FastQCFastQC Report
Wed 25 May 2016
SRR1780172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134312
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT69745.192387873012091No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG23871.7772053130025611No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG14201.057239859431771RNA PCR Primer, Index 18 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC13370.9954434451128715No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC4350.3238727738400143No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT2630.19581273452856035No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2480.18464470784442194RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1910.14220620644469592No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACGT150.002220616770.023
CCGGGGG200.00693874352.514
TGAGTCG200.00693874352.566
GGGCATA200.00693874352.539
TGAATTA200.00693874352.519
GATTATC200.00693874352.550
TCGAAAT200.00693874352.570
AACTCGT552.2610038E-950.9090937
TCTTTAT851.8189894E-1245.294121
CCTTTAA551.3010504E-744.5454521
ACACAAG404.5730583E-543.7527
CGGAACT659.911673E-943.07692734
CCGGAAC659.911673E-943.07692733
ATCGTAT503.4578989E-642.039
CGTCAGA350.001251813640.02
ACTCGTA801.4060788E-939.37538
CCTTATC459.158561E-538.88888517
CTCTCCA459.158561E-538.8888859
CTTTATA1007.2759576E-1238.52
GCCGGAA852.5429472E-937.05882632