FastQCFastQC Report
Wed 25 May 2016
SRR1780165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538954
Sequences flagged as poor quality0
Sequence length50
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAT250.002358685735.17853531
CTAATTG250.002358685735.17853533
TATATCA353.2393524E-431.3890112
GAGCTAT300.00577767729.2964126
TCTATGG300.00577767729.2964122
ATATCAC407.078964E-427.4653843
CAATTGG602.8457766E-422.02748318
TGTACCG500.002597526621.9723075
GAACAAT550.004452967620.02126139
CATGTAA550.00449628219.98780431
CTTTACA550.004513219519.9748271
AACACTA550.004513219519.9748275
GTAACGA708.052515E-418.87894434
TATGGCT708.1843586E-418.8334064
TGCTAAA600.0073537418.35623618
TGTAACG600.00743300218.32215533
ACTATGG600.007460908518.3102571
ATATATC600.007460908518.3102571
CACAGTG600.007460908518.3102577
TATACCA851.4428204E-418.0948415
Produced by FastQC (version 0.11.5)