##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64832 Sequences flagged as poor quality 0 Sequence length 76 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.357338968410662 34.0 23.0 34.0 12.0 34.0 2 28.509871668311945 34.0 23.0 34.0 12.0 34.0 3 28.329405231984204 34.0 23.0 34.0 11.0 34.0 4 28.301486920039487 34.0 23.0 34.0 11.0 34.0 5 28.152517275419545 34.0 23.0 34.0 11.0 34.0 6 30.258144126357355 36.0 25.0 38.0 11.0 38.0 7 30.881061821322803 37.0 26.0 38.0 12.0 38.0 8 30.735192497532083 36.0 26.0 38.0 11.0 38.0 9 31.323081194471865 37.0 27.0 38.0 12.0 38.0 10-11 30.943515547877592 37.0 26.0 38.0 11.0 38.0 12-13 31.1025728035538 37.0 26.5 38.0 11.0 38.0 14-15 30.964716498025666 37.0 26.0 38.0 11.0 38.0 16-17 30.98939566880553 37.0 26.0 38.0 11.0 38.0 18-19 31.044692435834158 37.0 26.0 38.0 11.0 38.0 20-21 31.03752776406713 37.0 26.0 38.0 11.0 38.0 22-23 30.992920162882527 37.0 26.0 38.0 11.0 38.0 24-25 30.975035476307994 37.0 26.0 38.0 11.0 38.0 26-27 30.888689227541953 37.0 26.0 38.0 11.0 38.0 28-29 30.928384131293186 37.0 26.0 38.0 11.0 38.0 30-31 30.96058273692004 37.0 26.0 38.0 11.0 38.0 32-33 31.044268262586378 37.0 26.0 38.0 11.0 38.0 34-35 30.9732847976308 37.0 26.0 38.0 11.0 38.0 36-37 30.85905879812438 37.0 26.0 38.0 11.0 38.0 38-39 30.804672075518262 37.0 26.0 38.0 11.0 38.0 40-41 30.88875863770977 37.0 26.0 38.0 11.0 38.0 42-43 30.94066201875617 37.0 26.0 38.0 11.0 38.0 44-45 31.05819656959526 37.0 26.0 38.0 11.0 38.0 46-47 30.923486858341562 37.0 26.0 38.0 11.0 38.0 48-49 30.9041059970385 37.0 26.0 38.0 11.0 38.0 50-51 30.757504010365253 37.0 26.0 38.0 11.0 38.0 52-53 30.859159057255674 37.0 26.0 38.0 11.0 38.0 54-55 30.801440646594273 37.0 26.0 38.0 11.0 38.0 56-57 30.69024555774926 37.0 25.5 38.0 11.0 38.0 58-59 30.749259624876604 37.0 25.5 38.0 11.0 38.0 60-61 30.78947124876604 37.0 26.0 38.0 11.0 38.0 62-63 30.56436636229023 37.0 25.0 38.0 11.0 38.0 64-65 30.490976678183614 37.0 25.0 38.0 11.0 38.0 66-67 30.430327615992105 36.5 25.0 38.0 11.0 38.0 68-69 30.29146100691017 36.0 25.0 38.0 11.0 38.0 70-71 30.20639499012833 36.0 24.0 38.0 11.0 38.0 72-73 30.258977048371175 36.0 24.0 38.0 11.0 38.0 74-75 30.254241732477787 36.0 24.0 38.0 11.0 38.0 76 28.062422877591313 33.0 20.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.9265641667124243 1101 2 -0.7089741492204418 1101 3 -0.37893000690100465 1101 4 -0.18336280482906986 1101 5 -1.2292538976208327 1101 6 -0.08588778483010984 1101 7 -0.2355805944868976 1101 8 -1.758467540463183 1101 9 -0.2510357283907183 1101 10-11 -1.1524683599506105 1101 12-13 -0.1596999047145573 1101 14-15 -0.8985373109745254 1101 16-17 -0.7048355128251949 1101 18-19 -0.6279969792233118 1101 20-21 -0.5355373936418175 1101 22-23 -1.0160765881775582 1101 24-25 -0.6255243719667298 1101 26-27 -1.109307133033088 1101 28-29 -0.8160679798900787 1101 30-31 -0.9766786419995377 1101 32-33 -1.2570206889849302 1101 34-35 -0.8126497963122858 1101 36-37 -1.0054723600908275 1101 38-39 -1.213683338881662 1101 40-41 -0.3688508537443731 1101 42-43 -1.1479691344384122 1101 44-45 -0.8180946054251059 1101 46-47 0.05448097119401396 1101 48-49 0.0030709287992962686 1101 50-51 -0.5811826026218831 1101 52-53 -0.14061108317621418 1101 54-55 -0.7669131529653406 1101 56-57 -0.4828773491006153 1101 58-59 -0.27178498065160994 1101 60-61 -1.100713729474755 1101 62-63 -0.9192143775934625 1101 64-65 -0.6989695621787995 1101 66-67 -0.991484906805745 1101 68-69 -1.2484223665600709 1101 70-71 -0.07586165157691482 1101 72-73 -0.837831785523921 1101 74-75 -0.7871953877646582 1101 76 -1.7211553185232482 1102 1 0.4684134509314255 1102 2 -0.42863786796702996 1102 3 0.06258107509484745 1102 4 0.0011114827569080887 1102 5 -0.41995157203943734 1102 6 0.24724902049798203 1102 7 -1.304256214096025 1102 8 -0.24411007785809602 1102 9 -0.6716930053860217 1102 10-11 -0.6651990074012062 1102 12-13 0.004499243664085384 1102 14-15 0.11125633461996998 1102 16-17 -0.389724692866686 1102 18-19 -0.12067085191684157 1102 20-21 -0.7072482791096668 1102 22-23 -0.7955175751848707 1102 24-25 -0.6742960719339735 1102 26-27 -0.9806577171579924 1102 28-29 -1.0091239903715739 1102 30-31 -0.7292389651789257 1102 32-33 -0.36071104819444955 1102 34-35 -0.9920580286520639 1102 36-37 -0.7263807562039375 1102 38-39 -0.7250601267405976 1102 40-41 -0.6537072791183185 1102 42-43 -0.13621020879150336 1102 44-45 0.035874168458509104 1102 46-47 -0.36606712357615834 1102 48-49 -0.5994511806012923 1102 50-51 -0.6534612356604228 1102 52-53 -0.4038494279736824 1102 54-55 -1.0426856172081607 1102 56-57 -0.8675918375818874 1102 58-59 -0.47204701799192605 1102 60-61 -0.2678826343770382 1102 62-63 -0.6896696674722733 1102 64-65 -0.2859877956103922 1102 66-67 -0.37781529221899746 1102 68-69 -0.18255273013687656 1102 70-71 -0.4422444444582325 1102 72-73 -0.3140737333348156 1102 74-75 -0.518148548590073 1102 76 -0.26462076234899357 1103 1 0.21080245727391755 1103 2 1.3771760855213415 1103 3 0.6062385803802606 1103 4 1.2248958379366144 1103 5 0.7571097387428054 1103 6 0.4626824243034804 1103 7 0.8636084793500629 1103 8 1.3758687804928584 1103 9 1.8146706309776164 1103 10-11 1.0244415845649684 1103 12-13 0.9576176368987603 1103 14-15 0.838370499524828 1103 16-17 1.0082139963510706 1103 18-19 1.166379888040879 1103 20-21 0.3441787822011193 1103 22-23 0.2758354903542397 1103 24-25 1.0246996997362139 1103 26-27 0.6273232553578616 1103 28-29 0.859956347894812 1103 30-31 0.35263207076187797 1103 32-33 0.5871747869006896 1103 34-35 0.8918743180709825 1103 36-37 0.7832386941131873 1103 38-39 0.46558469355538534 1103 40-41 1.590901600374277 1103 42-43 0.918388099876573 1103 44-45 0.4040031325177047 1103 46-47 1.0682880561278623 1103 48-49 0.6069441682359162 1103 50-51 0.49659161846219746 1103 52-53 0.7999032147324492 1103 54-55 0.5534560318404615 1103 56-57 0.9739515027986592 1103 58-59 0.8045914598093376 1103 60-61 1.3763562662572184 1103 62-63 0.49547832407106895 1103 64-65 0.9988430711972178 1103 66-67 0.7666350249057388 1103 68-69 0.8893817307510687 1103 70-71 1.1879881136812998 1103 72-73 1.6343406429865368 1103 74-75 1.285339823502948 1103 76 1.4426731065727836 1104 1 -0.5202819291916327 1104 2 -0.33964005797641406 1104 3 -1.1429460914501526 1104 4 -0.18197330189216743 1104 5 -0.892931027665675 1104 6 -0.9463269519704731 1104 7 -1.0868195679389707 1104 8 -0.19822416691847877 1104 9 -2.1488230502290726 1104 10-11 -0.5344658756877863 1104 12-13 -0.6910268800193009 1104 14-15 -0.8737473153956756 1104 16-17 -1.266521021993814 1104 18-19 -1.6218284283064506 1104 20-21 -1.3812288818836755 1104 22-23 -1.5828885210603545 1104 24-25 -1.5713969166518815 1104 26-27 -1.6662662269087498 1104 28-29 -1.3745218420766498 1104 30-31 -0.5663752671916491 1104 32-33 -0.9833307165644563 1104 34-35 -1.0384966562461813 1104 36-37 -1.7452774086181648 1104 38-39 -0.9030557467218294 1104 40-41 -1.3003336218026469 1104 42-43 -1.1069787977997443 1104 44-45 -0.9189177806498456 1104 46-47 -0.5019728488864104 1104 48-49 -1.0158136792977182 1104 50-51 -0.5192970892426523 1104 52-53 -1.7548766466617636 1104 54-55 -0.9595382608623488 1104 56-57 -1.6065010043354597 1104 58-59 -1.1923306763504655 1104 60-61 -1.0635234728377725 1104 62-63 -0.9471939140047567 1104 64-65 -0.7036538713221496 1104 66-67 -0.9726739844705357 1104 68-69 -1.480216720125405 1104 70-71 -1.6245679401303583 1104 72-73 -0.2883254801280195 1104 74-75 -0.6628664545239573 1104 76 -0.38584299615856565 1105 1 0.14781544428690552 1105 2 -0.47282391447865635 1105 3 -0.3736315494898683 1105 4 -0.1238054607646859 1105 5 0.7040794357125009 1105 6 0.17869974032079483 1105 7 0.33417124991283487 1105 8 -0.6698022151781409 1105 9 0.4664022327092141 1105 10-11 0.3075584676818508 1105 12-13 -0.1368628825817595 1105 14-15 0.4661843523386864 1105 16-17 0.2362442993813758 1105 18-19 0.15479980146078987 1105 20-21 0.41268527570761293 1105 22-23 -0.044077929559183104 1105 24-25 -0.3541964041598895 1105 26-27 -0.3556854026507956 1105 28-29 -0.1410176780792156 1105 30-31 0.09927709240689708 1105 32-33 0.08197998170588505 1105 34-35 -0.3537966776000161 1105 36-37 -0.03645827558378656 1105 38-39 0.33474053771123025 1105 40-41 0.6590834185560972 1105 42-43 0.06849632498479608 1105 44-45 -0.12835617484160267 1105 46-47 -0.0020582642184621136 1105 48-49 0.04861083490258267 1105 50-51 0.410769107639684 1105 52-53 -0.4577807679515331 1105 54-55 0.2518326552170862 1105 56-57 -0.15397057512342016 1105 58-59 0.15004600526388856 1105 60-61 -0.39756148266053515 1105 62-63 0.18779434138708595 1105 64-65 -0.08438203702789338 1105 66-67 0.051808185078897395 1105 68-69 -0.19600787963854316 1105 70-71 0.5808452565384457 1105 72-73 0.723301681947575 1105 74-75 0.573435061598186 1105 76 0.5848808987805754 1106 1 -0.022524081658001904 1106 2 -0.09300328667596602 1106 3 0.587995612585722 1106 4 0.015336115148190999 1106 5 0.932181079957644 1106 6 1.197170805877061 1106 7 0.35264231546910096 1106 8 0.26365924115327033 1106 9 0.6538675327305725 1106 10-11 -0.28558695192096906 1106 12-13 0.35381616930805393 1106 14-15 0.25853968012002326 1106 16-17 0.5204744937012578 1106 18-19 0.2570953385096928 1106 20-21 0.2622688759638585 1106 22-23 0.20410699463471005 1106 24-25 0.5496793165319751 1106 26-27 -0.21110927623610465 1106 28-29 -0.1368536806416749 1106 30-31 0.25201038303256595 1106 32-33 0.8238441713279201 1106 34-35 0.2529831643816216 1106 36-37 -0.03227292431323292 1106 38-39 -0.20350417721509828 1106 40-41 0.4485453019641703 1106 42-43 0.5656669421105711 1106 44-45 0.7470015018613658 1106 46-47 0.48233208384901616 1106 48-49 0.4640069485049736 1106 50-51 0.2833934937169893 1106 52-53 -0.014966332760415924 1106 54-55 0.16602227725423901 1106 56-57 -0.0011198384161765773 1106 58-59 0.07672761961366348 1106 60-61 0.37818056303666836 1106 62-63 -0.32163337588816177 1106 64-65 0.18199635289309768 1106 66-67 0.32553229355637114 1106 68-69 0.3987070466907383 1106 70-71 0.2364186069548424 1106 72-73 0.38656241224866505 1106 74-75 0.22054161722492083 1106 76 0.8428565554109397 1107 1 0.41030698118602515 1107 2 0.06224717556873571 1107 3 0.2564970896435099 1107 4 0.11207808696720534 1107 5 0.17358970427490306 1107 6 -0.6765248139558935 1107 7 -0.5874040152643261 1107 8 -0.1593531066247671 1107 9 -0.6991811570447908 1107 10-11 -0.5044252831644584 1107 12-13 0.23222987300388453 1107 14-15 -0.19524647591678956 1107 16-17 0.14706578437347773 1107 18-19 0.5754384834738815 1107 20-21 0.19393104415717133 1107 22-23 0.36344858815690984 1107 24-25 0.09004868724160175 1107 26-27 0.1695572062411017 1107 28-29 0.5465246374251862 1107 30-31 -0.28805082454187314 1107 32-33 0.5476874969394672 1107 34-35 -0.1393191330276835 1107 36-37 0.873652308934382 1107 38-39 -0.043323104807388546 1107 40-41 -0.07622463831593862 1107 42-43 0.06753717355171318 1107 44-45 0.7240699140773756 1107 46-47 -0.15680888484585864 1107 48-49 -0.103363888636121 1107 50-51 -0.08810247890962586 1107 52-53 0.09444220654632218 1107 54-55 -0.1233535330022768 1107 56-57 -0.18328675657230065 1107 58-59 0.3919891332259695 1107 60-61 -0.001465793199919574 1107 62-63 -0.43273149928093346 1107 64-65 0.10770778468278408 1107 66-67 -0.1073093956026625 1107 68-69 0.2650280039091939 1107 70-71 0.013106597093624828 1107 72-73 -0.4734146349109203 1107 74-75 -0.24143742768188403 1107 76 0.1454089962754992 1108 1 -0.4412177447462824 1108 2 -0.052621894276636993 1108 3 -0.012448288306607935 1108 4 0.3546938177345922 1108 5 -0.39087005923699536 1108 6 0.42833898996004294 1108 7 -0.32022990448831834 1108 8 0.004656659280733777 1108 9 0.17477164107862464 1108 10-11 -0.24070993058654722 1108 12-13 -1.1042510499699283 1108 14-15 0.21741090356523785 1108 16-17 -0.30516984203276465 1108 18-19 0.19491524157623275 1108 20-21 -0.3757707127483769 1108 22-23 -0.11658875207000463 1108 24-25 0.2981845482210659 1108 26-27 -0.48151512848052036 1108 28-29 -0.6099931470546842 1108 30-31 -0.20014570701590273 1108 32-33 -0.3911080413821395 1108 34-35 -0.45762063142397125 1108 36-37 -0.20261989174539963 1108 38-39 -0.5200213670506741 1108 40-41 -1.3267751673024861 1108 42-43 -0.29550078901231913 1108 44-45 -0.011400907886336853 1108 46-47 -0.9195907317509295 1108 48-49 -0.44734876105701105 1108 50-51 -0.4268932300226531 1108 52-53 -0.2796422398130076 1108 54-55 -0.9074025540181232 1108 56-57 -0.8119070830599249 1108 58-59 -0.5221762169583357 1108 60-61 -0.43753262263167514 1108 62-63 -0.43002672643397943 1108 64-65 -0.11151046415632138 1108 66-67 0.29112997440068966 1108 68-69 0.33761405398339406 1108 70-71 -0.05039572470253617 1108 72-73 -0.8242452155993227 1108 74-75 -0.5389026007394726 1108 76 -0.24470063080095272 1109 1 0.3035297300011912 1109 2 0.857176085521342 1109 3 0.6085113076529858 1109 4 0.09262311066388662 1109 5 0.29074610237916687 1109 6 0.276318787939843 1109 7 0.05179029753188402 1109 8 -0.5400403104162343 1109 9 1.0383069946139756 1109 10-11 0.6164870391104245 1109 12-13 0.5467085459896666 1109 14-15 0.6767795904339238 1109 16-17 0.32207763271470924 1109 18-19 0.5020617062226975 1109 20-21 0.5405424185647512 1109 22-23 0.5590173085360597 1109 24-25 0.0015178815543954727 1109 26-27 0.5855050735396823 1109 28-29 0.07291089334935563 1109 30-31 0.8430866162164214 1109 32-33 0.41376569599160007 1109 34-35 0.6259652271618918 1109 36-37 0.8468750577495499 1109 38-39 1.0658119662826557 1109 40-41 0.5540834185561003 1109 42-43 -0.018884627396154485 1109 44-45 0.2060485870631581 1109 46-47 0.22556078340058505 1109 48-49 0.3269441682359151 1109 50-51 0.048864345734923376 1109 52-53 0.5662668510960849 1109 54-55 0.40254694093136933 1109 56-57 0.43031513916229613 1109 58-59 0.9025460052638863 1109 60-61 0.6486289935299396 1109 62-63 0.7618419604347046 1109 64-65 0.7015703439244874 1109 66-67 1.1654986612693747 1109 68-69 1.0475635489328887 1109 70-71 0.9529881136813003 1109 72-73 1.3061588248047187 1109 74-75 1.0853398235029523 1109 76 0.0613094702091459 1110 1 -0.3783873684444039 1110 2 0.13702064510683343 1110 3 -0.44239542809830823 1110 4 -0.191755127678082 1110 5 -0.612673586740005 1110 6 0.5346089433802561 1110 7 0.026868017739136718 1110 8 -0.5453511912452491 1110 9 0.1552499997953234 1110 10-11 -0.5702357588170415 1110 12-13 -0.2142500032331327 1110 14-15 -0.39364787070597274 1110 16-17 -0.3161736626220808 1110 18-19 0.06759279430559673 1110 20-21 -0.2651829700362818 1110 22-23 -0.08966144794062458 1110 24-25 -0.29982926870466997 1110 26-27 -0.2652462217971099 1110 28-29 -0.20171345898225113 1110 30-31 -0.025877114353523467 1110 32-33 0.5120688047998883 1110 34-35 0.4113279214624086 1110 36-37 0.14972479868219324 1110 38-39 0.12548813208576703 1110 40-41 -0.18780777315374664 1110 42-43 -0.3774079434583335 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-0.9194575814352461 2116 22-23 -1.1101493581306094 2116 24-25 -0.9476487851122677 2116 26-27 -0.08782825979364972 2116 28-29 -1.052922439983977 2116 30-31 -0.25608005045024385 2116 32-33 -0.8162343040084039 2116 34-35 -0.603201439504776 2116 36-37 -0.9197916089171159 2116 38-39 -0.5116880337173448 2116 40-41 -0.3450832481105657 2116 42-43 -0.3630512940628208 2116 44-45 -1.391451412936842 2116 46-47 -0.5419392165994132 2116 48-49 -0.022222498430750193 2116 50-51 -0.5094689875984066 2116 52-53 -0.4428998155705841 2116 54-55 -0.5124530590686298 2116 56-57 -1.1521848608377034 2116 58-59 0.30004600526388714 2116 60-61 -0.4713710064700578 2116 62-63 -0.6056580395652951 2116 64-65 -0.9492629894088473 2116 66-67 -0.2803346720639581 2116 68-69 -1.0924364510671083 2116 70-71 -0.4453452196520331 2116 72-73 -0.31300784186194974 2116 74-75 -0.8729935098303834 2116 76 -0.32702386312418597 2117 1 -0.4753988414273813 2117 2 -0.3138953430500848 2117 3 0.13708273622441425 2117 4 -0.5130911750503984 2117 5 -0.30961104047797505 2117 6 -0.9343954977744424 2117 7 0.31036172610330937 2117 8 0.2403168324409073 2117 9 0.037235566042550516 2117 10-11 -0.18172724660386308 2117 12-13 0.6479585459896668 2117 14-15 -0.4216132667089312 2117 16-17 -0.90185093871386 2117 18-19 0.19974027765126579 2117 20-21 0.10822098999332397 2117 22-23 0.1850887371074883 2117 24-25 0.5422321672686827 2117 26-27 1.1044336449682497 2117 28-29 -0.37762482093635796 2117 30-31 0.22576518764499198 2117 32-33 0.06055141027731281 2117 34-35 0.030429512876175124 2117 36-37 0.5772322006066872 2117 38-39 0.20170482342551566 2117 40-41 -0.11145229572961668 2117 42-43 -0.06692034168187178 2117 44-45 0.041584301348873254 2117 46-47 0.39466792625773195 2117 48-49 0.5221227396644856 2117 50-51 0.6131500600206401 2117 52-53 1.2844811368103706 2117 54-55 -0.122274487640059 2117 56-57 -0.12539914655198814 2117 58-59 -0.6749539947361143 2117 60-61 0.19202185067279487 2117 62-63 -0.27887232527957906 2117 64-65 -0.5578939417897963 2117 66-67 1.2101415184122324 2117 68-69 -0.13618645106711114 2117 70-71 0.3594166851098741 2117 72-73 -0.9490197466238506 2117 74-75 -0.034303033639908165 2117 76 0.3563094702091476 2118 1 1.155978709593029 2118 2 0.5598291467458303 2118 3 1.073307226020333 2118 4 -0.515642195458561 2118 5 0.22992977584855367 2118 6 1.0528494001847406 2118 7 1.6573005016135163 2118 8 1.9533270365225377 2118 9 1.0181029129813268 2118 10-11 -0.26080887925692764 2118 12-13 0.2315044643570161 2118 14-15 1.0777489781890282 2118 16-17 1.7205980408779773 2118 18-19 0.2513984409165744 2118 20-21 1.0616648675443479 2118 22-23 1.290956084046261 2118 24-25 1.9223342080850152 2118 26-27 1.098056093947843 2118 28-29 1.2824517096758932 2118 30-31 1.07716824886948 2118 32-33 1.4738167163997602 2118 34-35 0.8499448189986261 2118 36-37 0.7315689353005688 2118 38-39 1.0275976805683733 2118 40-41 0.19785892876017996 2118 42-43 1.2385643521956808 2118 44-45 1.5957934850223445 2118 46-47 0.529234252788342 2118 48-49 0.4513319233379569 2118 50-51 0.21008883553084345 2118 52-53 0.6071852184430249 2118 54-55 1.3018326552170798 2118 56-57 0.48174371059086596 2118 58-59 0.6694337603659264 2118 60-61 0.2296494016932087 2118 62-63 0.3843929808428683 2118 64-65 -0.12485822750408104 2118 66-67 0.01881498779998836 2118 68-69 0.3274615081165635 2118 70-71 0.23186566470170789 2118 72-73 0.5681996411312475 2118 74-75 -0.37486425812970126 2118 76 0.2644727355152696 2119 1 -0.6292609676732255 2119 2 -0.9798006586647041 2119 3 -1.4862561342074763 2119 4 -0.329121075382627 2119 5 0.8172577302861441 2119 6 0.0751559972421667 2119 7 0.009697274276067702 2119 8 0.6279829453977186 2119 9 0.5399349015907191 2119 10-11 -0.8349664492616711 2119 12-13 -1.2175937795917307 2119 14-15 -0.7759529677056136 2119 16-17 -0.9932130649597077 2119 18-19 -0.2648568984284658 2119 20-21 -0.5991087442259406 2119 22-23 0.013784750396521162 2119 24-25 -0.45569142077118485 2119 26-27 -0.23879725204171365 2119 28-29 -0.3893565485111061 2119 30-31 -0.6850529186672993 2119 32-33 -0.4618738388921244 2119 34-35 0.20910476204561235 2119 36-37 -0.16010168643649791 2119 38-39 -0.1459903592987395 2119 40-41 -0.008358441909020797 2119 42-43 -0.7501636971635968 2119 44-45 0.17540905217943603 2119 46-47 -0.2707182863668578 2119 48-49 -0.7564279247873387 2119 50-51 -0.1011356542650752 2119 52-53 0.27056917667748337 2119 54-55 -0.05198794278955887 2119 56-57 -0.5780569538609583 2119 58-59 -0.7825121342709984 2119 60-61 -0.9085803087956421 2119 62-63 -0.9989719930536651 2119 64-65 -0.16970872584295194 2119 66-67 -0.1789780829166716 2119 68-69 -0.6616224975787404 2119 70-71 -0.5492211886442817 2119 72-73 -0.98151559379993 2119 74-75 -1.3297764555668188 2119 76 -0.8995044832792232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 14.0 13 236.0 14 1015.0 15 1803.0 16 2287.0 17 2500.0 18 2475.0 19 2031.0 20 1648.0 21 1170.0 22 830.0 23 740.0 24 636.0 25 700.0 26 745.0 27 863.0 28 986.0 29 1165.0 30 1463.0 31 1802.0 32 2333.0 33 2836.0 34 3764.0 35 5385.0 36 8912.0 37 16492.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.13963998704324 17.13377859357406 9.927349570421558 35.79923184896114 2 20.406589338598224 21.335143139190524 26.78152764067127 31.476739881539977 3 20.263141658440276 16.86975567620928 22.25135735439289 40.61574531095755 4 18.038931391905233 16.32835636722606 23.78917818361303 41.843534057255674 5 19.934600197433365 26.693608094768017 28.129627344521225 25.242164363277393 6 33.75185093780849 22.737228529121424 18.120681145113522 25.390239387956566 7 33.64079466929911 19.849765547877592 16.14634748272458 30.363092300098714 8 26.724457058242844 23.667324777887462 21.28732724580454 28.32089091806515 9 24.736241362290226 13.838845014807502 16.912944225074035 44.51196939782823 10-11 29.84120373583829 17.95423520973601 21.405489615387584 30.799071439038123 12-13 27.09541584402764 15.145298617966436 21.388357601184598 36.370927936821325 14-15 27.315985932872657 16.2905663869694 19.430990868706814 36.96245681145113 16-17 29.77773321816387 17.53686451135242 18.77390794669299 33.91149432379072 18-19 29.169237413622902 16.562037265547875 18.597297630799606 35.67142769002962 20-21 28.049420039486673 17.024771717670287 19.71248766041461 35.21332058242843 22-23 30.59368830207305 16.965387462981244 17.840726801579468 34.60019743336623 24-25 29.319626110562684 18.429942003948668 16.90368953603159 35.34674234945706 26-27 28.021655972359326 19.130984698914116 19.072371668311945 33.77498766041461 28-29 29.309600197433365 17.62864017769003 20.511475814412634 32.550283810463974 30-31 29.11602295162882 16.792633267522213 20.46057502467917 33.63076875616979 32-33 27.646069842053304 18.42068731490622 20.951073543928924 32.982169299111554 34-35 27.565091312931884 19.265177690029613 20.05259748272458 33.11713351431392 36-37 28.070243089832182 16.895977295162883 20.841559723593285 34.192219891411646 38-39 26.840911895360314 16.814998766041462 23.40125246791708 32.94283687068115 40-41 26.658131786771968 16.837364264560712 23.265517028627837 33.23898692003949 42-43 25.387925715695953 16.700857601184598 22.895329466929912 35.01588721618953 44-45 24.289702615992105 16.57514807502468 25.143447680157948 33.99170162882527 46-47 24.63521100691017 18.215541707798618 24.696908933859824 32.45233835143139 48-49 24.960667571569594 18.42068731490622 23.334927196446202 33.283717917077986 50-51 24.22029244817374 19.944626110562684 21.915874876604146 33.919206564659426 52-53 23.638018262586378 19.611457305034552 22.370125863770976 34.38039856860809 54-55 24.136229022704836 18.32042818361303 22.60072186574531 34.94262092793682 56-57 24.089955577492596 19.105534304047385 22.14801332675222 34.656496791707795 58-59 24.892028627838105 19.130984698914116 21.16084649555775 34.81614017769003 60-61 24.147026159921026 17.994200394866734 21.86574531095755 35.993028134254686 62-63 24.55885982230997 18.46773198420533 21.834125123395854 35.139283070088844 64-65 25.063240375123396 18.628146594274433 20.499136229022703 35.809476801579464 66-67 24.55885982230997 20.18524802566634 18.63277393879566 36.62311821322803 68-69 24.640609575518262 20.73281712734452 19.221217917077986 35.40535538005923 70-71 25.161185834155976 21.386815153010858 19.929972852912144 33.522026159921026 72-73 25.176610315893388 20.62176085883514 19.376233958538993 34.82539486673248 74-75 24.471711500493583 20.9333353899309 19.699376850937806 34.89557625863771 76 24.54652023692004 21.5541707798618 18.177751727541956 35.72155725567621 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 2.0 18 3.5 19 5.0 20 6.5 21 8.0 22 15.0 23 19.0 24 33.0 25 50.0 26 58.5 27 87.0 28 120.0 29 133.0 30 173.0 31 247.0 32 321.0 33 361.0 34 411.0 35 528.5 36 641.5 37 687.0 38 769.5 39 946.5 40 1150.5 41 1303.5 42 1347.0 43 1395.5 44 1459.5 45 1506.5 46 1538.0 47 1543.5 48 1484.5 49 1397.0 50 1374.0 51 1309.5 52 1180.5 53 1093.0 54 1070.0 55 1107.5 56 1168.5 57 1294.5 58 1397.0 59 1582.0 60 1870.0 61 2136.0 62 2299.0 63 2348.0 64 2607.5 65 2933.0 66 3163.5 67 3279.0 68 3431.0 69 3555.5 70 3438.0 71 3348.0 72 3279.5 73 3032.5 74 2604.5 75 2355.0 76 2122.5 77 1595.0 78 1070.0 79 840.0 80 652.5 81 333.5 82 148.0 83 94.0 84 63.5 85 24.0 86 9.5 87 4.0 88 4.0 89 2.5 90 1.0 91 3.0 92 5.0 93 4.0 94 5.0 95 4.0 96 1.0 97 1.5 98 3.0 99 12.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001542448173741362 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.71224086870681E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64832.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.32718410661401 #Duplication Level Percentage of deduplicated Percentage of total 1 85.93221269045746 72.4642152023692 2 10.7589032576686 18.145360315893384 3 2.480291196429551 6.274679170779862 4 0.6017815660953705 2.0298617966436328 5 0.14998811069854218 0.6324037512339585 6 0.05121545243364855 0.2591312931885489 7 0.012803863108412137 0.07557996051332676 8 0.003658246602403468 0.024679170779861793 9 0.003658246602403468 0.02776406712734452 >10 0.005487369903605202 0.06632527147087858 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004627344521224087 0.0 2 0.0 0.0 0.0 0.006169792694965449 0.0 3 0.0 0.0 0.0 0.006169792694965449 0.0 4 0.0 0.0 0.0 0.007712240868706812 0.0 5 0.0 0.0 0.0 0.01388203356367226 0.0 6 0.0 0.0 0.0 0.01388203356367226 0.0 7 0.0 0.0 0.0 0.01388203356367226 0.0 8 0.0 0.0 0.0 0.01388203356367226 0.0 9 0.0 0.0 0.0 0.018509378084896347 0.0 10 0.0 0.0 0.0 0.021594274432379074 0.0 11 0.0 0.0 0.0 0.023136722606120434 0.0 12 0.0 0.0 0.0 0.024679170779861797 0.0 13 0.0 0.0 0.0 0.033933859822309974 0.0 14 0.0 0.0 0.0 0.03547630799605133 0.0 15 0.0 0.0 0.0 0.037018756169792694 0.0 16 0.0 0.0 0.0 0.03856120434353406 0.0 17 0.0 0.0 0.0 0.03856120434353406 0.0 18 0.0 0.0 0.0 0.044730997038499504 0.0 19 0.0 0.0 0.0 0.05398568608094768 0.0 20 0.0 0.0 0.0 0.05861303060217177 0.0 21 0.0 0.0 0.0 0.061697926949654494 0.0 22 0.0 0.0 0.0 0.07249506416584403 0.0 23 0.0 0.0 0.0 0.0817497532082922 0.0 24 0.0 0.0 0.0 0.0863770977295163 0.0 25 0.0 0.0 0.0 0.10180157946692991 0.0 26 0.0 0.0 0.0 0.12031095755182626 0.0 27 0.0 0.0 0.0 0.20668805528134254 0.0 28 0.0 0.0 0.0 0.3069471865745311 0.0 29 0.0 0.0 0.0 0.4318854886475814 0.0 30 0.0 0.0 0.0 0.5938425468904245 0.0 31 0.0 0.0 0.0 0.7496298124383021 0.0 32 0.0 0.0 0.0 0.9223840078973347 0.0 33 0.0 0.0 0.0 1.1444965449160909 0.0 34 0.0 0.0015424481737413623 0.0 1.4499012833168805 0.0 35 0.0 0.0015424481737413623 0.0 1.8879565646594274 0.0 36 0.0 0.0015424481737413623 0.0 2.413931391905232 0.0 37 0.0 0.0015424481737413623 0.0 3.1234575518262586 0.0 38 0.0 0.0015424481737413623 0.0 3.8576628825271473 0.0 39 0.0 0.0015424481737413623 0.0 4.533255182625863 0.0 40 0.0 0.0015424481737413623 0.0 5.11784304047384 0.0 41 0.0 0.0015424481737413623 0.0 5.711685587364265 0.0 42 0.0 0.0015424481737413623 0.0 6.2762216189536035 0.0 43 0.0 0.0015424481737413623 0.0 6.853097235932872 0.0 44 0.0 0.0015424481737413623 0.0 7.43922754195459 0.0 45 0.0 0.0015424481737413623 0.0 8.124074531095756 0.0 46 0.0 0.0015424481737413623 0.0 8.750308489634747 0.0 47 0.0 0.0015424481737413623 0.0 9.304047384007898 0.0 48 0.0 0.0015424481737413623 0.0 9.777578973346495 0.0 49 0.0 0.0015424481737413623 0.0 10.215634254689043 0.0 50 0.0 0.0015424481737413623 0.0 10.74469397828233 0.0 51 0.0 0.0030848963474827246 0.0 11.201258637709772 0.0 52 0.0 0.0030848963474827246 0.0 11.750370187561698 0.0 53 0.0 0.0030848963474827246 0.0 12.265547877591313 0.0 54 0.0 0.0030848963474827246 0.0 12.69434846989141 0.0 55 0.0 0.0030848963474827246 0.0 13.147828232971372 0.0 56 0.0 0.0030848963474827246 0.0 13.607477788746298 0.0 57 0.0 0.0030848963474827246 0.0 14.090264067127345 0.0 58 0.0 0.0030848963474827246 0.0 14.542201382033564 0.0 59 0.0 0.0030848963474827246 0.0 14.977171767028628 0.0 60 0.0 0.0030848963474827246 0.0 15.442991115498518 0.0 61 0.0 0.0030848963474827246 0.0 15.902640671273446 0.0 62 0.0 0.0030848963474827246 0.0 16.336068608094767 0.0 63 0.0 0.0030848963474827246 0.0 16.80034550839092 0.0 64 0.0 0.0030848963474827246 0.0 17.209094274432378 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTGTGT 15 0.0022154166 70.0 33 AGTACTA 15 0.0022154166 70.0 21 ATATGAC 25 2.3741802E-4 56.000004 10 TTGGAGC 20 0.0069226176 52.5 68 GTGTGTA 20 0.0069226176 52.5 34 ACGTGAA 20 0.0069226176 52.5 9 CATATGA 35 0.001247022 40.0 9 CGAACGA 40 0.002402682 35.0 25 TCATTAA 40 0.002402682 35.0 60 CATTAAA 40 0.002402682 35.0 61 ACGAACG 40 0.002402682 35.0 24 ACGACTG 45 0.0042788135 31.111109 32 TATCATT 50 0.007161051 28.000002 58 TGACCGC 50 0.007161051 28.000002 14 CCGACGG 50 0.007161051 28.000002 23 GATCTCT 50 0.007161051 28.000002 41 GACGAAC 65 7.878138E-4 26.923077 23 TAGATCT 70 0.0012141048 25.0 39 GCGCCAC 75 0.0018145174 23.333334 6 CGACGAA 110 2.8689103E-5 22.272726 22 >>END_MODULE