235228054413 40-41 27.913151208007527 16.34618084314854 23.383298471214182 32.357369477629746 42-43 28.567342383445876 16.12349613241054 23.828492412224673 31.480669071918914 44-45 25.209523675824407 18.61268653573484 26.198180618533545 29.979609169907206 46-47 25.94671711145115 18.045884632122753 25.642083023317845 30.365315233108248 48-49 25.329376833770866 18.291732764308676 23.961331124619207 32.41755927730125 50-51 23.696004660761165 19.562035854168712 25.107569525360436 31.634389959709686 52-53 21.848897280754706 19.610819423581766 27.91630985638679 30.623973439276742 54-55 21.64095292911993 19.648898684598432 26.26135358611879 32.448794800162844 56-57 22.80754706386085 20.116905086125815 24.108383754720425 32.96716409529291 58-59 22.5373071469684 20.35099602712226 25.304283127202282 31.80741369870706 60-61 23.93641289851614 21.773791641514467 22.595391181053724 31.694404278915673 62-63 21.86626984684065 24.679397189504865 21.030982830991256 32.42335013266323 64-65 21.697106678084594 25.619972484663005 20.16709249926298 32.51582833798942 66-67 22.199858211783866 25.810368789746324 19.652583774374236 32.337189224095575 68-69 22.026658992320975 26.703739839681045 20.08654696559179 31.18305420240619 70-71 21.919791388822595 25.934608959330646 20.99816798394003 31.147431667906726 72-73 21.901541420409078 24.361602066457962 22.0582454761136 31.678611037019362 74-75 21.52513582188031 24.295094970027936 22.94617663161737 31.23359257647439 76 21.44862633891595 25.097216177895078 22.918801678997095 30.535355804191877 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 2.5 17 4.0 18 7.0 19 12.0 20 12.5 21 11.0 22 23.5 23 46.0 24 68.5 25 81.0 26 141.5 27 258.0 28 357.0 29 400.0 30 474.5 31 678.0 32 964.5 33 1122.0 34 1246.5 35 1620.5 36 2022.0 37 2174.0 38 2556.5 39 4846.5 40 7885.0 41 8844.5 42 8673.0 43 7861.0 44 6793.5 45 6308.0 46 6078.0 47 5995.5 48 5555.5 49 5008.5 50 4819.0 51 4509.5 52 4005.0 53 3979.5 54 4149.0 55 4730.0 56 5894.5 57 7501.5 58 8525.0 59 9712.5 60 12286.0 61 14604.5 62 15537.0 63 16163.5 64 17295.5 65 17942.5 66 18171.0 67 18258.0 68 17631.0 69 16022.5 70 13864.5 71 12688.0 72 11291.0 73 8494.0 74 5769.0 75 4444.0 76 3315.0 77 1481.0 78 498.0 79 220.0 80 131.0 81 28.0 82 10.5 83 7.0 84 4.5 85 3.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 284932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.452531025092775 #Duplication Level Percentage of deduplicated Percentage of total 1 69.70591661711444 42.836050035457546 2 20.165721327115417 24.784692309958707 3 5.7982775836373275 10.689564993017186 4 1.9082110086132915 4.690575848369275 5 0.764100496494543 2.347795473355985 6 0.4494447251777645 1.6571709550830525 7 0.30279388136255647 1.3025215272048534 8 0.18841000525014368 0.9262617354457878 9 0.1587733040171248 0.8781319251961964 >10 0.5475317884633853 4.825816616920829 >50 0.005124913936111322 0.22220691359886688 >100 0.0034166092907408816 0.42761708412254384 >500 0.0 0.0 >1k 0.0017083046453704408 1.9942633080314056 >5k 5.694348817901469E-4 2.417331274237752 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 6908 2.4244381115494225 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 2090 0.7335083458509399 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 1885 0.6615613549899625 RNA PCR Primer, Index 47 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 1724 0.6050566450942682 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 404 0.14178821613577977 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002105765586174947 0.0 2 0.0 0.0 0.0 0.00491345303440821 0.0 3 0.0 0.0 0.0 0.005966335827495683 0.0 4 0.0 0.0 0.0 0.027023991689245155 0.0 5 0.0 0.0 0.0 0.02877879634439094 0.0 6 0.0 0.0 0.0 0.029831679137478417 0.0 7 0.0 0.0 0.0 0.03088456193056589 0.0 8 0.0 0.0 0.0 0.03263936658571168 0.0 9 0.0 0.0 0.0 0.03439417124085747 0.0 10 0.0 0.0 0.0 0.03544705403394494 0.0 11 0.0 0.0 0.0 0.03649993682703241 0.0 12 0.0 0.0 0.0 0.03649993682703241 0.0 13 0.0 0.0 0.0 0.03685089775806157 0.0 14 0.0 0.0 0.0 0.03965858520629484 0.0 15 0.0 0.0 0.0 0.0424662726545281 0.0 16 0.0 0.0 0.0 0.04281723358555726 0.0 17 0.0 0.0 0.0 0.04597588196481968 0.0 18 0.0 0.0 0.0 0.04878356941305294 0.0 19 0.0 0.0 0.0 0.05369702244746115 0.0 20 0.0 0.0 0.0 0.05685567082672357 0.0 21 0.0 0.0 0.0 0.06001431920598599 0.0 22 0.0 0.0 0.0 0.0649277722403942 0.0 23 0.0 0.0 0.0 0.07089410806788989 0.0 24 0.0 0.0 0.0 0.08107197506773546 0.0 25 0.0 7.019218620583157E-4 0.0 0.09581233417096009 0.0 26 0.0 7.019218620583157E-4 0.0 0.1256440133084385 7.019218620583157E-4 27 0.0 7.019218620583157E-4 0.0 0.37061474316679066 7.019218620583157E-4 28 0.0 7.019218620583157E-4 0.0 0.613830668369997 7.019218620583157E-4 29 0.0 7.019218620583157E-4 0.0 0.8781042494349529 7.019218620583157E-4 30 0.0 0.0014038437241166313 0.0 1.15852203332725 7.019218620583157E-4 31 0.0 0.0014038437241166313 0.0 1.438588856288518 7.019218620583157E-4 32 0.0 0.0014038437241166313 0.0 1.7681411705248973 7.019218620583157E-4 33 0.0 0.0014038437241166313 0.0 2.1921019752081197 7.019218620583157E-4 34 0.0 0.0014038437241166313 0.0 2.7697836676821135 7.019218620583157E-4 35 0.0 0.0014038437241166313 0.0 3.528210239636124 7.019218620583157E-4 36 0.0 0.0014038437241166313 0.0 4.679011132480732 7.019218620583157E-4 37 0.0 0.0014038437241166313 0.0 6.2007777294231605 7.019218620583157E-4 38 0.0 0.0014038437241166313 0.0 7.8794238625356225 7.019218620583157E-4 39 0.0 0.0014038437241166313 0.0 9.552103659820588 0.0010528827930874735 40 0.0 0.0014038437241166313 0.0 11.141956677382673 0.0010528827930874735 41 0.0 0.0014038437241166313 0.0 12.7816461471509 0.0010528827930874735 42 0.0 0.0014038437241166313 0.0 14.390802015919588 0.0010528827930874735 43 0.0 0.0014038437241166313 0.0 15.92309744079289 0.0010528827930874735 44 0.0 0.0014038437241166313 0.0 17.350104586357446 0.0010528827930874735 45 0.0 0.0014038437241166313 0.0 18.85397217581739 0.0010528827930874735 46 0.0 0.0014038437241166313 0.0 20.272205298106215 0.0010528827930874735 47 0.0 0.0014038437241166313 0.0 21.671837491050496 0.0010528827930874735 48 0.0 0.0014038437241166313 0.0 22.99074866985807 0.0010528827930874735 49 0.0 0.0014038437241166313 0.0 24.285794505355664 0.0010528827930874735 50 0.0 0.0014038437241166313 0.0 25.567854786405178 0.0010528827930874735 51 0.0 0.0014038437241166313 0.0 26.764982522145633 0.0010528827930874735 52 0.0 0.0014038437241166313 0.0 27.957547765782714 0.0010528827930874735 53 0.0 0.0014038437241166313 0.0 29.15046397035082 0.0010528827930874735 54 0.0 0.0014038437241166313 0.0 30.227212106748276 0.0010528827930874735 55 0.0 0.0014038437241166313 0.0 31.295537180801034 0.0010528827930874735 56 0.0 0.0014038437241166313 0.0 32.31788637288897 0.0010528827930874735 57 0.0 0.0014038437241166313 0.0 33.34304325242514 0.0010528827930874735 58 0.0 0.0014038437241166313 0.0 34.32678674209987 0.0010528827930874735 59 0.0 0.0014038437241166313 0.0 35.29298218522314 0.0010528827930874735 60 0.0 0.0014038437241166313 0.0 36.25145648786377 0.0010528827930874735 61 0.0 0.0014038437241166313 0.0 37.229233641711005 0.0010528827930874735 62 0.0 0.0014038437241166313 0.0 38.16033299173136 0.0010528827930874735 63 0.0 0.0014038437241166313 0.0 39.10336501340671 0.0010528827930874735 64 0.0 0.0014038437241166313 0.0 40.00463268428958 0.0010528827930874735 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAGAT 15 0.0022231825 70.0 69 CTATTTG 15 0.0022231825 70.0 15 CTTTATA 40 1.0633812E-8 61.25 2 CTTAATA 30 8.363992E-6 58.333332 1 TTAATAC 25 2.387975E-4 56.000004 2 CATCGAT 25 2.387975E-4 56.000004 29 CTTTTCG 20 0.0069466983 52.5 48 AATATGT 20 0.0069466983 52.5 41 CATTAAG 20 0.0069466983 52.5 48 ACTTTTC 20 0.0069466983 52.5 47 TAGTAAC 20 0.0069466983 52.5 31 TCTTTAT 50 6.179471E-8 49.000004 1 TTTATAC 65 4.852973E-7 37.69231 3 TATGCCG 1640 0.0 33.292683 43 CTTGAAA 1600 0.0 33.250004 57 GTATGCC 1650 0.0 33.090908 42 TTGAAAA 1620 0.0 33.055553 58 CGTATGC 1655 0.0 32.990936 41 TCGTATG 1665 0.0 32.792793 40 CTCGTAT 1665 0.0 32.58258 39 >>END_MODULE