FastQCFastQC Report
Wed 25 May 2016
SRR1780162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141798
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2429517.133527976417156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1527610.773071552490162No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT97996.910534704297663No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG42753.014852113570008No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT25621.8067955824482715No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT23811.6791492122596934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC8850.624127279651335No Hit
CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT7360.5190482235292458No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT6440.4541671955880901No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC5470.3857600248240455No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT4880.3441515395139565No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT4610.32511036827035644No Hit
CAAAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT4050.28561756865400073No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT3950.278565283008223No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT3660.25811365463546737No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT3490.2461247690376451RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT3170.22355745497115614No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT3010.21227379793791168No Hit
TCTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2900.2045162837275561No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2770.19534831238804495No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2460.1734862268861338No Hit
CTAAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2350.1657287126757782No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC2350.1657287126757782No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT2170.1530345985133782No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2040.14386662717386706No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1890.13328819870520034No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1890.13328819870520034No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT1810.1276463701885781No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT1720.1212993131073781No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT1620.11424702746160031No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT1580.11142611320328918No Hit
CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT1530.10789997038040029No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG1510.10648951325124474No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT1460.10296337042835584No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1430.1008476847346225No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1420.1001424561700447No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA150.002220960369.999310
AGACTCG301.0178883E-769.999325
AGAAGCG150.002220960369.99935
GCGTTAT150.002220960369.999311
CGTTATG150.002220960369.999312
TCTACAG150.002220960369.999350
GAGCGTT150.002220960369.99939
TCGCTCT150.002220960369.999346
AGTCTTA150.002220960369.999348
ACGTCAA150.002220960369.999347
CTACAGC150.002220960369.999351
CTCGAAG301.0178883E-769.999328
AGCCTAT150.002220960369.999330
TATAACG150.002220960369.999343
ATACCGA150.002220960369.999344
AACACGT150.002220960369.999337
GTATTAA401.3460522E-1069.9992949
ACACCGA207.905096E-569.9992911
TTCCGTA207.905096E-569.9992945
TAACGTA207.905096E-569.9992938
Produced by FastQC (version 0.11.5)