##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 134312 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.440623324795997 34.0 31.0 34.0 12.0 34.0 2 30.373391804157485 34.0 31.0 34.0 12.0 34.0 3 30.35904461254393 34.0 32.0 34.0 11.0 34.0 4 30.149941926261242 34.0 31.0 34.0 11.0 34.0 5 30.091220441956043 34.0 31.0 34.0 11.0 34.0 6 33.33647030793972 37.0 34.0 38.0 21.0 38.0 7 33.67181636785991 38.0 34.0 38.0 21.0 38.0 8 33.652324438620525 38.0 34.0 38.0 21.0 38.0 9 33.92312674965751 38.0 34.0 38.0 21.0 38.0 10-11 33.66104666746084 38.0 34.0 38.0 21.0 38.0 12-13 33.751436952766696 38.0 34.0 38.0 21.0 38.0 14-15 33.717713234856156 38.0 34.0 38.0 21.0 38.0 16-17 33.809797337542435 38.0 34.0 38.0 21.0 38.0 18-19 33.7958335815117 38.0 34.0 38.0 21.0 38.0 20-21 33.800777294657216 38.0 34.0 38.0 21.0 38.0 22-23 33.795881976294 38.0 34.0 38.0 21.0 38.0 24-25 33.80833060337126 38.0 34.0 38.0 21.0 38.0 26-27 33.82243582107332 38.0 34.0 38.0 21.0 38.0 28-29 33.92645482160938 38.0 35.0 38.0 21.0 38.0 30-31 33.951013312287806 38.0 35.0 38.0 21.0 38.0 32-33 34.00103862648163 38.0 35.0 38.0 21.0 38.0 34-35 33.95006775269522 38.0 35.0 38.0 21.0 38.0 36-37 33.88595583417714 38.0 34.0 38.0 21.0 38.0 38-39 33.88684927631187 38.0 34.5 38.0 21.0 38.0 40-41 33.95816829471678 38.0 35.0 38.0 21.0 38.0 42-43 34.03112529036869 38.0 35.0 38.0 21.0 38.0 44-45 34.05863214009173 38.0 35.0 38.0 21.0 38.0 46-47 34.01936908094586 38.0 35.0 38.0 21.0 38.0 48-49 33.961853743522546 38.0 35.0 38.0 21.0 38.0 50-51 33.911359372208 38.0 35.0 38.0 21.0 38.0 52-53 33.94771502174042 38.0 35.0 38.0 21.0 38.0 54-55 33.93178941568885 38.0 35.0 38.0 21.0 38.0 56-57 33.881198254809696 38.0 35.0 38.0 21.0 38.0 58-59 33.94915197450712 38.0 35.0 38.0 21.0 38.0 60-61 33.9636220144142 38.0 35.0 38.0 21.0 38.0 62-63 33.839228810530706 38.0 34.5 38.0 20.5 38.0 64-65 33.78746872952529 38.0 34.5 38.0 20.0 38.0 66-67 33.70833209244148 38.0 34.0 38.0 20.0 38.0 68-69 33.62747557924832 38.0 34.0 38.0 20.0 38.0 70-71 33.57513476085532 38.0 34.0 38.0 19.0 38.0 72-73 33.625230805884804 38.0 34.0 38.0 19.0 38.0 74-75 33.648683661921496 38.0 34.0 38.0 19.5 38.0 76 31.998317350646257 37.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2058620413083716 1101 2 0.36328203548459825 1101 3 0.20512499925524352 1101 4 0.395263973204127 1101 5 -0.049526439949186596 1101 6 -0.012349495833952062 1101 7 -0.2181913688283572 1101 8 -0.09566227619276191 1101 9 -0.14242230537028888 1101 10-11 0.042905350478804394 1101 12-13 0.6715464360396837 1101 14-15 0.3184898606289721 1101 16-17 -0.012961597710237527 1101 18-19 0.20812588733792126 1101 20-21 0.29194910501660587 1101 22-23 0.22491160037026248 1101 24-25 0.38167468149077166 1101 26-27 0.4256264958035558 1101 28-29 0.2435823201424867 1101 30-31 0.31738858601607944 1101 32-33 0.3124117064411962 1101 34-35 -0.07302239879816597 1101 36-37 -0.03261958909786955 1101 38-39 0.5129806587128627 1101 40-41 0.12385979014155879 1101 42-43 -0.026611874809830738 1101 44-45 0.33002205052446243 1101 46-47 0.41955167935950044 1101 48-49 0.1181913731337545 1101 50-51 0.37431177275302474 1101 52-53 0.39208742721103107 1101 54-55 0.613912419620462 1101 56-57 0.4451476680762383 1101 58-59 0.44460244184045905 1101 60-61 0.5080161392127707 1101 62-63 0.5534720318086457 1101 64-65 0.6455273122245302 1101 66-67 0.6320782679423473 1101 68-69 0.4338191575566199 1101 70-71 0.19722165757490018 1101 72-73 0.5617637146264016 1101 74-75 0.575400963566679 1101 76 -0.23504891674312134 1102 1 0.9619759012906819 1102 2 0.6848351979296154 1102 3 0.524242918934366 1102 4 1.1085625895817657 1102 5 0.09262769659293824 1102 6 0.3515128497514297 1102 7 0.046340036534232354 1102 8 0.0713245954050592 1102 9 0.5567367353631525 1102 10-11 0.4748323100487255 1102 12-13 0.8982262479614249 1102 14-15 0.6305506321543248 1102 16-17 0.5358991468504897 1102 18-19 0.6810980632146908 1102 20-21 0.6706588476729607 1102 22-23 0.6375630657291467 1102 24-25 0.25055918862796744 1102 26-27 0.29301530885443583 1102 28-29 0.027220690804973913 1102 30-31 0.3681115750448498 1102 32-33 0.5838780764969798 1102 34-35 0.4672433712946926 1102 36-37 0.31288153161938936 1102 38-39 -0.004643252169053369 1102 40-41 0.2608527287801792 1102 42-43 -0.04786758372669908 1102 44-45 0.20305658209835542 1102 46-47 0.06002895323584312 1102 48-49 0.10121629855971292 1102 50-51 0.37551675446763966 1102 52-53 0.4961961620644928 1102 54-55 0.05078570273217764 1102 56-57 0.24541141582216852 1102 58-59 -0.0659011225460091 1102 60-61 0.2179610302588344 1102 62-63 0.21443820805617975 1102 64-65 0.4836847856390989 1102 66-67 0.4086257846688355 1102 68-69 0.11957172340928679 1102 70-71 0.35152251538706025 1102 72-73 0.5991518535616294 1102 74-75 -0.055369045028292874 1102 76 0.39197975002466023 1103 1 0.07941708035837536 1103 2 -0.16493963289247304 1103 3 -0.27594652821710497 1103 4 -0.17144993319604396 1103 5 -0.1366756034801142 1103 6 -0.8338403166650465 1103 7 -0.28088175309915187 1103 8 -0.6844892647412024 1103 9 0.08450760063893625 1103 10-11 -0.3250962458983935 1103 12-13 -0.08545400377851564 1103 14-15 -0.8153003949542779 1103 16-17 -0.20949110625411294 1103 18-19 -0.41273045500354755 1103 20-21 -0.38158361257124085 1103 22-23 -0.7384694613834739 1103 24-25 -0.3430887126026363 1103 26-27 -0.47953973918968273 1103 28-29 -0.37052199822073106 1103 30-31 -0.7120181480893706 1103 32-33 -0.3029754150300832 1103 34-35 -0.3980765615960635 1103 36-37 0.0362445396548452 1103 38-39 -0.9856062216728745 1103 40-41 -0.4830363964716753 1103 42-43 -0.45301155553440253 1103 44-45 -0.4256711518407883 1103 46-47 -0.4605734244864621 1103 48-49 -0.4323022006335364 1103 50-51 -1.2250708527987797 1103 52-53 -0.43151289206441135 1103 54-55 -0.3030148800586332 1103 56-57 -0.3374396032590212 1103 58-59 -0.3996652608424398 1103 60-61 -0.5680479765082822 1103 62-63 -0.5874530524898489 1103 64-65 -0.2128064283478892 1103 66-67 -0.654911257868477 1103 68-69 -0.23929817616766513 1103 70-71 -0.1168614843560647 1103 72-73 -0.08806768839368573 1103 74-75 -0.7852744017267739 1103 76 -1.027894540551948 1104 1 -0.22154518857046668 1104 2 9.423644153372379E-5 1104 3 0.3996358977135124 1104 4 0.1794379498123142 1104 5 -0.050506636653068426 1104 6 0.5885609241554093 1104 7 -0.04039365484643298 1104 8 0.0659538854233972 1104 9 0.23887421243939144 1104 10-11 0.2510227082672145 1104 12-13 0.03607992886265521 1104 14-15 0.3016437681249755 1104 16-17 -0.0061168342865229874 1104 18-19 0.18608807500405788 1104 20-21 -0.20854930008706418 1104 22-23 0.039911932640336545 1104 24-25 0.4555549313578595 1104 26-27 -0.03185124210180845 1104 28-29 0.19342615321000522 1104 30-31 0.32506402461257267 1104 32-33 0.07348460649435395 1104 34-35 0.01630840928263666 1104 36-37 0.10169227958900251 1104 38-39 0.1913016980536355 1104 40-41 -0.015137086519793286 1104 42-43 0.3675601081779405 1104 44-45 0.3921980872070776 1104 46-47 0.5317426681952284 1104 48-49 0.28358710678606514 1104 50-51 0.471857958292631 1104 52-53 0.48229044422325984 1104 54-55 0.3005319039373191 1104 56-57 0.4093040343707628 1104 58-59 0.21366643705576394 1104 60-61 0.4787597596274509 1104 62-63 0.3495246633875908 1104 64-65 0.5182014217407414 1104 66-67 0.4969191105792419 1104 68-69 0.23896768227751153 1104 70-71 0.3068375533750043 1104 72-73 0.45792101127711504 1104 74-75 0.33178054889364006 1104 76 -0.03005489760436575 1105 1 0.2761693873564006 1105 2 0.1120324030235551 1105 3 -0.3346835537847106 1105 4 0.157964486125163 1105 5 0.14557957462169213 1105 6 0.1955632095794826 1105 7 0.16989710188287432 1105 8 -0.1145946163541538 1105 9 0.24625422767554994 1105 10-11 0.1993626016140979 1105 12-13 -0.7443606501114388 1105 14-15 0.052881706164598086 1105 16-17 0.0028344099999131345 1105 18-19 -0.07961627999848275 1105 20-21 -0.3107902853587703 1105 22-23 0.08135905046663794 1105 24-25 0.06438238184033906 1105 26-27 0.12743805449544254 1105 28-29 -0.033925929065254934 1105 30-31 0.12639084025502711 1105 32-33 0.10385376596617135 1105 34-35 0.36304215737812484 1105 36-37 -0.2562384593647806 1105 38-39 0.3002164390389055 1105 40-41 -0.12581350856143558 1105 42-43 -0.08974690045589284 1105 44-45 0.033981670244145334 1105 46-47 0.6133672238072165 1105 48-49 0.5367692423878978 1105 50-51 0.27385929145667376 1105 52-53 -0.06712391585800503 1105 54-55 0.06727580947312362 1105 56-57 0.11920595158762382 1105 58-59 0.2888394309613034 1105 60-61 0.2639457677533983 1105 62-63 0.29296041757753954 1105 64-65 -0.13606470319577113 1105 66-67 0.3700066550180381 1105 68-69 0.06331538415663829 1105 70-71 0.3003939271688125 1105 72-73 0.28890279136853536 1105 74-75 0.05359365199165467 1105 76 0.41872938375672675 1106 1 -0.05763334284043964 1106 2 -0.7148678847705874 1106 3 -0.25360101688703907 1106 4 0.06623202694730068 1106 5 -0.43196324822331533 1106 6 0.14658434013337285 1106 7 -0.4834032967197075 1106 8 -0.3734400539705405 1106 9 0.09657049343112334 1106 10-11 -0.30450415123691243 1106 12-13 0.07723006660369691 1106 14-15 -0.06388653490532192 1106 16-17 -0.21927110425897922 1106 18-19 -0.272288302406416 1106 20-21 0.10498375776418101 1106 22-23 -0.26875714449465704 1106 24-25 -0.0744898344823568 1106 26-27 -0.17428409334148398 1106 28-29 -0.08816819940707177 1106 30-31 -0.3941164161587807 1106 32-33 -0.23432358909793294 1106 34-35 -0.3661812601388519 1106 36-37 0.045433670773576296 1106 38-39 0.07417876416933922 1106 40-41 -0.08783853280078802 1106 42-43 -0.23867267419120708 1106 44-45 0.12330173734481775 1106 46-47 -0.11314369544113845 1106 48-49 -0.049961446932940135 1106 50-51 -0.29523054032996043 1106 52-53 0.009795609512508463 1106 54-55 -0.1191478481287973 1106 56-57 -0.1002947948303401 1106 58-59 -0.36092894861916136 1106 60-61 -0.23861282990423405 1106 62-63 -0.09365715479422221 1106 64-65 0.0987799341374398 1106 66-67 -0.5286921153160762 1106 68-69 -0.1383136538106413 1106 70-71 0.01947529442183793 1106 72-73 0.03501295342587696 1106 74-75 -0.5959054015195875 1106 76 0.36999675528819154 1107 1 -0.052963769989048615 1107 2 0.0389616190589166 1107 3 -0.08325621017859675 1107 4 -0.1540098523854887 1107 5 -0.653809583706014 1107 6 -0.38502798693350115 1107 7 -0.008612935565714963 1107 8 -0.4293008598313506 1107 9 0.33306127537645125 1107 10-11 -0.11020980921529855 1107 12-13 0.39103747132019606 1107 14-15 -0.007734353753143353 1107 16-17 0.13099605282636162 1107 18-19 0.1661861019770825 1107 20-21 0.06698766294989866 1107 22-23 0.3123738457272509 1107 24-25 0.026764971317902564 1107 26-27 0.11389363801806951 1107 28-29 0.3190193100531644 1107 30-31 -0.21545795340940543 1107 32-33 -0.05496506883031316 1107 34-35 0.2093341335583645 1107 36-37 0.2987296172513396 1107 38-39 0.12944168306862025 1107 40-41 0.6352906107829028 1107 42-43 0.3182968714146952 1107 44-45 0.41284931098330446 1107 46-47 0.10164151934408494 1107 48-49 0.10990141747537763 1107 50-51 0.10711332792300254 1107 52-53 0.02932153812935212 1107 54-55 0.15901716867258386 1107 56-57 0.4664187946105258 1107 58-59 0.17884188660621447 1107 60-61 0.04659817121585519 1107 62-63 0.11875543261836441 1107 64-65 0.2779416814810034 1107 66-67 -0.2417328624477335 1107 68-69 0.30828898597381027 1107 70-71 0.34910778314523583 1107 72-73 0.08287418310528238 1107 74-75 0.07166566377875228 1107 76 0.0422790184795474 1108 1 -0.6768743421754841 1108 2 -0.6513882609461348 1108 3 -0.498091375013761 1108 4 -0.369053429498031 1108 5 -0.1006960305924629 1108 6 -0.1505325972656948 1108 7 -0.579252066549671 1108 8 -0.43953200799353453 1108 9 -0.570784878469702 1108 10-11 -0.2767659542197194 1108 12-13 -0.4756102487758014 1108 14-15 -0.1302397219481648 1108 16-17 -0.14012937347148124 1108 18-19 -0.028237290722181285 1108 20-21 -0.27444532934516275 1108 22-23 0.3017163997249739 1108 24-25 -0.641591097900239 1108 26-27 -0.5333370049022292 1108 28-29 -0.035707859329171754 1108 30-31 -0.4845624121690406 1108 32-33 -0.5570176139863108 1108 34-35 -0.006451517572116927 1108 36-37 -0.44593376534307794 1108 38-39 -0.29431415879066947 1108 40-41 -0.49383026833797317 1108 42-43 -0.18135577478967235 1108 44-45 -0.7701745616957467 1108 46-47 -0.2560381573011128 1108 48-49 -0.27367190575311184 1108 50-51 -0.5403383583217902 1108 52-53 -0.4847778682114381 1108 54-55 -0.3685185140355216 1108 56-57 -0.6484447900806458 1108 58-59 -0.21624147830473817 1108 60-61 -0.3959463532252201 1108 62-63 -0.27403855500320873 1108 64-65 -0.35875782590815675 1108 66-67 -0.34276545054929386 1108 68-69 -0.46719415157828337 1108 70-71 -0.16527582175561406 1108 72-73 -0.6900511674062741 1108 74-75 -0.23078475936133458 1108 76 -0.6778801327140833 1109 1 0.20828835816448787 1109 2 0.34667714297162533 1109 3 0.4110635545637038 1109 4 0.45875133009400315 1109 5 0.3399142980460361 1109 6 0.7777254568058609 1109 7 0.2304290084058067 1109 8 0.1833674577665434 1109 9 0.303158783502127 1109 10-11 0.8637067671660645 1109 12-13 0.5312943974926156 1109 14-15 0.8318454326576301 1109 16-17 0.338537455086076 1109 18-19 0.3464794002281266 1109 20-21 0.4842644002970573 1109 22-23 0.972782451206271 1109 24-25 0.22310454723493933 1109 26-27 0.736494628576807 1109 28-29 0.614000737076843 1109 30-31 0.3277048696829965 1109 32-33 0.45640314042521624 1109 34-35 0.3650840174772654 1109 36-37 0.8634680478348429 1109 38-39 1.15466997590255 1109 40-41 0.41285987250709866 1109 42-43 0.5869743207259575 1109 44-45 0.6143931896398556 1109 46-47 0.3644491342643761 1109 48-49 0.6654733103712189 1109 50-51 0.7959656727612625 1109 52-53 0.3313181330836983 1109 54-55 0.37517178870043466 1109 56-57 0.7003531700941963 1109 58-59 0.5325564818701096 1109 60-61 0.9049521411895398 1109 62-63 0.9569190295847747 1109 64-65 0.7770362488854303 1109 66-67 0.8301097315779558 1109 68-69 0.723638777027837 1109 70-71 0.9761237249499146 1109 72-73 0.4212892891260296 1109 74-75 0.8774271558078439 1109 76 0.15397999356080305 1110 1 -0.5222542288338232 1110 2 -0.9031011927810546 1110 3 -0.36786853378813333 1110 4 -0.06281483570300495 1110 5 0.06388149958395317 1110 6 -0.14581803254248626 1110 7 -0.17781768624678307 1110 8 -0.04939177170498965 1110 9 -0.08540883390145382 1110 10-11 -0.29863385035521617 1110 12-13 -0.2442283770375724 1110 14-15 -0.04510540888185233 1110 16-17 -0.035396398733219314 1110 18-19 -0.4781900650313986 1110 20-21 -0.14760032566315573 1110 22-23 0.22949711243268212 1110 24-25 -0.11606921399539516 1110 26-27 -0.2539129982426118 1110 28-29 -0.02372534083911404 1110 30-31 0.0024665315763598983 1110 32-33 0.15802737048524307 1110 34-35 -0.27829940282168764 1110 36-37 -0.29523178346493495 1110 38-39 -0.1764140211157752 1110 40-41 -0.48860742711234906 1110 42-43 -0.4752956780970692 1110 44-45 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-0.4514753872127244 2116 12-13 -0.10748914173155555 2116 14-15 -0.12449259551138425 2116 16-17 -0.3281711765922637 2116 18-19 -0.08799616826664902 2116 20-21 -0.5663768775375431 2116 22-23 -0.5373560333322445 2116 24-25 -0.7582433164836857 2116 26-27 -0.820885967850927 2116 28-29 -0.6135348676127492 2116 30-31 -0.7213745228734609 2116 32-33 -0.49949818934108947 2116 34-35 -0.24449974206321912 2116 36-37 -0.8861267275212086 2116 38-39 -0.5042528347041042 2116 40-41 -0.856260435935738 2116 42-43 -0.6847189674949945 2116 44-45 -0.6894820169066875 2116 46-47 -0.4746216030084298 2116 48-49 -0.4157447240925549 2116 50-51 -0.09764908843159503 2116 52-53 -0.3199172119473843 2116 54-55 -0.22602027991235474 2116 56-57 -0.9464365065129314 2116 58-59 -0.47512704948562146 2116 60-61 -0.3673391484477193 2116 62-63 -0.535251872925258 2116 64-65 -0.6821540886706359 2116 66-67 -0.8995464176076595 2116 68-69 -0.3053996996422441 2116 70-71 -0.5082652907082803 2116 72-73 -0.5407061471789518 2116 74-75 -0.28299852677929493 2116 76 0.5137014507958355 2117 1 0.09136653738473655 2117 2 -0.573102403874671 2117 3 -0.5669319547239056 2117 4 -0.029348282071744336 2117 5 -0.08547863928811239 2117 6 0.7411465631540963 2117 7 0.088114250291909 2117 8 -0.3560487496227154 2117 9 -0.6236834291943367 2117 10-11 0.2824044605728844 2117 12-13 0.11182103558201817 2117 14-15 0.40304363637267215 2117 16-17 -0.22057664983329772 2117 18-19 -0.14876452710398524 2117 20-21 -0.18791359521222972 2117 22-23 0.08176387598683732 2117 24-25 0.24747686811675607 2117 26-27 -0.11672031324805232 2117 28-29 -0.4052480892142327 2117 30-31 -0.2102620623439435 2117 32-33 0.039134310051672117 2117 34-35 0.04026262535642644 2117 36-37 0.172694420563964 2117 38-39 0.20437399713136983 2117 40-41 0.347111760647536 2117 42-43 -0.09741024755329164 2117 44-45 0.004125788129343277 2117 46-47 -8.907310142518554E-4 2117 48-49 0.08504873392704582 2117 50-51 -0.09858423538325667 2117 52-53 -0.3581286927991414 2117 54-55 -0.027951297643710404 2117 56-57 -0.14698302096520166 2117 58-59 -0.24521449179798083 2117 60-61 -0.22615624827768954 2117 62-63 0.18104640251802806 2117 64-65 -0.2312715690365934 2117 66-67 -0.10306030312300152 2117 68-69 -0.11263393168822944 2117 70-71 -0.14357417258019467 2117 72-73 -1.4695863997900744 2117 74-75 -0.22902314017093062 2117 76 -0.4294043705951438 2118 1 -0.12495427947955662 2118 2 -0.18456485446755622 2118 3 -0.11740955683194443 2118 4 -0.4228347774604124 2118 5 -0.2323248184712483 2118 6 0.0629927423372294 2118 7 0.7493790724262936 2118 8 0.09152206724157708 2118 9 -0.19010306028788193 2118 10-11 -0.5998295644600518 2118 12-13 -0.3517041620464383 2118 14-15 -0.41568577732957124 2118 16-17 -0.10696910701379636 2118 18-19 -0.30396874317776934 2118 20-21 -0.20002657281433045 2118 22-23 -0.05525852190511671 2118 24-25 -0.29586552318759374 2118 26-27 -0.19520351482908893 2118 28-29 0.19342615321000522 2118 30-31 -0.18345870266015396 2118 32-33 -0.2461176662329123 2118 34-35 -0.5959074998082698 2118 36-37 -0.6156372658655442 2118 38-39 -0.5189255746736379 2118 40-41 -0.3262166319743329 2118 42-43 -0.1267580736402465 2118 44-45 -0.24984736733837565 2118 46-47 -0.34751869544113134 2118 48-49 -0.28175380230484137 2118 50-51 -0.44859658716191575 2118 52-53 -0.6640163739585603 2118 54-55 -0.2719112778808679 2118 56-57 -0.6191050565383236 2118 58-59 -0.44258356294423606 2118 60-61 -0.8777743312816426 2118 62-63 -0.3933730638851358 2118 64-65 -0.6224235782592586 2118 66-67 -0.6749558894207581 2118 68-69 -0.5735323177224885 2118 70-71 -0.22583290117044896 2118 72-73 -0.3474767159956116 2118 74-75 -0.2733330874700002 2118 76 -0.1067594430589125 2119 1 -0.012087499285687642 2119 2 -0.32669217959200125 2119 3 -0.3691288389907079 2119 4 -0.3179503787179456 2119 5 0.326057091405616 2119 6 -0.3113113577413671 2119 7 -0.26778100092974455 2119 8 -0.3551883728946521 2119 9 -0.4807272415820947 2119 10-11 -0.558308900325919 2119 12-13 -0.3769898903671063 2119 14-15 -0.040919108479684496 2119 16-17 -0.050556184561607154 2119 18-19 -0.2454149050850205 2119 20-21 -0.6823670889264619 2119 22-23 -0.6722352051642275 2119 24-25 -0.2811410995564714 2119 26-27 -0.032633106131157774 2119 28-29 0.13673077726031124 2119 30-31 -0.1823207016122126 2119 32-33 -0.29220261514567625 2119 34-35 -0.3987508650165452 2119 36-37 -0.6021040591587052 2119 38-39 -0.5939804279615544 2119 40-41 -0.35586196862615793 2119 42-43 -0.5348992840148838 2119 44-45 -0.4223036484492013 2119 46-47 -1.1618730640552286 2119 48-49 -0.6149220299705505 2119 50-51 -0.3391806518724181 2119 52-53 -0.5513542702121583 2119 54-55 -0.5644880724839609 2119 56-57 -0.43614478014325186 2119 58-59 -0.36129017153736953 2119 60-61 -0.6465922838622049 2119 62-63 -0.7019883049121276 2119 64-65 -0.66184835557128 2119 66-67 -0.8190399213830375 2119 68-69 -1.1297078154956068 2119 70-71 -1.0173535653045818 2119 72-73 -0.3057472818850542 2119 74-75 -0.4833229355182098 2119 76 -0.1930346591972416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 15.0 13 240.0 14 892.0 15 1502.0 16 1964.0 17 2404.0 18 2416.0 19 2240.0 20 1738.0 21 1392.0 22 1080.0 23 927.0 24 975.0 25 962.0 26 1136.0 27 1372.0 28 1631.0 29 1993.0 30 2385.0 31 3040.0 32 3723.0 33 4990.0 34 6763.0 35 9888.0 36 18509.0 37 60134.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.38778366787778 17.68717612722616 9.028232771457501 29.89680743343856 2 21.94740603966883 20.49854071117994 28.260319256656143 29.293733992495085 3 19.53883495145631 21.403895407707427 29.326493537435226 29.730776103401034 4 17.782476621597475 18.72654714396331 31.05679313836441 32.43418309607481 5 16.931472988266126 29.660045267734823 29.715885401155518 23.692596342843526 6 27.278277443564235 33.18988623503484 19.19262612424802 20.339210197152898 7 27.688722442688984 29.448072011972215 20.04452353120742 22.81868201413138 8 25.506283876347606 28.677258919530647 21.292215140866045 24.524242063255702 9 23.67993924593484 18.238876645422597 21.35996783608315 36.72121627255942 10-11 25.894931204955622 22.76006611471797 25.687205908630652 25.65779677169575 12-13 23.295759128000476 20.504869259634283 26.57580856513193 29.623563047233308 14-15 21.1838852820299 21.90943474894276 25.049883852522488 31.856796116504853 16-17 24.761376496515574 24.645229019000535 24.509351361010186 26.084043123473705 18-19 24.2804972023989 23.601478652237525 24.646437572359776 27.471586573003805 20-21 23.977381023289055 24.118098159509202 24.98883197331586 26.915688843885878 22-23 24.97282446840193 23.79943713145512 24.46132884626839 26.76640955387456 24-25 24.3436922985288 24.861516469116683 23.740618857585325 27.05417237476919 26-27 23.609952945380904 25.112424801953658 24.68208350705819 26.595538745607243 28-29 24.124798975519685 24.5506730597415 25.598606230269823 25.725921734468997 30-31 24.1583030555721 24.0641193638692 25.731133480254933 26.046444100303773 32-33 23.305065816903927 24.879013044255167 25.675293346834238 26.140627792006672 34-35 23.47407528739055 24.44718267913515 25.79218535946155 26.286556674012747 36-37 23.704508904639944 23.743596998034427 26.01182321758294 26.54007087974269 38-39 22.798037405444042 23.567514443981178 27.9312347370302 25.703213413544585 40-41 22.700875573292038 23.234334981237716 27.730582524271846 26.334206921198405 42-43 21.383420692119838 23.42419143486807 28.051477157662756 27.140910715349335 44-45 20.789653940079813 23.063836440526536 29.35776401215081 26.788745607242838 46-47 20.924492690499324 23.709064376468135 28.910033764793035 26.456409168239503 48-49 20.431904818631246 24.187712192507 28.78112156769313 26.599261421168624 50-51 20.294538090416346 24.53168741437846 28.122952528441242 27.050821966763955 52-53 19.888095956042484 24.74992833822867 28.01435469040253 27.34762101532631 54-55 19.70538526256226 24.36025344163918 28.235215283930582 27.699146011867974 56-57 20.168414469349237 24.52433335939216 27.739620211225397 27.567631960033207 58-59 20.41999225683483 24.54657811662398 27.219086902138308 27.814342724402884 60-61 20.36601346119483 23.803904342128774 27.260780868425755 28.56930132825064 62-63 20.540979212579664 23.928613973434988 26.98790874977664 28.54249806420871 64-65 20.94265590565251 23.984826374411817 26.24188456727619 28.83063315265948 66-67 20.800449699207814 24.987715170647448 25.189484186074218 29.022350944070524 68-69 21.306440624965102 24.903303142322137 25.034714078839116 28.75554215387364 70-71 21.63767943296206 25.09753409970814 25.167520400262077 28.09726606706772 72-73 21.66113228899875 24.84588123175889 24.80195366013461 28.691032819107747 74-75 21.5714961116509 25.38948638054076 24.51428209795885 28.524735409849495 76 21.683257514276566 25.6516592088511 23.962296461198264 28.70278681567407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 4.0 14 8.5 15 10.0 16 16.5 17 24.0 18 30.5 19 62.0 20 108.5 21 130.0 22 151.0 23 231.5 24 350.0 25 409.0 26 476.5 27 629.0 28 836.5 29 959.0 30 1117.0 31 1448.0 32 1783.0 33 1945.0 34 2175.0 35 2701.5 36 3301.0 37 3604.0 38 3907.5 39 4515.0 40 5339.5 41 6017.5 42 6175.0 43 6310.0 44 6674.0 45 6881.5 46 6860.0 47 6802.5 48 6653.5 49 6315.5 50 6069.0 51 5676.0 52 4892.0 53 4264.5 54 4028.0 55 3853.5 56 3516.5 57 3226.5 58 3099.0 59 2956.5 60 2848.0 61 2936.0 62 2990.0 63 2973.0 64 3014.5 65 3011.0 66 2791.5 67 2634.0 68 2565.5 69 2422.0 70 2248.5 71 2150.0 72 1954.5 73 1556.5 74 1153.5 75 953.0 76 755.5 77 432.0 78 207.0 79 108.0 80 71.0 81 26.5 82 15.5 83 12.0 84 8.5 85 5.5 86 4.0 87 2.0 88 2.5 89 3.5 90 4.5 91 5.0 92 5.0 93 5.5 94 3.5 95 2.5 96 4.0 97 4.5 98 4.5 99 17.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 7.445351122758949E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.7226755613794745E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.7226755613794745E-4 48-49 0.0 50-51 0.0 52-53 3.7226755613794745E-4 54-55 7.445351122758949E-4 56-57 3.7226755613794745E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.7226755613794745E-4 70-71 0.0 72-73 0.0 74-75 3.7226755613794745E-4 76 7.445351122758949E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 134312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.7019774852582 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26984557030615 52.39889213175294 2 14.634516391221892 20.108404312347368 3 4.7878623679219725 9.868068378104713 4 1.8390680032511515 5.053904342128774 5 0.8247087510159848 2.8329561022097804 6 0.4952587374695204 2.041515277860504 7 0.33595231644540774 1.6156411936386919 8 0.21024112706583584 1.155518494252189 9 0.1625575724735844 1.0051224015724582 >10 0.43998916282850176 3.9199773661325867 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005211745785931265 0.0 2 0.0 0.0 0.0 0.005956280898207159 0.0 3 0.0 0.0 0.0 0.006700816010483055 0.0 4 0.0 0.0 0.0 0.011168026684138424 0.0 5 0.0 0.0 0.0 0.01637977247006969 0.0 6 0.0 0.0 0.0 0.01637977247006969 0.0 7 0.0 0.0 0.0 0.01637977247006969 0.0 8 0.0 0.0 0.0 0.017124307582345583 0.0 9 0.0 0.0 0.0 0.020102448031449165 0.0 10 0.0 0.0 0.0 0.02084698314372506 0.0 11 0.0 0.0 0.0 0.021591518256000954 0.0 12 0.0 0.0 0.0 0.021591518256000954 0.0 13 0.0 0.0 0.0 0.023825123592828637 0.0 14 0.0 0.0014890702245517898 0.0 0.025314193817380426 0.0 15 0.0 0.0014890702245517898 0.0 0.025314193817380426 0.0 16 0.0 0.0014890702245517898 0.0 0.025314193817380426 0.0 17 0.0 0.0014890702245517898 0.0 0.028292334266484007 0.0 18 0.0 0.0014890702245517898 0.0 0.029781404491035796 0.0 19 0.0 0.0014890702245517898 0.0 0.03201500982786348 0.0 20 0.0 0.0014890702245517898 0.0 0.03424861516469117 0.0 21 0.0 0.0014890702245517898 0.0 0.039460360950622435 0.0 22 0.0 0.0014890702245517898 0.0 0.0439275716242778 0.0 23 0.0 0.0014890702245517898 0.0 0.046161176961105485 0.0 24 0.0 0.0014890702245517898 0.0 0.05584013342069212 0.0 25 0.0 0.0014890702245517898 0.0 0.06775269521710645 0.0 26 0.0 0.0014890702245517898 0.0 0.10870212639228066 0.0 27 0.0 0.0014890702245517898 0.0 0.18241110250759426 0.0 28 0.0 0.0014890702245517898 0.0 0.29781404491035796 0.0 29 0.0 0.0014890702245517898 0.0 0.40130442551670736 0.0 30 0.0 0.0014890702245517898 0.0 0.5420215617368516 0.0 31 0.0 0.0014890702245517898 0.0 0.7028411459884448 0.0 32 0.0 0.0014890702245517898 0.0 0.8971648102924534 0.0 33 0.0 0.0014890702245517898 0.0 1.1458395377926023 0.0 34 0.0 0.0014890702245517898 0.0 1.5612901304425517 0.0 35 0.0 0.0014890702245517898 0.0 2.062362261004229 0.0 36 0.0 0.0014890702245517898 0.0 2.698939782000119 0.0 37 0.0 0.0014890702245517898 0.0 3.5328191077491216 0.0 38 0.0 0.0014890702245517898 0.0 4.374143784620883 0.0 39 0.0 0.0014890702245517898 0.0 5.229614628625886 0.0 40 0.0 0.0014890702245517898 0.0 6.081362797069509 0.0 41 0.0 0.0014890702245517898 0.0 6.936089105962237 0.0 42 0.0 0.0014890702245517898 0.0 7.754333194353446 0.0 43 0.0 0.0014890702245517898 0.0 8.647030793972244 0.0 44 0.0 0.0014890702245517898 0.0 9.535261182917386 0.0 45 0.0 0.0014890702245517898 0.0 10.436893203883495 0.0 46 0.0 0.0014890702245517898 0.0 11.390642682708917 0.0 47 0.0 0.0014890702245517898 0.0 12.335457740187028 0.0 48 0.0 0.0014890702245517898 0.0 13.24155697182679 0.0 49 0.0 0.0014890702245517898 0.0 14.058311989993449 0.0 50 0.0 0.0014890702245517898 0.0 14.890702245517899 0.0 51 0.0 0.0014890702245517898 0.0 15.670975043183036 0.0 52 0.0 0.0014890702245517898 0.0 16.39913038298886 0.0 53 0.0 0.0014890702245517898 0.0 17.081124545833582 0.0 54 0.0 0.0014890702245517898 0.0 17.809279885639405 0.0 55 0.0 0.0014890702245517898 0.0 18.598487104651856 0.0 56 0.0 0.0014890702245517898 0.0 19.32515337423313 0.0 57 0.0 0.0014890702245517898 0.0 20.096491750550957 0.0 58 0.0 0.0014890702245517898 0.0 20.802311036988506 0.0 59 0.0 0.0014890702245517898 0.0 21.48728334028233 0.0 60 0.0 0.0014890702245517898 0.0 22.15662040621836 0.0 61 0.0 0.0014890702245517898 0.0 22.844570849961283 0.0 62 0.0 0.0014890702245517898 0.0 23.477425695395794 0.0 63 0.0 0.0014890702245517898 0.0 24.175799630710586 0.0 64 0.0 0.0014890702245517898 0.0 24.860027398892132 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGC 15 0.0022206167 70.0 67 GGGAGCT 20 0.006938743 52.5 33 AAAACGA 20 0.006938743 52.5 70 TATCGTG 20 0.006938743 52.5 11 GCATATA 20 0.006938743 52.5 1 TTAGAGG 40 0.002411859 35.0 4 ATGTAAC 40 0.002411859 35.0 48 TCTGCTA 40 0.002411859 35.0 10 TTGTGAT 45 0.004295056 31.111109 62 GACATAG 50 0.007188071 27.999998 1 TCAGTAA 50 0.007188071 27.999998 12 TCCTAGA 75 0.0018246535 23.333334 56 TGGTCGC 80 0.0026550198 21.875 50 CCTATGA 95 0.007178313 18.421053 14 AGATCTC 100 0.0096472 17.5 37 CGAAGTG 220 1.372573E-6 15.90909 70 AAGTGTA 155 0.007329943 13.548387 70 CACGAAG 365 8.56744E-9 13.424657 70 GCACGAA 445 5.4569682E-11 13.370787 70 ACGAAGT 275 1.38522755E-5 12.727273 69 >>END_MODULE