##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217021 Sequences flagged as poor quality 0 Sequence length 76 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.522124587021533 34.0 32.0 34.0 12.0 34.0 2 30.61108372000866 34.0 32.0 34.0 21.0 34.0 3 30.3886674561448 34.0 32.0 34.0 11.0 34.0 4 30.22752636841596 34.0 31.0 34.0 11.0 34.0 5 30.235438045166138 34.0 31.0 34.0 11.0 34.0 6 33.31674814879666 37.0 34.0 38.0 21.0 38.0 7 33.661622607950385 38.0 34.0 38.0 21.0 38.0 8 33.73266642398662 38.0 34.0 38.0 21.0 38.0 9 33.97811732505149 38.0 34.0 38.0 21.0 38.0 10-11 33.65759765184015 38.0 34.0 38.0 21.0 38.0 12-13 33.751957183867 38.0 34.0 38.0 21.0 38.0 14-15 33.68818685749305 38.0 34.0 38.0 21.0 38.0 16-17 33.73070117638385 38.0 34.0 38.0 21.0 38.0 18-19 33.73318480700024 38.0 34.0 38.0 21.0 38.0 20-21 33.76838416558766 38.0 34.0 38.0 21.0 38.0 22-23 33.793748070463224 38.0 34.0 38.0 21.0 38.0 24-25 33.75484400127176 38.0 34.0 38.0 21.0 38.0 26-27 33.72894097806203 38.0 34.0 38.0 21.0 38.0 28-29 33.82368756940572 38.0 34.0 38.0 21.0 38.0 30-31 33.83447224001364 38.0 34.0 38.0 21.0 38.0 32-33 33.91718312974321 38.0 34.0 38.0 21.0 38.0 34-35 33.83434552416587 38.0 34.0 38.0 21.0 38.0 36-37 33.74107805235438 38.0 34.0 38.0 21.0 38.0 38-39 33.724708668746345 38.0 34.0 38.0 21.0 38.0 40-41 33.805859801586024 38.0 34.0 38.0 21.0 38.0 42-43 33.84257514249773 38.0 34.0 38.0 21.0 38.0 44-45 33.92987544984126 38.0 34.0 38.0 21.0 38.0 46-47 33.89638560323655 38.0 34.0 38.0 21.0 38.0 48-49 33.87329797577193 38.0 34.0 38.0 21.0 38.0 50-51 33.78280673298897 38.0 34.0 38.0 21.0 38.0 52-53 33.820259329742285 38.0 34.0 38.0 21.0 38.0 54-55 33.79965072504504 38.0 34.0 38.0 21.0 38.0 56-57 33.75265066514301 38.0 34.0 38.0 21.0 38.0 58-59 33.81001377746854 38.0 34.0 38.0 21.0 38.0 60-61 33.81349270347109 38.0 34.0 38.0 21.0 38.0 62-63 33.67134516936149 38.0 34.0 38.0 20.5 38.0 64-65 33.65532137442921 38.0 34.0 38.0 20.0 38.0 66-67 33.53409347482501 38.0 34.0 38.0 20.0 38.0 68-69 33.45796950525525 38.0 34.0 38.0 20.0 38.0 70-71 33.40531331069343 38.0 34.0 38.0 19.0 38.0 72-73 33.437588989084006 38.0 34.0 38.0 19.0 38.0 74-75 33.48137737822607 38.0 34.0 38.0 19.5 38.0 76 31.325604434593888 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1498172887558198 1101 2 0.05774870787485398 1101 3 0.2270905216775212 1101 4 0.4611765346834069 1101 5 -0.3991984215242148 1101 6 0.14302429496968472 1101 7 -0.35904777125998066 1101 8 -0.6256136685981915 1101 9 -0.41542360408890033 1101 10-11 -0.6761180118519334 1101 12-13 -0.16464838783438296 1101 14-15 -0.23631498390525252 1101 16-17 -0.2696471036764905 1101 18-19 -0.16672002835041866 1101 20-21 -0.3007541340587636 1101 22-23 -0.19345838224384693 1101 24-25 -0.4836000434589067 1101 26-27 -0.8349157937137335 1101 28-29 -0.5298346606750002 1101 30-31 -0.6368307374868749 1101 32-33 -0.34993501200374055 1101 34-35 -0.4918083414760943 1101 36-37 -0.06661249896986021 1101 38-39 -0.38998748748787904 1101 40-41 -0.2621201744558661 1101 42-43 -0.4502255137919917 1101 44-45 -0.137130371149226 1101 46-47 -0.19788330391531161 1101 48-49 -0.3889737890639964 1101 50-51 -0.35386884814193564 1101 52-53 -0.32498470122261125 1101 54-55 -0.22143270123310543 1101 56-57 -0.17095989713556747 1101 58-59 -0.01152714894467266 1101 60-61 -0.16817540588301227 1101 62-63 -0.2706792758819816 1101 64-65 -0.21425815540106186 1101 66-67 -0.4237342362181096 1101 68-69 -0.16422857996850837 1101 70-71 -0.15181984729899511 1101 72-73 -0.3530938464478268 1101 74-75 -0.6894447398790717 1101 76 -0.9843151203653449 1102 1 0.2857653488130545 1102 2 0.4943840150137575 1102 3 0.5141083990144466 1102 4 0.4031714337474348 1102 5 0.15164251108087257 1102 6 0.12646429881944954 1102 7 -0.20093078658684504 1102 8 0.026842961415525224 1102 9 0.3180885828170972 1102 10-11 0.06162891435291584 1102 12-13 0.21483598453394137 1102 14-15 0.5846680290155106 1102 16-17 0.32829327311906553 1102 18-19 0.30037051326311115 1102 20-21 0.47280461091850157 1102 22-23 -0.18508514772109663 1102 24-25 0.05538482216029905 1102 26-27 -0.30244244129199416 1102 28-29 -0.21515563466503806 1102 30-31 0.03229115563407703 1102 32-33 0.22993553971542013 1102 34-35 0.08863065181088103 1102 36-37 -0.04507187193971163 1102 38-39 0.21009131234850287 1102 40-41 0.06404498037679218 1102 42-43 0.07026244366812762 1102 44-45 0.07570137014670308 1102 46-47 -0.34112155612290707 1102 48-49 0.041980110024091744 1102 50-51 0.010417983262506425 1102 52-53 0.2864899986161831 1102 54-55 -0.3333344841545909 1102 56-57 0.057535567362400286 1102 58-59 0.05185859730782738 1102 60-61 0.14362832838710204 1102 62-63 -0.08877496655352246 1102 64-65 -0.08102327173608614 1102 66-67 0.020176521463369568 1102 68-69 -0.08618666567385702 1102 70-71 0.03859905503733074 1102 72-73 0.21954937676624553 1102 74-75 -0.11149906965938783 1102 76 -0.05134911858483093 1103 1 0.16051979931082982 1103 2 -0.001840226634776343 1103 3 0.39140599109898844 1103 4 0.2884518192810219 1103 5 0.08654476429319047 1103 6 -0.5927188908477206 1103 7 0.1644023538312993 1103 8 0.06841247487970037 1103 9 -0.2905597680362746 1103 10-11 -0.2095634684650136 1103 12-13 -0.0743851574868799 1103 14-15 -0.26160407043969514 1103 16-17 -0.26090867013047614 1103 18-19 -0.2286641502575506 1103 20-21 -0.16457372730263842 1103 22-23 0.11990124619316589 1103 24-25 0.08993148073936652 1103 26-27 -0.3378530445785941 1103 28-29 -0.276367717091766 1103 30-31 -0.3978809655240809 1103 32-33 -0.0685065773803899 1103 34-35 -0.40569736337005935 1103 36-37 -0.2830114264169197 1103 38-39 -0.8709469794616851 1103 40-41 -0.4110147027600064 1103 42-43 -0.55709775310536 1103 44-45 -0.002343026316275143 1103 46-47 0.1846520987110054 1103 48-49 0.006932613933408049 1103 50-51 -0.5532802009232682 1103 52-53 -0.05829489167258117 1103 54-55 -0.046041566553498114 1103 56-57 -0.01780987139908774 1103 58-59 -0.3674731741544548 1103 60-61 -0.043100590527309635 1103 62-63 -0.2886633910856631 1103 64-65 -0.17674723235912637 1103 66-67 0.027463707257396663 1103 68-69 0.030213536887089276 1103 70-71 0.11744061787296545 1103 72-73 0.059089385336129396 1103 74-75 0.02307875214262367 1103 76 -0.36359904763284945 1104 1 0.5469159867629827 1104 2 0.15600083622310734 1104 3 0.15626874107144673 1104 4 0.5098598291344345 1104 5 -0.6202229601276272 1104 6 -0.035356938922660675 1104 7 0.010112248771534382 1104 8 -0.13436218760980978 1104 9 -0.15153284912071996 1104 10-11 0.23134006243566319 1104 12-13 0.22496453982009967 1104 14-15 -0.23735472935846502 1104 16-17 -0.1796335457622149 1104 18-19 -0.0479325456031674 1104 20-21 0.03255527953091075 1104 22-23 -0.08303161330246667 1104 24-25 0.09224793690043498 1104 26-27 0.21681955824239196 1104 28-29 0.06984501833221657 1104 30-31 -0.08468620523491666 1104 32-33 0.31667770800700623 1104 34-35 0.3378215030353928 1104 36-37 -0.042516665280274424 1104 38-39 0.33846489491859444 1104 40-41 -0.22622223745741366 1104 42-43 -0.01101016281300815 1104 44-45 0.051446780711629 1104 46-47 0.006977086210618211 1104 48-49 -0.28876583997335104 1104 50-51 0.045813273732726145 1104 52-53 -0.08168121158647068 1104 54-55 0.06906088013270306 1104 56-57 0.0114368001076528 1104 58-59 -0.1551784418322626 1104 60-61 -0.025009632898679968 1104 62-63 -0.08640004391950384 1104 64-65 0.20011476052766142 1104 66-67 0.02078977106164359 1104 68-69 0.08466296893158898 1104 70-71 0.09012479732486156 1104 72-73 0.46201009756165234 1104 74-75 0.2566778187802896 1104 76 0.424010171862669 1105 1 0.4609001978609477 1105 2 0.30634982697957014 1105 3 0.14989773045595456 1105 4 0.3738706218379271 1105 5 0.24002353424450362 1105 6 0.3295599149585442 1105 7 0.19286221590181185 1105 8 -0.14371433957656166 1105 9 0.3518655948549778 1105 10-11 0.535785192857027 1105 12-13 -0.6727691639407709 1105 14-15 -0.09241495241530373 1105 16-17 0.10211003606626434 1105 18-19 0.42315352735047895 1105 20-21 0.06918930512927801 1105 22-23 0.136077988659423 1105 24-25 -0.02629316169159779 1105 26-27 0.22905424237860217 1105 28-29 -0.010804430313832825 1105 30-31 0.4016142640876197 1105 32-33 -0.052076329252969344 1105 34-35 0.44966575197641845 1105 36-37 0.07137408146276414 1105 38-39 0.22710368805653758 1105 40-41 0.21025344180264227 1105 42-43 0.3284969627722205 1105 44-45 0.2775076154312117 1105 46-47 0.31294136967914454 1105 48-49 0.2817411541329662 1105 50-51 0.10224202073872135 1105 52-53 0.18680586632335405 1105 54-55 -0.08706746614844718 1105 56-57 -0.26814881266980706 1105 58-59 -0.3675726156016452 1105 60-61 0.33661167354436117 1105 62-63 0.3492338844988012 1105 64-65 -0.30907018456560564 1105 66-67 0.01792153007794184 1105 68-69 -0.10404642266404807 1105 70-71 -0.03633647641994031 1105 72-73 0.13719574006478297 1105 74-75 -0.08205186629914607 1105 76 0.2599016274485244 1106 1 0.04830715483312176 1106 2 0.47097264377629955 1106 3 0.39036257961645404 1106 4 0.602168089265831 1106 5 0.4961241164320427 1106 6 0.6415144660435246 1106 7 0.25893543414893827 1106 8 0.4148028276362581 1106 9 0.8699280477000357 1106 10-11 0.4228329277308447 1106 12-13 0.40797979724296596 1106 14-15 0.6657717079452752 1106 16-17 0.6853166844903029 1106 18-19 0.4475277038985652 1106 20-21 0.5856808650990928 1106 22-23 0.3893295679979616 1106 24-25 0.5152510428960824 1106 26-27 0.48518297676820765 1106 28-29 0.4253125927597097 1106 30-31 0.19115403188826008 1106 32-33 0.6722432320231135 1106 34-35 0.7299349996369742 1106 36-37 0.8545936798997502 1106 38-39 0.6356765966294446 1106 40-41 0.2856168867313045 1106 42-43 0.33531261089221687 1106 44-45 0.4477749487420155 1106 46-47 0.40720620307441635 1106 48-49 0.6448229193886448 1106 50-51 0.900050090285923 1106 52-53 0.7522090621613344 1106 54-55 0.39492638541062774 1106 56-57 0.5257629921115594 1106 58-59 0.6070425437961191 1106 60-61 0.6845982280526499 1106 62-63 0.25654275919897884 1106 64-65 0.8127894372940219 1106 66-67 0.6113136452091936 1106 68-69 0.6119738694431973 1106 70-71 0.5912746403216076 1106 72-73 0.9110209486726006 1106 74-75 0.385156448735259 1106 76 0.6693866673683466 1107 1 0.11444716175273584 1107 2 -0.21636500608009257 1107 3 -0.11817710658440106 1107 4 -0.6282524147678679 1107 5 -0.4659607751880799 1107 6 -0.92034471425643 1107 7 -0.06835421772841954 1107 8 -0.17905958181835757 1107 9 0.030855825569346962 1107 10-11 -0.3304635009778778 1107 12-13 0.4137736036364785 1107 14-15 0.1614579307679307 1107 16-17 0.2635893613386884 1107 18-19 0.06175630388428743 1107 20-21 0.34953794754688516 1107 22-23 0.10268992078860606 1107 24-25 0.4992205965807841 1107 26-27 0.31545602705624987 1107 28-29 0.4005131792796348 1107 30-31 0.13198032203987964 1107 32-33 0.29342714556084815 1107 34-35 0.28895768609594086 1107 36-37 0.20724427767778764 1107 38-39 -0.479423820195116 1107 40-41 0.11378796602681973 1107 42-43 0.12391418922392461 1107 44-45 -0.013215709774875961 1107 46-47 -0.08338229388589724 1107 48-49 0.10700528974192736 1107 50-51 -0.1831281008382959 1107 52-53 -0.033363810742876865 1107 54-55 0.037105295955349504 1107 56-57 0.24740491840116619 1107 58-59 -0.17841088333646837 1107 60-61 -0.11661517623421247 1107 62-63 0.09843774577162634 1107 64-65 -0.1107611500496759 1107 66-67 -0.15706727422120537 1107 68-69 0.1973501373531974 1107 70-71 0.2018292531250978 1107 72-73 0.010846036128867809 1107 74-75 0.2692858126247444 1107 76 -0.004433016196465189 1108 1 -1.0441503071895575 1108 2 -0.9108218901921461 1108 3 -0.6918531112948898 1108 4 -0.0924593444920383 1108 5 0.049787844008815085 1108 6 -0.03912695372832786 1108 7 -0.6657236426840498 1108 8 -0.6336133294755513 1108 9 -0.8855081425187592 1108 10-11 -0.33626867919684855 1108 12-13 -0.40425064542225897 1108 14-15 -0.4013375113378572 1108 16-17 -0.6605162265599986 1108 18-19 -0.41775556355861454 1108 20-21 -0.6774436915037185 1108 22-23 -0.2873226847412411 1108 24-25 -0.2973369327353623 1108 26-27 -0.5217875084631487 1108 28-29 -0.3028919724013761 1108 30-31 -0.11858775611439398 1108 32-33 -0.42665545383209746 1108 34-35 -0.2531962008855331 1108 36-37 -0.4521949421062601 1108 38-39 -0.26952930857645896 1108 40-41 -0.35287787132866555 1108 42-43 -0.21864108436582796 1108 44-45 -0.36053952261593025 1108 46-47 -0.5422774855397066 1108 48-49 -0.4615248391330269 1108 50-51 -0.12503070653401238 1108 52-53 -0.23659480707731717 1108 54-55 0.03751165843068094 1108 56-57 0.24616095163995766 1108 58-59 -0.06201706004608809 1108 60-61 -0.22399438706170116 1108 62-63 -0.47063143536652063 1108 64-65 -0.16008028557570952 1108 66-67 -0.2994185372621203 1108 68-69 -0.14529221390984048 1108 70-71 0.0015423114588486442 1108 72-73 -0.3956325427635008 1108 74-75 -0.1477084319557136 1108 76 -0.6306707631238666 1109 1 -0.27312959781268376 1109 2 -0.13219659615589308 1109 3 -0.07868951182991779 1109 4 -0.00935662782767821 1109 5 -0.3753366889924301 1109 6 0.31608037065707606 1109 7 -0.21515056026165524 1109 8 -0.2823841426299296 1109 9 0.30733928149305 1109 10-11 0.1074695016988656 1109 12-13 -0.28658713820394865 1109 14-15 -0.41474210522189736 1109 16-17 -0.18880424371788251 1109 18-19 -0.029653654052594902 1109 20-21 -0.3403224793825075 1109 22-23 -0.2853451468048007 1109 24-25 -0.7863249867919109 1109 26-27 -0.45658352801944346 1109 28-29 -0.43630948081888477 1109 30-31 -0.168354833278741 1109 32-33 -0.3330827403963639 1109 34-35 -0.20059542140119646 1109 36-37 -0.5847274590565874 1109 38-39 -0.14843826111555103 1109 40-41 -0.272055376978777 1109 42-43 -0.35547460219250127 1109 44-45 -0.255539753232604 1109 46-47 -0.253712613070725 1109 48-49 -0.5830828853486096 1109 50-51 -0.15712966414666596 1109 52-53 -0.2991149860358533 1109 54-55 -0.18052775395804588 1109 56-57 0.02012490625460117 1109 58-59 -0.26534601166271443 1109 60-61 -0.315768290479447 1109 62-63 -0.05689071768744469 1109 64-65 -0.21605671655213854 1109 66-67 -0.07646410711591045 1109 68-69 -0.26791686221942257 1109 70-71 0.1365597230173492 1109 72-73 -0.201620504573377 1109 74-75 -0.13594986454645408 1109 76 -0.1916054524832127 1110 1 0.445195282502052 1110 2 0.6216131872256305 1110 3 0.7464515034803156 1110 4 0.792404286046331 1110 5 0.8687560730116459 1110 6 0.6475611499147931 1110 7 0.38482218467838436 1110 8 0.6539101172174426 1110 9 1.0712334370601866 1110 10-11 0.876065155109309 1110 12-13 1.0882143738593797 1110 14-15 0.7231862732205272 1110 16-17 0.7396008612733453 1110 18-19 0.905328215407522 1110 20-21 0.865127347607114 1110 22-23 0.8933828713968381 1110 24-25 0.7749633480424194 1110 26-27 0.9035180267039777 1110 28-29 0.8193133565513548 1110 30-31 0.6583977855770229 1110 32-33 0.7332655555698864 1110 34-35 0.502599938128597 1110 36-37 0.7409912450245599 1110 38-39 0.8225943501561872 1110 40-41 0.8992337873573817 1110 42-43 1.083163000310229 1110 44-45 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-0.2832346978360718 2116 7 -0.1206330975935046 2116 8 0.006854410992190196 2116 9 -0.8847346417452613 2116 10-11 -0.016794659722826566 2116 12-13 -0.012285953457563892 2116 14-15 0.3679772579769107 2116 16-17 -0.2157032317470069 2116 18-19 -0.05678095258400617 2116 20-21 -0.2207278847879124 2116 22-23 0.09313504571647968 2116 24-25 -0.3688129542113856 2116 26-27 0.04957931290367412 2116 28-29 -0.04734551685492505 2116 30-31 -0.04101927854591736 2116 32-33 -0.23364426082090972 2116 34-35 -0.05075739844672711 2116 36-37 -0.5122959522072676 2116 38-39 -0.3840392730864224 2116 40-41 -0.9152538837046862 2116 42-43 -0.36495103067577617 2116 44-45 -0.5636972257736375 2116 46-47 -0.4750966683588942 2116 48-49 -0.6161385437467359 2116 50-51 -0.15585698736028775 2116 52-53 -0.16829923878174924 2116 54-55 0.02641874733777172 2116 56-57 -0.33846913299012016 2116 58-59 -0.22274142077046122 2116 60-61 -0.7292632923306073 2116 62-63 -0.23759385232893493 2116 64-65 -0.6460116715070896 2116 66-67 -0.2821194199630028 2116 68-69 -0.21058932920695383 2116 70-71 -0.44969073977420493 2116 72-73 -0.363773410904372 2116 74-75 -0.524048408295684 2116 76 -0.4018146342677369 2117 1 0.04861063557138223 2117 2 -0.4751316545019115 2117 3 -0.48208880153057976 2117 4 -0.21788021991291373 2117 5 -0.4081246139036452 2117 6 -0.022123586724958955 2117 7 0.1596972327368249 2117 8 -0.22502747088969244 2117 9 -0.19964955666017659 2117 10-11 -0.007985850914018044 2117 12-13 -0.38228094845255356 2117 14-15 -0.1175833025836468 2117 16-17 -0.25386639491016894 2117 18-19 -0.0977218935249482 2117 20-21 -0.8143965534565822 2117 22-23 -0.14262571504427513 2117 24-25 -0.10154568694412092 2117 26-27 -0.16303329970894254 2117 28-29 -0.77056874007814 2117 30-31 -0.2334867460133907 2117 32-33 -0.18204265921930585 2117 34-35 -0.10193357462291175 2117 36-37 -0.21322187813319715 2117 38-39 -0.12828351733067223 2117 40-41 -0.2460596895104885 2117 42-43 -0.3794154951402362 2117 44-45 -0.150859387935796 2117 46-47 -0.1148364080986326 2117 48-49 -0.13475716236535362 2117 50-51 0.026400269896967643 2117 52-53 -0.10393487441738358 2117 54-55 -0.13454221362319174 2117 56-57 -0.1987043682253571 2117 58-59 -0.03019887822790679 2117 60-61 -0.24310213116944368 2117 62-63 -0.3432745330096125 2117 64-65 -0.4836493091447309 2117 66-67 -0.32273503557861716 2117 68-69 -0.6571609007785284 2117 70-71 -0.24921526429873353 2117 72-73 -1.9861207582517189 2117 74-75 -0.4344087686560485 2117 76 -0.625788608241713 2118 1 -0.4679718560111077 2118 2 -0.5995845164547724 2118 3 -0.47636489580667174 2118 4 -1.2721731492058446 2118 5 -0.5278636711427005 2118 6 -0.32868924329061855 2118 7 -0.8363886258490325 2118 8 -0.1302463261085478 2118 9 -0.3909795229901434 2118 10-11 -1.595843595021762 2118 12-13 -0.5004206790922865 2118 14-15 -0.5882061982065423 2118 16-17 -0.3628520851458603 2118 18-19 -0.39692223022528594 2118 20-21 -0.3829361157461477 2118 22-23 -0.4838715062900718 2118 24-25 -0.13929821219664973 2118 26-27 -0.550722693648332 2118 28-29 -0.4457791777885802 2118 30-31 -0.3832131769898268 2118 32-33 -0.6327791912058345 2118 34-35 -0.6491347530740867 2118 36-37 -0.24259898251030165 2118 38-39 -0.42514799544515114 2118 40-41 -0.8278357837865826 2118 42-43 -0.324912128136873 2118 44-45 -0.6074881758145878 2118 46-47 -0.508291795304018 2118 48-49 -0.40817887953706844 2118 50-51 -0.5837849152882129 2118 52-53 -0.506060295292805 2118 54-55 -0.41724423382521536 2118 56-57 -0.1379930887640839 2118 58-59 -0.3737805280595623 2118 60-61 -0.40644388201119597 2118 62-63 -0.4635817720668527 2118 64-65 -0.3950339892794119 2118 66-67 -0.07677543961901989 2118 68-69 -0.014505176872802394 2118 70-71 -0.020027553861019953 2118 72-73 -0.2999886033695631 2118 74-75 -0.4417951322924054 2118 76 -0.21716066211376628 2119 1 0.4805303415368911 2119 2 0.16746093809068086 2119 3 -0.028469915468946283 2119 4 0.21999085727113865 2119 5 0.38965599112886906 2119 6 0.1957729357517124 2119 7 0.8627930766723608 2119 8 0.4576555938544402 2119 9 0.46441603564969114 2119 10-11 0.5062917855010269 2119 12-13 0.37820816661670875 2119 14-15 0.5360709241621819 2119 16-17 0.5577253495060006 2119 18-19 0.17777994419063248 2119 20-21 0.07570805496864352 2119 22-23 -0.20510097656534043 2119 24-25 0.4717791646250049 2119 26-27 0.4353763695022508 2119 28-29 0.36903403063149653 2119 30-31 0.17063397776382772 2119 32-33 0.1373240357199137 2119 34-35 0.3480098802823832 2119 36-37 0.3440017577163985 2119 38-39 0.20760848153842204 2119 40-41 0.14025639756895458 2119 42-43 -0.055235399013582764 2119 44-45 -0.042335214907801344 2119 46-47 0.16507594700455996 2119 48-49 0.48803757894084043 2119 50-51 0.10832704399931714 2119 52-53 0.16496532349044202 2119 54-55 0.29162402895526185 2119 56-57 0.0428492082518801 2119 58-59 0.1751799739066655 2119 60-61 0.09533439696173929 2119 62-63 0.0462999651526701 2119 64-65 0.09024099337152336 2119 66-67 -0.4732015326100054 2119 68-69 -0.4273333633174019 2119 70-71 0.1264491405641479 2119 72-73 0.04543847273499324 2119 74-75 0.11101107867219895 2119 76 0.4111212023322217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 25.0 13 448.0 14 1470.0 15 2244.0 16 2917.0 17 3517.0 18 3721.0 19 3568.0 20 2827.0 21 2203.0 22 1854.0 23 1738.0 24 1688.0 25 1885.0 26 2168.0 27 2476.0 28 2983.0 29 3518.0 30 4314.0 31 5465.0 32 6702.0 33 8706.0 34 11779.0 35 16829.0 36 31004.0 37 90970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08569216803904 16.062040079070687 11.148690679703806 29.703577073186466 2 23.420774948046503 16.78593315854226 29.486547384815292 30.30674450859594 3 22.513028693075785 17.147188520926544 23.500951520820564 36.8388312651771 4 18.777445500665834 16.114108772883732 23.965883485929933 41.142562240520505 5 21.057409190815633 28.447477433059476 26.237553047861727 24.257560328263164 6 37.724459844899805 25.365748015169036 13.995419798084056 22.914372341847102 7 34.29253390224909 21.485017578943975 16.40440326051396 27.81804525829298 8 29.17321365213505 27.308877942687577 16.109040138972727 27.408868266204657 9 25.73345436616733 14.587528395869523 16.327452182046898 43.35156505591625 10-11 32.05012418153082 18.78550923643334 19.57137788508946 29.592988696946378 12-13 29.446689490878764 16.339202197022406 20.198506135350957 34.01560217674787 14-15 28.359928301869402 17.363757424396717 17.957709161786187 36.3186051119477 16-17 30.443667764105236 18.806057492264554 18.39964427323686 32.35063047039335 18-19 29.80863603061455 17.980287621935204 18.906465272945937 33.30461107450431 20-21 29.320204035554166 18.796337681606847 19.192843088917662 32.690615193921325 22-23 30.629293939296197 18.338087097561985 18.477704922565096 32.55491404057672 24-25 30.203298298321364 18.649577690638232 17.78675796351505 33.36036604752535 26-27 29.369575685246325 19.16869604484369 19.024239645563437 32.43748862434655 28-29 29.983964685445187 18.810622013537863 19.537970979766936 31.667442321250018 30-31 29.951940134825662 18.124743688398816 19.328544242262268 32.59477193451325 32-33 28.8628750213113 19.13800968569862 19.81144681851065 32.18766847447943 34-35 29.40637081204123 18.639210030365724 19.941157768142254 32.01326138945079 36-37 29.348081522064685 17.74574810732602 20.3818985259491 32.5242718446602 38-39 28.273991917832834 17.99526313121772 21.96123877412785 31.7695061768216 40-41 28.236207555950806 17.86232668727911 21.99003783044037 31.91142792632971 42-43 27.412784937863155 17.645988176259443 22.118596817819476 32.822630068057926 44-45 26.278793296501263 17.945267969459177 23.62236834223416 32.1535703918054 46-47 26.612631957276022 18.399832274296035 23.439897521438017 31.547638246989923 48-49 26.1988471161777 18.65579828680174 23.127946143460772 32.017408453559796 50-51 25.4429294860866 19.795088954525138 22.74779860013547 32.01418295925279 52-53 25.28695379709798 19.675515272715543 22.881426221425578 32.1561047087609 54-55 25.166688937936883 19.361720755134296 22.586754277235844 32.88483602969298 56-57 25.0634731201128 19.764677151059114 22.606107243077858 32.56574248575023 58-59 25.613650291907238 19.65593191442303 22.194395934034034 32.5360218596357 60-61 25.445694195492603 18.905313310693437 22.410964837504206 33.23802765630976 62-63 25.313218536454997 19.412407094244337 22.222964597896055 33.05140977140461 64-65 25.84680745181342 19.40710806788283 21.674860958156124 33.071223522147626 66-67 25.786444629782373 19.812368388312652 20.55699678832924 33.844190193575734 68-69 25.48463051962713 20.659982213702822 20.828168702567954 33.02721856410209 70-71 25.982969390059026 20.886227600094003 20.94682081457555 32.183982195271426 72-73 26.08987148709111 20.497555536100194 20.56897719575525 32.843595781053445 74-75 25.52932665502417 21.16177697089222 20.83346772892946 32.47542864515415 76 25.766170094138356 21.1394289031937 19.730809460835587 33.363591541832356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 0.5 14 1.5 15 3.5 16 5.5 17 6.0 18 5.0 19 9.5 20 23.5 21 32.0 22 39.5 23 57.0 24 85.5 25 104.0 26 175.0 27 276.5 28 401.0 29 495.0 30 589.5 31 804.0 32 1116.0 33 1308.0 34 1484.0 35 1905.0 36 2306.5 37 2463.0 38 2813.5 39 3540.5 40 4216.5 41 4789.0 42 5062.0 43 5101.5 44 5493.0 45 5813.5 46 5782.0 47 5853.0 48 5677.5 49 5142.5 50 4854.0 51 4568.0 52 4020.0 53 3654.5 54 3551.0 55 3621.5 56 3706.5 57 4092.0 58 4463.0 59 4853.0 60 5674.0 61 6531.0 62 6957.0 63 7626.0 64 8698.5 65 9394.0 66 10042.5 67 10399.0 68 10746.5 69 11052.5 70 10938.5 71 10866.0 72 10614.5 73 9832.0 74 8605.5 75 7910.0 76 7165.5 77 5443.5 78 3706.0 79 2946.0 80 2313.5 81 1268.0 82 626.0 83 397.0 84 256.0 85 82.0 86 34.5 87 20.0 88 14.5 89 9.0 90 8.5 91 6.5 92 5.0 93 5.0 94 4.0 95 6.0 96 9.0 97 8.0 98 7.0 99 29.0 100 51.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3039245050018203E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.3039245050018203E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 217021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.62687765211982 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52655367139542 52.586781119645096 2 15.616238132357227 21.746198036560095 3 5.100821228200101 10.654627667436719 4 1.7594122347693177 4.900095216397039 5 0.754026487957851 2.625025501174945 6 0.3953233675750788 1.6515079048304404 7 0.23679436945631993 1.1541076813592235 8 0.17640119683903355 0.9825811639999115 9 0.10169674445199572 0.63727441052202 >10 0.33207241369491847 3.0351419383949008 >50 6.601533027272911E-4 0.026659359679568825 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.005990203713004732 0.0 2 0.0 0.0 0.0 0.006450988614005096 0.0 3 0.0 4.60784901000364E-4 0.0 0.00921569802000728 0.0 4 0.0 4.60784901000364E-4 0.0 0.04239221089203349 0.0 5 0.0 4.60784901000364E-4 0.0 0.05068633911004004 0.0 6 0.0 4.60784901000364E-4 0.0 0.05068633911004004 0.0 7 0.0 4.60784901000364E-4 0.0 0.05160790891204077 0.0 8 0.0 4.60784901000364E-4 0.0 0.0525294787140415 0.0 9 0.0 4.60784901000364E-4 0.0 0.05851968242704623 0.0 10 0.0 4.60784901000364E-4 0.0 0.0626667465360495 0.0 11 0.0 4.60784901000364E-4 0.0 0.06358831633805023 0.0 12 0.0 4.60784901000364E-4 0.0 0.06727459554605315 0.0 13 0.0 4.60784901000364E-4 0.0 0.06819616534805388 0.0 14 0.0 4.60784901000364E-4 0.0 0.07096087475405606 9.21569802000728E-4 15 0.0 4.60784901000364E-4 0.0 0.07464715396205897 9.21569802000728E-4 16 0.0 4.60784901000364E-4 0.0 0.07833343317006189 9.21569802000728E-4 17 0.0 4.60784901000364E-4 0.0 0.07925500297206262 9.21569802000728E-4 18 0.0 4.60784901000364E-4 0.0 0.08155892747706443 9.21569802000728E-4 19 0.0 4.60784901000364E-4 0.0 0.0857059915860677 9.21569802000728E-4 20 0.0 4.60784901000364E-4 0.0 0.09307855000207353 9.21569802000728E-4 21 0.0 4.60784901000364E-4 0.0 0.09768639901207717 9.21569802000728E-4 22 0.0 4.60784901000364E-4 0.0 0.10183346312108045 9.21569802000728E-4 23 0.0 4.60784901000364E-4 0.0 0.10551974232908336 9.21569802000728E-4 24 0.0 4.60784901000364E-4 0.0 0.11243151584408882 9.21569802000728E-4 25 0.0 0.001843139604001456 0.0 0.11980407426009465 9.21569802000728E-4 26 0.0 0.001843139604001456 0.0 0.1317844816861041 9.21569802000728E-4 27 0.0 0.001843139604001456 0.0 0.20320614134116052 9.21569802000728E-4 28 0.0 0.001843139604001456 0.0 0.3174807967892508 9.21569802000728E-4 29 0.0 0.001843139604001456 0.0 0.45802019159436186 9.21569802000728E-4 30 0.0 0.001843139604001456 0.0 0.6022458656074757 9.21569802000728E-4 31 0.0 0.001843139604001456 0.0 0.7510793886305933 9.21569802000728E-4 32 0.0 0.001843139604001456 0.0 0.9303247151197349 9.21569802000728E-4 33 0.0 0.001843139604001456 0.0 1.155648531708913 9.21569802000728E-4 34 0.0 0.001843139604001456 0.0 1.4781979624091677 9.21569802000728E-4 35 0.0 0.001843139604001456 0.0 1.906267135438506 9.21569802000728E-4 36 0.0 0.001843139604001456 0.0 2.5057482916399794 9.21569802000728E-4 37 0.0 0.001843139604001456 0.0 3.2563668953695726 9.21569802000728E-4 38 0.0 0.001843139604001456 0.0 4.080250298358224 9.21569802000728E-4 39 0.0 0.001843139604001456 0.0 4.922565097386888 9.21569802000728E-4 40 0.0 0.001843139604001456 0.0 5.785154432059571 9.21569802000728E-4 41 0.0 0.001843139604001456 0.0 6.691057547426286 9.21569802000728E-4 42 0.0 0.001843139604001456 0.0 7.592813598683998 9.21569802000728E-4 43 0.0 0.001843139604001456 0.0 8.44342252593067 9.21569802000728E-4 44 0.0 0.001843139604001456 0.0 9.259933370503315 9.21569802000728E-4 45 0.0 0.001843139604001456 0.0 10.078748139580963 9.21569802000728E-4 46 0.0 0.001843139604001456 0.0 10.913229595292622 9.21569802000728E-4 47 0.0 0.001843139604001456 0.0 11.727897300261265 9.21569802000728E-4 48 0.0 0.001843139604001456 0.0 12.510310062159883 9.21569802000728E-4 49 0.0 0.001843139604001456 0.0 13.26553651489948 9.21569802000728E-4 50 0.0 0.001843139604001456 0.0 14.007860990411066 9.21569802000728E-4 51 0.0 0.001843139604001456 0.0 14.716087383248626 9.21569802000728E-4 52 0.0 0.001843139604001456 0.0 15.439980462720198 9.21569802000728E-4 53 0.0 0.001843139604001456 0.0 16.12747153501274 9.21569802000728E-4 54 0.0 0.00230392450500182 0.0 16.78547237364126 9.21569802000728E-4 55 0.0 0.00230392450500182 0.0 17.414443763506757 9.21569802000728E-4 56 0.0 0.00230392450500182 0.0 18.040650443966253 9.21569802000728E-4 57 0.0 0.00230392450500182 0.0 18.70694541081278 9.21569802000728E-4 58 0.0 0.00230392450500182 0.0 19.364024679639297 9.21569802000728E-4 59 0.0 0.00230392450500182 0.0 19.98792743559379 9.21569802000728E-4 60 0.0 0.00230392450500182 0.0 20.65698711184632 9.21569802000728E-4 61 0.0 0.00230392450500182 0.0 21.27628201879081 9.21569802000728E-4 62 0.0 0.00230392450500182 0.0 21.941655415835335 9.21569802000728E-4 63 0.0 0.00230392450500182 0.0 22.60518567327586 9.21569802000728E-4 64 0.0 0.00230392450500182 0.0 23.225402150022347 9.21569802000728E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAGTG 20 0.006944477 52.500004 5 CATTAAA 75 1.4844609E-6 32.666668 61 GCTCTAC 60 4.9711374E-4 29.166666 1 GTATCAT 50 0.007197688 27.999998 57 GACTCTT 50 0.007197688 27.999998 11 TAGGCTT 65 7.938435E-4 26.923077 4 TAATATA 70 0.0012233502 25.0 7 CGTATCA 70 0.0012233502 25.0 56 TATCATT 75 0.0018282657 23.333334 58 CCGTATC 105 2.0307612E-5 23.333334 55 TCATTAA 80 0.0026602477 21.875002 60 TAAATCT 80 0.0026602477 21.875002 39 ATCATTA 100 3.919465E-4 21.0 59 AAAACAA 135 5.98764E-6 20.74074 68 ACTTCAA 85 0.0037810088 20.588236 20 ATCCGCG 120 5.69036E-5 20.416666 35 AAGTGTA 220 3.4560799E-9 19.090908 70 GAAGTGT 250 8.5310603E-10 18.2 69 CACGCGA 100 0.009665813 17.5 2 CATCTTA 100 0.009665813 17.5 9 >>END_MODULE