FastQCFastQC Report
Wed 25 May 2016
SRR1780169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296584
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT198486.692201871982305No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG52361.765435761875219No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC50031.6868745448169828No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG32231.0867073072047042RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC11800.3978636743721846No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT5520.18611927818088636No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC3220.10856957893885039No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG3100.10452350767404849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT302.4849815E-644.0929371
GCAAACT207.8502815E-443.9962927
ATCACTA207.8502815E-443.99629215
TCTTCTG3750.039.30335244
GTCTTCT4000.036.84689343
TTAGCAA250.00234508235.2148484
CTAGACA250.002350911535.19703319
TCACTAG250.002350911535.19703316
CATCGTA250.002350911535.19703324
ATGCCGT26250.034.5266144
TCTTTAT1350.034.2945061
CGTCTTC4500.032.75279642
CCGTCTT4900.029.63015741
ATTAGCA300.005726299229.3457053
TTATATA454.04765E-529.3457052
AGCAAAC300.005740479629.3308626
ACACGTA300.005740479629.33086238
TAGACAT300.005740479629.33086220
ATCGTAC300.005740479629.33086225
TAGCAAA300.005740479629.3308625
Produced by FastQC (version 0.11.5)