FastQCFastQC Report
Wed 25 May 2016
SRR1780171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489149
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA301.3094806E-436.62141832
CGTAACA300.005687508729.39030317
CGTTTAT300.00577646329.29713433
GTCTGAC300.005785129529.2881492
CGTCAGA508.419283E-526.3593332
GACGAAA1051.10958354E-1025.14783123
TGCGCAA450.001396245924.44677510
TCAGATG752.0878852E-623.4305174
GAACTAT500.00260144221.9661126
GAATTAG550.00452000419.969191
CGACGAA1851.8189894E-1219.0307922
GAATAGA600.00747209118.3050921
CTCTAGC600.00747209118.3050922
GCGTGTA750.001277972417.62333534
TTAGGCC750.001302773517.572895
GGTCTTT750.001302773517.572891
CTGCCGA3800.017.40215318
AACTATC1053.817052E-516.7360847
TGCCGAC3750.016.4585719
TAGATCT1501.8036008E-716.15472239
Produced by FastQC (version 0.11.5)