FastQCFastQC Report
Wed 25 May 2016
SRR1780167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510705
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT123402.4162677083639283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC33860.6630050616304912No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG30400.5952555780734474No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG29100.5698005698005698TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC7470.1462683936910741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG3700.035.0787444
GTCTTCT3800.033.57670643
CCGTCTT4000.031.8978741
ATGCCGT16750.031.38894844
CGTCTTC4100.031.11987342
ATTTCGC300.005743819829.33137728
TTGCTGC407.029276E-427.49816522
GCCGTCT4750.026.86136440
TATGCCG19800.026.55378343
GGCTTAT508.220038E-526.4526231
ACTTTGC609.743635E-625.66495538
CGCAAAG609.743635E-625.66495512
CTTTATA1102.0008883E-1024.0054472
GTAAAGG500.002575019822.0049933
TAAACGG500.002579416421.99853126
AAGTTGT500.002579416421.99853119
GATGCGT500.002579416421.9985319
GTAAACG500.002579416421.99853125
TGCCGTC5850.021.8105139
GTATGCC24250.021.68102642
Produced by FastQC (version 0.11.5)