FastQCFastQC Report
Wed 25 May 2016
SRR1780168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268373
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACG308.362673E-658.33333234
TTAGACC200.006946260652.562
CTGTGTA200.006946260652.531
GGTACCC352.0831601E-550.046
TAACGGC352.0831601E-550.036
TAACCGT352.0831601E-550.053
TTAACGG352.0831601E-550.035
ATTAGAG459.183056E-538.8888853
GAACCTC459.183056E-538.88888517
GTGCAAA501.7069984E-435.058
GACACAT400.002416141335.026
CGCAATA501.7069984E-435.057
CAAAGGT501.7069984E-435.061
CAGTGAC652.0980518E-532.30769322
CGCGGTA552.9873874E-431.8181843
CGGTACC552.9873874E-431.8181845
CGATAGA552.9873874E-431.8181837
GATACTA450.004302635331.1111091
TGAGACC450.004302635331.11110919
CTCCGGT604.974238E-429.1666667