FastQCFastQC Report
Wed 25 May 2016
SRR1780169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296584
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT801.2914825E-1030.23747325
GAAACTC752.1627784E-929.32118426
TTTACAC602.9651892E-729.2964652
GGTATAG300.005773489429.2964651
TATTACA300.005773489429.2964653
AATTAAA300.005773489429.2964655
AAACTCG804.307367E-927.48861127
AGTTGAT407.036542E-427.48861129
GCTTAAT407.0713274E-427.4654351
TATGCAG450.001399099524.4343230
CTTATTA450.00140599324.4137211
CTTTACA651.8250887E-523.6625291
CAGTTGA500.002582089321.99088728
TTACTAT500.00259476921.9723476
AGTGTAG654.8946694E-420.32328835
ACGAAAC1101.2518831E-720.00859824
GACGAAA1553.274181E-1119.87279723
TAGATCT1504.5656634E-1019.08130639
ACGAGAG708.0801704E-418.86524824
TGTAGAT1951.8189894E-1218.06514437
Produced by FastQC (version 0.11.5)