FastQCFastQC Report
Wed 25 May 2016
SRR1780167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510705
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTT401.8176317E-533.0010323
TTAAGCC454.0886567E-529.30838231
CATCGTT407.0615613E-427.47660832
CGGGAAT508.274957E-526.42673913
CGAGAAT450.001386508824.47640814
TTGCATC551.572559E-424.04318819
AATTGCA602.834937E-422.03958917
ATACTGC500.002591184321.98128931
TATACCG602.8996042E-421.9662256
ACGAGAG654.9062376E-420.3202824
TAGGACG654.978109E-420.2765125
TCGTTGT550.00444888620.02418734
TCCGTAT550.004453944520.02025613
TCACTGA550.004497134619.98690427
GATAGCA550.004520138719.9692942
TGCATCT708.001136E-418.89664320
GAATTGC708.017012E-418.89107516
ATAGCAT708.1988086E-418.8281923
CGTTTGG600.007434410518.32133129
TTTTGAC600.007442819418.3177431
Produced by FastQC (version 0.11.5)