##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780172_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 134312 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.7288998749181 34.0 34.0 34.0 34.0 34.0 2 33.712750908332836 34.0 34.0 34.0 34.0 34.0 3 33.734156292810766 34.0 34.0 34.0 34.0 34.0 4 33.7595523854905 34.0 34.0 34.0 34.0 34.0 5 33.74462445648937 34.0 34.0 34.0 34.0 34.0 6 37.49158675323128 38.0 38.0 38.0 37.0 38.0 7 37.4887128476979 38.0 38.0 38.0 37.0 38.0 8 37.38837185061647 38.0 38.0 38.0 37.0 38.0 9 37.40886145690631 38.0 38.0 38.0 37.0 38.0 10-11 37.36147179700995 38.0 38.0 38.0 37.0 38.0 12-13 37.35624143784621 38.0 38.0 38.0 37.0 38.0 14-15 37.272764161057836 38.0 38.0 38.0 36.0 38.0 16-17 37.303669813568405 38.0 38.0 38.0 36.5 38.0 18-19 37.25766871165644 38.0 38.0 38.0 36.0 38.0 20-21 37.27873160998273 38.0 38.0 38.0 36.0 38.0 22-23 37.273378402525466 38.0 38.0 38.0 36.0 38.0 24-25 37.29943340877956 38.0 38.0 38.0 36.0 38.0 26-27 37.17510349038061 38.0 38.0 38.0 36.0 38.0 28-29 37.14203124069331 38.0 38.0 38.0 36.0 38.0 30-31 37.17110905950325 38.0 38.0 38.0 36.0 38.0 32-33 37.06050836857466 38.0 38.0 38.0 36.0 38.0 34-35 37.10609253082375 38.0 38.0 38.0 36.0 38.0 36-37 37.06430177497171 38.0 38.0 38.0 36.0 38.0 38-39 36.33404684614926 38.0 37.0 38.0 34.0 38.0 40-41 36.7533839120853 38.0 37.0 38.0 34.5 38.0 42-43 37.01075480969683 38.0 38.0 38.0 35.5 38.0 44-45 37.09403106200489 38.0 38.0 38.0 36.0 38.0 46-47 37.03730493180058 38.0 38.0 38.0 36.0 38.0 48-49 37.05385594734648 38.0 38.0 38.0 36.0 38.0 50-51 36.904375632854844 38.0 38.0 38.0 35.0 38.0 52-53 36.915137887902794 38.0 38.0 38.0 35.0 38.0 54-55 36.88673759604503 38.0 37.5 38.0 35.5 38.0 56-57 36.942421377092145 38.0 38.0 38.0 35.5 38.0 58-59 36.83345121805944 38.0 37.5 38.0 35.0 38.0 60-61 36.842255345762105 38.0 37.0 38.0 35.0 38.0 62-63 36.827089165525045 38.0 37.0 38.0 35.0 38.0 64-65 36.80406441717791 38.0 37.5 38.0 34.5 38.0 66-67 36.538354726308896 38.0 37.0 38.0 34.0 38.0 68-69 36.47077699684317 38.0 37.0 38.0 34.0 38.0 70-71 35.898121537911734 38.0 37.0 38.0 31.5 38.0 72-73 34.956478200011915 38.0 37.0 38.0 24.0 38.0 74-75 34.69180341294896 38.0 37.0 38.0 23.0 38.0 76 34.00067008160105 38.0 35.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09863830775798021 1101 2 0.007342496919264363 1101 3 0.018174138728177525 1101 4 0.07671302637088218 1101 5 0.0266192086217103 1101 6 -0.01973381026324006 1101 7 0.04781753519311849 1101 8 0.03212188195562504 1101 9 0.05300120689313559 1101 10-11 0.0561055628151621 1101 12-13 0.0550410638050991 1101 14-15 0.24017336495040809 1101 16-17 0.28621478644893017 1101 18-19 -3.286434364326851E-4 1101 20-21 0.14710223228820496 1101 22-23 0.1695801261185821 1101 24-25 0.060569089440861035 1101 26-27 -0.00763030610978177 1101 28-29 -0.02381254637958108 1101 30-31 0.15484394766252763 1101 32-33 0.007881357652905763 1101 34-35 0.23925737684237447 1101 36-37 -0.0959767038169943 1101 38-39 0.4643831323577601 1101 40-41 0.3717380899011147 1101 42-43 0.33524707631570294 1101 44-45 0.09534880528403278 1101 46-47 0.26931221160485563 1101 48-49 0.34359846212042555 1101 50-51 0.04418328654117687 1101 52-53 0.1569317076667005 1101 54-55 0.13017948753332576 1101 56-57 -0.0255036455783042 1101 58-59 0.04950849685064185 1101 60-61 0.042465895546591526 1101 62-63 -0.008850877782229816 1101 64-65 0.16154437025821267 1101 66-67 -0.08744987228519818 1101 68-69 -0.5171748289695728 1101 70-71 -0.13487232763706913 1101 72-73 0.20459466872781462 1101 74-75 0.3640794369596634 1101 76 -0.5813750501610571 1102 1 0.034588895081235194 1102 2 0.04300321451536604 1102 3 0.13965820431963039 1102 4 0.04436390187857597 1102 5 -0.02184127977507444 1102 6 0.12617852099718618 1102 7 0.11516168808254434 1102 8 -0.01971761110940662 1102 9 -0.23603430214843968 1102 10-11 -0.01325672378509779 1102 12-13 0.1703064125756839 1102 14-15 0.2700535408945939 1102 16-17 -0.09530517580058984 1102 18-19 0.09697573662997172 1102 20-21 0.10537026066847233 1102 22-23 0.059577092598182446 1102 24-25 0.022203482821048226 1102 26-27 0.2606087352977724 1102 28-29 0.11821519347918752 1102 30-31 0.05235309035595748 1102 32-33 0.21766614623338398 1102 34-35 0.24004946272470562 1102 36-37 0.20862666339065328 1102 38-39 0.17344745984313903 1102 40-41 0.47649144783967756 1102 42-43 0.18616218830316456 1102 44-45 0.30595095908351766 1102 46-47 0.39594904865425207 1102 48-49 0.24024389414428526 1102 50-51 0.3436970806325448 1102 52-53 0.08821825737430089 1102 54-55 0.1546751647667577 1102 56-57 0.1302128550873789 1102 58-59 0.3106019124260868 1102 60-61 0.39137130026878 1102 62-63 0.4433922420249701 1102 64-65 0.32533761899003366 1102 66-67 0.39338839051878693 1102 68-69 0.5929461747886151 1102 70-71 0.23514553642750968 1102 72-73 0.1916769276893362 1102 74-75 0.4590539048296236 1102 76 0.16272222472644415 1103 1 -0.2060009598632604 1103 2 0.03707094989943727 1103 3 0.040254641749889686 1103 4 0.028341168220393342 1103 5 0.13532760914949193 1103 6 -0.18144030491211538 1103 7 -0.22501636013014803 1103 8 -0.1037004966464039 1103 9 -0.20829153284851287 1103 10-11 -0.08187111053022278 1103 12-13 -0.15752144739397522 1103 14-15 0.1428269348081983 1103 16-17 0.02070712220940152 1103 18-19 0.16067332760072617 1103 20-21 -0.06246707165875165 1103 22-23 0.0821662571844115 1103 24-25 -0.06941348072643194 1103 26-27 0.0992455185493597 1103 28-29 -0.04173921076571219 1103 30-31 -0.03406611765861811 1103 32-33 0.007335657283135788 1103 34-35 -0.08905877100170301 1103 36-37 0.11619893025425654 1103 38-39 -0.34883666271953473 1103 40-41 0.21794425061395373 1103 42-43 -0.2661188035650568 1103 44-45 -0.005296261421726456 1103 46-47 -0.2983587066378277 1103 48-49 -0.1072029843927993 1103 50-51 -0.10214993276522222 1103 52-53 -0.032951659060238114 1103 54-55 -0.08012691915190828 1103 56-57 -0.08033838795886794 1103 58-59 0.10532468541862983 1103 60-61 0.053436916413524216 1103 62-63 0.09325617722762303 1103 64-65 -0.006580212321722456 1103 66-67 -0.3585000604296553 1103 68-69 -0.4023537698221702 1103 70-71 -0.3667746228365303 1103 72-73 -0.5297795676989097 1103 74-75 -0.9594557240626713 1103 76 -1.1165116381491345 1104 1 0.07463179526462227 1104 2 0.1515144799070356 1104 3 0.00485010895678073 1104 4 0.1407746956957041 1104 5 -0.020629595206038687 1104 6 0.09180925193771827 1104 7 -0.06088461791693334 1104 8 -0.2916753003941892 1104 9 0.031436879407756635 1104 10-11 0.15798787275668502 1104 12-13 -0.014577822425628995 1104 14-15 0.013573484567629635 1104 16-17 0.1306044063319689 1104 18-19 0.12617487862630128 1104 20-21 0.17135794733339793 1104 22-23 0.06633550889370099 1104 24-25 0.10992780820748038 1104 26-27 0.18884098575624364 1104 28-29 0.22637850891015177 1104 30-31 0.19425117785922907 1104 32-33 0.1828547760473782 1104 34-35 0.22945540980356327 1104 36-37 0.001735616757173375 1104 38-39 0.17060655075223252 1104 40-41 0.05980294901861072 1104 42-43 0.02000584824552476 1104 44-45 0.1696610062409718 1104 46-47 0.057340521014744184 1104 48-49 0.14692780821292928 1104 50-51 0.01396332375541931 1104 52-53 0.02145689373794113 1104 54-55 -0.16669551246667424 1104 56-57 -0.20286940847038437 1104 58-59 0.14098244687722428 1104 60-61 0.1067850741749865 1104 62-63 -0.05897381770780186 1104 64-65 -0.08512462229893458 1104 66-67 -0.288581251870518 1104 68-69 -0.08273171360541198 1104 70-71 0.1330038020811628 1104 72-73 0.3382170770212767 1104 74-75 0.24877341622600824 1104 76 0.2237485543046489 1105 1 -0.25197752418687713 1105 2 0.10342534836818373 1105 3 0.06481074887551586 1105 4 0.03894635643720079 1105 5 0.10983002896866623 1105 6 0.2244590741825121 1105 7 -0.1907887570738609 1105 8 -0.03313670212094877 1105 9 0.043657549798815865 1105 10-11 0.08649695096897148 1105 12-13 0.11814341932144856 1105 14-15 0.07698606709683276 1105 16-17 0.02437186443252415 1105 18-19 0.16460650635610818 1105 20-21 0.2062106644486832 1105 22-23 -0.041166713046365544 1105 24-25 -0.16792579768383575 1105 26-27 0.07771224020012113 1105 28-29 0.19464444339466525 1105 30-31 0.09616839472058558 1105 32-33 0.09823060133940231 1105 34-35 0.029702997411483523 1105 36-37 0.15578748318682756 1105 38-39 0.13015137045766068 1105 40-41 0.2960904045798074 1105 42-43 0.08233126723993678 1105 44-45 -0.26623602270773716 1105 46-47 -0.007175096049756746 1105 48-49 0.04062384681120079 1105 50-51 0.15418361324247343 1105 52-53 0.2837489836312841 1105 54-55 0.1343694316674089 1105 56-57 0.17479691961493415 1105 58-59 0.23224069979749373 1105 60-61 0.2808026592435411 1105 62-63 0.15028532398849137 1105 64-65 -0.06450025713895968 1105 66-67 -0.22956493648199938 1105 68-69 0.03220607207259718 1105 70-71 0.07453503621016466 1105 72-73 0.4181593065794189 1105 74-75 0.2910616970792361 1105 76 0.24612666502074632 1106 1 -0.060440518784965036 1106 2 -0.10347863304062344 1106 3 -0.09518432630493123 1106 4 -0.04930794893239465 1106 5 0.01277260865070673 1106 6 -0.15732986651958214 1106 7 0.16062020301971103 1106 8 0.1881800715066433 1106 9 0.09255946893943445 1106 10-11 0.03767967716439813 1106 12-13 -0.03149414473967482 1106 14-15 -0.03683112975743086 1106 16-17 -0.06399786639529736 1106 18-19 -0.0366608651753495 1106 20-21 -0.024578691785059448 1106 22-23 -0.202646929122821 1106 24-25 -0.07886351410657255 1106 26-27 -0.10093174151648299 1106 28-29 0.08360130505340635 1106 30-31 -0.04507389101129178 1106 32-33 0.10055450056528059 1106 34-35 -0.07960106402563838 1106 36-37 -0.07854158709957915 1106 38-39 0.04259346535278041 1106 40-41 -0.148186031697648 1106 42-43 -0.08975654844869041 1106 44-45 0.02684075830708821 1106 46-47 -0.1894742172772652 1106 48-49 -0.06226640666310601 1106 50-51 -0.022451965500778215 1106 52-53 -0.141809290835063 1106 54-55 -0.0020519174253479378 1106 56-57 0.08673114249379665 1106 58-59 -0.12537430243406789 1106 60-61 -0.1906064547506361 1106 62-63 -0.13860536040752436 1106 64-65 -0.1472372256047194 1106 66-67 -0.18394110035537636 1106 68-69 -0.016099482200452542 1106 70-71 0.05208093706739447 1106 72-73 -0.2982326474966328 1106 74-75 0.005445248181935369 1106 76 -0.673806542114086 1107 1 0.028334342717172945 1107 2 -0.05022128432872819 1107 3 0.05411334572001891 1107 4 0.0064090613300749055 1107 5 -0.06071451029095698 1107 6 -0.0236378010093361 1107 7 -0.05267407845639127 1107 8 0.09325227203338926 1107 9 0.24503578050665453 1107 10-11 -0.03620855581474558 1107 12-13 0.1173683563955521 1107 14-15 0.07786856448771573 1107 16-17 0.1716414942440565 1107 18-19 -0.08531675474033307 1107 20-21 0.2921121930876396 1107 22-23 0.11449047892367048 1107 24-25 0.07711686914653626 1107 26-27 -0.07645621704096328 1107 28-29 -0.04346102655438244 1107 30-31 -0.0028017691937165523 1107 32-33 0.23342920162180292 1107 34-35 -0.1043982365500824 1107 36-37 0.23333214522870094 1107 38-39 0.33416174555743083 1107 40-41 0.2677200319356956 1107 42-43 0.24012947461914536 1107 44-45 0.10481959764956628 1107 46-47 0.021095516019741467 1107 48-49 0.157604631389745 1107 50-51 0.21972631076840088 1107 52-53 0.1404160595721038 1107 54-55 0.22908058643442075 1107 56-57 -0.08636091696189396 1107 58-59 0.27516076855554417 1107 60-61 0.036995488760403816 1107 62-63 0.2770963621123741 1107 64-65 0.25214748059816827 1107 66-67 0.21898930257503224 1107 68-69 0.17357448020078436 1107 70-71 -0.14272047364310936 1107 72-73 0.20837816593236624 1107 74-75 -0.010077108249511468 1107 76 0.21282198087807558 1108 1 -0.12570127683569154 1108 2 -0.09136692030195093 1108 3 0.09112231376346358 1108 4 0.02766079809904909 1108 5 0.0048406242296934465 1108 6 -0.04514215872058713 1108 7 0.07035232043207884 1108 8 0.06549554600079688 1108 9 0.1973851552698278 1108 10-11 -0.17372908126630193 1108 12-13 -0.17025637519469683 1108 14-15 0.08454723064913594 1108 16-17 -0.17058093849561828 1108 18-19 0.13477924121772844 1108 20-21 0.033949378890348214 1108 22-23 -0.014630400197205518 1108 24-25 0.12184656473830557 1108 26-27 0.12281433998821711 1108 28-29 0.019694209014645025 1108 30-31 -0.1407801701031488 1108 32-33 -0.028743970034128097 1108 34-35 -0.19197875947543963 1108 36-37 0.06131307234755923 1108 38-39 -0.18000081601892504 1108 40-41 0.09075906187127458 1108 42-43 2.5010111908585486E-4 1108 44-45 0.036138278968245174 1108 46-47 0.3099201970858019 1108 48-49 0.0699293756110464 1108 50-51 -0.1885249207586881 1108 52-53 0.03936768266165558 1108 54-55 -0.004877983731041979 1108 56-57 0.17604908682741893 1108 58-59 0.14783981364838183 1108 60-61 0.37776535108198317 1108 62-63 0.17394807361926468 1108 64-65 0.0764969816718093 1108 66-67 0.22410487665612067 1108 68-69 0.30539524564223797 1108 70-71 0.37568927752526804 1108 72-73 0.03482757858866847 1108 74-75 -0.17917525148558866 1108 76 0.1796654822043351 1109 1 0.09586258911865286 1109 2 -0.18540933571396323 1109 3 -0.007169737394050912 1109 4 -0.08944297395858314 1109 5 0.07191201811021841 1109 6 -0.3356715419163123 1109 7 0.18347357670534592 1109 8 -0.15990513394092432 1109 9 0.013655189266913226 1109 10-11 0.02546146430598384 1109 12-13 0.11079429665247886 1109 14-15 0.08850546280066851 1109 16-17 0.1481660260502764 1109 18-19 0.08358124866471428 1109 20-21 0.13681889483660115 1109 22-23 0.07058086740842384 1109 24-25 0.030390356222845583 1109 26-27 -0.05240421270726614 1109 28-29 -0.17835500581455932 1109 30-31 0.1983164787555225 1109 32-33 0.2655372344340634 1109 34-35 -0.008588764332159826 1109 36-37 -0.1663621745360473 1109 38-39 -0.020918737339961524 1109 40-41 -0.09077323536038762 1109 42-43 -0.11546950386715338 1109 44-45 0.1224058846020526 1109 46-47 0.16917630846673148 1109 48-49 0.07924214880191727 1109 50-51 0.13325749789114383 1109 52-53 0.17554220103627216 1109 54-55 -0.08249656880470013 1109 56-57 0.13051727526841006 1109 58-59 -0.12779156080522824 1109 60-61 0.0762192931250496 1109 62-63 -0.2428966730215052 1109 64-65 0.056964846331027275 1109 66-67 0.2630072564521342 1109 68-69 0.24916649382096523 1109 70-71 0.5063392694307183 1109 72-73 -0.05809374244095267 1109 74-75 0.360941309952004 1109 76 0.6674265312572771 1110 1 -0.03329901000252988 1110 2 -0.05763657099192443 1110 3 0.02354154982788259 1110 4 0.014413591618726684 1110 5 0.07961730274280399 1110 6 -0.12015896786476077 1110 7 -0.18323363032513384 1110 8 -0.03963364427871596 1110 9 -0.12194055791815117 1110 10-11 -0.21836136122660577 1110 12-13 -0.002925347115422028 1110 14-15 -0.21232626853441872 1110 16-17 0.020707122209408624 1110 18-19 0.20802708804640702 1110 20-21 0.058702844775787355 1110 22-23 -0.12117636119998565 1110 24-25 -0.0318090239019142 1110 26-27 -0.09295503855371123 1110 28-29 0.04600452182481973 1110 30-31 0.05507037259207692 1110 32-33 -0.07065877168622592 1110 34-35 -0.006886069051844856 1110 36-37 0.05073932022639838 1110 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0.354342887903762 2115 58-59 0.23442043952042724 2115 60-61 -0.05303658898844077 2115 62-63 0.05976961371626288 2115 64-65 0.38920045652398017 2115 66-67 0.6185637166003133 2115 68-69 0.3431419069936439 2115 70-71 0.015754721681801698 2115 72-73 0.16301673545532225 2115 74-75 -0.06521272407876921 2115 76 0.12833998888163478 2116 1 -0.04527361357234838 2116 2 -0.09489867397818585 2116 3 -0.06647416732559464 2116 4 0.033359764258428015 2116 5 0.06793058762788462 2116 6 0.10714063842831933 2116 7 0.15278666192055113 2116 8 0.14004765542095043 2116 9 0.17494246599993346 2116 10-11 0.11861985599690428 2116 12-13 0.02596464837173329 2116 14-15 0.04765645815828634 2116 16-17 -0.2982538338914935 2116 18-19 -1.9796291763185536E-4 2116 20-21 0.020456474504548794 2116 22-23 0.07754837075251686 2116 24-25 0.017828392260298642 2116 26-27 -0.11083524583339255 2116 28-29 -0.15172427168913316 2116 30-31 -0.044893057793387925 2116 32-33 -0.16713252715221927 2116 34-35 0.057635958305340296 2116 36-37 -0.1542683827349549 2116 38-39 -0.050016227307700944 2116 40-41 -0.322624679219075 2116 42-43 -0.24308772717346727 2116 44-45 -0.146124290864158 2116 46-47 -0.07840414632908477 2116 48-49 -0.21356609732288234 2116 50-51 -0.16844208508155134 2116 52-53 -0.16597168675978224 2116 54-55 -0.2664685571594134 2116 56-57 -0.07520308038505874 2116 58-59 -0.056865445453901486 2116 60-61 -0.05159957539332538 2116 62-63 -0.1597705419332982 2116 64-65 -0.32008684931773246 2116 66-67 -0.11084985268312408 2116 68-69 -0.3029894586536557 2116 70-71 -0.07769960066134018 2116 72-73 0.03408109428892203 2116 74-75 -0.4240221018034447 2116 76 -0.5192364634708682 2117 1 0.007232256397692538 2117 2 0.01842818214682751 2117 3 -0.029092578790255175 2117 4 -0.034982880061868116 2117 5 -0.011901004955710448 2117 6 0.019625457471313723 2117 7 -0.06400550065738031 2117 8 -0.2727853135694147 2117 9 -0.006063120592244786 2117 10-11 0.08729040898857221 2117 12-13 0.08634032645448286 2117 14-15 0.15733583634629156 2117 16-17 0.13771491357834975 2117 18-19 0.18013156505581662 2117 20-21 -0.08822703290375244 2117 22-23 0.09623916502018659 2117 24-25 -0.10925038678593069 2117 26-27 0.16178235598022184 2117 28-29 -0.4989714581517646 2117 30-31 -0.06526792622509703 2117 32-33 -0.06203982210809045 2117 34-35 0.024662919684992346 2117 36-37 0.16958393691527363 2117 38-39 0.20823006853878923 2117 40-41 -0.43374118800379335 2117 42-43 -0.09675139813128908 2117 44-45 0.009689003605927837 2117 46-47 0.08109710863001851 2117 48-49 -0.1915273960031456 2117 50-51 -0.0507188238071663 2117 52-53 0.05053709795401318 2117 54-55 0.0591649947797066 2117 56-57 -0.3158799486548389 2117 58-59 0.15924661025008646 2117 60-61 -0.09132510369062885 2117 62-63 0.03914047741736226 2117 64-65 0.030245107608834587 2117 66-67 -0.05640486847131143 2117 68-69 -0.024052530469703015 2117 70-71 0.3124833804737932 2117 72-73 -0.28754873325540586 2117 74-75 -0.21860553634315494 2117 76 -0.6714589555767745 2118 1 -0.061731841099010865 2118 2 -0.01609915645659754 2118 3 -0.07753625467958614 2118 4 0.024297422968430737 2118 5 0.04187040479396131 2118 6 0.18555925193771827 2118 7 0.15502447299216016 2118 8 0.24809742687854452 2118 9 0.12802778849866314 2118 10-11 -0.05508030906149486 2118 12-13 0.049342632119831364 2118 14-15 0.006471211840363367 2118 16-17 -0.04553195730439086 2118 18-19 -0.20479103046460523 2118 20-21 -0.03318750721205532 2118 22-23 0.026562781620981468 2118 24-25 0.004814171843847248 2118 26-27 -0.03559083242557648 2118 28-29 0.128367145273792 2118 30-31 -0.0784760948680443 2118 32-33 -0.23617931486171528 2118 34-35 -0.20662413565098348 2118 36-37 -0.07070756506101361 2118 38-39 -0.16746163106595446 2118 40-41 -0.09076523279956916 2118 42-43 -0.2683464244817486 2118 44-45 -0.0036344483044814524 2118 46-47 -0.11453447898525582 2118 48-49 -0.019265373605250602 2118 50-51 -0.02722985806276057 2118 52-53 -0.2739976517166056 2118 54-55 0.12165676026059202 2118 56-57 -0.24406259028857136 2118 58-59 -0.09339255312277572 2118 60-61 -0.12048765309774723 2118 62-63 0.05324209138310465 2118 64-65 -0.22432916775348133 2118 66-67 -0.3965357973250647 2118 68-69 -0.3881294408781315 2118 70-71 -0.7107461979188301 2118 72-73 -0.45439655934236356 2118 74-75 -0.4657152201376249 2118 76 -0.7961378093317109 2119 1 0.04035258384203644 2119 2 -0.0866797826027863 2119 3 -0.07936196859103717 2119 4 -0.030564052011918363 2119 5 -0.1601698100344393 2119 6 -0.08995980753307009 2119 7 -0.08080372978488981 2119 8 0.06097403154188896 2119 9 -0.04871441742221805 2119 10-11 -0.15158525404446976 2119 12-13 -0.18416044621394434 2119 14-15 -0.6708686493071383 2119 16-17 -0.0938642536103913 2119 18-19 -0.05143924896080421 2119 20-21 -0.2606239756427655 2119 22-23 -0.20122982382832788 2119 24-25 -0.09307029239509035 2119 26-27 -0.20480913213739171 2119 28-29 -0.06045915810582869 2119 30-31 -0.23871319206479313 2119 32-33 -0.6051004243717983 2119 34-35 -0.36801970154042607 2119 36-37 -0.20059523862664008 2119 38-39 -0.02107703583409659 2119 40-41 -0.09843650605081677 2119 42-43 -0.094817638786175 2119 44-45 0.05090773141781568 2119 46-47 0.040041267674425285 2119 48-49 -0.009035644760615469 2119 50-51 -0.23555363982854516 2119 52-53 -0.17402958124241508 2119 54-55 -0.14935941736580816 2119 56-57 -0.021083974883801204 2119 58-59 0.0036854859131167927 2119 60-61 0.1017459400382279 2119 62-63 -0.2107864323266142 2119 64-65 -0.24238572394944669 2119 66-67 -0.14839835299706294 2119 68-69 -0.2759299059030269 2119 70-71 -0.07753457884626869 2119 72-73 -0.7506861847025945 2119 74-75 -0.1576607925767135 2119 76 -0.9467878617288861 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 2.0 21 2.0 22 8.0 23 10.0 24 32.0 25 65.0 26 116.0 27 232.0 28 422.0 29 659.0 30 1093.0 31 1645.0 32 2657.0 33 4345.0 34 6995.0 35 12080.0 36 17861.0 37 86086.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.298618142831614 15.36571564714992 10.526981952468878 32.80868425754959 2 21.134373697063555 20.132973971052476 32.45205193876943 26.28060039311454 3 18.74143784620883 19.48820656382155 33.84582166894991 27.924533921019716 4 16.944130085174816 22.34647685984871 32.75284412412889 27.956548930847582 5 16.59717672285425 29.734498778962415 34.97230329382334 18.696021204359997 6 27.52695217106439 35.97370301983441 19.11668354279588 17.38266126630532 7 26.946214783489193 27.89475251652868 20.876764548216094 24.282268151766036 8 24.086455417237477 33.763178271487284 17.96116504854369 24.18920126273155 9 22.627166597176725 17.54050271010781 19.046697242241944 40.785633450473526 10-11 25.491765441658227 25.52601405682292 24.884597057597237 24.097623443921616 12-13 23.235824051462266 18.90262969801656 27.854919888021918 30.006626362499254 14-15 19.57382810173328 19.454702483769136 23.72684495800822 37.24462445648937 16-17 26.81182619572339 25.800747513252727 21.74116981356841 25.646256477455477 18-19 28.260319256656143 24.328057061171005 21.60119721246054 25.81042646971231 20-21 23.879846923580917 22.728423372446244 21.712505211745786 31.679224492227053 22-23 22.943966287450117 26.857987372684494 21.664110429447852 28.533935910417537 24-25 27.056033712549883 25.767615700756448 22.56574245041396 24.61060813627971 26-27 27.58874858538329 27.115596521531955 21.886354160462208 23.40930073262255 28-29 23.975891953064508 27.073530287688367 22.15810947644291 26.792468282804215 30-31 23.0232592769075 24.242435523259275 29.14780511048901 23.586500089344213 32-33 26.138394186669846 22.22102269343022 25.410983381976294 26.229599737923643 34-35 26.007728274465425 21.788447793197925 23.112603490380607 29.091220441956047 36-37 28.86078682470665 24.21674906188576 23.2518315563762 23.67063255703139 38-39 22.436565608434094 24.1367115373161 27.81657632973971 25.610146524510096 40-41 21.836470307939724 21.123205670379416 28.950503305735896 28.089820715944963 42-43 25.29744177735422 20.94265590565251 28.253246173089526 25.506656143903744 44-45 20.829858836142712 25.123592828637797 30.485362439692654 23.561185895526833 46-47 23.95727857525761 22.34498778962416 28.105828220858896 25.591905414259337 48-49 23.012463517779498 21.596730001786884 25.624292691643337 29.766513788790277 50-51 21.36852998987432 21.943311096551312 29.034635773423073 27.653523140151286 52-53 18.670707010542618 21.937727083209243 33.92958186908094 25.46198403716719 54-55 18.802117457859314 22.435448805765677 30.6741020906546 28.08833164572041 56-57 22.54080052415272 23.18966287450116 26.24262910238847 28.026907498957648 58-59 19.61105485734707 23.506090297218414 30.608583000774313 26.274271844660195 60-61 22.783146703198522 26.48869795699565 25.818244088391207 24.909911251414616 62-63 19.13939186372029 32.35675144439812 23.145735302876883 25.358121389004708 64-65 18.60146524510096 33.62916195127762 22.40120019060099 25.36817261302043 66-67 18.946185002084697 33.51524807909941 22.12199952349753 25.416567395318364 68-69 19.0943474894276 33.68611888736673 21.769089880278756 25.450443742926915 70-71 19.038879623563044 32.872342009649174 22.156248138662217 25.93253022812556 72-73 19.255539341235334 30.158511525403537 23.37505211745786 27.210897015903267 74-75 19.860101852403357 28.822815533980584 23.52954315325511 27.78753946036095 76 19.90737983203288 29.0956876526297 23.237685389242955 27.759247126094465 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 3.0 14 8.0 15 10.5 16 21.5 17 33.0 18 35.0 19 55.0 20 98.5 21 124.0 22 153.5 23 253.5 24 349.5 25 375.0 26 457.0 27 627.0 28 807.5 29 900.0 30 1018.5 31 1259.5 32 1629.0 33 1876.0 34 2063.5 35 2543.0 36 2948.5 37 3062.0 38 3334.0 39 4007.0 40 4799.0 41 6964.5 42 8739.0 43 9532.5 44 9855.0 45 8582.0 46 7780.0 47 7376.5 48 6464.5 49 5696.0 50 5436.0 51 5007.5 52 4034.0 53 3457.0 54 3425.0 55 3232.5 56 2832.0 57 2571.0 58 2518.0 59 2446.5 60 2539.0 61 2729.0 62 2755.0 63 2796.0 64 2893.0 65 2854.0 66 2699.5 67 2640.0 68 2580.5 69 2430.5 70 2214.0 71 2088.0 72 1933.0 73 1571.0 74 1124.0 75 884.0 76 738.0 77 419.5 78 167.0 79 87.0 80 59.0 81 19.0 82 6.0 83 5.0 84 3.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 134312.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.21573649413306 #Duplication Level Percentage of deduplicated Percentage of total 1 64.67090629099413 33.76839001727321 2 21.114469856841385 22.050151885162904 3 7.390349626418753 11.576776460777891 4 2.756231107055267 5.756745488117219 5 1.3360520161980265 3.4881470010125675 6 0.8070495636799178 2.528441241288939 7 0.527576569896766 1.928345940794568 8 0.3578965379569954 1.495026505449997 9 0.22671533679347516 1.0654297456668056 >10 0.7870872069811213 5.867681219846328 >50 0.011407060970740887 0.4444874620287093 >100 0.008555295728055665 1.0081005420215619 >500 0.0 0.0 >1k 0.004277647864027833 3.8298886175472036 >5k 0.0014258826213426109 5.192387873012091 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 6974 5.192387873012091 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 2387 1.7772053130025611 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 1420 1.057239859431771 RNA PCR Primer, Index 18 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 1337 0.9954434451128715 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 435 0.3238727738400143 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT 263 0.19581273452856035 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 248 0.18464470784442194 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 191 0.14220620644469592 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005211745785931265 0.0 2 0.0 0.0 0.0 0.005956280898207159 0.0 3 0.0 0.0 0.0 0.006700816010483055 0.0 4 0.0 0.0 0.0 0.011168026684138424 0.0 5 0.0 0.0 0.0 0.01637977247006969 0.0 6 0.0 0.0 0.0 0.01637977247006969 0.0 7 0.0 0.0 0.0 0.01637977247006969 0.0 8 0.0 0.0 0.0 0.017124307582345583 0.0 9 0.0 0.0 0.0 0.020102448031449165 0.0 10 0.0 0.0 0.0 0.02084698314372506 0.0 11 0.0 0.0 0.0 0.021591518256000954 0.0 12 0.0 0.0 0.0 0.021591518256000954 0.0 13 0.0 0.0 0.0 0.023825123592828637 0.0 14 0.0 0.0 0.0 0.02456965870510453 0.0 15 0.0 0.0 0.0 0.02456965870510453 0.0 16 0.0 0.0 0.0 0.02456965870510453 0.0 17 0.0 0.0 0.0 0.027547799154208113 0.0 18 0.0 0.0 0.0 0.029781404491035796 0.0 19 0.0 0.0 0.0 0.03201500982786348 0.0 20 0.0 0.0 0.0 0.03499315027696706 0.0 21 0.0 0.0 0.0 0.04020489606289833 0.0 22 0.0 0.0 0.0 0.044672106736553696 0.0 23 0.0 0.0 0.0 0.04690571207338138 0.0 24 0.0 0.0 0.0 0.056584668532968015 0.0 25 0.0 0.0 0.0 0.06849723032938233 0.0 26 0.0 0.0 0.0 0.11093573172910834 0.0 27 0.0 0.0 0.0 0.18687831318124962 0.0 28 0.0 0.0 0.0 0.31047114181904817 0.0 29 0.0 0.0 0.0 0.4169396628745012 0.0 30 0.0 0.0 0.0 0.5636130799928525 0.0 31 0.0 0.0 0.0 0.7318780153672048 0.0 32 0.0 0.0 0.0 0.9358806361308 0.0 33 0.0 0.0 0.0 1.1920007147537077 0.0 34 0.0 0.0 0.0 1.629042825659658 0.0 35 0.0 0.0 0.0 2.1427720531300256 0.0 36 0.0 0.0 0.0 2.804663767943296 0.0 37 0.0 0.0 0.0 3.6526892608255404 0.0 38 0.0 0.0 0.0 4.517094526177854 0.0 39 0.0 0.0 0.0 5.389689677765204 0.0 40 0.0 0.0 0.0 6.257073083566621 0.0 41 0.0 0.0 0.0 7.119244743582107 0.0 42 0.0 0.0 0.0 7.948656858657454 0.0 43 0.0 0.0 0.0 8.862945976532254 0.0 44 0.0 0.0 0.0 9.761599857049259 0.0 45 0.0 0.0 0.0 10.678122580260885 0.0 46 0.0 0.0 0.0 11.648996366668651 0.0 47 0.0 0.0 0.0 12.61242480195366 0.0 48 0.0 0.0 0.0 13.540115551849425 0.0 49 0.0 0.0 0.0 14.370272202037048 0.0 50 0.0 0.0 0.0 15.212341414021084 0.0 51 0.0 0.0 0.0 16.01122758949312 0.0 52 0.0 0.0 0.0 16.741616534635774 0.0 53 0.0 0.0 0.0 17.43328965394008 0.0 54 0.0 0.0 0.0 18.170379415093215 0.0 55 0.0 0.0 0.0 18.96405384477932 0.0 56 0.0 0.0 0.0 19.70263267615701 0.0 57 0.0 0.0 0.0 20.48960628983263 0.0 58 0.0 0.0 0.0 21.20212639228066 0.0 59 0.0 0.0 0.0 21.897522187146347 0.0 60 0.0 0.0 0.0 22.57281553398058 0.0 61 0.0 0.0 0.0 23.274912144856753 0.0 62 0.0 0.0 0.0 23.911489665852642 0.0 63 0.0 0.0 0.0 24.62103162785157 0.0 64 0.0 0.0 0.0 25.316427422717254 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGACGT 15 0.0022206167 70.0 23 CCGGGGG 20 0.006938743 52.5 14 TGAGTCG 20 0.006938743 52.5 66 GGGCATA 20 0.006938743 52.5 39 TGAATTA 20 0.006938743 52.5 19 GATTATC 20 0.006938743 52.5 50 TCGAAAT 20 0.006938743 52.5 70 AACTCGT 55 2.2610038E-9 50.90909 37 TCTTTAT 85 1.8189894E-12 45.29412 1 CCTTTAA 55 1.3010504E-7 44.545452 1 ACACAAG 40 4.5730583E-5 43.75 27 CGGAACT 65 9.911673E-9 43.076927 34 CCGGAAC 65 9.911673E-9 43.076927 33 ATCGTAT 50 3.4578989E-6 42.0 39 CGTCAGA 35 0.0012518136 40.0 2 ACTCGTA 80 1.4060788E-9 39.375 38 CCTTATC 45 9.158561E-5 38.888885 17 CTCTCCA 45 9.158561E-5 38.888885 9 CTTTATA 100 7.2759576E-12 38.5 2 GCCGGAA 85 2.5429472E-9 37.058826 32 >>END_MODULE