FastQCFastQC Report
Wed 25 May 2016
SRR1780164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64832
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGTGT150.002215416670.033
AGTACTA150.002215416670.021
ATATGAC252.3741802E-456.00000410
TTGGAGC200.006922617652.568
GTGTGTA200.006922617652.534
ACGTGAA200.006922617652.59
CATATGA350.00124702240.09
CGAACGA400.00240268235.025
TCATTAA400.00240268235.060
CATTAAA400.00240268235.061
ACGAACG400.00240268235.024
ACGACTG450.004278813531.11110932
TATCATT500.00716105128.00000258
TGACCGC500.00716105128.00000214
CCGACGG500.00716105128.00000223
GATCTCT500.00716105128.00000241
GACGAAC657.878138E-426.92307723
TAGATCT700.001214104825.039
GCGCCAC750.001814517423.3333346
CGACGAA1102.8689103E-522.27272622
Produced by FastQC (version 0.11.5)