FastQCFastQC Report
Wed 25 May 2016
SRR1780165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538954
Sequences flagged as poor quality0
Sequence length50
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT145712.703570249037951No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC39060.7247371760855287No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG35700.662394193196451No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG26870.4985583185206901Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC8020.14880676272928672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGGC254.441729E-543.99656714
CGCAAAG301.3007753E-436.66380312
TCTTTAT1500.035.2528531
TTAATAC250.002349329435.2070542
CTTTATA1500.035.2070542
GTCTTCT3350.032.17659843
ATGCCGT22450.032.1400344
TCTTCTG3600.029.94210844
TATGCCG25150.028.60214243
CCGTCTT3750.028.15780441
TTTATAC1900.027.7950423
CTTATTA406.965674E-427.5412921
GCAAAGG508.3374354E-526.3979413
TATACAA609.724765E-625.6718082
CGTCTTC4300.025.06781242
AAAGGCC450.00139784524.44253715
ATACAAC651.8081664E-523.6970563
TTGTATC30450.023.26255433
TGTATCT30500.023.22441934
GTATCTC30500.023.22441935
Produced by FastQC (version 0.11.5)