FastQCFastQC Report
Wed 25 May 2016
SRR1780162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141798
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6812748.04510641899039No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG100457.084020931183797No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT9520.6713775934780463No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC5890.4153796245363123No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG5550.39140185334066774No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3490.2461247690376451No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG2670.18829602674226717No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1660.11706794171991142No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC301.0178155E-770.019
GCTGCGG301.0178155E-770.017
TGCGGGC150.002220871970.019
GCCGAAT150.002220871970.054
ACGTCTG150.002220871970.08
ACGTATC207.904701E-570.055
CCGAATC150.002220871970.055
CACGTCT150.002220871970.07
TTGGGCG150.002220871970.02
CAGCCGA352.9572584E-760.018
CTGCGGG352.9572584E-760.018
GGGCGTC352.9572584E-760.06
GCGCGTC308.342411E-658.3333326
GGCGTCT308.342411E-658.3333327
TGGGCGT308.342411E-658.3333325
GACGTAT252.3838678E-456.00000454
GTCTCGG252.3838678E-456.00000442
TGGGCGC252.3838678E-456.0000043
AAAAAGG6550.054.50381570
ATCTCGG14650.054.4709942
Produced by FastQC (version 0.11.5)