FastQCFastQC Report
Wed 25 May 2016
SRR1780168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268373
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT102683.826018265622846No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC30161.123809026988557No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG26600.9911578288426929No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG22940.8547804734455403RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC6850.25524177171324985No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3390.126316730818674RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3340.12445365219302985No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA207.917565E-570.03
GATATTA150.002223041470.01
GCATATC252.3877245E-455.99999635
GATCTTA200.006946260652.520
TCTTTAT1150.048.6956561
CTTTATA1150.048.6956562
ATCGTAT305.871306E-446.66666439
ATATCGT305.871306E-446.66666437
TATGTCA404.5853507E-543.7552
CAACCGT350.001254049740.09
CAGCTTA350.001254049740.01
ATCGGTT350.001254049740.025
CAATACG350.001254049740.069
ACCGTAT459.183056E-538.88888511
AGCGTCA601.2130809E-535.03
TTCCCTA400.002416141335.024
CTTATAT501.7069984E-435.01
AGCATGA400.002416141335.045
TAAGCGC400.002416141335.047
TATCGGC400.002416141335.015