##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68096 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73650434680451 34.0 34.0 34.0 34.0 34.0 2 33.73127643327068 34.0 34.0 34.0 34.0 34.0 3 33.740484022556394 34.0 34.0 34.0 34.0 34.0 4 33.75271675281955 34.0 34.0 34.0 34.0 34.0 5 33.74070429981203 34.0 34.0 34.0 34.0 34.0 6 37.51029429041353 38.0 38.0 38.0 37.0 38.0 7 37.493391682330824 38.0 38.0 38.0 37.0 38.0 8 37.408394031954884 38.0 38.0 38.0 37.0 38.0 9 37.44046640037594 38.0 38.0 38.0 37.0 38.0 10-11 37.37499265742481 38.0 38.0 38.0 37.0 38.0 12-13 37.36641652960526 38.0 38.0 38.0 37.0 38.0 14-15 37.273833999060145 38.0 38.0 38.0 36.0 38.0 16-17 37.285449953007515 38.0 38.0 38.0 36.0 38.0 18-19 37.18839579417293 38.0 38.0 38.0 36.0 38.0 20-21 37.233890390037594 38.0 38.0 38.0 36.0 38.0 22-23 37.2541485549812 38.0 38.0 38.0 36.0 38.0 24-25 37.256079652255636 38.0 38.0 38.0 36.0 38.0 26-27 37.10853794642857 38.0 38.0 38.0 35.5 38.0 28-29 37.06522409539474 38.0 38.0 38.0 36.0 38.0 30-31 37.084109198778194 38.0 38.0 38.0 36.0 38.0 32-33 37.01757078242481 38.0 38.0 38.0 36.0 38.0 34-35 36.9059489544173 38.0 37.5 38.0 35.0 38.0 36-37 36.81400522791354 38.0 37.0 38.0 34.5 38.0 38-39 35.9768635455827 38.0 37.0 38.0 32.5 38.0 40-41 36.53982612781955 38.0 37.0 38.0 34.0 38.0 42-43 36.914319490131575 38.0 37.0 38.0 35.0 38.0 44-45 37.020764802631575 38.0 38.0 38.0 35.5 38.0 46-47 36.954226386278194 38.0 38.0 38.0 35.5 38.0 48-49 36.97492510573308 38.0 38.0 38.0 35.5 38.0 50-51 36.72941876174812 38.0 37.0 38.0 34.0 38.0 52-53 36.68073748825188 38.0 37.0 38.0 34.0 38.0 54-55 36.713779076597746 38.0 37.0 38.0 34.5 38.0 56-57 36.7565348919173 38.0 37.0 38.0 34.5 38.0 58-59 36.56703771146616 38.0 37.0 38.0 34.0 38.0 60-61 36.55700775375939 38.0 37.0 38.0 34.0 38.0 62-63 36.562375176221806 38.0 37.0 38.0 34.0 38.0 64-65 36.480013510338345 38.0 37.0 38.0 34.0 38.0 66-67 35.988501527255636 38.0 37.0 38.0 32.5 38.0 68-69 35.76765889332707 38.0 37.0 38.0 31.0 38.0 70-71 34.43921816259399 38.0 35.5 38.0 25.0 38.0 72-73 32.05968045112782 38.0 28.0 38.0 11.0 38.0 74-75 31.380697838345863 38.0 25.0 38.0 11.0 38.0 76 30.67484140037594 37.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.047029311988211475 1101 2 -0.24080395585034609 1101 3 -0.38557719450935934 1101 4 -0.19092297963003801 1101 5 -0.13356468935180743 1101 6 -0.04916117722284241 1101 7 0.0991707237577657 1101 8 0.0018827532514080758 1101 9 -0.0821090483905067 1101 10-11 -0.2530729947931718 1101 12-13 -0.05396755303680578 1101 14-15 0.1983376950875524 1101 16-17 -0.10700717785697123 1101 18-19 -0.12822040812044122 1101 20-21 -0.11372621957062279 1101 22-23 0.09476440182488943 1101 24-25 0.1033142000700451 1101 26-27 0.07989464900329324 1101 28-29 -0.2351649415776791 1101 30-31 -0.13652771650106388 1101 32-33 -0.03249726891923643 1101 34-35 0.35389928678407756 1101 36-37 0.18760305488383722 1101 38-39 -0.1399681079352959 1101 40-41 0.12859334161444735 1101 42-43 -0.17801261186875905 1101 44-45 0.13753882803522544 1101 46-47 -0.14627777320070123 1101 48-49 -0.1520368127998779 1101 50-51 -0.12965947046782134 1101 52-53 -0.10777046088774256 1101 54-55 -0.13400103445933098 1101 56-57 -0.19420739120734964 1101 58-59 -0.17338287817234743 1101 60-61 0.058743251734867385 1101 62-63 -0.15604809378628914 1101 64-65 0.20530797770796738 1101 66-67 0.013262752553877988 1101 68-69 -0.7349301184016923 1101 70-71 -0.43950074175645426 1101 72-73 -0.5406913436521776 1101 74-75 -0.3393576436053536 1101 76 -1.0578172735695404 1102 1 -0.27237543780798035 1102 2 -0.20540196410878764 1102 3 0.10968634690429013 1102 4 -0.25486517054575586 1102 5 -0.3403293237943643 1102 6 0.008131754523574841 1102 7 -0.319246813283776 1102 8 -0.368198616678157 1102 9 -0.8304998669477044 1102 10-11 -0.2786832692648744 1102 12-13 0.0782282205847693 1102 14-15 -0.2907927396950569 1102 16-17 -0.036613080213058424 1102 18-19 -0.02873959685009453 1102 20-21 -0.16862056010985782 1102 22-23 -0.11324507109227966 1102 24-25 -0.4176507015573492 1102 26-27 -0.28630039593233647 1102 28-29 -0.10923890320022167 1102 30-31 -0.32553063124480985 1102 32-33 -0.16300795631296694 1102 34-35 0.2217642369905377 1102 36-37 -0.11773153340861597 1102 38-39 -0.16629188738635037 1102 40-41 0.18918054103149018 1102 42-43 0.005525158352277515 1102 44-45 0.03937876002454033 1102 46-47 -0.19561588347517045 1102 48-49 -0.023241573547991834 1102 50-51 -0.08657591448045565 1102 52-53 0.2256337169116236 1102 54-55 -0.13861604538962524 1102 56-57 -0.3497059338354802 1102 58-59 -0.03311083542277515 1102 60-61 -0.05057481967635624 1102 62-63 0.0065551367213601 1102 64-65 -0.20398580417204215 1102 66-67 -0.031202270521184516 1102 68-69 -0.42130672758312926 1102 70-71 -0.39678152873726447 1102 72-73 -0.08221126106777632 1102 74-75 0.04130485335845435 1102 76 0.056677504181248395 1103 1 0.21132021436593362 1103 2 0.07177194893468908 1103 3 0.13686025994776685 1103 4 0.10383048162815811 1103 5 0.19227937185780775 1103 6 -0.14947694112859722 1103 7 0.19705753454231711 1103 8 -0.0421116601564151 1103 9 -0.23810856259988356 1103 10-11 0.09631673073512559 1103 12-13 -0.025032648980449324 1103 14-15 0.1630116081310362 1103 16-17 -0.12356960195219102 1103 18-19 0.009303881410772874 1103 20-21 -0.11427273402289728 1103 22-23 0.09599405934250171 1103 24-25 0.025284081051339058 1103 26-27 0.14576482145896108 1103 28-29 -0.13097803363500304 1103 30-31 -0.1923784573317704 1103 32-33 0.290796391513112 1103 34-35 0.07230771525140511 1103 36-37 0.07248585789572815 1103 38-39 -0.7722701482559131 1103 40-41 -0.19125424157719806 1103 42-43 -0.24175745034337126 1103 44-45 -0.0013821095406783002 1103 46-47 -0.005398492170819225 1103 48-49 0.02838886123461748 1103 50-51 0.03570669421520023 1103 52-53 -0.4211054135231578 1103 54-55 -0.11959430625918799 1103 56-57 -0.6622059338354802 1103 58-59 0.05112829501200622 1103 60-61 -0.0967704718502631 1103 62-63 -0.18366225458299112 1103 64-65 -0.2121379780850816 1103 66-67 -0.6507674879124821 1103 68-69 -0.26913281453965254 1103 70-71 -0.3369989200416157 1103 72-73 -0.7180808262851635 1103 74-75 -0.964129929250241 1103 76 -1.0465833653839667 1104 1 0.07655978845643574 1104 2 -0.11045963935279701 1104 3 0.08705990502318883 1104 4 0.09273908854918744 1104 5 -0.13025833887865446 1104 6 -0.061189452219991836 1104 7 -0.10573749651358355 1104 8 -0.08037696627886248 1104 9 0.0056802572758982706 1104 10-11 0.022836251586952017 1104 12-13 -0.10344879804876683 1104 14-15 0.06823565427122702 1104 16-17 0.043189492990137524 1104 18-19 -0.21834696153510436 1104 20-21 -0.04251142080195791 1104 22-23 -0.11457603826175955 1104 24-25 -0.140643159410061 1104 26-27 -0.04761361687288712 1104 28-29 -0.07557652520554115 1104 30-31 -0.18112069335661118 1104 32-33 -0.22406607521270416 1104 34-35 -0.2760174843936696 1104 36-37 -0.5502404065230735 1104 38-39 -0.5571525794892835 1104 40-41 -0.27904261779725914 1104 42-43 -0.32688389222447256 1104 44-45 -0.107914940064191 1104 46-47 -0.32416512926044305 1104 48-49 -0.30063731445304853 1104 50-51 -0.5860213448265057 1104 52-53 -0.319619166850579 1104 54-55 -0.6936293550613186 1104 56-57 -0.8696149844122303 1104 58-59 -0.3787186437635057 1104 60-61 -0.530998510891969 1104 62-63 -0.42173900702309197 1104 64-65 -0.3980297260886303 1104 66-67 -0.20417254558773124 1104 68-69 -0.1405281117889885 1104 70-71 -0.4189643148952058 1104 72-73 0.1813756954539656 1104 74-75 0.044022244662802024 1104 76 -0.1291033298915103 1105 1 -0.13870291236857213 1105 2 -0.003968106569473662 1105 3 -0.1854107853806326 1105 4 -0.2812296478815597 1105 5 -0.07229047088039664 1105 6 -0.1046110761887249 1105 7 0.049277701054805334 1105 8 -0.26285634100747757 1105 9 0.1834918074278704 1105 10-11 0.035319968477963926 1105 12-13 0.12679436304545533 1105 14-15 0.030784503598191293 1105 16-17 -0.12767459732684472 1105 18-19 0.10129046790476082 1105 20-21 0.2502046017772841 1105 22-23 0.1894838835793209 1105 24-25 0.011061139330713843 1105 26-27 0.14593827196775067 1105 28-29 0.15955156858516517 1105 30-31 -0.08917540460281259 1105 32-33 0.09236900945946047 1105 34-35 -0.23579820519262285 1105 36-37 0.257673184445224 1105 38-39 0.30514659549986334 1105 40-41 0.13107462058745512 1105 42-43 -0.09403543369212031 1105 44-45 0.06886534651852827 1105 46-47 0.08063296018810462 1105 48-49 -0.09215924237315676 1105 50-51 0.11219836859077503 1105 52-53 0.3960777502141255 1105 54-55 0.2270176271358153 1105 56-57 0.230254750715865 1105 58-59 0.09501127373540896 1105 60-61 0.39860418124871444 1105 62-63 0.21232525698037819 1105 64-65 0.3065946768640373 1105 66-67 -0.8421990327783462 1105 68-69 -0.006355293725590627 1105 70-71 -0.09775285158647762 1105 72-73 0.5218012273688615 1105 74-75 0.5280647978542881 1105 76 0.9406969306382322 1106 1 0.09260320004072042 1106 2 0.059677201510716316 1106 3 -0.0026534369610047293 1106 4 -0.021452252727208077 1106 5 0.04362393079221505 1106 6 0.043033739947048844 1106 7 -0.024606200064368977 1106 8 0.1378016346412707 1106 9 0.12046142986053354 1106 10-11 0.24608740175799682 1106 12-13 0.13973865034098765 1106 14-15 0.024927290463274687 1106 16-17 0.13436266746223424 1106 18-19 0.2257370304431916 1106 20-21 0.2083585803777055 1106 22-23 0.13885972910877342 1106 24-25 0.1768925253840905 1106 26-27 0.10044878727952522 1106 28-29 0.014520207129010032 1106 30-31 0.13648872035106763 1106 32-33 0.16702157371970117 1106 34-35 -0.14277459186093466 1106 36-37 0.20331332710910033 1106 38-39 0.5433399296531007 1106 40-41 0.5738092165781126 1106 42-43 0.25825825712081496 1106 44-45 0.19114427899915398 1106 46-47 0.22305086696464116 1106 48-49 0.2910961997618813 1106 50-51 -0.16325975464129527 1106 52-53 0.3672836289498278 1106 54-55 0.504259048855161 1106 56-57 0.4741976851642704 1106 58-59 0.46550690772876635 1106 60-61 0.46473995790595524 1106 62-63 0.23988951721897678 1106 64-65 0.24849911666234448 1106 66-67 0.49140077044388164 1106 68-69 0.17172858279636216 1106 70-71 0.7811212106444856 1106 72-73 0.7724161578817075 1106 74-75 1.0013921868593378 1106 76 0.43073505633002895 1107 1 0.16448857699312924 1107 2 0.2247552976858458 1107 3 0.07522751249170767 1107 4 0.1389831180758918 1107 5 -0.06697132194422295 1107 6 0.21453785998149044 1107 7 0.06523514786331219 1107 8 0.27969685048188353 1107 9 0.21008755210872465 1107 10-11 0.27468167060561655 1107 12-13 0.08158159708800383 1107 14-15 0.15844407806627459 1107 16-17 0.21807008352422486 1107 18-19 -0.09285846826545452 1107 20-21 -0.2976677386482436 1107 22-23 -0.0658352653568528 1107 24-25 0.2743590116711374 1107 26-27 0.4757255060103063 1107 28-29 0.37763667496814435 1107 30-31 0.09965438263122905 1107 32-33 0.12960305201265498 1107 34-35 0.2402656245946062 1107 36-37 0.48905616316862677 1107 38-39 0.5312104252870995 1107 40-41 0.07522355675767045 1107 42-43 0.267666693967449 1107 44-45 0.13003555928448662 1107 46-47 0.24020742827320873 1107 48-49 0.3333726725204613 1107 50-51 0.08028347497375421 1107 52-53 0.20724796298007675 1107 54-55 0.607336776071989 1107 56-57 0.06270155922650389 1107 58-59 0.18277723118221445 1107 60-61 0.05019992592956868 1107 62-63 0.058069937831440654 1107 64-65 0.40765850665127346 1107 66-67 0.35992862679611193 1107 68-69 0.07343194031695788 1107 70-71 0.1841620420305361 1107 72-73 -0.06330515560986782 1107 74-75 -0.19002030852868756 1107 76 -0.048664771489427494 1108 1 0.09162860668140382 1108 2 0.017803293727411074 1108 3 0.2168653155594953 1108 4 0.1978648597879129 1108 5 0.15284560037146377 1108 6 0.1568931296499656 1108 7 0.05954489551299247 1108 8 0.1875395026342801 1108 9 0.10617859614632863 1108 10-11 -0.18041481628205958 1108 12-13 -0.0432328512049196 1108 14-15 0.16054699741312817 1108 16-17 0.14279794102051113 1108 18-19 0.06523158616305125 1108 20-21 0.10046437416718845 1108 22-23 0.08594603103107801 1108 24-25 -0.04435697051589926 1108 26-27 0.21205653025572957 1108 28-29 0.23612257303840067 1108 30-31 0.245374323254687 1108 32-33 -0.00622079756676186 1108 34-35 0.012841082288822747 1108 36-37 0.27919718246600667 1108 38-39 0.5436423896611728 1108 40-41 0.2372720476037813 1108 42-43 -0.0517296444788613 1108 44-45 0.2624576275675139 1108 46-47 0.3316844198615385 1108 48-49 0.16495357306475 1108 50-51 0.1482572503324846 1108 52-53 0.05336374724529236 1108 54-55 0.12174795865486487 1108 56-57 0.10321368193802982 1108 58-59 0.0351399229189866 1108 60-61 0.11607078194144549 1108 62-63 0.009146137732479076 1108 64-65 0.4619014556864016 1108 66-67 0.703694089438379 1108 68-69 0.8779222916281952 1108 70-71 1.3843104833962059 1108 72-73 1.7191663931283898 1108 74-75 2.0323943376860605 1108 76 2.047451012775788 1109 1 0.09083435074036572 1109 2 -0.06587951221997912 1109 3 -0.016709471579908097 1109 4 0.04835412144758777 1109 5 0.15367162655013544 1109 6 -0.15826762775249392 1109 7 0.13528514205003717 1109 8 -0.14935209731488186 1109 9 0.11809243526184332 1109 10-11 -0.06493000245565383 1109 12-13 0.0070117796249178355 1109 14-15 -0.1951049278632624 1109 16-17 0.027757910521081897 1109 18-19 0.10589749034400597 1109 20-21 0.006599207055742795 1109 22-23 0.06788465093195839 1109 24-25 0.16896429012713554 1109 26-27 0.05965470029004649 1109 28-29 0.013667369073480984 1109 30-31 -0.0044032850818211955 1109 32-33 0.13684058253235776 1109 34-35 -0.113588578405043 1109 36-37 -0.14105952105413166 1109 38-39 -0.18901106058663686 1109 40-41 -0.26857367612222305 1109 42-43 0.15814454538007539 1109 44-45 0.10557619409438956 1109 46-47 0.33042949547469647 1109 48-49 0.10238209003005494 1109 50-51 -0.21139772488673003 1109 52-53 -0.3652726741551433 1109 54-55 -0.2776039284972498 1109 56-57 0.19601454608849167 1109 58-59 -0.2845957487038362 1109 60-61 0.017805436916660256 1109 62-63 -0.4383694296839664 1109 64-65 0.17717658594437324 1109 66-67 0.3485346028454117 1109 68-69 0.2473942702785905 1109 70-71 0.4342056416119817 1109 72-73 0.18274181567254288 1109 74-75 0.8411533922037826 1109 76 1.1042244274409292 1110 1 0.0416540228715121 1110 2 0.09805491400953059 1110 3 0.13629599290096195 1110 4 0.20135958592846492 1110 5 0.1919229926703565 1110 6 -0.010726644145940156 1110 7 0.151678584672986 1110 8 0.2550194873845655 1110 9 0.24923997624544825 1110 10-11 0.12359458770828269 1110 12-13 0.17914292716590552 1110 14-15 0.1655508098416476 1110 16-17 0.11245736407299489 1110 18-19 0.3736570531855392 1110 20-21 0.2880199720830632 1110 22-23 0.3165185307133811 1110 24-25 0.311040792859373 1110 26-27 0.3957202740605297 1110 28-29 0.4016455111499795 1110 30-31 0.4436841466121635 1110 32-33 0.22154003608427075 1110 34-35 0.025755683890039904 1110 36-37 0.3343503150114415 1110 38-39 0.14432227274669174 1110 40-41 0.2751421708723143 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1.4875887132054473 2115 74-75 1.169022244662802 2115 76 1.397010666002707 2116 1 0.19046581598372825 2116 2 0.12826841000648415 2116 3 0.0017490364897270183 2116 4 0.06414392955535675 2116 5 -0.02738695776807276 2116 6 0.06386987383601195 2116 7 0.12931233737345593 2116 8 0.26312089798312144 2116 9 0.036411154285865166 2116 10-11 0.24400378836909908 2116 12-13 -0.06939564190259517 2116 14-15 0.052988857878247586 2116 16-17 -0.2031322915376279 2116 18-19 -0.12662887895322683 2116 20-21 0.039417862539281145 2116 22-23 0.10629486824038281 2116 24-25 0.11261977367014708 2116 26-27 0.2701960651394515 2116 28-29 0.3814714102477126 2116 30-31 0.07676967209189911 2116 32-33 0.05191873731696006 2116 34-35 0.3297015474051008 2116 36-37 0.32570881037298705 2116 38-39 0.35178192454489476 2116 40-41 0.08320228016192743 2116 42-43 0.06745640203276793 2116 44-45 0.29443437175356024 2116 46-47 0.2531960477685118 2116 48-49 0.17367450329730616 2116 50-51 0.32267585498365037 2116 52-53 -0.1007060201965686 2116 54-55 0.07814330749207699 2116 56-57 0.20495786798454674 2116 58-59 0.4770003880352647 2116 60-61 0.3253731075228359 2116 62-63 0.4364717191278302 2116 64-65 0.35143633940733565 2116 66-67 0.2792754847872132 2116 68-69 0.0523408962793539 2116 70-71 0.8552407159543378 2116 72-73 0.5738175559190779 2116 74-75 0.3957664307093154 2116 76 0.3730324081246259 2117 1 -0.18110752427871546 2117 2 -0.06761959830844688 2117 3 0.13397587811633827 2117 4 0.026954115342029183 2117 5 0.11783195869288221 2117 6 -0.015641624150212863 2117 7 0.1438634611879266 2117 8 -0.46739098976535587 2117 9 -0.06534927185418837 2117 10-11 -0.11340819032389504 2117 12-13 -0.2662580558301002 2117 14-15 0.047499706260737184 2117 16-17 -0.09024740132066711 2117 18-19 -0.066767894153962 2117 20-21 -0.23104465532481555 2117 22-23 -0.10355962052147305 2117 24-25 -0.07825004574001326 2117 26-27 -0.11284278116431068 2117 28-29 -0.6513660588961159 2117 30-31 -0.005242800299946282 2117 32-33 -0.33137995048348046 2117 34-35 -0.03436886964050956 2117 36-37 -0.1336259953954908 2117 38-39 0.0779089870367713 2117 40-41 0.30177769915634656 2117 42-43 0.04824437137146731 2117 44-45 -0.306595250177061 2117 46-47 0.04925295305628907 2117 48-49 0.14684586876437322 2117 50-51 -0.0737935486801149 2117 52-53 0.11010663408432464 2117 54-55 -0.1759004758001126 2117 56-57 -0.38415152528556007 2117 58-59 0.21767857434161897 2117 60-61 0.05036336346671533 2117 62-63 0.21546028610197965 2117 64-65 -0.12988755301828547 2117 66-67 -0.7074076385075827 2117 68-69 -0.5812988334854907 2117 70-71 -0.5987186188514215 2117 72-73 -0.6133170419762024 2117 74-75 -0.31701127489027314 2117 76 -0.33156029032931755 2118 1 -0.039167939760595516 2118 2 -0.44459748610826466 2118 3 -0.017363900427135093 2118 4 -0.08531297059782617 2118 5 -0.08980862605940132 2118 6 0.034069010446515335 2118 7 -0.04949284296354506 2118 8 0.1397745673884927 2118 9 0.1040549364889003 2118 10-11 0.05357070105535655 2118 12-13 0.029998592883650588 2118 14-15 0.1743855992791694 2118 16-17 -0.05602534264471615 2118 18-19 -0.2032546970844109 2118 20-21 0.07155127014268459 2118 22-23 0.06636310386215882 2118 24-25 0.14797348485242878 2118 26-27 0.005713402557638858 2118 28-29 -0.12803283707160062 2118 30-31 0.13570993110874952 2118 32-33 -0.10115882848167956 2118 34-35 0.10031149030998421 2118 36-37 -0.07924871643386666 2118 38-39 0.27275393401432524 2118 40-41 0.12586695082060828 2118 42-43 -0.1184334461777965 2118 44-45 -0.19750616577603353 2118 46-47 0.13388815193227543 2118 48-49 -0.14111452330065788 2118 50-51 -0.3130468855817341 2118 52-53 -0.3660416280506311 2118 54-55 -0.5182046941790048 2118 56-57 -0.19858968806614996 2118 58-59 -0.06825793253290158 2118 60-61 -0.501287524701084 2118 62-63 -0.32075220511669755 2118 64-65 -0.24712235715303166 2118 66-67 0.20748882554755 2118 68-69 0.06569210084695953 2118 70-71 -0.6440804092371337 2118 72-73 -0.41892370574363724 2118 74-75 0.6151300290939403 2118 76 0.12205761352777245 2119 1 0.0741867462162844 2119 2 0.011989340239040303 2119 3 -0.015692823975385295 2119 4 -0.023065372770226134 2119 5 -0.11459626009365564 2119 6 -0.1745022191872394 2119 7 -0.10324580216142465 2119 8 0.11777206077381663 2119 9 0.21082975893702383 2119 10-11 -0.11064737442159611 2119 12-13 0.043976451120663285 2119 14-15 -0.5516623049124476 2119 16-17 -0.357202058979496 2119 18-19 -0.01907073941834625 2119 20-21 -0.14953562583281155 2119 22-23 -0.07393768989915372 2119 24-25 -0.1431941798182237 2119 26-27 -0.06701323718613139 2119 28-29 -0.15050533393834087 2119 30-31 0.07676967209189911 2119 32-33 -0.4916859138458278 2119 34-35 -0.2546007781763038 2119 36-37 -0.049291189627012955 2119 38-39 -0.5203110987109199 2119 40-41 -0.2423791151869068 2119 42-43 -0.47905522587421245 2119 44-45 -0.2171935352231813 2119 46-47 -0.19157139409195878 2119 48-49 -0.1460929385631573 2119 50-51 -0.13371949385355464 2119 52-53 -0.05128741554540284 2119 54-55 -0.029414832042803596 2119 56-57 -0.3880653878294069 2119 58-59 -0.5375344956856694 2119 60-61 -0.33160363666320336 2119 62-63 -0.3745747925000771 2119 64-65 -0.793912497801962 2119 66-67 -0.2672361431197672 2119 68-69 -0.3604498013950632 2119 70-71 -0.746503470092172 2119 72-73 -1.4552522115227795 2119 74-75 -1.0984196158023174 2119 76 -1.7606885221079303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 2.0 23 7.0 24 23.0 25 39.0 26 80.0 27 177.0 28 288.0 29 476.0 30 731.0 31 1184.0 32 1784.0 33 2973.0 34 5044.0 35 8793.0 36 12066.0 37 34428.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.54963580827068 12.085878759398497 12.704123590225564 42.66036184210527 2 17.993714755639097 14.699835526315788 40.525140977443606 26.781308740601506 3 17.469454887218046 12.884750939849624 37.0844102443609 32.56138392857143 4 15.028782894736842 27.605145676691727 27.862135808270676 29.50393562030075 5 16.57953477443609 25.06167763157895 42.061207706766915 16.297579887218046 6 28.77261513157895 38.46481437969925 14.561795112781956 18.20077537593985 7 26.364250469924812 20.91018562030075 19.102443609022558 33.62312030075188 8 22.435972744360903 39.66018562030075 12.02126409774436 25.88257753759399 9 19.783834586466163 14.758576127819548 12.100563909774436 53.35702537593985 10-11 25.899465460526315 27.139626409774436 21.21343397556391 25.747474154135336 12-13 24.00434680451128 14.369419642857142 26.072750234962406 35.55348331766917 14-15 21.310355968045116 12.630697838345863 17.542880639097742 48.51606555451128 16-17 32.322750234962406 22.835408834586467 13.067581062030076 31.77425986842105 18-19 36.532982847744364 19.902050046992482 12.895030545112782 30.669936560150372 20-21 26.08376409774436 15.351121945488721 13.533834586466165 45.03127937030075 22-23 23.274494830827066 25.11087288533835 13.058769971804512 38.55586231203007 24-25 34.08937382518797 24.0476679981203 14.149876644736842 27.713081531954884 26-27 35.6687617481203 27.761542528195488 13.828271851503759 22.74142387218045 28-29 24.886924342105264 27.953183740601506 15.382695018796994 31.77719689849624 30-31 32.2500587406015 19.862400140977442 22.681949013157894 25.205592105263158 32-33 31.802895911654133 23.98452185150376 20.761131343984964 23.451450892857142 34-35 26.688058035714285 26.489808505639097 23.787740836466163 23.03439262218045 36-37 23.383899201127818 23.206943139097742 30.85717222744361 22.551985432330827 38-39 20.824277490601503 19.957853618421055 29.412153430451127 29.805715460526315 40-41 22.028459821428573 13.529429041353383 28.813733552631575 35.62837758458647 42-43 31.484962406015036 12.916324013157896 26.918614896616543 28.680098684210524 44-45 21.833147321428573 23.419143562030076 30.912241541353385 23.83546757518797 46-47 29.022262687969924 16.641212406015036 25.001468515037594 29.335056390977442 48-49 28.962787828947366 13.967780780075186 18.57671522556391 38.49271616541353 50-51 23.936060855263158 14.807037124060152 27.357700892857146 33.89920112781955 52-53 18.181684680451127 14.757107612781956 39.90983317669173 27.151374530075188 54-55 19.093632518796994 15.8203125 32.14652843045113 32.93952655075188 56-57 27.608816964285715 17.242569313909776 22.170905780075188 32.97770794172932 58-59 20.66641212406015 17.84906602443609 33.51885573308271 27.96566611842105 60-61 27.759339755639097 25.47653312969925 23.093133223684212 23.670993890977442 62-63 19.880022321428573 40.8937382518797 15.464931860902256 23.761307565789476 64-65 17.73672462406015 44.04664003759399 14.867246240601503 23.34938909774436 66-67 17.972421287593985 43.598008693609025 14.624941259398497 23.804628759398497 68-69 17.495888157894736 44.01139567669173 15.094131813909776 23.39858435150376 70-71 18.001791588345863 41.9914532424812 15.843808740601503 24.162946428571427 72-73 18.668497415413533 37.27384868421053 17.43567904135338 26.621974859022558 74-75 18.760279605263158 35.063733552631575 18.816817434210524 27.359169407894733 76 18.50356120126294 35.38438945590719 19.29803950363463 26.81400983919524 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5 20 4.5 21 6.0 22 7.0 23 10.0 24 15.5 25 19.0 26 27.5 27 42.0 28 78.0 29 108.0 30 117.5 31 154.0 32 208.5 33 236.0 34 291.5 35 393.0 36 571.0 37 703.0 38 2984.0 39 6150.5 40 6289.5 41 4518.5 42 3494.0 43 2889.0 44 2012.5 45 1622.0 46 1503.0 47 1503.0 48 1378.5 49 1179.5 50 1105.0 51 1064.5 52 943.0 53 872.0 54 882.0 55 913.0 56 1022.5 57 1161.0 58 1221.0 59 1320.0 60 1570.0 61 1742.5 62 1764.0 63 1911.5 64 2136.0 65 2290.0 66 2481.0 67 2595.0 68 2564.5 69 2597.5 70 2458.5 71 2256.0 72 2220.5 73 1974.5 74 1634.0 75 1504.0 76 1242.5 77 793.5 78 447.0 79 288.0 80 197.0 81 66.0 82 18.0 83 10.0 84 5.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0014685150375939849 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 68096.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.206943139097746 #Duplication Level Percentage of deduplicated Percentage of total 1 67.47494440551985 32.52760808270676 2 21.82045267615073 21.037946428571427 3 6.759679532092484 9.775904605263158 4 2.14762238401316 4.141212406015038 5 0.8803728638011392 2.1220042293233083 6 0.35032138178938066 1.0132753759398496 7 0.1736375544521278 0.5859375 8 0.11880464251987694 0.4581766917293233 9 0.0670180034727511 0.29076597744360905 >10 0.14926737137112742 1.2996358082706767 >50 0.024370183081000397 0.8062147556390977 >100 0.0182776373107503 1.6550164473684208 >500 0.0030462728851250497 0.9148848684210527 >1k 0.00913881865537515 9.442551691729323 >5k 0.0030462728851250497 13.928865131578947 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 9485 13.928865131578947 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 2440 3.5831766917293235 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 2312 3.3952067669172936 No Hit CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG 1678 2.464168233082707 Illumina PCR Primer Index 10 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 623 0.9148848684210527 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 314 0.46111372180451127 Illumina PCR Primer Index 10 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT 195 0.2863604323308271 No Hit CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 172 0.2525845864661654 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 170 0.24964755639097747 No Hit CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 161 0.23643092105263158 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT 115 0.16887922932330826 No Hit CTTATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 93 0.1365718984962406 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGC 78 0.11454417293233082 No Hit TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT 75 0.11013862781954888 Illumina PCR Primer Index 10 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 75 0.11013862781954888 Illumina PCR Primer Index 10 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010279605263157895 0.0 2 0.0 0.0 0.0 0.013216635338345864 0.0 3 0.0 0.0 0.0 0.019090695488721804 0.0 4 0.0 0.0 0.0 0.024964755639097745 0.0 5 0.0 0.0 0.0 0.045523966165413536 0.0 6 0.0 0.0 0.0 0.045523966165413536 0.0 7 0.0 0.0 0.0 0.046992481203007516 0.0 8 0.0 0.0 0.0 0.04992951127819549 0.0 9 0.0 0.0 0.0 0.05727208646616541 0.0 10 0.0 0.0 0.0 0.061677631578947366 0.0 11 0.0 0.0 0.0 0.06314614661654136 0.0 12 0.0 0.0 0.0 0.06461466165413533 0.0 13 0.0 0.0 0.0 0.06461466165413533 0.0 14 0.0 0.0 0.0 0.07048872180451128 0.0 15 0.0 0.0 0.0 0.07489426691729323 0.0 16 0.0 0.0 0.0 0.07489426691729323 0.0 17 0.0 0.0 0.0 0.08223684210526316 0.0 18 0.0 0.0 0.0 0.08517387218045112 0.0 19 0.0 0.0 0.0 0.09104793233082707 0.0 20 0.0 0.0 0.0 0.10867011278195489 0.0 21 0.0 0.0 0.0 0.10867011278195489 0.0 22 0.0 0.0 0.0 0.11454417293233082 0.0 23 0.0 0.0 0.0 0.12335526315789473 0.0 24 0.0 0.0 0.0 0.12776080827067668 0.0 25 0.0 0.0 0.0 0.14685150375939848 0.0 26 0.0 0.0 0.0 0.17328477443609022 0.0 27 0.0 0.0 0.0 0.2643327067669173 0.0 28 0.0 0.0 0.0 0.39796757518796994 0.0 29 0.0 0.0 0.0 0.5125117481203008 0.0 30 0.0 0.0 0.0 0.6740484022556391 0.0 31 0.0 0.0 0.0 0.8913886278195489 0.0 32 0.0 0.0 0.0 1.0911066729323309 0.0 33 0.0 0.0 0.0 1.4083059210526316 0.0 34 0.0 0.0 0.0 1.8194901315789473 0.0 35 0.0 0.0 0.0 2.428923872180451 0.0 36 0.0 0.0 0.0 3.1602443609022557 0.0 37 0.0 0.0 0.0 4.006109022556391 0.0 38 0.0 0.0 0.0 4.84609962406015 0.0 39 0.0 0.0 0.0 5.6141329887218046 0.0 40 0.0 0.0 0.0 6.454123590225564 0.0 41 0.0 0.0 0.0 7.320547462406015 0.0 42 0.0 0.0 0.0 8.169349154135338 0.0 43 0.0 0.0 0.0 9.007871240601503 0.0 44 0.0 0.0 0.0 9.837582236842104 0.0 45 0.0 0.0 0.0 10.686383928571429 0.0 46 0.0 0.0 0.0 11.470570958646617 0.0 47 0.0 0.0 0.0 12.193080357142858 0.0 48 0.0 0.0 0.0 12.856849154135338 0.0 49 0.0 0.0 0.0 13.508869830827068 0.0 50 0.0 0.0 0.0 14.10655545112782 0.0 51 0.0 0.0 0.0 14.727737312030076 0.0 52 0.0 0.0 0.0 15.382695018796992 0.0 53 0.0 0.0 0.0 16.055274906015036 0.0 54 0.0 0.0 0.0 16.729323308270676 0.0 55 0.0 0.0 0.0 17.435679041353385 0.0 56 0.0 0.0 0.0 18.021616541353385 0.0 57 0.0 0.0 0.0 18.51797462406015 0.0 58 0.0 0.0 0.0 19.070136278195488 0.0 59 0.0 0.0 0.0 19.642857142857142 0.0 60 0.0 0.0 0.0 20.225857612781954 0.0 61 0.0 0.0 0.0 20.816200657894736 0.0 62 0.0 0.0 0.0 21.37423637218045 0.0 63 0.0 0.0 0.0 21.91611842105263 0.0 64 0.0 0.0 0.0 22.48296522556391 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACCT 15 0.0022158956 70.00001 34 GATTACC 15 0.0022158956 70.00001 33 GCACTTT 15 0.0022158956 70.00001 22 TCTTTAT 80 0.0 70.0 1 TATATAC 20 0.006924106 52.5 3 TTATATA 20 0.006924106 52.5 2 ATCGTAT 20 0.006924106 52.5 39 CTTATAT 20 0.006924106 52.5 1 ATATACA 20 0.006924106 52.5 4 TACCTCG 20 0.006924106 52.5 36 CTTTATA 110 0.0 50.90909 2 CTTAATA 35 2.066642E-5 50.0 1 TTAATAC 35 2.066642E-5 50.0 2 AGATTAC 30 5.840282E-4 46.666668 32 TAATACA 45 1.6623544E-6 46.666664 3 TTTATAC 125 0.0 44.8 3 AAAGAAA 35 0.0012474633 40.0 70 AAAAGAA 55 6.6068387E-6 38.181816 69 GCCGTCT 2030 0.0 36.551723 46 TATGCCG 2050 0.0 36.536587 43 >>END_MODULE