FastQCFastQC Report
Wed 25 May 2016
SRR1780163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290547
Sequences flagged as poor quality0
Sequence length50
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTG207.8915013E-443.9492262
GATCTCT301.3062308E-436.6306641
AAACTCG250.002355235235.18360527
CGAAACT353.2206762E-431.4139325
TTTACAC353.233715E-431.3923032
AAGTATG300.00575099829.31966827
TGATACA300.005770392329.2994842
GAAACTC407.038321E-427.4871926
CTTTACA407.066721E-427.4682661
TTACACA407.066721E-427.4682663
TAGATCT1351.10958354E-1021.20453339
CCAATGT550.00450107619.9803631
ACCCAAT708.1284565E-418.84835829
ATAAGAG708.166198E-418.8353823
TATAAGA708.166198E-418.8353822
CAATGTG600.0074409418.3153332
AATGTGT600.0074409418.3153333
TGTAGAT1751.8735591E-1017.61607637
CGACCAA750.001288715317.59786222
GACCCAA750.001291696617.591828
Produced by FastQC (version 0.11.5)