FastQCFastQC Report
Wed 25 May 2016
SRR1780160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101497
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2654726.155452870528194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1996319.668561632363517No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1133211.164862015626078No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG46734.604076967792151No Hit
CATAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT35233.4710385528636314No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT31673.1202892696335853No Hit
CAAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT11781.1606254372050406No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT9500.9359882558105166No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC8350.8226844143176646No Hit
CAAAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT8250.8128319063617644No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC7180.7074100712336325No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT5860.5773569662157503TruSeq Adapter, Index 8 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT4870.4798171374523385No Hit
TCTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG4210.4147905849433973No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC3160.3113392514064455No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2360.23251918775924413No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC2300.226607682985704No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2150.21182892105185377No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC2070.2039469146871336No Hit
CTTTACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2020.1990206607091835No Hit
TATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCT1840.18128614638856322No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT1760.17340414002384308No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1600.1576401272944028No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT1580.15566962570322276No Hit
TCATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1420.13990561297378248No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT1330.1310383558134723No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAATCTCGTATGCCGTC1280.12611210183552224No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAGT1180.11625959387962206No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAGT1180.11625959387962206No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAATTAAATCTCGTATGCCGT1170.11527434308403203No Hit
AAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT1120.11034808910608196RNA PCR Primer, Index 8 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT1020.10049558115018178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAAA353.6561687E-970.0310727
AAGATTT207.8765326E-570.0310728
AGAAATA207.8765326E-570.0310729
ATTAAAT750.070.0310732
CAGAAAT207.8765326E-570.0310728
TATAACT150.00221514270.0310632
TATAAAA150.00221514270.0310632
CAGAAAA150.00221514270.0310628
ACTGCTT500.069.9965553
CTTCAAA150.002219480269.9965557
AACGTAT150.002219480269.9965539
CTATATA207.895806E-569.996552
AATCTGC207.895806E-569.9965551
AACAAAG150.002219480269.9965565
TAAACCC301.01525075E-769.996553
AAATCTA150.002219480269.9965535
TATATAC252.8231789E-669.996553
AACGAGC252.8231789E-669.9965513
ATGACGA207.895806E-569.9965544
AAACACC252.8231789E-669.996554
Produced by FastQC (version 0.11.5)