##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64832 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.69174173247779 34.0 34.0 34.0 34.0 34.0 2 33.674173247778874 34.0 34.0 34.0 34.0 34.0 3 33.680713228035536 34.0 34.0 34.0 34.0 34.0 4 33.71518694471866 34.0 34.0 34.0 34.0 34.0 5 33.693916584402764 34.0 34.0 34.0 34.0 34.0 6 37.403967176702864 38.0 38.0 38.0 37.0 38.0 7 37.38288191016782 38.0 38.0 38.0 37.0 38.0 8 37.28609328726555 38.0 38.0 38.0 36.0 38.0 9 37.314906219151034 38.0 38.0 38.0 36.0 38.0 10-11 37.245580885982235 38.0 38.0 38.0 36.0 38.0 12-13 37.22458045409674 38.0 38.0 38.0 36.0 38.0 14-15 37.13964554540967 38.0 38.0 38.0 36.0 38.0 16-17 37.161154985192496 38.0 38.0 38.0 36.0 38.0 18-19 37.05105503455084 38.0 38.0 38.0 35.5 38.0 20-21 37.13507218657453 38.0 38.0 38.0 36.0 38.0 22-23 37.11675561451135 38.0 38.0 38.0 36.0 38.0 24-25 37.10524895113524 38.0 38.0 38.0 35.5 38.0 26-27 36.9428985686081 38.0 38.0 38.0 35.0 38.0 28-29 36.942474395360314 38.0 38.0 38.0 35.0 38.0 30-31 36.92421952122409 38.0 38.0 38.0 35.0 38.0 32-33 36.654190831688055 38.0 37.0 38.0 34.0 38.0 34-35 36.783046952122405 38.0 37.0 38.0 34.5 38.0 36-37 36.813487166831194 38.0 37.0 38.0 34.5 38.0 38-39 35.59846217917078 38.0 36.5 38.0 31.0 38.0 40-41 36.1703711130306 38.0 37.0 38.0 33.5 38.0 42-43 36.6963228035538 38.0 37.0 38.0 34.0 38.0 44-45 36.82300407206318 38.0 37.0 38.0 34.5 38.0 46-47 36.76186913869694 38.0 37.0 38.0 34.5 38.0 48-49 36.785838783316876 38.0 37.0 38.0 34.5 38.0 50-51 36.525334711253706 38.0 37.0 38.0 34.0 38.0 52-53 36.506987290227045 38.0 37.0 38.0 34.0 38.0 54-55 36.483017645607106 38.0 37.0 38.0 34.0 38.0 56-57 36.55258205824284 38.0 37.0 38.0 34.0 38.0 58-59 36.33680898321816 38.0 37.0 38.0 34.0 38.0 60-61 36.36647026159921 38.0 37.0 38.0 34.0 38.0 62-63 36.35969120187562 38.0 37.0 38.0 34.0 38.0 64-65 36.17495989634748 38.0 37.0 38.0 32.5 38.0 66-67 35.527270483711746 38.0 36.5 38.0 29.5 38.0 68-69 35.349888943731486 38.0 36.0 38.0 29.0 38.0 70-71 34.247832860315896 38.0 35.0 38.0 24.0 38.0 72-73 32.36497408687068 38.0 29.5 38.0 11.0 38.0 74-75 31.796697618460023 38.0 27.0 38.0 11.0 38.0 76 31.01650419545903 37.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14134962629103853 1101 2 0.16298428921098917 1101 3 0.020865168015710367 1101 4 0.08720769055689459 1101 5 0.06852951087787318 1101 6 -0.03858248643869899 1101 7 0.16352260321633594 1101 8 -0.11589569607131267 1101 9 -0.06071657967070365 1101 10-11 0.3033174616846708 1101 12-13 0.3491260560154643 1101 14-15 0.35766866025515753 1101 16-17 -0.024214703207817934 1101 18-19 0.15769902090151788 1101 20-21 -0.10469456761592255 1101 22-23 0.05378813544763261 1101 24-25 0.11398788964158513 1101 26-27 -0.11339724361875625 1101 28-29 -0.15917062506962765 1101 30-31 0.2523505233430541 1101 32-33 -0.35077802637162847 1101 34-35 0.12511004216887756 1101 36-37 0.3336564893668452 1101 38-39 0.3622144951395043 1101 40-41 0.12945820211665193 1101 42-43 0.150272363511867 1101 44-45 0.23727442535127352 1101 46-47 0.25430838687007196 1101 48-49 0.432344713432407 1101 50-51 -0.17914768661979963 1101 52-53 0.08278013678707197 1101 54-55 0.3119060712414381 1101 56-57 0.134403054384876 1101 58-59 0.3081475493317569 1101 60-61 0.2651683714323312 1101 62-63 -0.08888413684225327 1101 64-65 -0.1749373574387576 1101 66-67 0.028395682573993497 1101 68-69 -0.43632504088998303 1101 70-71 -0.3159575520132165 1101 72-73 -1.096985182310931 1101 74-75 -0.6419107714862164 1101 76 -1.466682391330906 1102 1 0.06841589990169439 1102 2 0.11317535810497503 1102 3 -0.08801134688766865 1102 4 0.21471069306808488 1102 5 -0.11055117478649379 1102 6 0.15362190268675135 1102 7 0.03748264252193678 1102 8 0.0775315339756375 1102 9 -0.23955706443978642 1102 10-11 -0.27842727027296377 1102 12-13 -0.032662348832232624 1102 14-15 0.10461701706267235 1102 16-17 -0.08555327728795703 1102 18-19 -0.07869141473555175 1102 20-21 -0.05590103120364631 1102 22-23 0.05210672918057213 1102 24-25 -0.12518669273640626 1102 26-27 0.15335676882801863 1102 28-29 0.24066560159267425 1102 30-31 0.20213761800404484 1102 32-33 0.24262736723029832 1102 34-35 0.10070781485148217 1102 36-37 0.014265726183090521 1102 38-39 0.10372557713534292 1102 40-41 0.19182308911304347 1102 42-43 0.09589961539525405 1102 44-45 0.10228152219590925 1102 46-47 -0.14575712246501382 1102 48-49 -0.013522739346285562 1102 50-51 -0.06805477254620484 1102 52-53 -0.24543255018749477 1102 54-55 0.004678207937587331 1102 56-57 0.0931212550618028 1102 58-59 0.4160305059864058 1102 60-61 -0.11069143859059238 1102 62-63 0.1853925309162321 1102 64-65 0.18122597917004413 1102 66-67 0.2538678956976952 1102 68-69 -0.08111158963984622 1102 70-71 0.01264819097182368 1102 72-73 -0.816932774842865 1102 74-75 -0.7621968289160677 1102 76 -0.705311062583224 1103 1 -0.08084414238160775 1103 2 -0.0642030774129978 1103 3 0.03936708863030702 1103 4 -0.16985590312641818 1103 5 0.1491739837758672 1103 6 0.09611661727448961 1103 7 0.28695410129571997 1103 8 0.332076988521095 1103 9 0.13665858038051226 1103 10-11 0.22257695805684108 1103 12-13 0.08869071670687845 1103 14-15 0.07279883524449104 1103 16-17 0.23188858317715955 1103 18-19 0.1502726444610616 1103 20-21 0.12015605124878448 1103 22-23 0.0321278708296191 1103 24-25 0.26181119309868706 1103 26-27 0.18993182168213707 1103 28-29 0.0891328320366469 1103 30-31 0.045372290308478114 1103 32-33 0.3584836040167687 1103 34-35 -0.15494757626902356 1103 36-37 0.07642217438605314 1103 38-39 0.4996558096934862 1103 40-41 0.32004719059295894 1103 42-43 -0.06525260447789094 1103 44-45 0.2050827906948598 1103 46-47 0.381357042439852 1103 48-49 0.165229903359851 1103 50-51 0.17697693992736419 1103 52-53 0.2885526506581684 1103 54-55 0.05991076607711676 1103 56-57 0.2852459907911893 1103 58-59 0.44192902607097295 1103 60-61 0.26705697578572796 1103 62-63 -0.18368780735015378 1103 64-65 0.48307587346180014 1103 66-67 0.3507495024630174 1103 68-69 1.1673027866815104 1103 70-71 0.9796143643333437 1103 72-73 1.240466802324157 1103 74-75 0.5000653275321341 1103 76 0.6125536308628625 1104 1 0.04536865827065384 1104 2 -0.0053771499771997355 1104 3 -0.037620273783069536 1104 4 -0.024605189714272058 1104 5 -0.06381011732482023 1104 6 0.14805302167725642 1104 7 -0.013575862014512552 1104 8 -0.12964335391674098 1104 9 -0.21880411835572744 1104 10-11 0.09311303632835433 1104 12-13 0.08489944071828859 1104 14-15 0.06156768970841142 1104 16-17 -0.008245945970827506 1104 18-19 0.08989759350304638 1104 20-21 0.01168682988718217 1104 22-23 0.17796969716471978 1104 24-25 0.03276512705710388 1104 26-27 0.04247972221210716 1104 28-29 -0.034399577258092506 1104 30-31 0.06858875993751212 1104 32-33 -0.05969821416505283 1104 34-35 0.10164639030251976 1104 36-37 0.09892523186668711 1104 38-39 -0.7672724414275862 1104 40-41 -0.0855989569814426 1104 42-43 0.22672660465378414 1104 44-45 0.14706199982653345 1104 46-47 0.0051136669812308355 1104 48-49 0.08118995227954429 1104 50-51 0.10975313234481376 1104 52-53 -0.3133124125297613 1104 54-55 -0.07047039984216497 1104 56-57 -0.546663915446068 1104 58-59 0.05691068118716203 1104 60-61 -0.18348317912662537 1104 62-63 0.29159755750920624 1104 64-65 -0.0723562504680828 1104 66-67 -0.4138346801704884 1104 68-69 -0.22621535437026807 1104 70-71 -0.16909130219742963 1104 72-73 -1.2536832180590523 1104 74-75 -1.1365632904866168 1104 76 -0.904017914184017 1105 1 -0.2133116748491375 1105 2 -0.3581424713523873 1105 3 -0.02145542219220431 1105 4 0.1033042700337532 1105 5 0.20307008767197487 1105 6 0.19005601121388338 1105 7 0.03327444761606557 1105 8 -0.18913513269102822 1105 9 -0.08130678758485743 1105 10-11 0.22766353814342466 1105 12-13 0.11877729679346061 1105 14-15 0.1189027313483848 1105 16-17 0.2628409641295377 1105 18-19 0.16477480896322305 1105 20-21 0.23584869194142044 1105 22-23 0.09446553316728767 1105 24-25 -0.04966066837317129 1105 26-27 0.3174209991713184 1105 28-29 0.2303665982704146 1105 30-31 -0.312203467267274 1105 32-33 0.32125416678732677 1105 34-35 0.3018706055491549 1105 36-37 0.29438754235142284 1105 38-39 0.5175129525506321 1105 40-41 0.07177879232456519 1105 42-43 0.2004400362147507 1105 44-45 0.01839447900654534 1105 46-47 -0.0860671999843845 1105 48-49 -0.21745407932412775 1105 50-51 -0.2795165665661443 1105 52-53 0.20705914416466698 1105 54-55 0.07062505179140999 1105 56-57 0.21803819858340034 1105 58-59 0.22223205637400412 1105 60-61 0.41976260349135686 1105 62-63 0.3649052662429142 1105 64-65 -0.480235814849884 1105 66-67 -0.4642937875802673 1105 68-69 0.153882873261594 1105 70-71 -0.2717925620735855 1105 72-73 -0.32706566520831615 1105 74-75 0.15352853099533803 1105 76 0.17597354428277612 1106 1 0.08572740266527745 1106 2 0.0977618634757107 1106 3 0.07448734953531755 1106 4 0.12337687306600031 1106 5 0.0752033355899755 1106 6 0.19738037593734958 1106 7 -0.13016779026563086 1106 8 0.1932266012402124 1106 9 0.2744694242451686 1106 10-11 0.2814538435032361 1106 12-13 0.1633747770411702 1106 14-15 0.036904012578368395 1106 16-17 -0.0015194885717164652 1106 18-19 0.30962853520708933 1106 20-21 0.18433256979749046 1106 22-23 0.1622746299001463 1106 24-25 0.2255902391647311 1106 26-27 0.15010304059775592 1106 28-29 0.3355555796925813 1106 30-31 0.2793510917760713 1106 32-33 0.5075663598259794 1106 34-35 0.25187060554915774 1106 36-37 0.37470998522991295 1106 38-39 0.07801903839304458 1106 40-41 0.3987059757539839 1106 42-43 0.4330046315596121 1106 44-45 0.24347455588033995 1106 46-47 0.3212571647390803 1106 48-49 0.4354500419656375 1106 50-51 0.5649100830174518 1106 52-53 0.34363826011598064 1106 54-55 0.31966412930582067 1106 56-57 0.2134257706525844 1106 58-59 0.33045328208402935 1106 60-61 0.32324327827247856 1106 62-63 0.6727634767917152 1106 64-65 0.5307827629848347 1106 66-67 0.5323536606366872 1106 68-69 0.6302868877821979 1106 70-71 0.983375065515574 1106 72-73 0.4436261989813062 1106 74-75 0.1706115974057596 1106 76 0.3605560768473737 1107 1 0.10666124322804649 1107 2 0.1663441005275459 1107 3 0.14128436566606695 1107 4 0.09946766430141452 1107 5 0.08986765029892041 1107 6 0.2205457426514812 1107 7 0.10237848733621746 1107 8 0.22255523065810223 1107 9 0.2873910232930541 1107 10-11 0.33447915538558703 1107 12-13 0.2452611604811139 1107 14-15 0.2932426094797336 1107 16-17 0.20890280118663895 1107 18-19 -0.013751483070173265 1107 20-21 0.43326462513934416 1107 22-23 0.2308568669519886 1107 24-25 0.13494777215943543 1107 26-27 0.16455482900656193 1107 28-29 0.31492990226499984 1107 30-31 0.33432101930460334 1107 32-33 0.3123397005268913 1107 34-35 0.17841088990934395 1107 36-37 0.09749067934080813 1107 38-39 0.033186615380692785 1107 40-41 0.6180437438027866 1107 42-43 0.5563867750998739 1107 44-45 0.44117714657593865 1107 46-47 0.44868793429681375 1107 48-49 0.3937736345102181 1107 50-51 0.4327718559118523 1107 52-53 0.39825751924929165 1107 54-55 0.4164273537548411 1107 56-57 0.1598797693349212 1107 58-59 0.7337644418400444 1107 60-61 0.44436201671636155 1107 62-63 0.5510709173374835 1107 64-65 0.7678345981494417 1107 66-67 0.6209239962157156 1107 68-69 0.6282678879309671 1107 70-71 0.10513138285983814 1107 72-73 -0.2946689150390469 1107 74-75 -0.3179226087022471 1107 76 -0.5031508068794892 1108 1 0.09895383741637431 1108 2 0.1317565185466023 1108 3 0.04896304822626263 1108 4 -0.19106802433854142 1108 5 -0.15739167278978528 1108 6 -0.15438843323056517 1108 7 0.17483288917451034 1108 8 0.14722850367260776 1108 9 -0.07394748022554865 1108 10-11 -0.12313011265022311 1108 12-13 -0.3900971620809983 1108 14-15 -0.03982742738177336 1108 16-17 -0.06508111379253734 1108 18-19 -0.12043442624601397 1108 20-21 0.044651000743726854 1108 22-23 0.06495615365790997 1108 24-25 0.08706371835121018 1108 26-27 0.027305559055875506 1108 28-29 -0.2828368649330528 1108 30-31 -0.04957720464101101 1108 32-33 -0.14555680002364113 1108 34-35 -0.556210202531652 1108 36-37 0.13298783095170563 1108 38-39 -0.41524318020550055 1108 40-41 0.05338052392629322 1108 42-43 0.12338375915847166 1108 44-45 0.20281006342212748 1108 46-47 -0.08101669493387931 1108 48-49 -0.07668928855933643 1108 50-51 -0.30736649441607256 1108 52-53 -0.16473017762465503 1108 54-55 -0.03832155715520713 1108 56-57 -0.0508651203199193 1108 58-59 -0.028778044636091238 1108 60-61 0.014279198007947969 1108 62-63 -0.5410110396733856 1108 64-65 -0.018691803305877386 1108 66-67 0.00655758327110334 1108 68-69 -0.23572751634879552 1108 70-71 0.09754365726263359 1108 72-73 0.07430518616254034 1108 74-75 -0.22417709671028518 1108 76 0.2696243379335712 1109 1 -0.16402596056342844 1109 2 -0.07147580468572556 1109 3 -0.028360184096968055 1109 4 -0.206219539490057 1109 5 0.027355801957689607 1109 6 -0.3766106554527866 1109 7 -0.020773171431549997 1109 8 -0.07246846602436108 1109 9 0.2839313076532406 1109 10-11 -0.12674122376133568 1109 12-13 0.09937253488870113 1109 14-15 -0.0778829829373322 1109 16-17 -0.11061141682284159 1109 18-19 0.17072718991560265 1109 20-21 0.1281105967033227 1109 22-23 -0.10196303826128883 1109 24-25 0.007720284007781686 1109 26-27 -0.282340905590587 1109 28-29 -0.17927625887244147 1109 30-31 0.04946319939939059 1109 32-33 0.08780178583494802 1109 34-35 -0.04562939445084169 1109 36-37 0.0441494471133268 1109 38-39 -0.33784419030650525 1109 40-41 -0.11881644577067618 1109 42-43 -0.03479805902335187 1109 44-45 0.08553733614940029 1109 46-47 0.3097661333489441 1109 48-49 -0.35590646027651474 1109 50-51 -0.4466594237090007 1109 52-53 0.05241628702180634 1109 54-55 -0.14508923392288153 1109 56-57 -0.07339037284516792 1109 58-59 0.10056538970734152 1109 60-61 -0.01726120603245107 1109 62-63 -0.28414235280470024 1109 64-65 -0.04737867199273893 1109 66-67 0.0941585933721143 1109 68-69 0.13162096849968918 1109 70-71 0.4291598188788015 1109 72-73 -0.033851379494031164 1109 74-75 0.5648380548048664 1109 76 0.8757354490446829 1110 1 -0.01508813672922571 1110 2 -0.1267089653074862 1110 3 5.77639737230129E-4 1110 4 0.08134522734932403 1110 5 0.10492056879706979 1110 6 -0.03982309068594958 1110 7 -0.06971099526575131 1110 8 -0.07376380281192496 1110 9 0.29942353563251345 1110 10-11 -0.029578011326101716 1110 12-13 -0.09468186925637667 1110 14-15 -0.04875085858500228 1110 16-17 0.21349480079373961 1110 18-19 0.2781106096047239 1110 20-21 0.19890075214374292 1110 22-23 -0.015564074530715288 1110 24-25 0.04779800421503211 1110 26-27 0.005664275756558368 1110 28-29 -0.004496466126326482 1110 30-31 0.24435957245638917 1110 32-33 0.30558675992821094 1110 34-35 -0.5382848348653511 1110 36-37 0.20510799633612464 1110 38-39 0.32465580969348906 1110 40-41 0.025393398788907007 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-0.2740259605634279 2116 2 -0.19980913801905587 2116 3 0.09997314923636225 2116 4 -0.21455287282338986 2116 5 -0.09931086470897554 2116 6 -0.0016106554527866024 2116 7 0.10589349523511515 2116 8 0.2025315339756375 2116 9 -0.01940202568009397 2116 10-11 0.036592109571991216 2116 12-13 -0.25146079844464 2116 14-15 -0.04538298293733334 2116 16-17 -0.543111416822839 2116 18-19 -0.07093947675105738 2116 20-21 -0.09272273663000874 2116 22-23 -0.1936297049279574 2116 24-25 -0.27644638265888233 2116 26-27 0.245992427742749 2116 28-29 0.28155707446089195 2116 30-31 -0.07053680060060685 2116 32-33 -0.19719821416505567 2116 34-35 -0.3814627277841751 2116 36-37 -0.1675172195533463 2116 38-39 -1.167010856973178 2116 40-41 -0.47048311243734275 2116 42-43 -0.05313139235668274 2116 44-45 -0.9827959971839277 2116 46-47 0.13059946668227695 2116 48-49 -0.09423979360984447 2116 50-51 -0.20749275704233128 2116 52-53 -0.12091704631152567 2116 54-55 -0.027589233922881817 2116 56-57 -0.4450570395118376 2116 58-59 -0.3777679436259902 2116 60-61 -0.5105945393657905 2116 62-63 0.04585764719530516 2116 64-65 0.28345466134059194 2116 66-67 0.02915859337211657 2116 68-69 -0.817545698166974 2116 70-71 -1.0366735144545345 2116 72-73 -1.1996847128273593 2116 74-75 -0.9643286118618022 2116 76 -1.127597884288651 2117 1 0.14918832515085967 2117 2 -0.2093329475428689 2117 3 -0.1119316126683998 2117 4 0.1241376033670889 2117 5 -0.5219299123280265 2117 6 -0.0819677983099325 2117 7 0.07851254285416331 2117 8 -0.09925418031007638 2117 9 0.3032170219389556 2117 10-11 -0.04674122376133738 2117 12-13 -0.06366317939701105 2117 14-15 0.3153313027769542 2117 16-17 0.09171001174858873 2117 18-19 -0.022129952941533304 2117 20-21 -0.03796083186810506 2117 22-23 0.1408941045958514 2117 24-25 -0.025851144563652895 2117 26-27 -0.017698048447726933 2117 28-29 -0.39552625887244375 2117 30-31 0.1267846279708209 2117 32-33 0.17244464297780837 2117 34-35 0.14472774840629654 2117 36-37 0.11968516139903329 2117 38-39 0.23090580969348906 2117 40-41 0.1774335542293244 2117 42-43 0.34538051240522094 2117 44-45 0.23893019329226206 2117 46-47 0.2160161333489441 2117 48-49 0.09980782543777877 2117 50-51 0.20530486200528486 2117 52-53 0.3133091441646698 2117 54-55 0.2893750517914029 2117 56-57 -0.1292832299880331 2117 58-59 0.10199396113591064 2117 60-61 -0.14868977746102274 2117 62-63 -0.3452137813761311 2117 64-65 0.608157042292973 2117 66-67 1.2443371648006831 2117 68-69 1.450013825642543 2117 70-71 1.3841598188787998 2117 72-73 1.5086486205059728 2117 74-75 0.7309094833762941 2117 76 0.14859259190182428 2118 1 -0.11739330750219779 2118 2 -0.07923090672653643 2118 3 -0.22417651062757926 2118 4 -0.41030117214312156 2118 5 0.031641516243404055 2118 6 -0.6011004513711526 2118 7 -0.02863031428869789 2118 8 -0.27144805786109316 2118 9 -0.0513748147957358 2118 10-11 0.07698326603458128 2118 12-13 -0.06557644470314017 2118 14-15 0.10869864971572696 2118 16-17 0.0655620525649141 2118 18-19 0.07225780216050026 2118 20-21 -0.07367511758238976 2118 22-23 -0.22900385458781614 2118 24-25 -0.6636062466044663 2118 26-27 -0.3914225382436456 2118 28-29 -0.3298374833622404 2118 30-31 0.09425911776673956 2118 32-33 -0.5341880100834189 2118 34-35 -0.7022110271038997 2118 36-37 -0.6641158590091223 2118 38-39 -0.14983398622488409 2118 40-41 -0.05917358862781441 2118 42-43 -0.17949193657437235 2118 44-45 -0.1724218475240633 2118 46-47 0.07379674559383886 2118 48-49 -0.35044727660304886 2118 50-51 -0.4560471788110405 2118 52-53 -0.47559391705981824 2118 54-55 -0.041619846167776586 2118 56-57 -0.1605332299880331 2118 58-59 -0.23729175314979756 2118 60-61 -0.11935304276715186 2118 62-63 -0.4510811283149039 2118 64-65 -1.6832970393396849 2118 66-67 -0.8557903862197236 2118 68-69 -0.6488382151737824 2118 70-71 -0.7510442627538509 2118 72-73 0.4729343347916881 2118 74-75 0.6161135650089449 2118 76 0.03379667353447857 2119 1 0.10329962083192612 2119 2 -0.24961533956944493 2119 3 -0.3461508817713863 2119 4 -0.03180093483889124 2119 5 -0.3803186166469601 2119 6 -0.34172693452255487 2119 7 -0.37414526445481044 2119 8 -0.45037544276854646 2119 9 -0.35583613420722315 2119 10-11 -0.38540401445900585 2119 12-13 -0.7512669999950248 2119 14-15 -1.3163132154954695 2119 16-17 -0.37392537031121265 2119 18-19 -0.20776118217741413 2119 20-21 -0.016366147482720805 2119 22-23 0.2683857989480103 2119 24-25 -0.06239599506199056 2119 26-27 0.15568235022336552 2119 28-29 -0.07212509608174145 2119 30-31 -0.2850716843215366 2119 32-33 -1.082954028118536 2119 34-35 -0.07952474328804726 2119 36-37 -0.018292413351787218 2119 38-39 0.16767906550744271 2119 40-41 -0.212828073677656 2119 42-43 -0.7471236404186996 2119 44-45 -0.4256254545482747 2119 46-47 -0.863198982930129 2119 48-49 -0.277476227718374 2119 50-51 -0.7250315167322583 2119 52-53 -0.5024092943735425 2119 54-55 -0.24997295485311355 2119 56-57 -0.35571595424052305 2119 58-59 -0.24559740099033434 2119 60-61 0.3137271660605663 2119 62-63 -0.3773981667581907 2119 64-65 -1.0676112301322789 2119 66-67 -1.8205507089534692 2119 68-69 -1.7741348454538013 2119 70-71 -0.9850262276328294 2119 72-73 -0.4913513794940272 2119 74-75 -0.183127061474206 2119 76 -1.9332180393274108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 2.0 22 3.0 23 8.0 24 18.0 25 40.0 26 69.0 27 161.0 28 317.0 29 514.0 30 838.0 31 1339.0 32 2012.0 33 3497.0 34 5489.0 35 8710.0 36 10586.0 37 31226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.85112290227048 11.304602665350444 11.531342546890425 41.312931885488645 2 20.088845014807504 15.774617472852912 35.605873642645605 28.530663869693978 3 20.511475814412634 11.923124383020731 31.99808736426456 35.567312438302075 4 17.51758390918065 24.708477295162883 23.730565153010858 34.043373642645605 5 19.445644126357355 24.163993089832182 37.018756169792695 19.371606614017768 6 33.683983218163874 31.9286771964462 12.649617472852912 21.73772211253702 7 30.204220138203357 18.959772951628825 17.966436327739387 32.86957058242843 8 25.246791707798614 34.45057996051333 11.716436327739387 28.586192003948668 9 21.740807008884502 13.200271470878578 10.616670779861796 54.44225074037512 10-11 30.224271964461995 24.146254935834154 18.38906712734452 27.240405972359326 12-13 27.96998395853899 12.493830207305034 22.652393879565647 36.88379195459033 14-15 24.36682502467917 11.246760858835144 15.576412882527146 48.81000123395854 16-17 33.70249259624877 20.455947680157944 11.989449654491608 33.85211006910168 18-19 38.03600074037512 17.95486796643633 11.521316633761106 32.48781465942744 20-21 29.703695705824284 14.775882280355379 11.758082428430406 43.76233958538993 22-23 26.37355009871668 22.48580947680158 11.83289116485686 39.30774925962488 24-25 33.881416584402764 22.29531712734452 11.420286278381047 32.40298000987167 26-27 34.958045409674234 26.601061204343534 12.289455824284303 26.151437561697925 28-29 29.958199654491608 22.98787635735439 14.039363277393878 33.014560710760115 30-31 27.948389684106612 24.212580207305034 12.020298617966436 35.818731490621914 32-33 25.435741609081937 30.441757156959525 14.839893879565647 29.28260735439289 34-35 33.08936944718657 19.086253701875616 20.92330947680158 26.90106737413623 36-37 36.88379195459033 17.78596989141165 19.06620187561698 26.26403627838105 38-39 26.632681391905233 18.315029615004935 23.433643879565647 31.618645113524185 40-41 26.295656465942745 12.516966929911156 24.39690276406713 36.790473840078974 42-43 34.1436327739388 11.625431885488648 22.5089461994077 31.72198914116486 44-45 25.310803307008882 20.05182625863771 27.03448914116486 27.602881293188545 46-47 30.86130306021718 15.006478282329713 23.33107107601185 30.801147581441263 48-49 31.14819842053307 12.6025728035538 17.056391905231987 39.19283687068115 50-51 27.491825024679173 13.46248766041461 23.257033563672262 35.78865375123396 52-53 22.531311697926952 13.355287512339586 35.170132033563675 28.943268756169793 54-55 24.314381786771968 14.814443484698913 27.118552566633763 33.75262216189536 56-57 29.245588598223097 17.429664363277393 19.53433489634748 33.79041214215202 58-59 24.537265547877592 18.04355873642646 27.92910908193485 29.49006663376111 60-61 29.888789486673247 23.941109328726554 19.94076999012833 26.229331194471868 62-63 23.168342793682132 36.16423988153998 14.406465942744322 26.260951382033564 64-65 21.32203232971372 38.62675839091806 13.555034550839093 26.49617472852912 66-67 22.01073543928924 37.822371668311945 13.391535044422506 26.775357847976306 68-69 21.349796396841068 38.40464585389931 14.032422260612043 26.21313548864758 70-71 21.452369200394866 36.783532823297136 14.114943237907207 27.64915473840079 72-73 21.623580947680157 32.871113030602174 15.275635488647582 30.22967053307009 74-75 21.87654244817374 31.325579960513327 16.600598469891413 30.197279121421523 76 22.228220631786773 31.518385982230996 15.913437808489636 30.339955577492596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.5 23 3.0 24 5.5 25 8.0 26 8.5 27 13.0 28 19.5 29 22.0 30 37.5 31 50.0 32 77.5 33 108.0 34 122.5 35 151.5 36 185.0 37 204.0 38 351.0 39 2112.5 40 4508.5 41 4755.0 42 4220.0 43 3390.5 44 2006.5 45 1205.0 46 958.0 47 839.0 48 645.5 49 533.5 50 496.0 51 450.0 52 420.0 53 430.5 54 425.0 55 557.0 56 754.5 57 966.5 58 1113.0 59 1296.0 60 1612.5 61 1853.0 62 1960.0 63 2085.5 64 2394.5 65 2762.5 66 3116.0 67 3285.0 68 3359.0 69 3503.5 70 3508.0 71 3442.0 72 3339.0 73 3044.5 74 2596.5 75 2340.0 76 2100.5 77 1555.5 78 1009.0 79 768.0 80 588.5 81 303.0 82 136.0 83 75.0 84 48.0 85 13.5 86 4.0 87 2.0 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64832.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.476739881539984 #Duplication Level Percentage of deduplicated Percentage of total 1 74.09258500614501 41.84507650542942 2 19.093267786426328 21.56651036525173 3 4.872320087395876 8.255182625863771 4 1.245391233101188 2.813425468904245 5 0.3878191997815103 1.0951382033563672 6 0.07647139150621331 0.2591312931885489 7 0.04369793800355046 0.1727541954590326 8 0.02458009012699713 0.11105626850937808 9 0.019117847876553327 0.09717423494570583 >10 0.08466475488187901 1.0164733464955578 >50 0.027311211252219036 1.0149308983218164 >100 0.019117847876553327 1.8709896347482724 >500 0.0027311211252219036 0.7820212240868707 >1k 0.00819336337566571 8.714832181638696 >5k 0.0027311211252219036 10.385303553800593 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 6733 10.385303553800593 TruSeq Adapter, Index 5 (95% over 23bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 2213 3.413437808489635 TruSeq Adapter, Index 5 (96% over 29bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 1795 2.7686944718657456 TruSeq Adapter, Index 5 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 1642 2.532699901283317 RNA PCR Primer, Index 5 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 507 0.7820212240868707 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 327 0.5043805528134254 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 195 0.30077739387956565 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 175 0.2699284304047384 No Hit CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 149 0.229824777887463 TruSeq Adapter, Index 5 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT 129 0.19897581441263573 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT 123 0.18972112537018757 No Hit CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 115 0.17738153998025666 TruSeq Adapter, Index 5 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC 82 0.1264807502467917 TruSeq Adapter, Index 5 (95% over 24bp) CAGCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGC 80 0.12339585389930897 No Hit TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCG 71 0.10951382033563672 No Hit ACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCT 70 0.10797137216189535 RNA PCR Primer, Index 5 (96% over 28bp) TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT 70 0.10797137216189535 RNA PCR Primer, Index 5 (96% over 25bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004627344521224087 0.0 2 0.0 0.0 0.0 0.006169792694965449 0.0 3 0.0 0.0 0.0 0.006169792694965449 0.0 4 0.0 0.0 0.0 0.007712240868706812 0.0 5 0.0 0.0 0.0 0.015424481737413624 0.0 6 0.0 0.0 0.0 0.015424481737413624 0.0 7 0.0 0.0 0.0 0.015424481737413624 0.0 8 0.0 0.0 0.0 0.015424481737413624 0.0 9 0.0 0.0 0.0 0.02005182625863771 0.0 10 0.0 0.0 0.0 0.023136722606120434 0.0 11 0.0 0.0 0.0 0.024679170779861797 0.0 12 0.0 0.0 0.0 0.02622161895360316 0.0 13 0.0 0.0 0.0 0.03547630799605133 0.0 14 0.0 0.0 0.0 0.037018756169792694 0.0 15 0.0 0.0 0.0 0.03856120434353406 0.0 16 0.0 0.0 0.0 0.04010365251727542 0.0 17 0.0 0.0 0.0 0.04010365251727542 0.0 18 0.0 0.0 0.0 0.04627344521224087 0.0 19 0.0 0.0 0.0 0.06015547877591313 0.0 20 0.0 0.0 0.0 0.06632527147087858 0.0 21 0.0 0.0 0.0 0.07095261599210266 0.0 22 0.0 0.0 0.0 0.07712240868706811 0.0 23 0.0 0.0 0.0 0.08791954590325765 0.0 24 0.0 0.0 0.0 0.09100444225074038 0.0 25 0.0 0.0 0.0 0.106428923988154 0.0 26 0.0 0.0 0.0 0.12493830207305034 0.0 27 0.0 0.0 0.0 0.20668805528134254 0.0 28 0.0 0.0 0.0 0.31774432379072065 0.0 29 0.0 0.0 0.0 0.4442250740375123 0.0 30 0.0 0.0 0.0 0.6138943731490621 0.0 31 0.0 0.0 0.0 0.7743089832181639 0.0 32 0.0 0.0 0.0 0.9578603158933859 0.0 33 0.0 0.0 0.0 1.1768879565646595 0.0 34 0.0 0.0 0.0 1.4977171767028628 0.0 35 0.0 0.0 0.0 1.9589091806515302 0.0 36 0.0 0.0 0.0 2.503393385982231 0.0 37 0.0 0.0 0.0 3.2329713721618956 0.0 38 0.0 0.0 0.0 3.9887709772951627 0.0 39 0.0 0.0 0.0 4.696754689042448 0.0 40 0.0 0.0 0.0 5.2798000987166835 0.0 41 0.0 0.0 0.0 5.8813548864758145 0.0 42 0.0 0.0 0.0 6.422754195459032 0.0 43 0.0 0.0 0.0 6.9934600197433365 0.0 44 0.0 0.0 0.0 7.588845014807503 0.0 45 0.0 0.0 0.0 8.29991362290227 0.0 46 0.0 0.0 0.0 8.955454096742349 0.0 47 0.0 0.0 0.0 9.541584402764068 0.0 48 0.0 0.0 0.0 10.03208292201382 0.0 49 0.0 0.0 0.0 10.480935340572557 0.0 50 0.0 0.0 0.0 11.003825271470879 0.0 51 0.0 0.0 0.0 11.472729516288252 0.0 52 0.0 0.0 0.0 12.023383514313918 0.0 53 0.0 0.0 0.0 12.546273445212242 0.0 54 0.0 0.0 0.0 12.971989141164856 0.0 55 0.0 0.0 0.0 13.414671767028628 0.0 56 0.0 0.0 0.0 13.885118460019743 0.0 57 0.0 0.0 0.0 14.374074531095756 0.0 58 0.0 0.0 0.0 14.84452122408687 0.0 59 0.0 0.0 0.0 15.288746298124384 0.0 60 0.0 0.0 0.0 15.774617472852912 0.0 61 0.0 0.0 0.0 16.249691510365253 0.0 62 0.0 0.0 0.0 16.6877467917078 0.0 63 0.0 0.0 0.0 17.14431145113524 0.0 64 0.0 0.0 0.0 17.57311204343534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATATC 20 7.872383E-5 70.0 35 GTGATAT 20 7.872383E-5 70.0 34 ATATCGT 20 7.872383E-5 70.0 37 TCTTTAT 40 1.3096724E-10 70.0 1 GTGGTTC 15 0.0022154166 70.0 11 CTTAATA 45 3.765308E-10 62.222218 2 TAATACA 45 3.765308E-10 62.222218 4 TTAATAC 45 3.765308E-10 62.222218 3 TCTTAAT 45 3.765308E-10 62.222218 1 ATCGTAT 30 8.29157E-6 58.333332 39 AGTGATA 25 2.3741802E-4 56.000004 33 GATATCG 25 2.3741802E-4 56.000004 36 TATCGTA 25 2.3741802E-4 56.000004 38 AAACTCA 20 0.0069226176 52.5 70 TATATAC 20 0.0069226176 52.5 3 TTATATA 20 0.0069226176 52.5 2 AAAAATG 20 0.0069226176 52.5 68 CTTATAT 20 0.0069226176 52.5 1 ATATACA 20 0.0069226176 52.5 4 GGCTGAC 20 0.0069226176 52.5 7 >>END_MODULE