FastQCFastQC Report
Wed 25 May 2016
SRR1780166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217021
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTG200.00694447752.5000045
CATTAAA751.4844609E-632.66666861
GCTCTAC604.9711374E-429.1666661
GTATCAT500.00719768827.99999857
GACTCTT500.00719768827.99999811
TAGGCTT657.938435E-426.9230774
TAATATA700.001223350225.07
CGTATCA700.001223350225.056
TATCATT750.001828265723.33333458
CCGTATC1052.0307612E-523.33333455
TCATTAA800.002660247721.87500260
TAAATCT800.002660247721.87500239
ATCATTA1003.919465E-421.059
AAAACAA1355.98764E-620.7407468
ACTTCAA850.003781008820.58823620
ATCCGCG1205.69036E-520.41666635
AAGTGTA2203.4560799E-919.09090870
GAAGTGT2508.5310603E-1018.269
CACGCGA1000.00966581317.52
CATCTTA1000.00966581317.59
Produced by FastQC (version 0.11.5)