SRR1780161_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780161_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87433
Sequences flagged as poor quality	0
Sequence length	76
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	19672	22.499513913510917	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	12691	14.515114430478196	No Hit
CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	9298	10.634428648222068	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	3639	4.162044079466563	No Hit
CATAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	2584	2.9554058536250616	No Hit
CTTAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	2314	2.6465979664428763	No Hit
CAAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	841	0.9618793819267325	No Hit
CTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	708	0.809762904166619	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC	614	0.702252010110599	No Hit
CAAAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	567	0.648496563082589	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC	542	0.6199032401953496	No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT	424	0.48494275616757976	Illumina PCR Primer Index 5 (95% over 22bp)
CTAAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT	348	0.39801905459037207	No Hit
TCTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	324	0.3705694646186222	No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC	242	0.2767833655484771	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT	207	0.236752713506342	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	179	0.2047281918726339	No Hit
CACTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC	163	0.1864284652248007	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC	159	0.1818535335628424	No Hit
CATATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT	158	0.18070980064735284	No Hit
TATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCT	154	0.17613486898539452	Illumina PCR Primer Index 5 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	154	0.17613486898539452	No Hit
TCATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	139	0.1589788752530509	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT	137	0.15669140942207177	No Hit
CTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG	122	0.13953541568972813	No Hit
CTTTACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC	117	0.13381675111228025	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT	109	0.12466688778836367	No Hit
AAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT	107	0.12237942195738451	Illumina PCR Primer Index 5 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCG	103	0.11780449029542622	No Hit
CATATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT	88	0.1006484965630826	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	25	2.8190752E-6	70.00001	1
TGATTGA	25	2.8190752E-6	70.00001	55
CTACGAG	25	2.8190752E-6	70.00001	12
ACACGTA	20	7.887767E-5	70.0	38
AAATACC	15	0.002218014	70.0	2
TCTACGA	20	7.887767E-5	70.0	11
TCTATAT	15	0.002218014	70.0	1
AACCAAT	20	7.887767E-5	70.0	29
ATAGATA	15	0.002218014	70.0	33
TTGAACA	15	0.002218014	70.0	34
AATACCC	20	7.887767E-5	70.0	3
ATTCTGC	15	0.002218014	70.0	51
GACCCCC	20	7.887767E-5	70.0	26
TGAACAC	20	7.887767E-5	70.0	35
AGATATC	15	0.002218014	70.0	35
AACACGT	15	0.002218014	70.0	37
TTGCCCA	15	0.002218014	70.0	33
ATAAACA	420	0.0	68.33333	2
CTTAAAC	350	0.0	67.00001	1
TTAAACA	350	0.0	67.00001	2

Summary