FastQCFastQC Report
Wed 25 May 2016
SRR1780160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101497
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6653965.55760268776417No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG1397713.770850369961673No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT13031.2837817866537928No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG5920.5832684709892904No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC4470.4404071056287378No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG3910.3852330610756968No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2780.27389972117402483No Hit
CTTATACACATCTGACGCTGCCGACGACGATGCTGGTGTAGATCTCGGTG1420.13990561297378248No Hit
CTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT1410.13892036217819245No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.12315634944875219No Hit
CTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGG1190.11724484467521207No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150.113303841492852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG150.002219045870.02
TAGTGTA207.893879E-570.034
GCTAGTG150.002219045870.032
TGCGCGT207.893879E-570.05
TATGCGC150.002219045870.03
GATGCTG150.002219045870.029
GGTCTCG150.002219045870.041
AAGAGGA150.002219045870.024
GCGCGTC150.002219045870.06
GGACACA150.002219045870.01
AGGTGTA207.893879E-570.034
TCACGGT150.002219045870.043
AGTGTAG207.893879E-570.035
TTCTTAT150.002219045870.01
ATGCTGG150.002219045870.030
GTCTCGG150.002219045870.042
GACGATG301.01488695E-770.026
GCGTCTG207.893879E-570.08
GACGACC150.002219045870.023
CTAGTGT207.893879E-570.033
Produced by FastQC (version 0.11.5)