##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68096 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.339638157894736 34.0 23.0 34.0 12.0 34.0 2 28.446707589285715 34.0 23.0 34.0 12.0 34.0 3 28.193888040413533 34.0 23.0 34.0 11.0 34.0 4 28.227986959586467 34.0 23.0 34.0 11.0 34.0 5 28.13422227443609 34.0 23.0 34.0 11.0 34.0 6 30.667763157894736 37.0 26.0 38.0 11.0 38.0 7 30.959850798872182 37.0 26.0 38.0 12.0 38.0 8 30.79423167293233 37.0 26.0 38.0 11.0 38.0 9 31.167586936090224 37.0 26.0 38.0 12.0 38.0 10-11 30.811648261278194 37.0 26.0 38.0 11.0 38.0 12-13 30.915391506109025 37.0 26.0 38.0 11.0 38.0 14-15 30.709468984962406 37.0 26.0 38.0 11.0 38.0 16-17 30.75673314144737 37.0 26.0 38.0 11.0 38.0 18-19 30.7890625 37.0 26.0 38.0 11.0 38.0 20-21 30.807139920112782 37.0 26.0 38.0 11.0 38.0 22-23 30.784965343045116 37.0 25.5 38.0 11.0 38.0 24-25 30.766843867481203 37.0 26.0 38.0 11.0 38.0 26-27 30.66241042058271 37.0 25.0 38.0 11.0 38.0 28-29 30.735872885338345 37.0 25.5 38.0 11.0 38.0 30-31 30.736306097274436 37.0 26.0 38.0 11.0 38.0 32-33 30.81484962406015 37.0 26.0 38.0 11.0 38.0 34-35 30.770052572838345 37.0 25.5 38.0 11.0 38.0 36-37 30.69760338345865 37.0 25.5 38.0 11.0 38.0 38-39 30.631879992951127 37.0 25.0 38.0 11.0 38.0 40-41 30.722634222274436 37.0 26.0 38.0 11.0 38.0 42-43 30.747268562030072 37.0 26.0 38.0 11.0 38.0 44-45 30.87398672462406 37.0 26.0 38.0 11.0 38.0 46-47 30.79697779605263 37.0 25.5 38.0 11.0 38.0 48-49 30.689967105263158 37.0 25.5 38.0 11.0 38.0 50-51 30.60496211231203 37.0 25.0 38.0 11.0 38.0 52-53 30.728023672462406 37.0 25.0 38.0 11.0 38.0 54-55 30.67493685385338 37.0 25.0 38.0 11.0 38.0 56-57 30.649149729793233 37.0 25.0 38.0 11.0 38.0 58-59 30.65365807095865 37.0 25.0 38.0 11.0 38.0 60-61 30.710122474154133 37.0 25.0 38.0 11.0 38.0 62-63 30.56778665413534 37.0 25.0 38.0 11.0 38.0 64-65 30.476643268327066 37.0 25.0 38.0 11.0 38.0 66-67 30.449483082706767 37.0 24.5 38.0 11.0 38.0 68-69 30.36368509163534 37.0 24.0 38.0 11.0 38.0 70-71 30.250418526785715 37.0 24.0 38.0 11.0 38.0 72-73 30.25899465460526 37.0 24.0 38.0 11.0 38.0 74-75 30.269758869830827 37.0 24.0 38.0 11.0 38.0 76 28.56675869360902 34.0 20.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3303206258930125 1101 2 -0.47236965948341947 1101 3 0.02939561064195928 1101 4 -0.22782008572928447 1101 5 -0.5717074849048451 1101 6 -1.047847342110913 1101 7 -1.1393018905242194 1101 8 -1.0058312705308374 1101 9 -0.80821140209828 1101 10-11 -1.2561866983134173 1101 12-13 -0.3626143339027408 1101 14-15 -0.23015922542094813 1101 16-17 -0.7925908871086378 1101 18-19 -0.6301585883116054 1101 20-21 -0.13687883027019865 1101 22-23 -1.0506075531591605 1101 24-25 -0.7570147947432595 1101 26-27 -0.8155620219297752 1101 28-29 -0.9649107592985189 1101 30-31 -0.3938862364500082 1101 32-33 -0.5385505865443463 1101 34-35 -0.4350244232153564 1101 36-37 -1.5353555056510544 1101 38-39 -0.2983299775481889 1101 40-41 -0.39574678870758717 1101 42-43 -1.1080477580601382 1101 44-45 -0.6995131453058292 1101 46-47 -1.1694604823873895 1101 48-49 -1.0453161927448988 1101 50-51 -0.15468947513237552 1101 52-53 -0.9232027664899078 1101 54-55 -0.6492461972680701 1101 56-57 -0.4057404104702549 1101 58-59 -0.6705201579816205 1101 60-61 -0.33667778884178645 1101 62-63 -0.8582427131434827 1101 64-65 -0.2533374901522727 1101 66-67 -0.81722461867178 1101 68-69 -0.0750001688749542 1101 70-71 -0.2253740363667518 1101 72-73 -0.8533634211175425 1101 74-75 -0.9229039593291155 1101 76 -2.0212209505578187 1102 1 -0.4150303659950261 1102 2 0.40533255086392117 1102 3 -0.5964241610364454 1102 4 0.01583427424156625 1102 5 -0.22564238896119804 1102 6 0.6957159321178956 1102 7 -0.5926356286830732 1102 8 -0.06922694699428078 1102 9 -0.17920362944829193 1102 10-11 0.09057672165257813 1102 12-13 0.424973715647905 1102 14-15 -0.1764034566572832 1102 16-17 -0.5151892597100485 1102 18-19 -0.015147658022559796 1102 20-21 0.17243316875822146 1102 22-23 -0.32714386600832057 1102 24-25 -0.38157454699004134 1102 26-27 -0.2999256362120164 1102 28-29 -0.1658914491212009 1102 30-31 0.22177747499036116 1102 32-33 0.3811725127998393 1102 34-35 -0.31114781403391234 1102 36-37 0.33410344504119394 1102 38-39 -0.6569193630230501 1102 40-41 -0.04488451034955787 1102 42-43 0.09187026477201954 1102 44-45 0.2617905952819797 1102 46-47 -0.4322428481877303 1102 48-49 -0.91295342859625 1102 50-51 -0.1496342164488631 1102 52-53 -0.2460258172550276 1102 54-55 0.05763988483054305 1102 56-57 0.17808883412532595 1102 58-59 0.016903937233340827 1102 60-61 -0.1690192996506248 1102 62-63 -0.1318946441612141 1102 64-65 0.020466250435536182 1102 66-67 -0.22975194439439406 1102 68-69 0.3482752744089872 1102 70-71 0.01140335007968929 1102 72-73 0.15343764762183554 1102 74-75 -0.03732283229486555 1102 76 0.09977127679219322 1103 1 -0.34437819208198306 1103 2 0.45968037695087816 1103 3 -0.6181632914712267 1103 4 -0.08199181271495348 1103 5 0.2960967414735869 1103 6 -0.059718850490799724 1103 7 0.01606002349084079 1103 8 0.09381653126659018 1103 9 -0.4400731946656826 1103 10-11 0.02807672165257813 1103 12-13 0.13421284608268635 1103 14-15 -0.0595556305703262 1103 16-17 -0.20268925971004492 1103 18-19 -0.5015607015008179 1103 20-21 -0.012349439937430873 1103 22-23 -0.26736125731266824 1103 24-25 0.19179501822734935 1103 26-27 -0.4520995492554931 1103 28-29 -0.04089144912120446 1103 30-31 -0.3026790467487679 1103 32-33 -0.31991444372189903 1103 34-35 0.00135218596608766 1103 36-37 -0.6658965549588061 1103 38-39 -0.9177889282404408 1103 40-41 -0.41444972774086253 1103 42-43 -0.5005210395758084 1103 44-45 -0.545274622109325 1103 46-47 -0.5816993699268558 1103 48-49 -0.16023603729189873 1103 50-51 -0.49474291210104226 1103 52-53 -0.5204823389941602 1103 54-55 -0.6352948977781523 1103 56-57 -0.734954644135545 1103 58-59 -0.5048351932014441 1103 60-61 -0.0820627779114993 1103 62-63 -0.7324381224220815 1103 64-65 -0.8708380973905498 1103 66-67 -0.6264910748291754 1103 68-69 -0.6000942908084035 1103 70-71 -1.0212053455724828 1103 72-73 0.15615503892617966 1103 74-75 0.3784380372703531 1103 76 -0.38392437538172075 1104 1 0.36868746896504234 1104 2 1.0393165792578856 1104 3 0.5533096455296587 1104 4 1.4325379122184962 1104 5 0.39103906622957396 1104 6 0.8714035984887936 1104 7 0.3810777697197665 1104 8 0.005085386634821276 1104 9 0.5198868763192337 1104 10-11 0.3552173605168214 1104 12-13 1.1180748691527853 1104 14-15 0.8761142895716425 1104 16-17 0.4708910419758432 1104 18-19 0.6410191565293495 1104 20-21 0.49580273397561214 1104 22-23 -0.10010304968179184 1104 24-25 0.2688802001261976 1104 26-27 0.04302023778975794 1104 28-29 0.4430814878796845 1104 30-31 1.6783546710861899 1104 32-33 1.0771574285052523 1104 34-35 1.2052674477229672 1104 36-37 0.19185632880339654 1104 38-39 0.3724173716708279 1104 40-41 0.11904627935762946 1104 42-43 0.5264865025714869 1104 44-45 0.9279951205703547 1104 46-47 0.2928015173845857 1104 48-49 0.013898834047939346 1104 50-51 0.2777726158492726 1104 52-53 0.6586436592312204 1104 54-55 0.31263101171608554 1104 56-57 1.1087676273817557 1104 58-59 0.32923756633715584 1104 60-61 0.8275423684948926 1104 62-63 0.3514682662203299 1104 64-65 0.8537626124586062 1104 66-67 0.8058514273891042 1104 68-69 0.5765361439742058 1104 70-71 0.4074215399643393 1104 72-73 0.791303663593439 1104 74-75 0.29658355634754585 1104 76 1.0333338322491592 1105 1 0.6587439170762011 1105 2 0.6079227451284908 1105 3 0.3864620554297886 1105 4 0.3503624888576624 1105 5 0.790083790502262 1105 6 0.591876894190051 1105 7 0.3609952686342268 1105 8 0.5718461334867548 1105 9 0.4945937803574445 1105 10-11 0.5424731138819965 1105 12-13 -0.16660815299224296 1105 14-15 0.11667008635844311 1105 16-17 0.2530230437127088 1105 18-19 0.7022436463252681 1105 20-21 1.0091006063160357 1105 22-23 1.744664644629978 1105 24-25 1.1698246204475176 1105 26-27 0.3981548448240595 1105 28-29 0.8108083658649221 1105 30-31 0.6774319615768576 1105 32-33 -0.16242138174225218 1105 34-35 0.5199113904064205 1105 36-37 0.3325423904621019 1105 38-39 0.5119636157003526 1105 40-41 0.0989637782720898 1105 42-43 0.922871652376088 1105 44-45 0.1174219551339668 1105 46-47 0.4686590944579727 1105 48-49 0.9064845917552802 1105 50-51 0.5680461720802761 1105 52-53 0.7501004547153691 1105 54-55 0.665757368641831 1105 56-57 0.8632183438385539 1105 58-59 0.7655163331630348 1105 60-61 0.5815588687119977 1105 62-63 1.0617917573189004 1105 64-65 0.5290231422024156 1105 66-67 0.8278336245232794 1105 68-69 0.9621744418465461 1105 70-71 0.666757189117618 1105 72-73 0.7873414404062942 1105 74-75 0.679779387871644 1105 76 0.8408674840077346 1106 1 0.997447643555109 1106 2 1.4559734154027488 1106 3 1.4836210764604125 1106 4 0.675076546169187 1106 5 1.5496152134514674 1106 6 1.7141250801450418 1106 7 0.5791726145941318 1106 8 0.5640980090248178 1106 9 1.380893128531099 1106 10-11 1.389678254701085 1106 12-13 1.513689473375976 1106 14-15 1.8914056662379188 1106 16-17 0.9932456485583607 1106 18-19 1.112648270602726 1106 20-21 0.9192131385998898 1106 22-23 1.0861509316795406 1106 24-25 1.1707470162167972 1106 26-27 1.2743078395356626 1106 28-29 0.9499825141861606 1106 30-31 1.2047348763474872 1106 32-33 1.5396294115181135 1106 34-35 1.3346174536212771 1106 36-37 1.636456423176405 1106 38-39 0.9113609837977599 1106 40-41 1.085927251399628 1106 42-43 1.2905539792731489 1106 44-45 1.1903373406954003 1106 46-47 1.3017669532699294 1106 48-49 1.2834369961335845 1106 50-51 0.892037686039199 1106 52-53 0.9339031850068444 1106 54-55 0.42890150835404484 1106 56-57 0.5265746345777025 1106 58-59 0.9856460835012477 1106 60-61 1.39211415096511 1106 62-63 1.6332159363866623 1106 64-65 1.0093453657911517 1106 66-67 0.7549489854874878 1106 68-69 1.1610737162285396 1106 70-71 1.2024846519143182 1106 72-73 1.3298638853700098 1106 74-75 0.8485624403867114 1106 76 0.9110618020497938 1107 1 0.20661625750173584 1107 2 0.10260359619232062 1107 3 -0.26779326371914536 1107 4 0.14291568034702706 1107 5 0.013488045821411276 1107 6 0.8312385963177107 1107 7 0.7652505877831608 1107 8 0.9441865590186715 1107 9 0.6807639931234029 1107 10-11 0.6621539649458263 1107 12-13 0.9397748257311633 1107 14-15 0.7975211501882278 1107 16-17 0.4498315543510074 1107 18-19 0.024052156963566773 1107 20-21 0.5569729467415705 1107 22-23 0.45477102760870025 1107 24-25 0.5129097268304967 1107 26-27 0.5470910150368269 1107 28-29 0.7762338977798144 1107 30-31 0.009878770087496491 1107 32-33 0.8881105331513659 1107 34-35 0.19012415636386848 1107 36-37 0.500095581951463 1107 38-39 0.4215380837854603 1107 40-41 0.48726165061251336 1107 42-43 0.25265888641864365 1107 44-45 0.8222091891765189 1107 46-47 0.4872761157345664 1107 48-49 0.4649952300531517 1107 50-51 0.12123766144197745 1107 52-53 0.6782919440770705 1107 54-55 0.5380977941737441 1107 56-57 0.9137502587321684 1107 58-59 0.2681759076311181 1107 60-61 0.2969844006268936 1107 62-63 0.4314726083827267 1107 64-65 0.47849122730879756 1107 66-67 0.391663411757321 1107 68-69 0.7839829524848447 1107 70-71 0.6481401678410208 1107 72-73 0.5586180361509747 1107 74-75 0.3553113027652621 1107 76 0.49512280315666857 1108 1 1.2558493104458215 1108 2 0.3340484254847489 1108 3 0.7678326640393891 1108 4 1.366567337942275 1108 5 1.2169764179144344 1108 6 1.6931881262533857 1108 7 1.50127235918346 1108 8 1.2530683007306926 1108 9 1.2974141663049963 1108 10-11 1.5128466913189094 1108 12-13 1.2339979825842065 1108 14-15 1.8887507395004484 1108 16-17 1.9716029546478886 1108 18-19 1.4202669021392218 1108 20-21 2.2341748269988706 1108 22-23 1.640879389805633 1108 24-25 1.9615119039705284 1108 26-27 1.6145081352743382 1108 28-29 1.7826045063894185 1108 30-31 1.9805616003695334 1108 32-33 1.4863292367634386 1108 34-35 1.6556115388477863 1108 36-37 2.2171292286610083 1108 38-39 1.5945341253490426 1108 40-41 1.7043571478910913 1108 42-43 1.9309122263493705 1108 44-45 1.6072784618138272 1108 46-47 2.096194967788005 1108 48-49 1.9727265511813066 1108 50-51 2.0522742769788387 1108 52-53 2.156211290935058 1108 54-55 2.2583476704726095 1108 56-57 1.7803385813447399 1108 58-59 2.097035383138291 1108 60-61 1.8755964738579642 1108 62-63 2.314389481217958 1108 64-65 2.06217504719994 1108 66-67 2.223862817991858 1108 68-69 1.6201407951369973 1108 70-71 2.0281500639320598 1108 72-73 1.5864507922123252 1108 74-75 2.404853608554479 1108 76 1.2161767368528587 1109 1 -0.09791228569091714 1109 2 0.6401864353970623 1109 3 0.9107034227126647 1109 4 1.143031945897544 1109 5 0.7798814884443637 1109 6 1.5229314227332473 1109 7 1.284027474191717 1109 8 0.8918089285105886 1109 9 0.8977445767764678 1109 10-11 1.1036142602603256 1109 12-13 0.9457625015828057 1109 14-15 1.2770148137157236 1109 16-17 1.6556797115420316 1109 18-19 1.5547026627187073 1109 20-21 1.6993546465439202 1109 22-23 0.8176648771610786 1109 24-25 0.28213714224731135 1109 26-27 0.8609498686584978 1109 28-29 1.4056665218932949 1109 30-31 1.1887678527522993 1109 32-33 0.88212285729972 1109 34-35 1.645492718159005 1109 36-37 1.471116393485005 1109 38-39 1.8072199812392427 1109 40-41 1.1968593718077223 1109 42-43 1.088826192545838 1109 44-45 1.2852076777243653 1109 46-47 1.138290532662829 1109 48-49 1.8431198667233097 1109 50-51 1.5280653558961141 1109 52-53 1.879969074643281 1109 54-55 1.5838337551085218 1109 56-57 1.843656664964005 1109 58-59 1.435976163415333 1109 60-61 0.9571983292398478 1109 62-63 1.4276503784094672 1109 64-65 1.3570663929872104 1109 66-67 1.495212417686865 1109 68-69 1.1988039035370477 1109 70-71 1.6115310526218636 1109 72-73 1.4619432308957698 1109 74-75 1.7921645756405091 1109 76 0.9449542181084176 1110 1 -0.6552893348712452 1110 2 -0.7587206684280723 1110 3 -0.9472200745550978 1110 4 -1.0099735185833296 1110 5 -1.3294081290419761 1110 6 -0.41149480677495376 1110 7 0.0982351244649422 1110 8 -0.4743112900686448 1110 9 -0.3208346581962118 1110 10-11 -1.0166043189746503 1110 12-13 -1.0515052579800361 1110 14-15 -0.7585043119673323 1110 16-17 -0.5601672829934863 1110 18-19 -0.7103246596856607 1110 20-21 -0.6257819654779411 1110 22-23 -0.6850673632760795 1110 24-25 -1.1249666828893048 1110 26-27 -0.8658260876256527 1110 28-29 -0.8866832048826652 1110 30-31 -0.9560408904170998 1110 32-33 -0.9894618421538333 1110 34-35 -1.0648897955021965 1110 36-37 -0.8950038250942285 1110 38-39 -0.8758401280503705 1110 40-41 -1.251228059886266 1110 42-43 -1.0313923866891344 1110 44-45 -0.7830983332045989 1110 46-47 -1.5693597405612145 1110 48-49 -1.3481369638777885 1110 50-51 -0.6850493982022492 1110 52-53 -0.6227631657938737 1110 54-55 -1.4325596875144306 1110 56-57 -1.5388569962381844 1110 58-59 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38-39 0.06837133465136702 2116 40-41 0.047380403705041374 2116 42-43 -0.46443661085993426 2116 44-45 -0.18341921260477534 2116 46-47 0.011892642206607462 2116 48-49 -0.95750600695823 2116 50-51 0.30808823046720946 2116 52-53 0.17365315140017756 2116 54-55 -0.07595465510878796 2116 56-57 -0.7545451395854919 2116 58-59 0.23947724360341027 2116 60-61 0.020945311067269046 2116 62-63 -0.15654075134018086 2116 64-65 -0.24887146442796393 2116 66-67 -0.09590462386860565 2116 68-69 -0.5571847862583503 2116 70-71 -0.24510575002142332 2116 72-73 -0.4368050217411579 2116 74-75 -0.3364254612129649 2116 76 0.8905953415040209 2117 1 0.1836458545539159 2117 2 -0.3829841846230835 2117 3 -0.5292636072351335 2117 4 -0.3339653371259317 2117 5 0.2383483810169409 2117 6 -0.5059479007701277 2117 7 -0.3348326167812026 2117 8 0.37964358980435975 2117 9 -0.10430078757313765 2117 10-11 0.23543341341842705 2117 12-13 -0.47993276965693 2117 14-15 -0.16312011927569614 2117 16-17 -1.4154959150415998 2117 18-19 -0.7195440631719379 2117 20-21 -0.6229123498232703 2117 22-23 -0.5953002905893285 2117 24-25 -0.9329924483745451 2117 26-27 -0.5501988934381536 2117 28-29 -0.8699386922594101 2117 30-31 -0.9693052308634122 2117 32-33 -0.3569863407342879 2117 34-35 -0.03278978634384799 2117 36-37 -0.5828415391706088 2117 38-39 -0.17542494961522692 2117 40-41 -0.45440600658468 2117 42-43 -0.16391367984785177 2117 44-45 -0.713479625752754 2117 46-47 -0.30074539858850713 2117 48-49 -0.5900089301750668 2117 50-51 0.19167923995142644 2117 52-53 -0.3561636603446594 2117 54-55 -0.48164843190494366 2117 56-57 -0.034232030581986095 2117 58-59 -0.98057602390341 2117 60-61 -0.912096783255194 2117 62-63 -0.6431589142608019 2117 64-65 -0.8902089985321631 2117 66-67 -0.4010235013533432 2117 68-69 -0.3068437442939498 2117 70-71 -0.5047036453052982 2117 72-73 -1.7695645384359757 2117 74-75 -0.9396637358654267 2117 76 -1.0435673192729027 2118 1 0.49248069883184087 2118 2 0.8881495256100784 2118 3 0.5513238212598317 2118 4 0.1039298222759335 2118 5 1.3083982254621311 2118 6 1.1536045028026152 2118 7 0.5109506769664982 2118 8 0.46839346435692875 2118 9 0.7871788230380403 2118 10-11 -0.5318197896725962 2118 12-13 0.4230210991417849 2118 14-15 0.03667581957286359 2118 16-17 0.3325554682253866 2118 18-19 0.9058364606965235 2118 20-21 -0.06856852350941622 2118 22-23 0.22674834956054113 2118 24-25 0.33283901718595743 2118 26-27 0.7568629605075863 2118 28-29 0.4665447654062653 2118 30-31 -0.17758141592346277 2118 32-33 0.38091216393235783 2118 34-35 0.03082042340425417 2118 36-37 -0.11651162915823221 2118 38-39 -0.007593015717660023 2118 40-41 0.30580541414927254 2118 42-43 0.1998336857561398 2118 44-45 -0.0894037551475968 2118 46-47 0.13919037232776432 2118 48-49 0.2825197554704033 2118 50-51 -0.08482362024995993 2118 52-53 -0.09650420829902728 2118 54-55 0.3030837796496044 2118 56-57 0.39414585052730544 2118 58-59 -0.09846315466980826 2118 60-61 -0.027324428523314737 2118 62-63 0.39052790205071375 2118 64-65 0.2819216006047611 2118 66-67 0.634703926472568 2118 68-69 -0.43918241890004595 2118 70-71 0.8287523477365504 2118 72-73 0.2807482438207387 2118 74-75 -0.016153215007701505 2118 76 0.1259037943657404 2119 1 -1.7208948756006883 2119 2 -1.4101376210268803 2119 3 -0.1565859406117731 2119 4 -1.150874522522841 2119 5 -0.9167445123181253 2119 6 -1.5103002458396375 2119 7 -1.7545415943049143 2119 8 -1.4678619318274464 2119 9 -1.4990974616019805 2119 10-11 -1.6237812156578357 2119 12-13 -1.7689089941599825 2119 14-15 -1.6083422837553627 2119 16-17 -0.43246681279397237 2119 18-19 -1.0739191443724074 2119 20-21 -1.8239647078848513 2119 22-23 -1.6817950287990193 2119 24-25 -1.6576741425411008 2119 26-27 -0.8389802368186885 2119 28-29 -1.08076758663384 2119 30-31 -1.8333918880025593 2119 32-33 -1.8625079725388858 2119 34-35 -1.460667530919654 2119 36-37 -2.0154289108738723 2119 38-39 -2.3967449444184012 2119 40-41 -1.3479684335042599 2119 42-43 -1.554552889929699 2119 44-45 -1.4944657242326826 2119 46-47 -1.6131073577933925 2119 48-49 -1.288901355795435 2119 50-51 -1.915748978835115 2119 52-53 -1.2391375462742396 2119 54-55 -1.3579313992948343 2119 56-57 -1.3446614186552601 2119 58-59 -0.8157553145361227 2119 60-61 -0.5604500377699395 2119 62-63 -1.6187500536657637 2119 64-65 -1.8506156504744773 2119 66-67 -1.8429976471244203 2119 68-69 -1.7286964141653307 2119 70-71 -1.3119662151377014 2119 72-73 -1.3408747891830188 2119 74-75 -0.9003789495850576 2119 76 -0.10359070500760481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 20.0 13 257.0 14 1061.0 15 1792.0 16 2357.0 17 2948.0 18 2994.0 19 2604.0 20 2078.0 21 1526.0 22 1101.0 23 810.0 24 746.0 25 660.0 26 699.0 27 762.0 28 864.0 29 965.0 30 1133.0 31 1391.0 32 1743.0 33 2301.0 34 2939.0 35 4279.0 36 7759.0 37 22307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.75387687969925 23.195195018796994 11.156308740601503 30.894619360902254 2 18.904194078947366 23.114426691729324 29.205827067669173 28.775552161654133 3 18.6281132518797 19.824953007518797 28.593456296992482 32.953477443609025 4 15.633811090225564 18.99964755639098 24.78559680451128 40.58094454887218 5 17.023026315789476 29.55386513157895 29.947427161654133 23.475681390977442 6 29.771205357142854 30.434974154135336 15.774788533834586 24.019031954887218 7 27.960526315789476 23.004288063909776 16.801280545112782 32.23390507518797 8 23.679804981203006 27.417175751879697 20.431449718045116 28.471569548872182 9 21.888216635338345 17.315260808270676 18.208117951127818 42.58840460526316 10-11 26.685855263157894 21.02546405075188 22.63422227443609 29.654458411654133 12-13 24.662975798872182 18.338815789473685 23.121035009398497 33.877173402255636 14-15 24.656367481203006 19.41890859962406 20.680363016917294 35.244360902255636 16-17 26.449424342105267 20.573895676691727 20.572427161654137 32.40425281954887 18-19 25.570518092105267 19.417440084586467 21.483640742481203 33.52840108082707 20-21 25.620447603383457 19.938028665413533 21.641506109022558 32.80001762218045 22-23 27.15210878759399 19.913798167293233 20.22732612781955 32.70676691729324 24-25 26.011806860902254 21.10549812030075 19.42111137218045 33.461583646616546 26-27 25.190172697368425 21.507871240601503 21.228853383458645 32.07310267857143 28-29 25.885514567669173 20.574629934210524 22.579152960526315 30.96070253759399 30-31 26.186560150375936 19.709674577067666 22.415413533834585 31.688351738721803 32-33 24.51906132518797 20.963052161654137 22.720864661654137 31.79702185150376 34-35 24.988986137218046 20.933681860902258 22.542440084586467 31.534891917293233 36-37 25.274612312030076 19.107583411654137 23.84060737781955 31.77719689849624 38-39 23.77525845864662 19.06499647556391 26.42299107142857 30.736753994360903 40-41 23.66658834586466 19.19349154135338 25.900933975563913 31.238986137218046 42-43 22.295729558270676 19.234609962406015 26.0257577537594 32.44390272556391 44-45 21.202420112781954 18.849124765037594 28.075070488721803 31.873384633458645 46-47 21.746504934210524 19.946839755639097 27.496475563909772 30.8101797462406 48-49 21.709792058270676 20.6546640037594 26.52358435150376 31.111959586466163 50-51 21.512276785714285 21.767064144736842 25.320870535714285 31.399788533834588 52-53 20.279458411654137 21.847832471804512 25.558035714285715 32.314673402255636 54-55 20.68403430451128 20.899171757518797 25.2437734962406 33.17302044172932 56-57 20.838228383458645 21.421963110902258 24.812764332706767 32.92704417293233 58-59 21.148819313909776 21.430774201127818 24.430216165413533 32.99019031954887 60-61 21.151022086466163 19.847714990601503 25.328213110902254 33.67304981203007 62-63 20.904311560150376 20.794907189849624 24.40451715225564 33.896264097744364 64-65 21.658394031954884 21.20388862781955 23.519002584586467 33.6187147556391 66-67 21.085673167293233 22.732612781954884 21.43003994360902 34.751674107142854 68-69 21.28392269736842 23.262746710526315 21.709792058270676 33.74353853383459 70-71 21.88013980263158 23.745888157894736 22.255345394736842 32.11862664473684 72-73 21.75531602443609 23.553512687969924 21.654722744360903 33.036448543233085 74-75 21.47703242481203 24.064555921052634 21.492451832706767 32.96595982142857 76 20.930744830827066 24.679863721804512 20.75746005639098 33.63193139097744 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.0 15 1.0 16 3.0 17 5.0 18 4.5 19 6.5 20 19.5 21 30.0 22 40.5 23 57.0 24 77.0 25 91.0 26 114.0 27 164.5 28 226.5 29 261.0 30 319.5 31 400.5 32 508.5 33 594.0 34 689.5 35 916.5 36 1144.5 37 1241.0 38 1336.5 39 1627.0 40 1941.0 41 2129.0 42 2198.0 43 2309.5 44 2484.0 45 2570.0 46 2593.0 47 2568.5 48 2426.0 49 2225.0 50 2142.0 51 2024.5 52 1772.5 53 1563.5 54 1489.0 55 1488.5 56 1469.0 57 1511.0 58 1572.0 59 1675.0 60 1915.5 61 2095.5 62 2138.0 63 2276.0 64 2422.5 65 2525.0 66 2600.0 67 2581.0 68 2620.5 69 2618.0 70 2460.0 71 2344.0 72 2227.5 73 1935.5 74 1613.5 75 1467.0 76 1235.5 77 829.0 78 478.0 79 302.0 80 223.5 81 97.5 82 32.5 83 15.0 84 9.0 85 5.0 86 7.0 87 7.0 88 4.5 89 2.0 90 1.5 91 4.0 92 7.0 93 4.5 94 1.5 95 2.5 96 4.0 97 2.5 98 1.5 99 15.0 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 68096.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.14420817669173 #Duplication Level Percentage of deduplicated Percentage of total 1 83.54374713696747 66.95547462406014 2 11.444800732936326 18.34468984962406 3 3.3055428309665595 7.947603383458647 4 0.978469995419148 3.1367481203007515 5 0.3811268896014659 1.5272556390977443 6 0.15574896930829132 0.7489426691729324 7 0.08795235913879981 0.4934210526315789 8 0.03298213467704993 0.21146616541353383 9 0.02382043060009162 0.17181625939849623 >10 0.04580852038479157 0.4625822368421052 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00881109022556391 0.0 2 0.0 0.0 0.0 0.011748120300751879 0.0 3 0.0 0.0 0.0 0.016153665413533833 0.0 4 0.0 0.0 0.0 0.022027725563909775 0.0 5 0.0 0.0 0.0 0.04258693609022556 0.0 6 0.0 0.0 0.0 0.04258693609022556 0.0 7 0.0 0.0 0.0 0.04405545112781955 0.0 8 0.0 0.0 0.0 0.046992481203007516 0.0 9 0.0 0.0 0.0 0.05286654135338346 0.0 10 0.0 0.0 0.0 0.05727208646616541 0.0 11 0.0 0.0 0.0 0.05727208646616541 0.0 12 0.0 0.0 0.0 0.0587406015037594 0.0 13 0.0 0.0 0.0 0.0587406015037594 0.0 14 0.0 0.0 0.0 0.06608317669172932 0.0 15 0.0 0.0 0.0 0.07048872180451128 0.0 16 0.0 0.0 0.0 0.07048872180451128 0.0 17 0.0 0.0 0.0 0.0778312969924812 0.0 18 0.0 0.0 0.0 0.08076832706766918 0.0 19 0.0 0.0 0.0 0.08664238721804511 0.0 20 0.0 0.0 0.0 0.10279605263157894 0.0 21 0.0 0.0 0.0 0.1072015977443609 0.0 22 0.0 0.0 0.0 0.11454417293233082 0.0 23 0.0 0.0 0.0 0.12335526315789473 0.0 24 0.0 0.0 0.0 0.12776080827067668 0.0 25 0.0 0.0 0.0 0.14538298872180452 0.0 26 0.0 0.0 0.0 0.17181625939849623 0.0 27 0.0 0.0 0.0 0.2628641917293233 0.0 28 0.0 0.0 0.0 0.39796757518796994 0.0 29 0.0 0.0 0.0 0.5095747180451128 0.0 30 0.0 0.0 0.0 0.6637687969924813 0.0 31 0.0 0.0 0.0 0.873766447368421 0.0 32 0.0 0.0 0.0 1.058799342105263 0.0 33 0.0 0.0 0.0 1.356907894736842 0.0 34 0.0 0.0 0.0 1.7578125 0.0 35 0.0 0.0 0.0 2.35109257518797 0.0 36 0.0 0.0 0.0 3.045700187969925 0.0 37 0.0 0.0 0.0 3.8798167293233083 0.0 38 0.0 0.0 0.0 4.728618421052632 0.0 39 0.0 0.0 0.0 5.493714755639098 0.0 40 0.0 0.0 0.0 6.324894266917293 0.0 41 0.0 0.0 0.0 7.1781015037593985 0.0 42 0.0 0.0 0.0 8.018092105263158 0.0 43 0.0 0.0 0.0 8.856614191729323 0.0 44 0.0 0.0 0.0 9.673108552631579 0.0 45 0.0 0.0 0.0 10.513099154135338 0.0 46 0.0 0.0 0.0 11.279664003759398 0.0 47 0.0 0.0 0.0 11.978677161654135 0.0 48 0.0 0.0 0.0 12.630697838345865 0.0 49 0.0 0.0 0.0 13.278312969924812 0.0 50 0.0 0.0 0.0 13.858376409774436 0.0 51 0.0 0.0 0.0 14.458999060150376 0.0 52 0.0 0.0 0.0 15.118362312030076 0.0 53 0.0 0.0 0.0 15.786536654135338 0.0 54 0.0 0.0 0.0 16.444431390977442 0.0 55 0.0 0.0 0.0 17.137570488721803 0.0 56 0.0 0.0 0.0 17.694137687969924 0.0 57 0.0 0.0 0.0 18.17140507518797 0.0 58 0.0 0.0 0.0 18.733846334586467 0.0 59 0.0 0.0 0.0 19.29481907894737 0.0 60 0.0 0.0 0.0 19.882225093984964 0.0 61 0.0 0.0 0.0 20.47403665413534 0.0 62 0.0 0.0 0.0 21.026198308270676 0.0 63 0.0 0.0 0.0 21.56514332706767 0.0 64 0.0 0.0 0.0 22.12464755639098 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGAGTC 15 0.0022158956 70.00001 37 ATTTGCC 15 0.0022158956 70.00001 26 AGAGGCC 20 0.006924106 52.5 8 GACTCTT 20 0.006924106 52.5 14 GTTGCGG 20 0.006924106 52.5 22 ATCTCTG 40 0.0024035284 35.0 42 TTATATC 40 0.0024035284 35.0 38 GTAGATC 80 6.104892E-8 35.0 38 GATCTCT 45 0.004280311 31.111109 41 TAGATCT 105 1.9679646E-8 30.000002 39 TTATACC 95 2.7591523E-7 29.473686 2 CATCTTA 60 4.9357646E-4 29.166668 9 TGTAGAT 100 4.3224463E-7 28.000002 37 ATATCTC 65 7.8822306E-4 26.923079 40 TTTATAT 65 7.8822306E-4 26.923079 37 AGATCTC 105 6.6206485E-7 26.666668 40 TATACCC 105 6.6206485E-7 26.666668 3 TATATCT 80 8.553635E-5 26.25 39 ACTCTTC 85 1.2914263E-4 24.705883 15 ATTATAC 85 1.2914263E-4 24.705883 1 >>END_MODULE