FastQCFastQC Report
Wed 25 May 2016
SRR1780173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448731
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAT407.077892E-427.469951
GTTTAGG508.404819E-526.3711551
TCGTTTA500.002585686321.9926625
CTCTAGC602.89448E-421.9759581
GTTATAC602.89448E-421.9759581
AGCTAGT500.002597636321.9752036
CGTTTAT550.00449269919.99332626
TGCTACG550.004512492619.9781482
TATGAAC550.004513393619.9774574
GCTAGTC550.004513393619.9774577
CAATCGA550.004513393619.9774574
ACACTAC600.007461169718.3126686
ATACACT902.2349229E-417.0918244
TAATACA1206.4773776E-616.4814014
TAGAACC953.375767E-416.1922534
AGCCAAT1004.987087E-415.3826426
TATACCA1452.313187E-615.1553135
AAGAGAC2509.094947E-1214.9431375
TAAGAGA2651.8189894E-1214.9265514
ACTTTCG900.004315366514.68917221
Produced by FastQC (version 0.11.5)