FastQCFastQC Report
Wed 25 May 2016
SRR1780173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448731
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT151761.0475374655474343No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC49230.3398146377760951No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG39370.2717550739233163No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG30810.21266888055822647RNA PCR Primer, Index 14 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1400.036.147342
CTATAAC250.002351516435.2043654
GGTATTT401.8213568E-532.99725713
GCTTATA300.00574190529.3369713
ATAGCAT454.0695286E-529.33393142
ATGCCGT24350.028.73159244
TCTTTAT1800.028.1690521
TATGCCG25250.027.35897443
TTTATAC1900.026.634883
TGGTATT609.761014E-625.66453412
GTCTTCT5600.024.35763743
TCTTCTG5650.024.14208444
TGCATTT551.5939836E-423.9996626
GTATTTT651.8148803E-523.6903414
TTTCGTC651.8148803E-523.6903418
CCGTCTT5700.023.54434241
TTAATAC703.212846E-522.0027282
CTCGTAT32150.021.41804939
TCGTATG32450.021.2885740
GTATGCC32600.021.19061742
Produced by FastQC (version 0.11.5)