FastQCFastQC Report
Wed 25 May 2016
SRR1780170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68096
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTC150.002215895670.0000137
ATTTGCC150.002215895670.0000126
AGAGGCC200.00692410652.58
GACTCTT200.00692410652.514
GTTGCGG200.00692410652.522
ATCTCTG400.002403528435.042
TTATATC400.002403528435.038
GTAGATC806.104892E-835.038
GATCTCT450.00428031131.11110941
TAGATCT1051.9679646E-830.00000239
TTATACC952.7591523E-729.4736862
CATCTTA604.9357646E-429.1666689
TGTAGAT1004.3224463E-728.00000237
ATATCTC657.8822306E-426.92307940
TTTATAT657.8822306E-426.92307937
AGATCTC1056.6206485E-726.66666840
TATACCC1056.6206485E-726.6666683
TATATCT808.553635E-526.2539
ACTCTTC851.2914263E-424.70588315
ATTATAC851.2914263E-424.7058831
Produced by FastQC (version 0.11.5)