FastQCFastQC Report
Wed 25 May 2016
SRR1780161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87433
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5243259.968204224949396No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG87109.961913693914196No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT8220.9401484565324305No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG4510.5158235448857983No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC4060.4643555636887674No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG2940.33625747715393506No Hit
CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG960.10979835988699918No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG950.1086546269715096No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG940.10751089405602005No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT150.00221801470.027
CTTTATA401.3278623E-1070.02
CTATACA301.01324986E-770.01
CATCTGG150.00221801470.09
GAGTCCT150.00221801470.058
GGCCCGA150.00221801470.010
GTCCTAG150.00221801470.060
TGTGGCG150.00221801470.011
GTGTGGC150.00221801470.010
ATCTGGC150.00221801470.010
TGAGTCC150.00221801470.057
TGTGCGC150.00221801470.03
ATATAGC150.00221801470.052
TCTTTAT453.783498E-1062.2222251
AAAAAGG6000.060.0833370
ATCATTA12200.053.64753759
TCATTAA12250.053.4285760
ATTAAAA12400.053.34677562
GCCGTAT12350.053.27935454
TATCATT12250.053.14285758
Produced by FastQC (version 0.11.5)