FastQCFastQC Report
Wed 25 May 2016
SRR1780166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217021
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT113915.248800807295146No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC30221.3924919708231No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG28261.3021781302270286No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG25641.1814524861649334TruSeq Adapter, Index 12 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6880.3170200118882504No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4070.18753945470714814TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2610.120264859161095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT207.914154E-570.0000139
ATATCGT207.914154E-570.0000137
CGTATCG150.002222466570.038
AATATAC150.002222466570.069
CCGTATC150.002222466570.037
TATCGTA252.8332288E-670.038
TCTTTAT1350.059.6296271
TTAATAC509.767973E-1055.9999963
CTTTATA1450.055.5172422
AACCGTA200.00694447752.50000435
TGCCAAC200.00694447752.5000041
CTATCGG200.00694447752.50000434
TCCTATA200.00694447752.50000427
GACCCTA200.00694447752.50000418
ACAATAA200.00694447752.50000470
ACCGTAT200.00694447752.50000436
GGACACT200.00694447752.50000456
TATACAA200.00694447752.50000430
TATCGGC200.00694447752.50000440
ATACAAC200.00694447752.50000431
Produced by FastQC (version 0.11.5)