FastQCFastQC Report
Wed 25 May 2016
SRR1780170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68096
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT948513.928865131578947No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG24403.5831766917293235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC23123.3952067669172936No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG16782.464168233082707Illumina PCR Primer Index 10 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6230.9148848684210527No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3140.46111372180451127Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1950.2863604323308271No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1720.2525845864661654No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1700.24964755639097747No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1610.23643092105263158No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT1150.16887922932330826No Hit
CTTATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC930.1365718984962406No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGC780.11454417293233082No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT750.11013862781954888Illumina PCR Primer Index 10 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC750.11013862781954888Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCT150.002215895670.0000134
GATTACC150.002215895670.0000133
GCACTTT150.002215895670.0000122
TCTTTAT800.070.01
TATATAC200.00692410652.53
TTATATA200.00692410652.52
ATCGTAT200.00692410652.539
CTTATAT200.00692410652.51
ATATACA200.00692410652.54
TACCTCG200.00692410652.536
CTTTATA1100.050.909092
CTTAATA352.066642E-550.01
TTAATAC352.066642E-550.02
AGATTAC305.840282E-446.66666832
TAATACA451.6623544E-646.6666643
TTTATAC1250.044.83
AAAGAAA350.001247463340.070
AAAAGAA556.6068387E-638.18181669
GCCGTCT20300.036.55172346
TATGCCG20500.036.53658743
Produced by FastQC (version 0.11.5)