FastQCFastQC Report
Wed 25 May 2016
SRR1780164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64832
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT673310.385303553800593TruSeq Adapter, Index 5 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG22133.413437808489635TruSeq Adapter, Index 5 (96% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG17952.7686944718657456TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC16422.532699901283317RNA PCR Primer, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5070.7820212240868707RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3270.5043805528134254TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT1950.30077739387956565No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1750.2699284304047384No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1490.229824777887463TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT1290.19897581441263573No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT1230.18972112537018757No Hit
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG1150.17738153998025666TruSeq Adapter, Index 5 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC820.1264807502467917TruSeq Adapter, Index 5 (95% over 24bp)
CAGCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGC800.12339585389930897No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG710.10951382033563672No Hit
ACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCT700.10797137216189535RNA PCR Primer, Index 5 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT700.10797137216189535RNA PCR Primer, Index 5 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATC207.872383E-570.035
GTGATAT207.872383E-570.034
ATATCGT207.872383E-570.037
TCTTTAT401.3096724E-1070.01
GTGGTTC150.002215416670.011
CTTAATA453.765308E-1062.2222182
TAATACA453.765308E-1062.2222184
TTAATAC453.765308E-1062.2222183
TCTTAAT453.765308E-1062.2222181
ATCGTAT308.29157E-658.33333239
AGTGATA252.3741802E-456.00000433
GATATCG252.3741802E-456.00000436
TATCGTA252.3741802E-456.00000438
AAACTCA200.006922617652.570
TATATAC200.006922617652.53
TTATATA200.006922617652.52
AAAAATG200.006922617652.568
CTTATAT200.006922617652.51
ATATACA200.006922617652.54
GGCTGAC200.006922617652.57
Produced by FastQC (version 0.11.5)