FastQCFastQC Report
Wed 25 May 2016
SRR1780172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134312
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGC150.002220616770.067
GGGAGCT200.00693874352.533
AAAACGA200.00693874352.570
TATCGTG200.00693874352.511
GCATATA200.00693874352.51
TTAGAGG400.00241185935.04
ATGTAAC400.00241185935.048
TCTGCTA400.00241185935.010
TTGTGAT450.00429505631.11110962
GACATAG500.00718807127.9999981
TCAGTAA500.00718807127.99999812
TCCTAGA750.001824653523.33333456
TGGTCGC800.002655019821.87550
CCTATGA950.00717831318.42105314
AGATCTC1000.009647217.537
CGAAGTG2201.372573E-615.9090970
AAGTGTA1550.00732994313.54838770
CACGAAG3658.56744E-913.42465770
GCACGAA4455.4569682E-1113.37078770
ACGAAGT2751.38522755E-512.72727369
Produced by FastQC (version 0.11.5)