FastQCFastQC Report
Wed 25 May 2016
SRR1780171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489149
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT150543.077589855033947No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG50251.027294341805871No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC29600.6051325874120156No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG27060.5532056694381466TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC8360.1709090686069071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG207.853836E-443.9979611
ATGCCGT21450.033.9471444
GTCTTCT3750.030.50524943
TCTTCTG3700.030.32291644
CGTCTTC3700.030.32291642
CCGTCTT3700.030.32291641
TATGCCG26150.027.92986943
TTTATAC1650.025.3373363
GCCGTCT4600.024.39017140
CTCGTAT30500.023.80217239
AGACGTG30600.023.7962825
CGTATGC30700.023.79042441
GTATGCC30800.023.71318442
TCGTATG30800.023.71318440
GAGACGT31000.023.6311624
TCTCGTA30600.023.58060338
GACGTGT30950.023.52717826
ACGTGTG31100.023.48443827
ATCTCGT30900.023.35166537
ACGAGAC31600.023.32170122
Produced by FastQC (version 0.11.5)