##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141798 Sequences flagged as poor quality 0 Sequence length 76 %GC 75 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.53560699022553 32.0 27.0 32.0 22.0 32.0 2 29.189283346732676 32.0 32.0 32.0 13.0 32.0 3 29.001128365703323 32.0 32.0 32.0 13.0 32.0 4 28.34543505550149 32.0 27.0 32.0 13.0 32.0 5 29.230320596905457 32.0 32.0 32.0 13.0 32.0 6 33.25169607469781 37.0 37.0 37.0 13.0 37.0 7 32.92130354447877 37.0 32.0 37.0 13.0 37.0 8 33.1729573054627 37.0 37.0 37.0 13.0 37.0 9 33.32594253797656 37.0 37.0 37.0 13.0 37.0 10-11 33.14513251244729 37.0 34.5 37.0 17.5 37.0 12-13 32.93275998251033 37.0 32.0 37.0 13.0 37.0 14-15 33.32518089112681 37.0 34.5 37.0 17.5 37.0 16-17 33.68514718120143 37.0 37.0 37.0 20.0 37.0 18-19 33.285049859659516 37.0 37.0 37.0 17.5 37.0 20-21 33.677604761703265 37.0 37.0 37.0 20.0 37.0 22-23 33.74098012665905 37.0 37.0 37.0 24.5 37.0 24-25 33.09914455775117 37.0 34.5 37.0 17.5 37.0 26-27 33.4769531305096 37.0 34.5 37.0 20.0 37.0 28-29 33.566806301922455 37.0 37.0 37.0 22.0 37.0 30-31 33.486822804270865 37.0 37.0 37.0 22.0 37.0 32-33 33.57423235870746 37.0 37.0 37.0 22.0 37.0 34-35 33.564408524802886 37.0 37.0 37.0 22.0 37.0 36-37 34.20382868587709 37.0 37.0 37.0 24.5 37.0 38-39 34.3166899392093 37.0 37.0 37.0 27.0 37.0 40-41 33.78467961466312 37.0 37.0 37.0 24.5 37.0 42-43 33.67952298339892 37.0 37.0 37.0 22.0 37.0 44-45 33.50862141920196 37.0 37.0 37.0 22.0 37.0 46-47 33.72399117053837 37.0 37.0 37.0 20.0 37.0 48-49 34.18441374349427 37.0 37.0 37.0 24.5 37.0 50-51 34.40916656088238 37.0 37.0 37.0 27.0 37.0 52-53 33.67122596933666 37.0 37.0 37.0 22.0 37.0 54-55 33.806062144741105 37.0 37.0 37.0 24.5 37.0 56-57 33.643672689318606 37.0 37.0 37.0 17.5 37.0 58-59 33.319584902466886 37.0 37.0 37.0 17.5 37.0 60-61 33.205602335717 37.0 37.0 37.0 17.5 37.0 62-63 32.79721152625566 37.0 34.5 37.0 13.0 37.0 64-65 32.55235969477708 37.0 34.5 37.0 13.0 37.0 66-67 32.81113626426325 37.0 32.0 37.0 13.0 37.0 68-69 33.0333220496763 37.0 32.0 37.0 13.0 37.0 70-71 33.05072709065008 37.0 32.0 37.0 13.0 37.0 72-73 33.25221089154995 37.0 37.0 37.0 17.5 37.0 74-75 32.71127237337621 37.0 34.5 37.0 13.0 37.0 76 32.520973497510546 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.1561915820794724 11101 2 -1.3356053848791838 11101 3 -1.1480575561477124 11101 4 -1.2157527396867494 11101 5 -1.2597323761612742 11101 6 -1.3632797354219583 11101 7 -1.467790697471301 11101 8 -2.1723587039696888 11101 9 -0.017305999334340072 11101 10-11 -0.7293187072166276 11101 12-13 -1.0407855141098992 11101 14-15 -0.3719090322552887 11101 16-17 -0.9593682242984158 11101 18-19 -0.5366250364314809 11101 20-21 -0.5261977514945642 11101 22-23 -0.15989712206537376 11101 24-25 -0.4166222081023676 11101 26-27 -0.7955322563246341 11101 28-29 -0.7700704433654053 11101 30-31 -0.42737770488324855 11101 32-33 0.029449374710459608 11101 34-35 -0.5910938574375564 11101 36-37 -0.25089507474564954 11101 38-39 -0.5469916437066047 11101 40-41 -0.5256902764679694 11101 42-43 -0.7782253518567117 11101 44-45 -1.524867341839638 11101 46-47 -0.937772216409904 11101 48-49 -0.8017047759779672 11101 50-51 -0.44745578512432616 11101 52-53 -1.5659313104124806 11101 54-55 -0.6902335594369688 11101 56-57 -1.1356460493237464 11101 58-59 -0.8027681918832599 11101 60-61 -0.3792935042946226 11101 62-63 -0.10216380087651089 11101 64-65 -0.45236417436462517 11101 66-67 0.4936448068192263 11101 68-69 -0.2355688828143414 11101 70-71 -0.0346060783446589 11101 72-73 -0.33910341401825406 11101 74-75 -0.6891982965541175 11101 76 0.5525121151468255 11102 1 -0.34455756392371484 11102 2 -1.0293464264463132 11102 3 0.04950575011124414 11102 4 -0.8145343928162276 11102 5 -1.2480266905425452 11102 6 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0.41395599787698956 21611 72-73 0.13798332902592847 21611 74-75 -0.6347793112840705 21611 76 0.06712516167475968 21612 1 1.0696936438057563 21612 2 0.584663235389435 21612 3 0.9833221752320185 21612 4 0.10744628351227448 21612 5 1.0838573674284682 21612 6 1.6013112291690064 21612 7 1.2151152854346847 21612 8 1.1825863509753631 21612 9 0.7077245256961788 21612 10-11 0.9245274466295257 21612 12-13 0.36782254449816065 21612 14-15 0.5213754610292085 21612 16-17 1.0864193214891316 21612 18-19 0.7489060990996563 21612 20-21 0.25853973324291957 21612 22-23 0.7850967729285117 21612 24-25 1.2847208932407312 21612 26-27 0.5774836166912394 21612 28-29 0.2660711927762307 21612 30-31 -0.8500272775328241 21612 32-33 0.18531018057127113 21612 34-35 0.1468182304745298 21612 36-37 0.6758448519942775 21612 38-39 0.6896994429844838 21612 40-41 -0.05670370748140385 21612 42-43 -0.2523401259714859 21612 44-45 0.7018115348392371 21612 46-47 0.36509713645944686 21612 48-49 0.9918239175507182 21612 50-51 0.574583286914752 21612 52-53 0.6405399960588269 21612 54-55 0.09883847963507009 21612 56-57 0.7808374671597775 21612 58-59 1.5635321744171122 21612 60-61 1.495737020735902 21612 62-63 0.32701812830541854 21612 64-65 0.6814575594571082 21612 66-67 -0.11459695142252713 21612 68-69 -0.2886824359278961 21612 70-71 0.3314500877115023 21612 72-73 0.5596145346996906 21612 74-75 0.3992632419074198 21612 76 -0.7229457603110525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 17.0 12 47.0 13 104.0 14 233.0 15 378.0 16 593.0 17 891.0 18 1162.0 19 1230.0 20 1256.0 21 1409.0 22 1499.0 23 1818.0 24 2053.0 25 2325.0 26 2624.0 27 2803.0 28 3106.0 29 3499.0 30 4095.0 31 4995.0 32 6080.0 33 8618.0 34 14568.0 35 25739.0 36 50654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.69630037095023 8.122822606806865 5.493730518060904 18.687146504182007 2 59.873199904088914 8.44511206081891 23.093414575663974 8.5882734594282 3 59.323121623718244 9.479682365054515 22.282401726399527 8.914794284827712 4 59.25962494622442 16.038168315784276 14.390696296714225 10.311510441277074 5 58.37176829010282 13.962115121510882 22.938969520021438 4.727147068364857 6 64.44182717059375 22.47290063684385 7.655526013272869 5.429746179289528 7 62.82669713254065 12.967037616891636 8.399272204121356 15.806993046446353 8 62.925429131581545 21.780278988420147 7.6002482404547305 7.694043639543575 9 62.002990169113815 7.8809292091566885 7.311104528977842 22.804976092751662 10-11 63.28826922805681 14.543223458722974 14.093287634522348 8.075219678697866 12-13 62.39298156532532 9.712055177082894 14.377494746047196 13.517468511544593 14-15 67.96393461120749 13.03861831619628 9.051961240638091 9.945485831958138 16-17 67.34333347437904 10.636609825244362 8.469089832014557 13.550966868362035 18-19 62.46231421569492 9.772386678185441 13.561240501419277 14.204058604700364 20-21 67.50202757502028 9.678408970697133 9.87975598575408 12.93980746852851 22-23 67.6462291428652 9.449004922495382 8.526565960027645 14.378199974611771 24-25 63.61110327512765 14.67566080848543 8.362718271319359 13.350517645067562 26-27 67.68103472594431 13.957728003610823 9.339473609974894 9.02176366046997 28-29 67.42972397353982 10.266364828841027 9.834412333037138 12.469498864582011 30-31 63.66950996329221 9.731267917529117 18.13774061941317 8.461481499765508 32-33 67.64531644498358 9.675656233691129 10.745165521813336 11.933861799511968 34-35 67.61555170030607 9.465577793762957 14.377494746047196 8.541375759883778 36-37 68.60392953356182 9.44618400823707 14.359511417650461 7.590375040550643 38-39 67.95758755412629 9.561488878545536 14.992806668641306 7.488116898686864 40-41 67.53973962961396 13.967051721462925 10.584422911465605 7.90878573745751 42-43 63.64046687118727 14.040339927359922 14.308332451778977 8.010860749673824 44-45 62.76428440457552 9.326295152258847 15.18251315251273 12.7269072906529 46-47 66.8591940647964 8.814651828657668 10.89084472277465 13.43530938377128 48-49 68.14623619515085 8.834398228465846 14.722704128408019 8.29666144797529 50-51 71.99290540064035 8.87424364236449 10.83724735186674 8.295603605128422 52-53 63.26464407114346 9.20464322486918 14.68744270017913 12.843270003808236 54-55 66.86448327903072 9.119663182837558 10.285053385802339 13.730800152329369 56-57 66.81217934025635 9.322449267441245 9.980782453851443 13.884588938450959 58-59 62.80065305316459 13.815671160227227 14.568515926104848 8.81515986050333 60-61 61.76766163014158 10.417320474620498 15.04081524709533 12.7742026481426 62-63 61.87534379892523 14.526650587455395 14.167689248085304 9.43031636553407 64-65 62.148657750069646 14.625993067480985 14.690522618841923 8.53482656360745 66-67 62.047103046288484 19.89273284413116 9.370889767305734 8.689274342274613 68-69 62.1683662675073 20.17200524690052 8.904215856359047 8.755412629233135 70-71 62.58608226520214 20.159382217599042 8.902836791903947 8.351698725294874 72-73 63.31234088168297 19.23877091898982 8.692003356911641 8.756884842415566 74-75 66.08519956416413 16.656617053312317 8.72623795368715 8.531945428836396 76 73.29562684683033 9.114438246225237 8.91133098725643 8.678603919688005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.5 8 3.0 9 3.5 10 2.0 11 0.5 12 1.0 13 4.5 14 7.5 15 8.5 16 12.0 17 14.0 18 25.0 19 36.0 20 50.0 21 64.0 22 79.0 23 123.5 24 171.5 25 190.0 26 227.0 27 332.5 28 466.5 29 532.0 30 583.5 31 720.5 32 899.0 33 992.0 34 1136.5 35 1358.0 36 1543.5 37 1652.0 38 1871.0 39 2267.0 40 2582.5 41 2832.5 42 2944.0 43 2987.0 44 3582.5 45 6862.0 46 9589.0 47 8932.0 48 5573.0 49 2697.0 50 2523.0 51 2265.5 52 1924.5 53 1656.5 54 1472.0 55 1365.5 56 1203.5 57 1070.5 58 993.0 59 859.5 60 678.0 61 589.5 62 549.0 63 516.0 64 470.5 65 415.0 66 327.5 67 283.0 68 304.5 69 307.0 70 279.5 71 271.0 72 265.5 73 246.5 74 221.5 75 210.0 76 201.0 77 187.5 78 168.0 79 153.0 80 162.5 81 179.0 82 177.5 83 169.0 84 198.5 85 216.0 86 217.0 87 230.0 88 254.5 89 297.0 90 360.5 91 423.0 92 440.0 93 483.5 94 588.0 95 785.0 96 921.0 97 1157.5 98 2115.5 99 36050.0 100 69263.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.003526142822888898 5 0.0 6 0.003526142822888898 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.526142822888898E-4 20-21 0.002115685693733339 22-23 0.0 24-25 0.0014104571291555592 26-27 0.0014104571291555592 28-29 0.0 30-31 0.001763071411444449 32-33 0.0028209142583111185 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.002115685693733339 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.526142822888898E-4 58-59 0.001763071411444449 60-61 3.526142822888898E-4 62-63 0.0 64-65 0.001763071411444449 66-67 0.001763071411444449 68-69 0.0 70-71 3.526142822888898E-4 72-73 7.052285645777796E-4 74-75 0.0010578428468666695 76 7.052285645777796E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 141798.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.242937135925754 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24365836630207 17.998843425154092 2 9.189550849429835 4.455634071002412 3 6.16418431463812 4.483137985020946 4 4.058063765417733 3.9351753903440105 5 2.5453804980218755 3.085374970027786 6 1.3963230160577147 2.0310582659840053 7 0.8145217593670002 1.3822479865724482 8 0.4538049802187573 0.8801252485930691 9 0.24726553409355365 0.5394998519020014 >10 0.8174307656504539 3.113584112610897 >50 0.0319990691179893 0.528216194868757 >100 0.023272050267628578 0.959816076390358 >500 0.008727018850360717 1.478159071355026 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0058180125669071445 55.12912735017419 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 68127 48.04510641899039 No Hit CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG 10045 7.084020931183797 No Hit TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT 952 0.6713775934780463 No Hit ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC 589 0.4153796245363123 No Hit CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 555 0.39140185334066774 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 349 0.2461247690376451 No Hit TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 267 0.18829602674226717 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 166 0.11706794171991142 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0035261428228888984 0.0 2 0.0 0.0 0.0 0.004231371387466678 0.0 3 0.0 0.0 0.0 0.005641828516622237 0.0 4 0.0 0.0 0.0 0.01692548554986671 0.0 5 0.0 0.0 0.0 0.02186208550191117 0.0 6 0.0 0.0 0.0 0.02186208550191117 0.0 7 0.0 0.0 0.0 0.02186208550191117 0.0 8 0.0 0.0 0.0 0.02186208550191117 0.0 9 0.0 0.0 0.0 0.02397777119564451 0.0 10 0.0 0.0 0.0 0.02468299976022229 0.0 11 0.0 0.0 0.0 0.026093456889377847 0.0 12 0.0 0.0 0.0 0.026093456889377847 0.0 13 0.0 0.0 0.0 0.026093456889377847 0.0 14 0.0 0.0 0.0 0.03173528540600008 0.0 15 0.0 0.0 0.0 0.03173528540600008 0.0 16 0.0 0.0 0.0 0.03173528540600008 0.0 17 0.0 0.0 0.0 0.032440513970577865 0.0 18 0.0 0.0 0.0 0.03314574253515564 0.0 19 0.0 0.0 0.0 0.03667188535804454 0.0 20 0.0 0.0 0.0 0.03949279961635566 0.0 21 0.0 0.0 0.0 0.04583985669755568 0.0 22 0.0 0.0 0.0 0.06347057081200017 0.0 23 0.0 0.0 0.0 0.07404899928066687 0.0 24 0.0 0.0 0.0 0.09026925626595579 0.0 25 0.0 0.0 0.0 0.09450062765342247 0.0 26 0.0 0.0 0.0 0.09520585621800025 0.0 27 0.0 0.0 0.0 0.11213134176786696 0.0 28 0.0 0.0 0.0 0.13751957009266702 0.0 29 0.0 0.0 0.0 0.1488032271259115 0.0 30 0.0 0.0 0.0 0.17419145545071157 0.0 31 0.0 0.0 0.0 0.18970648387142272 0.0 32 0.0 0.0 0.0 0.20945288367960055 0.0 33 0.0 0.0 0.0 0.24824045473137843 0.0 34 0.0 0.0 0.0 0.2799757401373785 0.0 35 0.0 0.0 0.0 0.35543519654720096 0.0 36 0.0 0.0 0.0 0.4541671955880901 0.0 37 0.0 0.0 0.0 0.6311795652971128 0.0 38 0.0 0.0 0.0 0.8533265631391134 0.0 39 0.0 0.0 0.0 1.104387932128803 0.0 40 0.0 0.0 0.0 1.3871845865244925 0.0 41 0.0 0.0 0.0 1.6897276407283601 0.0 42 0.0 0.0 0.0 2.1234432079436947 0.0 43 0.0 0.0 0.0 2.4908672900887177 0.0 44 0.0 0.0 0.0 2.8032835441966744 0.0 45 0.0 0.0 0.0 3.1037109127068083 0.0 46 0.0 0.0 0.0 3.4527990521728094 0.0 47 0.0 0.0 0.0 3.73206956374561 0.0 48 0.0 0.0 0.0 4.060706074838856 0.0 49 0.0 0.0 0.0 4.325166786555522 0.0 50 0.0 0.0 0.0 4.594564098224234 0.0 51 0.0 0.0 0.0 4.9309581235278355 0.0 52 0.0 0.0 0.0 5.2765201201709475 0.0 53 0.0 0.0 0.0 5.589641602843481 0.0 54 0.0 0.0 0.0 5.842818657526904 0.0 55 0.0 0.0 0.0 6.1594662830223275 0.0 56 0.0 0.0 0.0 6.418990394786951 0.0 57 0.0 0.0 0.0 6.619980535691617 0.0 58 0.0 0.0 0.0 6.868220990422996 0.0 59 0.0 0.0 0.0 7.06709544563393 0.0 60 0.0 0.0 0.0 7.321682957446509 0.0 61 0.0 0.0 0.0 7.56569204079042 0.0 62 0.0 0.0 0.0 7.785018124374109 0.0 63 0.0 0.0 0.0 8.036079493363799 0.0 64 0.0 0.0 0.0 8.251174205560021 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGAC 30 1.0178155E-7 70.0 19 GCTGCGG 30 1.0178155E-7 70.0 17 TGCGGGC 15 0.0022208719 70.0 19 GCCGAAT 15 0.0022208719 70.0 54 ACGTCTG 15 0.0022208719 70.0 8 ACGTATC 20 7.904701E-5 70.0 55 CCGAATC 15 0.0022208719 70.0 55 CACGTCT 15 0.0022208719 70.0 7 TTGGGCG 15 0.0022208719 70.0 2 CAGCCGA 35 2.9572584E-7 60.0 18 CTGCGGG 35 2.9572584E-7 60.0 18 GGGCGTC 35 2.9572584E-7 60.0 6 GCGCGTC 30 8.342411E-6 58.333332 6 GGCGTCT 30 8.342411E-6 58.333332 7 TGGGCGT 30 8.342411E-6 58.333332 5 GACGTAT 25 2.3838678E-4 56.000004 54 GTCTCGG 25 2.3838678E-4 56.000004 42 TGGGCGC 25 2.3838678E-4 56.000004 3 AAAAAGG 655 0.0 54.503815 70 ATCTCGG 1465 0.0 54.47099 42 >>END_MODULE