##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268373 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.163611838746817 34.0 33.0 34.0 21.0 34.0 2 31.05565015854799 34.0 33.0 34.0 21.0 34.0 3 30.858722747817403 34.0 32.0 34.0 21.0 34.0 4 30.67771348086432 34.0 32.0 34.0 21.0 34.0 5 30.75693531018396 34.0 32.0 34.0 21.0 34.0 6 33.786409214041655 38.0 34.0 38.0 21.0 38.0 7 34.21161592261517 38.0 34.0 38.0 23.0 38.0 8 34.11227657029582 38.0 34.0 38.0 23.0 38.0 9 34.433117340418 38.0 36.0 38.0 24.0 38.0 10-11 34.21089304810842 38.0 34.5 38.0 22.5 38.0 12-13 34.289865224892225 38.0 35.0 38.0 23.0 38.0 14-15 34.26535269941462 38.0 35.0 38.0 23.0 38.0 16-17 34.33971748275721 38.0 35.0 38.0 23.0 38.0 18-19 34.35412466976931 38.0 35.5 38.0 23.0 38.0 20-21 34.36436787605311 38.0 35.5 38.0 23.0 38.0 22-23 34.37964884694064 38.0 35.5 38.0 23.0 38.0 24-25 34.42326538064559 38.0 36.0 38.0 23.5 38.0 26-27 34.41586895850179 38.0 36.0 38.0 23.0 38.0 28-29 34.52306863954273 38.0 36.0 38.0 24.0 38.0 30-31 34.53104447913911 38.0 36.0 38.0 24.0 38.0 32-33 34.61505814668391 38.0 36.0 38.0 25.0 38.0 34-35 34.55181407965779 38.0 36.0 38.0 24.0 38.0 36-37 34.49130873821137 38.0 36.0 38.0 24.0 38.0 38-39 34.49235578839898 38.0 36.0 38.0 24.0 38.0 40-41 34.55461056067489 38.0 36.0 38.0 24.5 38.0 42-43 34.60709162248065 38.0 36.0 38.0 25.0 38.0 44-45 34.65915908083153 38.0 36.0 38.0 25.0 38.0 46-47 34.60084844600612 38.0 36.0 38.0 24.5 38.0 48-49 34.56419423712519 38.0 36.0 38.0 24.0 38.0 50-51 34.50935638085799 38.0 36.0 38.0 24.0 38.0 52-53 34.548978473989564 38.0 36.0 38.0 24.0 38.0 54-55 34.54464681618494 38.0 36.0 38.0 24.0 38.0 56-57 34.49379967433386 38.0 36.0 38.0 24.0 38.0 58-59 34.55166130721049 38.0 36.0 38.0 24.0 38.0 60-61 34.5575542249034 38.0 36.0 38.0 24.0 38.0 62-63 34.44148256344714 38.0 36.0 38.0 24.0 38.0 64-65 34.4124110845726 38.0 36.0 38.0 24.0 38.0 66-67 34.293636096030525 38.0 35.5 38.0 23.5 38.0 68-69 34.24190399183226 38.0 35.0 38.0 23.0 38.0 70-71 34.2121413107876 38.0 35.0 38.0 23.0 38.0 72-73 34.27748134126756 38.0 35.5 38.0 23.0 38.0 74-75 34.310925465676505 38.0 36.0 38.0 23.5 38.0 76 32.538396187395904 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.28845480130758006 1101 2 0.3599421152457438 1101 3 0.1566037410041652 1101 4 0.4986190890111395 1101 5 0.1437360447342826 1101 6 0.25120111804819345 1101 7 0.10010135859876357 1101 8 -0.3706997592415604 1101 9 -0.32228719615980594 1101 10-11 -0.5578449715716829 1101 12-13 -0.27236988665717377 1101 14-15 -0.03058323642093086 1101 16-17 -0.2914294325711353 1101 18-19 -0.22762461560450475 1101 20-21 -0.22571688791600053 1101 22-23 0.1921920884858892 1101 24-25 -0.17690955650051876 1101 26-27 -0.4013036693474561 1101 28-29 -0.4266822486567605 1101 30-31 -0.4149284950487697 1101 32-33 -0.33734743409672063 1101 34-35 -0.06923372796713068 1101 36-37 -0.4157856447923507 1101 38-39 -0.2484399663900092 1101 40-41 -0.008583273552197568 1101 42-43 -0.3635176838216694 1101 44-45 -0.39886536671130557 1101 46-47 -0.0800314560449138 1101 48-49 -0.3030643319274873 1101 50-51 -0.26256304637201566 1101 52-53 0.04791617041004059 1101 54-55 0.0010503855465842094 1101 56-57 0.19336562800134516 1101 58-59 -0.09381787091981408 1101 60-61 -0.13822088343348327 1101 62-63 -0.16588038935361737 1101 64-65 0.12394669066311081 1101 66-67 -0.21509257610683363 1101 68-69 -0.30187394616289254 1101 70-71 -0.25097918664626917 1101 72-73 -0.2603541748965199 1101 74-75 -0.3619333281472308 1101 76 -1.3208664312740623 1102 1 -0.1120971167417757 1102 2 -0.08274743195552503 1102 3 0.07297118503970523 1102 4 0.6417071478932712 1102 5 -0.24172437858271678 1102 6 0.08566424555159813 1102 7 -0.31837453754421574 1102 8 -0.07537210537741856 1102 9 -0.06214039746920008 1102 10-11 -0.08744259676737443 1102 12-13 0.22171546803856046 1102 14-15 -0.15617032952220455 1102 16-17 -0.022306971558180066 1102 18-19 -0.07095361139572987 1102 20-21 -0.05552088523214849 1102 22-23 0.14385831462093535 1102 24-25 0.024163985170375213 1102 26-27 0.0538017532579147 1102 28-29 0.19504375423041154 1102 30-31 0.1842033187893506 1102 32-33 0.07634022220729264 1102 34-35 -0.17249908388279067 1102 36-37 -0.13252516861156494 1102 38-39 0.005835901287845502 1102 40-41 -0.06579409843070749 1102 42-43 -0.17945916766771575 1102 44-45 0.08671704997738772 1102 46-47 -0.4136802777922668 1102 48-49 0.06427600924730825 1102 50-51 0.12348701157483077 1102 52-53 0.21309417907156813 1102 54-55 -0.17421037528540495 1102 56-57 0.041717700872048624 1102 58-59 -0.12878015463573433 1102 60-61 0.04100771181284557 1102 62-63 0.1842273714580429 1102 64-65 0.14348799558553793 1102 66-67 0.06446743446593217 1102 68-69 0.13451972644756438 1102 70-71 0.0013773186502774593 1102 72-73 0.18907855592510714 1102 74-75 0.0011067466753758026 1102 76 0.04466230833108398 1103 1 0.7131452307331116 1103 2 0.4246863404117036 1103 3 0.6475993053709104 1103 4 1.2512248983890473 1103 5 0.9717916753852087 1103 6 0.24597848530461164 1103 7 0.888458572260113 1103 8 0.3792687007025677 1103 9 0.5881570680557502 1103 10-11 0.36533546758604984 1103 12-13 0.8778418822893883 1103 14-15 0.5955319298402131 1103 16-17 0.6554931591203115 1103 18-19 0.808479236287404 1103 20-21 0.8784453981873526 1103 22-23 0.8591721531680321 1103 24-25 0.8489172435355243 1103 26-27 0.3911347274164285 1103 28-29 0.6835587574209541 1103 30-31 0.6384352769416637 1103 32-33 0.8110800688320055 1103 34-35 0.5148640762442227 1103 36-37 0.6164613088387583 1103 38-39 0.366228662769025 1103 40-41 0.6327932746640315 1103 42-43 0.5638330516123347 1103 44-45 0.712745378935665 1103 46-47 0.8302385869096369 1103 48-49 0.8604022706578007 1103 50-51 0.2597212546903265 1103 52-53 0.7822057181356499 1103 54-55 0.724713437622718 1103 56-57 0.8593314045375422 1103 58-59 0.708463025819043 1103 60-61 0.8189096723723921 1103 62-63 0.7872560939802185 1103 64-65 0.9971166164688796 1103 66-67 0.8420159517235177 1103 68-69 0.984739052345212 1103 70-71 0.9491890269850458 1103 72-73 0.9642781081028886 1103 74-75 0.7787393261236986 1103 76 0.9863116849335185 1104 1 0.027178649274937072 1104 2 0.2932971362895991 1104 3 0.38494890091714495 1104 4 0.32555115903533505 1104 5 -0.16930098025402884 1104 6 -0.007650517679600455 1104 7 -0.4102965431152441 1104 8 -0.45420218894287956 1104 9 -0.37829638632713625 1104 10-11 0.31506436702092344 1104 12-13 0.04970989700791506 1104 14-15 0.24006922479534154 1104 16-17 -0.09890864286737155 1104 18-19 0.24729151395245452 1104 20-21 -0.44758216656919103 1104 22-23 -0.1019634123985611 1104 24-25 0.02416398517038232 1104 26-27 -0.02419267571144701 1104 28-29 -0.08629329312334733 1104 30-31 0.01637602073920874 1104 32-33 0.2615769910095196 1104 34-35 0.34839228101971287 1104 36-37 0.07221020743300954 1104 38-39 0.3435796338783703 1104 40-41 -0.10339855525521813 1104 42-43 0.3490923643657098 1104 44-45 0.17446078256791964 1104 46-47 0.5048433990879602 1104 48-49 0.1116297696929891 1104 50-51 0.020422944722461978 1104 52-53 0.1852390258682206 1104 54-55 0.15517681134411987 1104 56-57 0.14478176772441032 1104 58-59 0.11773795121384723 1104 60-61 -0.18670816562448067 1104 62-63 0.10275104833826987 1104 64-65 0.4067191933571266 1104 66-67 0.17449528961913785 1104 68-69 0.03702669023586935 1104 70-71 0.10165587018230582 1104 72-73 -0.13195208474341769 1104 74-75 -0.03649771014914194 1104 76 0.03212748938958043 1105 1 -0.06580525971835272 1105 2 -0.03474385001263869 1105 3 0.12384943464248366 1105 4 0.2732761894461859 1105 5 0.4414777357145425 1105 6 0.467529470988147 1105 7 0.27043704195787654 1105 8 0.07935475166581085 1105 9 0.10532335715186036 1105 10-11 0.09739793941460562 1105 12-13 -0.6342579368279644 1105 14-15 -0.11981499603302126 1105 16-17 -0.1619997724077038 1105 18-19 0.10917076602375886 1105 20-21 0.11512164558484272 1105 22-23 0.16552431429602166 1105 24-25 0.06636275922665646 1105 26-27 -0.007912467927056355 1105 28-29 -0.09028038386458093 1105 30-31 -0.17937180889661164 1105 32-33 -0.17438785523241052 1105 34-35 0.21657163175294158 1105 36-37 0.0034935652667229533 1105 38-39 0.20221572195296034 1105 40-41 -0.16278788154090051 1105 42-43 -0.04502239252576601 1105 44-45 -0.021018599467687693 1105 46-47 0.2904122108259486 1105 48-49 0.08449575985849833 1105 50-51 0.33230358360794554 1105 52-53 -0.061085916578988986 1105 54-55 0.05839897501196134 1105 56-57 -0.24828693504960597 1105 58-59 0.08080808042530663 1105 60-61 0.02240459029226116 1105 62-63 0.13455465960236523 1105 64-65 -0.33523035038101057 1105 66-67 0.04481231533859642 1105 68-69 0.015335926022011392 1105 70-71 0.016013161060641323 1105 72-73 0.03709263803242635 1105 74-75 0.03538681181601078 1105 76 0.20010832758006813 1106 1 -0.13586068312603672 1106 2 -0.1859039640467408 1106 3 0.5355034997143235 1106 4 0.42778689145634274 1106 5 0.05366931143527509 1106 6 0.5114589650159189 1106 7 -0.4575790282726473 1106 8 0.05981785289764474 1106 9 0.13531141465684726 1106 10-11 -0.09110460549763388 1106 12-13 0.1690411036407582 1106 14-15 0.36086157483791226 1106 16-17 0.3673675508010703 1106 18-19 0.4428188932949553 1106 20-21 -0.005941216797076265 1106 22-23 0.06260833870042859 1106 24-25 0.24700135807883328 1106 26-27 0.3027114339831485 1106 28-29 0.45810639557701904 1106 30-31 0.0025958106119503555 1106 32-33 0.345248400372391 1106 34-35 0.14422903364052786 1106 36-37 0.30310361427621046 1106 38-39 0.26814340985051643 1106 40-41 0.09242933611353266 1106 42-43 0.11813924038909107 1106 44-45 0.0992749852286039 1106 46-47 0.18660694235590825 1106 48-49 0.022452825900302287 1106 50-51 0.10790469246958168 1106 52-53 0.03616868679048224 1106 54-55 -0.009168592555603539 1106 56-57 0.05940938323854539 1106 58-59 0.10965205871239903 1106 60-61 0.25588922146197746 1106 62-63 0.22591474648549337 1106 64-65 0.18661339771301044 1106 66-67 -0.20717039460436837 1106 68-69 -0.012651222515479787 1106 70-71 0.05265025677176993 1106 72-73 0.3360799852835896 1106 74-75 0.027571068577685764 1106 76 0.1422747788096288 1107 1 -0.32210191935439525 1107 2 -0.3895267999317049 1107 3 -0.5041664578380178 1107 4 -0.7877136570246037 1107 5 -0.4919347330155297 1107 6 -1.159591733701113 1107 7 -0.2569990692894848 1107 8 -0.21793862156222588 1107 9 0.09352956699146375 1107 10-11 0.01268611325087221 1107 12-13 0.21465754853034724 1107 14-15 -0.15818454525557257 1107 16-17 0.2398013753825552 1107 18-19 -0.027725295191721955 1107 20-21 0.13300951530575134 1107 22-23 -0.03868130693319216 1107 24-25 0.2009308663537439 1107 26-27 0.1675208964718209 1107 28-29 0.19734996881114597 1107 30-31 -0.13969063952397676 1107 32-33 0.14391106074345572 1107 34-35 0.3236280892994188 1107 36-37 0.18284511282153915 1107 38-39 -0.1741535329643895 1107 40-41 0.1250386745978176 1107 42-43 -0.15707419023999591 1107 44-45 -0.17533654717946234 1107 46-47 0.19957589356495475 1107 48-49 0.19940231795924745 1107 50-51 -0.2697163310435542 1107 52-53 0.11518523660686242 1107 54-55 -0.145030661521119 1107 56-57 0.07993497106703273 1107 58-59 -0.081826932082663 1107 60-61 0.003218834663677228 1107 62-63 -0.1153644064691477 1107 64-65 0.09183493632129824 1107 66-67 -0.03022471806504967 1107 68-69 -0.04915811429780348 1107 70-71 -0.04938341518893452 1107 72-73 0.06065765495497999 1107 74-75 0.11267527995747884 1107 76 0.03390829814954799 1108 1 -0.3369357229796286 1108 2 -0.2002915423871059 1108 3 -0.7699601352018348 1108 4 0.2824857786251158 1108 5 0.18066366958622027 1108 6 0.4136944110212539 1108 7 0.21867552060003703 1108 8 0.2073538362310714 1108 9 -0.20963749025661116 1108 10-11 0.34960514333361203 1108 12-13 -0.14936655592228476 1108 14-15 0.13937535717878546 1108 16-17 -0.07231972079613058 1108 18-19 0.3965561147274528 1108 20-21 -0.02152967834292241 1108 22-23 -0.013764763817789571 1108 24-25 -0.20528559545695657 1108 26-27 0.058624249095089453 1108 28-29 0.15997658892688804 1108 30-31 0.2607895364767927 1108 32-33 -0.06186731220599739 1108 34-35 0.32167103731223534 1108 36-37 -0.13907750423331322 1108 38-39 0.4216320139292904 1108 40-41 0.28016613167371673 1108 42-43 0.3227574612019666 1108 44-45 0.03352375221314219 1108 46-47 0.42529974453821495 1108 48-49 0.2009710204318651 1108 50-51 0.2490631419106748 1108 52-53 0.04270544321645531 1108 54-55 0.06434042270098672 1108 56-57 0.08592664929172145 1108 58-59 0.2501822505659561 1108 60-61 0.2242471000039572 1108 62-63 0.4359388600667913 1108 64-65 0.45667495736486785 1108 66-67 0.5954847850197496 1108 68-69 0.029606054242066193 1108 70-71 0.40975762455472164 1108 72-73 -0.014570237340208791 1108 74-75 0.3032984565799808 1108 76 0.09867657975077293 1109 1 0.4285600134309746 1109 2 0.32239771735831013 1109 3 0.18335263170042637 1109 4 0.053548927480040476 1109 5 0.31169544061415877 1109 6 0.37123653340651686 1109 7 0.4282555389494149 1109 8 0.24404085414420962 1109 9 0.3356539772827958 1109 10-11 0.4120859478892669 1109 12-13 0.13518573295717573 1109 14-15 0.26658662346191164 1109 16-17 0.27413530855700685 1109 18-19 0.3751344878927583 1109 20-21 0.20678802802400043 1109 22-23 0.13389882049251867 1109 24-25 -0.33084385277470574 1109 26-27 0.0665929260423681 1109 28-29 -0.271316924822429 1109 30-31 0.28412435001934 1109 32-33 0.42393246048082034 1109 34-35 0.06108346598342962 1109 36-37 -0.20068778927466013 1109 38-39 0.34664409361019466 1109 40-41 0.038843450630110965 1109 42-43 0.3871211309992475 1109 44-45 0.3378554181918716 1109 46-47 0.3455386385676249 1109 48-49 -0.026233594690914686 1109 50-51 0.15364050957703768 1109 52-53 0.26746771314010687 1109 54-55 0.4795479095600541 1109 56-57 -0.017695494377250043 1109 58-59 0.3279255118646134 1109 60-61 0.40582182089975305 1109 62-63 0.3055927454203484 1109 64-65 0.27688235619387314 1109 66-67 0.42611689956056154 1109 68-69 0.41368513262599294 1109 70-71 0.6370238607217757 1109 72-73 0.21092952889107153 1109 74-75 0.452953476543243 1109 76 -0.10319787372925049 1110 1 0.009149747629791705 1110 2 -0.3348173314615188 1110 3 0.10956377649232962 1110 4 0.11408668589785975 1110 5 0.026346844427791893 1110 6 0.12574642594834273 1110 7 0.002656103124309084 1110 8 0.26950470097540347 1110 9 0.3761319674486998 1110 10-11 -0.038101655848002736 1110 12-13 0.32585872970771135 1110 14-15 0.010510444266245145 1110 16-17 0.11081680689331819 1110 18-19 0.07082680997117308 1110 20-21 0.05775994395882833 1110 22-23 0.3085095865703309 1110 24-25 0.06578512430763794 1110 26-27 0.020589099939144262 1110 28-29 0.1956420244407866 1110 30-31 0.24696205622760203 1110 32-33 0.3901589174780682 1110 34-35 -0.11988400824296974 1110 36-37 0.3709990469042168 1110 38-39 0.2407502582339376 1110 40-41 0.3925963046225789 1110 42-43 0.6122485378987008 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2116 5 -1.0978983984977368 2116 6 -0.3917342691097545 2116 7 -0.16171254709339422 2116 8 0.09515681910641405 2116 9 -0.5730176528673994 2116 10-11 -0.18503872534579102 2116 12-13 -0.1738074932593534 2116 14-15 0.02522068971765634 2116 16-17 0.09487569598712753 2116 18-19 -0.24229413559009316 2116 20-21 -0.31518326783824335 2116 22-23 -0.05365453149652666 2116 24-25 -0.4354096571435804 2116 26-27 -0.17954781880769133 2116 28-29 -0.3737906147432284 2116 30-31 -0.241699547987011 2116 32-33 -0.21718598144662593 2116 34-35 0.18618351695569402 2116 36-37 -0.3432156448982937 2116 38-39 -0.036196473758138836 2116 40-41 -0.5017798029558591 2116 42-43 -0.4428106398671332 2116 44-45 0.0210269981886384 2116 46-47 -0.03812792506274576 2116 48-49 -0.3242851851687263 2116 50-51 -0.20243040941800672 2116 52-53 -0.22780660564327349 2116 54-55 -0.2965415969097407 2116 56-57 -0.28671234388217215 2116 58-59 -0.24959531204362406 2116 60-61 -0.3476484892940803 2116 62-63 -0.21709605153196065 2116 64-65 -0.6564279133859188 2116 66-67 -0.6235063356017108 2116 68-69 -0.2776041380203367 2116 70-71 0.07886942500925898 2116 72-73 -0.15074117393246667 2116 74-75 -0.19072995501574752 2116 76 -0.2704728639544527 2117 1 -0.15719770479500994 2117 2 -0.4969597501232741 2117 3 0.11958833143401293 2117 4 0.10883806711332866 2117 5 -0.3218708760551223 2117 6 0.46150660601791316 2117 7 0.14821165867986252 2117 8 0.26420697230750534 2117 9 -0.28246021589486503 2117 10-11 0.20673464454078783 2117 12-13 -0.2542568831427019 2117 14-15 0.08293933724762326 2117 16-17 -0.2568525198920284 2117 18-19 -0.16093091460422926 2117 20-21 -0.34926379248479833 2117 22-23 -0.01970301829973664 2117 24-25 0.24496662625157484 2117 26-27 -0.0012658212804126379 2117 28-29 -0.31646867742129103 2117 30-31 0.20968356226401852 2117 32-33 -0.08175943734083546 2117 34-35 0.2937941173964873 2117 36-37 -0.07319575563312242 2117 38-39 -0.12672058277281195 2117 40-41 -0.18816694481469654 2117 42-43 -0.007470103394524585 2117 44-45 0.14185374656255334 2117 46-47 0.07970360748576866 2117 48-49 0.13886591949182048 2117 50-51 0.12361700228978378 2117 52-53 -0.01477644921688892 2117 54-55 0.36046103707402466 2117 56-57 -0.04673169559963242 2117 58-59 0.03888826587390781 2117 60-61 -0.12638525218179097 2117 62-63 -0.15000042179482165 2117 64-65 -0.17761589381918697 2117 66-67 -0.1499182046550871 2117 68-69 -0.10269578990443762 2117 70-71 0.07248120805500236 2117 72-73 -1.294514758838126 2117 74-75 -0.49511526921237703 2117 76 -0.6494633493600332 2118 1 0.14528283834127365 2118 2 0.27801623927894425 2118 3 0.4397936634426216 2118 4 -0.2083005319924851 2118 5 -0.22109039363529348 2118 6 0.2168325429831981 2118 7 -0.019326013120100072 2118 8 0.3723507047135328 2118 9 0.165993722395136 2118 10-11 -0.5135413393417565 2118 12-13 -0.07070552254216977 2118 14-15 0.13957895749380356 2118 16-17 0.26471838036739825 2118 18-19 0.026660850036712702 2118 20-21 -0.018193526988305564 2118 22-23 0.010909113951747429 2118 24-25 0.41008447136941584 2118 26-27 0.44629061742357123 2118 28-29 0.4137824679118154 2118 30-31 0.048943925458814874 2118 32-33 0.13989646595441485 2118 34-35 -0.07510360297665386 2118 36-37 0.2857753764527615 2118 38-39 0.022684947944192402 2118 40-41 0.155490393596736 2118 42-43 0.010498655229966403 2118 44-45 0.2481980640544137 2118 46-47 -0.14717059906057273 2118 48-49 0.0686556197733168 2118 50-51 -0.012577731937739145 2118 52-53 0.04591240655393136 2118 54-55 -0.13510003815918026 2118 56-57 0.05209592486733072 2118 58-59 -0.13493963057693747 2118 60-61 -0.13630632079138394 2118 62-63 0.01669273932457571 2118 64-65 0.06749863949206514 2118 66-67 0.08131544329987861 2118 68-69 0.10531436630243718 2118 70-71 0.23124148845462855 2118 72-73 -0.04512578715407756 2118 74-75 0.0958059649875409 2118 76 0.012182618515446109 2119 1 -0.025501798362260075 2119 2 0.07537943116327384 2119 3 -0.5144467562852881 2119 4 -0.3037350323018764 2119 5 0.2502733734575493 2119 6 -0.18301581113608023 2119 7 -0.19909828284060183 2119 8 0.054382410903997425 2119 9 0.154596314319555 2119 10-11 -0.1372906965898153 2119 12-13 -0.0011379747606383717 2119 14-15 0.026087485395770216 2119 16-17 -0.1070961846093752 2119 18-19 -0.1893909535905891 2119 20-21 -0.164078607299615 2119 22-23 -0.17901101050246382 2119 24-25 -0.05296709694530932 2119 26-27 -0.05692858931160316 2119 28-29 -0.13042579243104058 2119 30-31 -0.49437101863266264 2119 32-33 -0.11833838470925429 2119 34-35 -0.16133258825654195 2119 36-37 -0.13343942035047007 2119 38-39 -0.5184457289716491 2119 40-41 -0.36665622356713357 2119 42-43 -0.5538931053438461 2119 44-45 -0.4291306588955308 2119 46-47 -0.6655108174654956 2119 48-49 -0.2690288173502893 2119 50-51 -0.2058859216868285 2119 52-53 -0.20191095214087085 2119 54-55 -0.363700756298293 2119 56-57 -0.323164444137646 2119 58-59 -0.18890900508125696 2119 60-61 -0.79765230871201 2119 62-63 -0.3050296615609014 2119 64-65 -0.636953125787997 2119 66-67 -0.8691872105030356 2119 68-69 -1.0439141134911907 2119 70-71 -0.8037078757656602 2119 72-73 -0.4482282091305265 2119 74-75 -0.5717137097581855 2119 76 0.16059513017213334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 29.0 13 352.0 14 1264.0 15 2153.0 16 2871.0 17 3324.0 18 3587.0 19 3350.0 20 2777.0 21 2127.0 22 1825.0 23 1589.0 24 1732.0 25 1900.0 26 2286.0 27 2538.0 28 3238.0 29 3984.0 30 4953.0 31 5974.0 32 7808.0 33 10136.0 34 13727.0 35 20645.0 36 38682.0 37 125522.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.34686052620793 18.06850912722219 10.63482541090199 26.949804935667892 2 22.548095374721004 19.38458786837722 29.753365651537226 28.31395110536455 3 20.151803646417484 20.33885674043216 28.432815521680645 31.07652409146971 4 16.02396664344029 18.38635034075708 31.029201894378346 34.56048112142429 5 16.496443382903646 30.853327272117536 32.77602441378231 19.874204931196505 6 30.723656999772704 31.57955532039363 17.716014651250312 19.98077302858335 7 29.98401478539197 27.27323538507972 19.43004698684294 23.31270284268537 8 26.5686190488611 29.895704858536444 18.64792657979752 24.887749512804938 9 25.004750850495395 16.76249101064563 19.577975429719086 38.65478270913989 10-11 27.47482049237442 22.49276193953937 24.08159539148871 25.950822176597498 12-13 26.496890521773803 20.589068199856168 23.646007608813107 29.26803366955692 14-15 25.438848170270482 21.27076866897937 21.596807428467095 31.69357573228305 16-17 26.176813613888132 23.493235161510288 22.72732353850797 27.602627686093605 18-19 25.31551236525284 23.003431790828436 23.591046789356604 28.09000905456212 20-21 24.952957264702487 23.46044497769895 23.9586322021962 27.62796555540237 22-23 26.28468586631293 22.970827914879663 23.28158942963711 27.462896789170298 24-25 25.599818163526137 23.580986164778125 22.927045567177025 27.89215010451871 26-27 24.933394939133223 23.704135661933204 23.94950311693054 27.412966282003033 28-29 25.627578035048238 23.16682378629743 24.71355166130721 26.492046517347124 30-31 25.40233182920786 22.948470971371933 24.289701273973165 27.359495925447046 32-33 24.77582464671306 23.732312364344335 24.321418923324856 27.17044406561775 34-35 25.248255226867084 23.363564889165453 24.624682810863984 26.76349707310348 36-37 25.385984033386432 22.61148217496204 24.423143205805363 27.579390585846163 38-39 24.398877681435913 22.987968238235588 25.717937348391978 26.89521673193652 40-41 24.589284316976745 22.426808956191568 25.972061272929842 27.011845453901845 42-43 23.669109783771095 22.088101262049463 26.049937959481767 28.192850994697675 44-45 22.93859665465602 22.012646577710875 27.257026601036614 27.79173016659649 46-47 23.084289403181394 22.404638320546404 27.36769347139988 27.143378804872327 48-49 22.511392725795815 22.810975768799395 27.359123309721916 27.318508195682874 50-51 22.361973820019152 23.7292872233794 26.42385783964855 27.4848811169529 52-53 21.87086629429935 23.59849910385918 26.520551620319484 28.01008298152199 54-55 21.759081576760703 23.45615989685997 26.242952905098498 28.54180562128083 56-57 21.729644934475527 23.601107413935082 26.283195403412414 28.38605224817698 58-59 22.196718745924514 23.202222280184667 25.998889605139112 28.6021693687517 60-61 22.32433963178114 22.59057356738569 26.33871514645661 28.746371654376556 62-63 22.150328088145976 22.934684189542168 25.89996013011741 29.015027592194446 64-65 22.93487049740473 22.93226218732883 24.97140174309636 29.161465572170076 66-67 22.666400867449408 23.33673655695618 24.06426876027022 29.932593815324193 68-69 22.604732964940585 23.696124423842935 24.05588490645482 29.643257704761655 70-71 23.382754599009587 23.475349606704103 24.447131417840097 28.694764376446212 72-73 23.350150723060814 23.365427967791096 23.86883181244015 29.415589496707938 74-75 23.073196905787487 24.069947684557256 23.95425007079725 28.902605338858002 76 23.492763775654687 24.106091544572458 23.269566124632973 29.131578555139882 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.0 14 2.0 15 4.0 16 7.5 17 9.0 18 16.0 19 21.5 20 38.0 21 56.0 22 61.0 23 96.0 24 152.5 25 179.0 26 314.0 27 552.0 28 797.0 29 939.0 30 1124.0 31 1608.0 32 2363.5 33 2820.0 34 3218.5 35 4023.5 36 5050.5 37 5671.0 38 6564.0 39 8384.5 40 10203.0 41 11717.0 42 12340.0 43 12679.5 44 13681.5 45 14479.5 46 14615.0 47 14689.5 48 14345.5 49 13378.0 50 12829.0 51 11923.5 52 9914.5 53 8118.5 54 7426.0 55 6992.5 56 6083.0 57 5390.5 58 5174.0 59 4930.0 60 4732.5 61 4792.5 62 4806.0 63 4888.0 64 5078.5 65 5275.0 66 5484.0 67 5605.0 68 5611.0 69 5806.5 70 5817.0 71 5638.0 72 5639.0 73 5345.5 74 4692.5 75 4334.0 76 3909.5 77 3014.0 78 2202.5 79 1862.0 80 1491.5 81 852.5 82 409.0 83 234.0 84 170.5 85 68.5 86 24.5 87 19.0 88 18.0 89 15.0 90 10.5 91 9.0 92 10.0 93 6.0 94 4.5 95 7.5 96 8.0 97 7.0 98 5.5 99 31.0 100 57.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 1.8630786256441595E-4 34-35 0.0 36-37 1.8630786256441595E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.726157251288319E-4 76 3.726157251288319E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 268373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.879813913408455 #Duplication Level Percentage of deduplicated Percentage of total 1 71.65370965898903 37.89034832972738 2 14.643251835393233 15.486648642855421 3 5.442360084864804 8.63372965612238 4 2.368595354429163 5.010035263135116 5 1.2678541031793822 3.352194452273854 6 0.8241254290231089 2.6147759596829943 7 0.6214949078391386 2.300517455226521 8 0.47070349573287584 1.9912570610196259 9 0.396827761563568 1.8885760368440307 >10 2.2864992605693613 20.072529297464538 >50 0.024578108416305287 0.7593878456481442 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008570161677963133 0.0 2 0.0 0.0 0.0 0.010805856028736125 0.0 3 0.0 0.0 0.0 0.01192370320412262 0.0 4 0.0 0.0 0.0 0.036888956787754355 0.0 5 0.0 0.0 0.0 0.043968655565202165 0.0 6 0.0 0.0 0.0 0.04471388701545983 0.0 7 0.0 0.0 0.0 0.04471388701545983 0.0 8 0.0 0.0 0.0 0.04694958136623282 0.0 9 0.0 0.0 0.0 0.04955789144213464 0.0 10 0.0 0.0 0.0 0.05216620151803646 0.0 11 0.0 0.0 0.0 0.05291143296829413 0.0 12 0.0 0.0 0.0 0.05291143296829413 0.0 13 0.0 0.0 0.0 0.05328404869342296 0.0 14 0.0 0.0 0.0 0.05551974304419595 0.0 15 0.0 0.0 0.0 0.056637590219582444 0.0 16 0.0 0.0 0.0 0.05850066884522661 0.0 17 0.0 0.0 0.0 0.0607363631959996 0.0 18 0.0 0.0 0.0 0.06818867769857624 0.0 19 0.0 0.0 0.0 0.0700517563242204 0.0 20 0.0 0.0 0.0 0.07452314502576637 0.0 21 0.0 0.0 0.0 0.0760136079262817 0.0 22 0.0 0.0 0.0 0.08048499662782768 0.0 23 0.0 0.0 0.0 0.08570161677963134 0.0 24 0.0 0.0 0.0 0.08793731113040433 0.0 25 0.0 0.0 0.0 0.09576224135810979 0.0 26 0.0 0.0 0.0 0.10917640746274775 0.0 27 0.0 0.0 0.0 0.18966140409057544 0.0 28 0.0 0.0 0.0 0.26381193339121295 0.0 29 0.0 0.0 0.0 0.33870769414210816 0.0 30 0.0 0.0 0.0 0.4560816475576902 0.0 31 0.0 0.0 0.0 0.5667485179209533 0.0 32 0.0 0.0 0.0 0.7128138821714554 0.0 33 0.0 0.0 0.0 0.8614875564978594 0.0 34 0.0 0.0 0.0 1.075368982721809 0.0 35 0.0 0.0 0.0 1.3704806370238436 0.0 36 0.0 0.0 0.0 1.7811031661158163 0.0 37 0.0 0.0 0.0 2.4074702000573827 0.0 38 0.0 0.0 0.0 3.0681178807108016 0.0 39 0.0 0.0 0.0 3.7384535702175703 3.726157251288319E-4 40 0.0 0.0 0.0 4.53287029619224 3.726157251288319E-4 41 0.0 0.0 0.0 5.4599382203127735 3.726157251288319E-4 42 0.0 0.0 0.0 6.487612390218092 3.726157251288319E-4 43 0.0 0.0 0.0 7.513050865772637 3.726157251288319E-4 44 0.0 0.0 0.0 8.436020016916753 3.726157251288319E-4 45 0.0 0.0 0.0 9.32396328989876 3.726157251288319E-4 46 0.0 0.0 0.0 10.150797583959639 3.726157251288319E-4 47 0.0 0.0 0.0 10.905717043070652 3.726157251288319E-4 48 0.0 0.0 0.0 11.59840967608515 3.726157251288319E-4 49 0.0 0.0 0.0 12.274334601468851 3.726157251288319E-4 50 0.0 0.0 0.0 12.927529967619693 3.726157251288319E-4 51 0.0 0.0 0.0 13.644070007042437 3.726157251288319E-4 52 0.0 0.0 0.0 14.466060296676641 3.726157251288319E-4 53 0.0 0.0 0.0 15.35325833820839 3.726157251288319E-4 54 0.0 0.0 0.0 16.203567422952382 3.726157251288319E-4 55 0.0 0.0 0.0 16.958859497788527 3.726157251288319E-4 56 0.0 0.0 0.0 17.642609353399934 3.726157251288319E-4 57 0.0 0.0 0.0 18.31108196428106 3.726157251288319E-4 58 0.0 0.0 0.0 18.91285636036412 3.726157251288319E-4 59 0.0 0.0 0.0 19.49376427583997 3.726157251288319E-4 60 0.0 0.0 0.0 20.100755292074837 3.726157251288319E-4 61 0.0 0.0011178471753864956 0.0 20.662659805569113 3.726157251288319E-4 62 0.0 0.0011178471753864956 0.0 21.252883114173184 3.726157251288319E-4 63 0.0 0.0011178471753864956 0.0 21.902724938797867 3.726157251288319E-4 64 0.0 0.0011178471753864956 0.0 22.559646462199996 3.726157251288319E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAACG 30 8.362673E-6 58.333332 34 TTAGACC 20 0.0069462606 52.5 62 CTGTGTA 20 0.0069462606 52.5 31 GGTACCC 35 2.0831601E-5 50.0 46 TAACGGC 35 2.0831601E-5 50.0 36 TAACCGT 35 2.0831601E-5 50.0 53 TTAACGG 35 2.0831601E-5 50.0 35 ATTAGAG 45 9.183056E-5 38.888885 3 GAACCTC 45 9.183056E-5 38.888885 17 GTGCAAA 50 1.7069984E-4 35.0 58 GACACAT 40 0.0024161413 35.0 26 CGCAATA 50 1.7069984E-4 35.0 57 CAAAGGT 50 1.7069984E-4 35.0 61 CAGTGAC 65 2.0980518E-5 32.307693 22 CGCGGTA 55 2.9873874E-4 31.81818 43 CGGTACC 55 2.9873874E-4 31.81818 45 CGATAGA 55 2.9873874E-4 31.81818 37 GATACTA 45 0.0043026353 31.111109 1 TGAGACC 45 0.0043026353 31.111109 19 CTCCGGT 60 4.974238E-4 29.166666 7 >>END_MODULE