##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780162_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 141798 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.028808586863 32.0 27.0 32.0 22.0 32.0 2 23.33868601813848 27.0 13.0 32.0 8.0 32.0 3 27.889751618499556 32.0 27.0 32.0 13.0 32.0 4 27.391345435055502 32.0 27.0 32.0 13.0 32.0 5 25.061658133401036 27.0 22.0 32.0 8.0 32.0 6 31.254538145813058 37.0 27.0 37.0 13.0 37.0 7 33.58389399004217 37.0 32.0 37.0 27.0 37.0 8 31.267189946261585 37.0 27.0 37.0 13.0 37.0 9 34.869137787556944 37.0 37.0 37.0 32.0 37.0 10-11 33.46100438652167 37.0 34.5 37.0 24.5 37.0 12-13 34.48747161455027 37.0 37.0 37.0 29.5 37.0 14-15 34.70438229030029 37.0 37.0 37.0 27.0 37.0 16-17 32.8333439117618 37.0 32.0 37.0 22.0 37.0 18-19 33.76037038604211 37.0 34.5 37.0 24.5 37.0 20-21 34.64913821069409 37.0 37.0 37.0 27.0 37.0 22-23 33.96143104980324 37.0 34.5 37.0 24.5 37.0 24-25 34.236068209706765 37.0 37.0 37.0 27.0 37.0 26-27 34.52709135530826 37.0 37.0 37.0 27.0 37.0 28-29 34.592272105389355 37.0 37.0 37.0 29.5 37.0 30-31 34.29493716413489 37.0 37.0 37.0 27.0 37.0 32-33 34.047839179678135 37.0 34.5 37.0 27.0 37.0 34-35 33.76086404603732 37.0 34.5 37.0 24.5 37.0 36-37 33.66281259256125 37.0 34.5 37.0 24.5 37.0 38-39 31.994643789052034 37.0 29.5 37.0 13.0 37.0 40-41 28.047645241822874 34.5 17.5 37.0 8.0 37.0 42-43 29.14387367945951 37.0 20.0 37.0 8.0 37.0 44-45 31.679124529259933 37.0 29.5 37.0 13.0 37.0 46-47 29.606528300822298 37.0 24.5 37.0 10.5 37.0 48-49 29.319937516749178 34.5 20.0 37.0 10.5 37.0 50-51 28.70810942326408 34.5 17.5 37.0 8.0 37.0 52-53 28.044030945429412 34.5 20.0 37.0 10.5 37.0 54-55 27.286090071792266 32.0 17.5 37.0 10.5 37.0 56-57 28.129215503744764 34.5 17.5 37.0 8.0 37.0 58-59 28.5511925415027 34.5 20.0 37.0 10.5 37.0 60-61 29.65542884949012 37.0 20.0 37.0 10.5 37.0 62-63 33.18408933835456 37.0 32.0 37.0 22.0 37.0 64-65 33.70715383855908 37.0 34.5 37.0 24.5 37.0 66-67 33.66897981635848 37.0 32.0 37.0 22.0 37.0 68-69 33.014876796569766 37.0 32.0 37.0 17.5 37.0 70-71 29.401913285095702 34.5 22.5 37.0 13.0 37.0 72-73 30.8728896035205 37.0 29.5 37.0 13.0 37.0 74-75 32.54157322388186 37.0 32.0 37.0 13.0 37.0 76 33.01378016615185 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.8454939763577691 11101 2 -1.3152670626474823 11101 3 -0.48417639482878627 11101 4 -0.7533033086744361 11101 5 -1.131701500436332 11101 6 -1.393190906491263 11101 7 0.044466928955202434 11101 8 -1.896520882268888 11101 9 -1.690433756908753 11101 10-11 -1.4917404402058452 11101 12-13 -0.19819716258355413 11101 14-15 -0.37408242426892 11101 16-17 -0.7349153221888471 11101 18-19 0.19770884863886806 11101 20-21 -0.07617953178559844 11101 22-23 -0.5208293182951564 11101 24-25 -0.23410903756556678 11101 26-27 -0.770271450271288 11101 28-29 -0.22589605901551124 11101 30-31 -0.6744083371680532 11101 32-33 0.2083552257496848 11101 34-35 -0.8155128232382083 11101 36-37 -0.7115627438618617 11101 38-39 -1.2733549897492047 11101 40-41 -3.4505078220376113 11101 42-43 -1.3974975023346765 11101 44-45 -1.4294015412390628 11101 46-47 -1.4100399049138623 11101 48-49 -3.0677673130585497 11101 50-51 -2.479337374478309 11101 52-53 -0.20561556471125542 11101 54-55 -2.464354254629729 11101 56-57 -1.8817249496690067 11101 58-59 -1.5501323925161756 11101 60-61 -0.3286789068496425 11101 62-63 -0.01883845646369764 11101 64-65 -0.29126333716931185 11101 66-67 -0.220134753159698 11101 68-69 -0.5890403260180292 11101 70-71 -1.3556574083885025 11101 72-73 -0.5378327680420867 11101 74-75 -0.4102390144871606 11101 76 -1.3355689028329216 11102 1 -0.6951117498885857 11102 2 1.2382446430381364 11102 3 -1.1289446700318422 11102 4 -1.651057728167988 11102 5 -1.1646685334033648 11102 6 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0.8569052891854128 11103 46-47 -0.26206533046870106 11103 48-49 0.7234414781502387 11103 50-51 0.8693482536190835 11103 52-53 0.4039513389732967 11103 54-55 0.8077315027501442 11103 56-57 1.1637932576139036 11103 58-59 0.15364912008886478 11103 60-61 0.02032992745331086 11103 62-63 -0.2650677173988427 11103 64-65 0.14156363977531328 11103 66-67 -0.3997296680487281 11103 68-69 -1.0837074236263078 11103 70-71 0.43948372498792665 11103 72-73 0.7774548854808572 11103 74-75 0.8163285356098058 11103 76 0.41448496486800224 11104 1 0.23209510123130883 11104 2 -1.685119432499853 11104 3 0.5607786501262595 11104 4 -0.14468970006083026 11104 5 0.3706850019501715 11104 6 -0.8329733462737039 11104 7 -0.23492035043207693 11104 8 0.14343915769115156 11104 9 0.24929318281818524 11104 10-11 0.4443789959135884 11104 12-13 0.9848975205111259 11104 14-15 -0.39123194141843953 11104 16-17 0.975596388322856 11104 18-19 -0.5962749453449163 11104 20-21 0.08710163149556394 11104 22-23 0.9570261595603213 11104 24-25 -0.2984336018901317 11104 26-27 -0.24213292213276105 11104 28-29 -0.5041733372927908 11104 30-31 -1.2930674558271775 11104 32-33 -0.2646717472772835 11104 34-35 -0.5930131007384887 11104 36-37 -0.8379918702909848 11104 38-39 -0.4250921414863562 11104 40-41 -0.19826006978985689 11104 42-43 0.8939887891516136 11104 44-45 -1.587687699525219 11104 46-47 -0.42175041662437707 11104 48-49 -0.29171003700127685 11104 50-51 -0.1804140755550101 11104 52-53 -0.9421568012524908 11104 54-55 -0.3100641003395772 11104 56-57 -0.9596470275910889 11104 58-59 -0.524158366542153 11104 60-61 -0.22877880694953845 11104 62-63 0.3343083966831557 11104 64-65 -0.3232313691373463 11104 66-67 -0.2592067922317369 11104 68-69 -0.6658524028301116 11104 70-71 -1.6188941716252678 11104 72-73 -0.33142806163738214 11104 74-75 0.5694479651998208 11104 76 -1.5522411195051404 11105 1 0.060377929514135786 11105 2 -0.7558265032069222 11105 3 -0.48972640037879245 11105 4 -0.033578588949719546 11105 5 1.23937187063704 11105 6 0.520562007261649 11105 7 -0.21471833023005615 11105 8 0.05253006678205807 11105 9 0.7341416676666697 11105 10-11 -1.5808735293389304 11105 12-13 -0.17671864110503321 11105 14-15 -0.4215349717214707 11105 16-17 0.157414570141043 11105 18-19 -0.48516383423380915 11105 20-21 -0.5745145301205952 11105 22-23 -0.16418596165180332 11105 24-25 -0.6873224907790174 11105 26-27 -0.39869857869841496 11105 28-29 -0.7971026302220849 11105 30-31 -0.5001381628978834 11105 32-33 -0.19396467657021788 11105 34-35 -0.946548454273838 11105 36-37 -1.1208201531192685 11105 38-39 0.11531189891768179 11105 40-41 0.2966894251596379 11105 42-43 -0.595910200747376 11105 44-45 -0.10788971972723971 11105 46-47 0.5630980682241109 11105 48-49 -0.3523160976073356 11105 50-51 1.4205960254550938 11105 52-53 0.8457219866262946 11105 54-55 1.851552061276582 11105 56-57 0.015100447156388697 11105 58-59 1.107154764770982 11105 60-61 -1.1227182008889365 11105 62-63 -0.473772411397654 11105 64-65 -0.7222212681272424 11105 66-67 -1.9864795195044636 11105 68-69 -0.4183776553553571 11105 70-71 -0.3714194241505204 11105 72-73 -0.18496341517273862 11105 74-75 -0.6578247620729094 11105 76 -0.936079503343521 11106 1 -0.27955991042370343 11106 2 2.002040629660211 11106 3 -0.5528577135101038 11106 4 -0.24094067131180097 11106 5 0.9026391589043286 11106 6 -1.5854985987989565 11106 7 -0.36487373038546167 11106 8 -1.863553849301855 11106 9 -1.0132359547109573 11106 10-11 -0.22937780284321008 11106 12-13 -0.7641312285176198 11106 14-15 -1.5334230836095841 11106 16-17 -0.45194828922181784 11106 18-19 0.26158247501250287 11106 20-21 -2.0123059054119707 11106 22-23 -1.4919831644490031 11106 24-25 -0.5637793672358953 11106 26-27 -0.5134033184031566 11106 28-29 -0.7993850700045257 11106 30-31 -0.45050723826695815 11106 32-33 -0.5059304885360234 11106 34-35 0.04849816577277721 11106 36-37 -0.8097770295761464 11106 38-39 -1.07417916557338 11106 40-41 -1.5981726572024435 11106 42-43 -0.09323926057643561 11106 44-45 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64-65 0.8686878227818511 11108 66-67 -0.014762347787296903 11108 68-69 1.0659657789880725 11108 70-71 0.11595431322321659 11108 72-73 1.3205921403828214 11108 74-75 1.7356095813614374 11108 76 0.8831124158483945 11109 1 0.9784349037508875 11109 2 1.4505064896429225 11109 3 0.20837824154607532 11109 4 -0.8523278796091205 11109 5 -0.31073594913370073 11109 6 -0.22033005947311324 11109 7 -0.22334391188934433 11109 8 0.28337787066357123 11109 9 0.424642178391899 11109 10-11 0.10327128963734822 11109 12-13 0.48824560812887796 11109 14-15 0.23321477291591464 11109 16-17 0.5478356347194122 11109 18-19 0.07300209471863184 11109 20-21 0.03852598741430313 11109 22-23 0.422126602188861 11109 24-25 0.40596998453592903 11109 26-27 0.31881362195984764 11109 28-29 0.11272599308968267 11109 30-31 0.5299378784141098 11109 32-33 -0.5435277227624766 11109 34-35 0.8290729280891114 11109 36-37 0.46685434466758835 11109 38-39 0.9008640496383791 11109 40-41 1.0678277749496736 11109 42-43 1.341667826718293 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1.0623034121169113 21612 16-17 1.1048893176157932 21612 18-19 0.852714953644977 21612 20-21 0.6527581971521315 21612 22-23 0.6565211090552694 21612 24-25 0.9879300344735071 21612 26-27 0.2098872798874396 21612 28-29 0.683200400080942 21612 30-31 1.0544072916475713 21612 32-33 0.7671464345408978 21612 34-35 0.6393101315847431 21612 36-37 0.7155434832443675 21612 38-39 0.996119979725762 21612 40-41 2.2280025564727666 21612 42-43 2.165200910363737 21612 44-45 1.561807249969732 21612 46-47 1.2186536237796624 21612 48-49 0.612330367039128 21612 50-51 0.6392828941419602 21612 52-53 1.0421866330909424 21612 54-55 1.7394308491553723 21612 56-57 -0.25712177506583345 21612 58-59 1.326851734467951 21612 60-61 0.4575848294140954 21612 62-63 -0.08892392654917103 21612 64-65 -0.5590899549959261 21612 66-67 -0.6369845700095169 21612 68-69 0.21041022343251825 21612 70-71 1.093732091000998 21612 72-73 1.0761476959383742 21612 74-75 0.9744984702503174 21612 76 0.24977908251506165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 9.0 18 21.0 19 63.0 20 210.0 21 464.0 22 978.0 23 1861.0 24 2776.0 25 3925.0 26 5187.0 27 6429.0 28 7801.0 29 9369.0 30 11624.0 31 14721.0 32 17386.0 33 19625.0 34 19238.0 35 15222.0 36 4888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.105350014810217 10.491276076561773 10.335416167115676 56.06795774151233 2 17.844901758889655 31.076068098791204 35.356926245116156 15.722103897202985 3 17.915173911816975 18.130976896386358 52.57341532906429 11.380433862732376 4 15.658189819320443 54.10725116010099 17.510120029901692 12.724438990676878 5 15.687104190468132 32.70497468229453 44.536594310215946 7.071326817021396 6 20.71426556461395 60.929081215267 11.58001636153347 6.776636858585573 7 19.68575015162414 18.998857529725385 10.273769728769095 51.041622589881385 8 19.19420584211343 59.299143852522604 7.76597695313051 13.74067335223346 9 19.543293981579428 12.93389187435648 7.2250666440993525 60.297747499964736 10-11 19.707045988279017 36.05330155080855 31.390297396983012 12.849355063929421 12-13 19.999929477143542 10.771308481078718 34.714170862776626 34.514591179001116 14-15 19.80422855047321 9.827360047391359 12.210327367099676 58.15808403503575 16-17 44.37509696892763 32.10482517383884 8.316055233501178 15.204022623732353 18-19 45.30792150778399 14.803152382799414 8.62003914032335 31.268886969093252 20-21 20.90015373982708 10.502969012256873 8.55406987404618 60.04280737386986 22-23 19.86678138465553 35.476067899885045 8.568587487746568 36.088563227712854 24-25 44.28963858650615 34.26498954443351 8.559459202132725 12.885912666927615 26-27 45.41276799853282 37.579222464792956 8.678585158198048 8.329424378476178 28-29 20.0801258314113 37.407336874105106 9.64613442236752 32.86640287211607 30-31 19.238269885211505 40.46197520850598 30.894856558461026 9.404898347821486 32-33 19.17606773707801 59.28122806955676 13.121413986817931 8.421290206547306 34-35 43.5603936794073 38.014535529531244 9.703047806446834 8.722022984614625 36-37 20.18822550388581 36.435986403193276 9.862621475620248 33.51316661730067 38-39 18.998152301160808 15.374335322077886 33.94794002736287 31.679572349398438 40-41 19.036637399062027 13.926090482739165 33.98039423110829 33.05687788709052 42-43 43.41161536020311 11.199266546775274 32.93733911632991 12.451778976691703 44-45 20.077857233529386 36.092540092243894 34.69971367720278 9.129888997023935 46-47 42.85915785984548 12.284413601469742 14.986229975280954 29.87019856340382 48-49 40.936402488046376 10.323488342571828 10.980761364758319 37.75934780462348 50-51 21.89346817303488 13.452940097885724 34.301259538216335 30.352332190863056 52-53 18.203359708881646 17.219918475577938 52.12450105079056 12.452220764749855 54-55 18.09244396800993 19.770582676276852 32.089157510190844 30.04781584552238 56-57 42.23719657541009 12.109127068082765 14.277704904159439 31.375971452347706 58-59 19.385585375916385 18.140817456371412 49.60065165187403 12.872945515838175 60-61 41.78161892269284 34.37989252316676 15.028068096870195 8.810420457270201 62-63 18.91676510516758 62.84772298524304 9.106295950210688 9.129215959378692 64-65 17.65796962441331 64.8349865824115 8.399657243205692 9.107386549969497 66-67 17.651954060277372 64.66259269576746 8.304976568202575 9.380476675752586 68-69 18.60710306210243 63.81930633718388 8.313586933525155 9.260003667188537 70-71 36.10699727781774 46.263346450584635 8.344616990366578 9.285039281231047 72-73 70.59815015286098 11.910462602832952 8.23157293426096 9.259814310045101 74-75 72.92792157415897 9.905494040482402 8.130686226109034 9.035898159249594 76 73.19806477093206 9.953311893477771 7.836126156623786 9.012497178966374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 2.0 12 3.0 13 3.5 14 4.0 15 7.0 16 9.5 17 9.0 18 18.5 19 22.5 20 35.5 21 54.0 22 55.0 23 83.5 24 156.5 25 202.0 26 221.5 27 274.5 28 388.0 29 468.0 30 517.5 31 729.0 32 1010.5 33 1130.0 34 1268.5 35 1592.0 36 2034.0 37 2291.0 38 2639.0 39 3482.0 40 4583.0 41 6242.0 42 7295.0 43 9592.5 44 17465.0 45 30013.5 46 36987.0 47 20957.0 48 4241.5 49 3141.5 50 2727.0 51 2457.5 52 2002.0 53 1642.0 54 1468.0 55 1402.0 56 1260.5 57 1054.5 58 924.0 59 835.0 60 683.0 61 579.0 62 538.0 63 493.5 64 443.0 65 393.5 66 306.0 67 262.0 68 266.0 69 265.0 70 232.5 71 205.0 72 183.0 73 162.5 74 139.0 75 114.0 76 121.5 77 128.5 78 97.5 79 67.0 80 71.0 81 67.0 82 47.0 83 35.0 84 26.0 85 17.5 86 13.5 87 9.0 88 9.5 89 13.0 90 14.0 91 12.5 92 13.0 93 14.0 94 15.5 95 31.5 96 47.0 97 67.5 98 237.5 99 7698.5 100 15010.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0028209142583111185 2 0.0028209142583111185 3 0.0014104571291555592 4 0.0 5 0.0 6 0.0014104571291555592 7 0.0 8 0.0 9 0.0 10-11 7.052285645777796E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.526142822888898E-4 20-21 0.0 22-23 7.052285645777796E-4 24-25 0.005289214234333347 26-27 0.0208042426550445 28-29 0.014809799856133373 30-31 0.010931042750955584 32-33 0.010225814186377805 34-35 0.005289214234333347 36-37 0.0 38-39 0.0 40-41 0.002115685693733339 42-43 0.002115685693733339 44-45 0.0 46-47 0.0031735285406000084 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0028209142583111185 56-57 0.0 58-59 0.0031735285406000084 60-61 0.0 62-63 3.526142822888898E-4 64-65 0.0045839856697555675 66-67 0.0024682999760222285 68-69 0.0 70-71 0.0 72-73 0.001763071411444449 74-75 0.005641828516622237 76 0.004231371387466678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 141798.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.230327649191103 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96696682685578 22.965062976910815 2 10.654131479494238 6.441557708853439 3 5.384220594410488 4.883002581136546 4 3.032706573974712 3.667188535804454 5 1.8079596883310782 2.732760687738896 6 1.0334530863621518 1.8744975246477384 7 0.5715485466336957 1.2094669882508922 8 0.3522605328232165 0.8519161060099579 9 0.1936266504922316 0.5268057377396014 >10 0.8141650725516727 4.399215785836189 >50 0.08164979237624224 1.6876119550346267 >100 0.08398264358699202 5.4598795469611705 >500 0.009331404842999113 1.9831027235927163 >1k 0.006998553632249335 6.500796908277973 >5k 0.0023328512107497782 6.910534704297663 >10k+ 0.0046657024214995565 27.906599528907318 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 24295 17.133527976417156 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15276 10.773071552490162 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 9799 6.910534704297663 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 4275 3.014852113570008 No Hit CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 2562 1.8067955824482715 No Hit CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 2381 1.6791492122596934 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 885 0.624127279651335 No Hit CAAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 736 0.5190482235292458 No Hit CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 644 0.4541671955880901 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 547 0.3857600248240455 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT 488 0.3441515395139565 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT 461 0.32511036827035644 No Hit CAAAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 405 0.28561756865400073 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT 395 0.278565283008223 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT 366 0.25811365463546737 No Hit ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT 349 0.2461247690376451 RNA PCR Primer, Index 41 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT 317 0.22355745497115614 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT 301 0.21227379793791168 No Hit TCTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 290 0.2045162837275561 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT 277 0.19534831238804495 No Hit CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT 246 0.1734862268861338 No Hit CTAAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 235 0.1657287126757782 No Hit CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC 235 0.1657287126757782 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT 217 0.1530345985133782 No Hit CACTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 204 0.14386662717386706 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 189 0.13328819870520034 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 189 0.13328819870520034 No Hit CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT 181 0.1276463701885781 No Hit CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT 172 0.1212993131073781 No Hit CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT 162 0.11424702746160031 No Hit ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT 158 0.11142611320328918 No Hit CATAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT 153 0.10789997038040029 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG 151 0.10648951325124474 No Hit CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT 146 0.10296337042835584 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 143 0.1008476847346225 No Hit CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 142 0.1001424561700447 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007757514210355576 0.0 2 0.0 0.0 0.0 0.008462742774933356 0.0 3 0.0 0.0 0.0 0.009873199904088916 0.0 4 0.0 0.0 0.0 0.01974639980817783 0.0 5 0.0 0.0 0.0 0.025388228324800068 0.0 6 0.0 0.0 0.0 0.025388228324800068 0.0 7 0.0 0.0 0.0 0.025388228324800068 0.0 8 0.0 0.0 0.0 0.025388228324800068 0.0 9 0.0 0.0 0.0 0.026798685453955626 0.0 10 0.0 0.0 0.0 0.028209142583111187 0.0 11 0.0 0.0 0.0 0.029619599712266746 0.0 12 0.0 0.0 0.0 0.029619599712266746 0.0 13 0.0 0.0 0.0 0.029619599712266746 0.0 14 0.0 0.0 0.0 0.031030056841422304 0.0 15 0.0 0.0 0.0 0.031030056841422304 0.0 16 0.0 0.0 0.0 0.031030056841422304 0.0 17 0.0 0.0 0.0 0.03173528540600008 0.0 18 0.0 0.0 0.0 0.03314574253515564 0.0 19 0.0 0.0 0.0 0.03737711392262232 0.0 20 0.0 0.0 0.0 0.038082342487200105 0.0 21 0.0 0.0 0.0 0.04090325674551122 0.0 22 0.0 0.0 0.0 0.05148168521417792 0.0 23 0.0 0.0 0.0 0.05923919942453349 0.0 24 0.0 0.0 0.0 0.06276534224742239 0.0 25 0.0 0.0 0.0 0.06488102794115573 0.0 26 0.0 0.0 0.0 0.0655862565057335 0.0 27 0.0 0.0 0.0 0.083216970620178 0.0 28 0.0 0.0 0.0 0.11283657033244475 0.0 29 0.0 0.0 0.0 0.12412022736568921 0.0 30 0.0 0.0 0.0 0.1495084556904893 0.0 31 0.0 0.0 0.0 0.1657287126757782 0.0 32 0.0 0.0 0.0 0.1911169410005783 0.0 33 0.0 0.0 0.0 0.23413588343982283 0.0 34 0.0 0.0 0.0 0.2708077687978674 0.0 35 0.0 0.0 0.0 0.3575508822409343 0.0 36 0.0 0.0 0.0 0.46333516692760124 0.0 37 0.0 0.0 0.0 0.6629148507031128 0.0 38 0.0 0.0 0.0 0.9076291626116024 0.0 39 0.0 0.0 0.0 1.1727951028928476 0.0 40 0.0 0.0 0.0 1.4823904427424928 0.0 41 0.0 0.0 0.0 1.8089112681420048 0.0 42 0.0 0.0 0.0 2.2546157209551616 0.0 43 0.0 0.0 0.0 2.641080974343785 0.0 44 0.0 0.0 0.0 2.9676017997432966 0.0 45 0.0 0.0 0.0 3.302585367917742 0.0 46 0.0 0.0 0.0 3.68129310709601 0.0 47 0.0 0.0 0.0 3.987362304122766 0.0 48 0.0 0.0 0.0 4.332924300765878 0.0 49 0.0 0.0 0.0 4.624888926501079 0.0 50 0.0 0.0 0.0 4.915443095107125 0.0 51 0.0 0.0 0.0 5.287098548639614 0.0 52 0.0 0.0 0.0 5.655227859349215 0.0 53 0.0 0.0 0.0 5.991621884652816 0.0 54 0.0 0.0 0.0 6.26031396775695 0.0 55 0.0 0.0 0.0 6.584013878898151 0.0 56 0.0 0.0 0.0 6.858347790518907 0.0 57 0.0 0.0 0.0 7.067800674198508 0.0 58 0.0 0.0 0.0 7.319567271752775 0.0 59 0.0 0.0 0.0 7.517031269834553 0.0 60 0.0 0.0 0.0 7.769503095953398 0.0 61 0.0 0.0 0.0 8.01351217929731 0.0 62 0.0 0.0 0.0 8.2391853199622 0.0 63 0.0 0.0 0.0 8.498709431726823 0.0 64 0.0 0.0 0.0 8.716625058181357 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTTA 15 0.0022209603 69.9993 10 AGACTCG 30 1.0178883E-7 69.9993 25 AGAAGCG 15 0.0022209603 69.9993 5 GCGTTAT 15 0.0022209603 69.9993 11 CGTTATG 15 0.0022209603 69.9993 12 TCTACAG 15 0.0022209603 69.9993 50 GAGCGTT 15 0.0022209603 69.9993 9 TCGCTCT 15 0.0022209603 69.9993 46 AGTCTTA 15 0.0022209603 69.9993 48 ACGTCAA 15 0.0022209603 69.9993 47 CTACAGC 15 0.0022209603 69.9993 51 CTCGAAG 30 1.0178883E-7 69.9993 28 AGCCTAT 15 0.0022209603 69.9993 30 TATAACG 15 0.0022209603 69.9993 43 ATACCGA 15 0.0022209603 69.9993 44 AACACGT 15 0.0022209603 69.9993 37 GTATTAA 40 1.3460522E-10 69.99929 49 ACACCGA 20 7.905096E-5 69.99929 11 TTCCGTA 20 7.905096E-5 69.99929 45 TAACGTA 20 7.905096E-5 69.99929 38 >>END_MODULE