FastQCFastQC Report
Wed 25 May 2016
SRR1780163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290547
Sequences flagged as poor quality0
Sequence length50
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT181596.249935466551023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC47971.6510237586345753No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG47551.6365682660636662No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG28440.9788433540872905TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC9990.3438342161509153No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT7780.26777079095636847No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT5300.18241454910909424TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT408.105417E-944.1033481
GGTATAT207.756593E-444.1033481
TCTTTAT1150.040.2682721
TCCTTAT250.00232296235.282681
TCTTCTG3600.034.83101744
GTCTTCT3600.034.83101743
ATGCCGT24300.033.9483644
CTTTATA1500.030.8032532
CGTCTTC4150.030.74494442
TTTATAC1600.030.2531973
GTGTAGT300.00567249229.4022311
TCTATAC453.9945568E-529.402231
CTTAATA602.9296461E-729.3364322
TTAATAC602.9296461E-729.3364323
CCGTCTT4400.028.49810441
TATGCCG29350.028.18211443
AATACAC655.9085687E-727.075125
TAATACA701.1262837E-625.1455124
TCTGATA1355.456968E-1222.813333
ATCGTAT1355.456968E-1222.813339
Produced by FastQC (version 0.11.5)